FastQCFastQC Report
Sat 18 Jun 2016
SRR578579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10722184
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131797112.292001331072102No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT294780.27492533237631434No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG109730.1023392249191023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2875400.091.5851065
AAGCAGT2900650.091.3835451
GTATCAA2903250.091.231439
TGGTATC2891150.091.145497
GGTATCA2904200.091.074098
GTGGTAT2914300.090.544256
AGCAGTG2927000.090.470592
GCAGTGG2927600.090.292833
CAGTGGT2928150.090.0158844
GCGGTAT12600.047.1461266
TCAACGC2896350.046.2871812-13
AACGCAG2901850.046.2457914-15
CAACGCA2891650.046.1545612-13
ATCAACG2891600.046.14869310-11
CGCAGAG2915600.046.00877416-17
ACGCAGA2909750.045.9275514-15
TATCAAC2913550.045.911110-11
CAGAGTA2894550.045.7349118-19
AGAGTAC2844600.045.59781618-19
GAGTACT1676550.045.48861320-21
Produced by FastQC (version 0.11.5)