FastQCFastQC Report
Sat 18 Jun 2016
SRR578579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10722184
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430611.3342524246925813No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236010.22011373802202985No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107950.10067911537425583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4050.032.823963
CGCGTAG2300.028.90003454-55
TCGCGTA2650.024.18936752-53
CAACGCA79150.019.79396412-13
ACGTACG3650.019.51067220-21
CGTACGC3900.019.4773220-21
ATCAACG82900.019.04179610-11
CTCGCGT3550.018.72562452-53
TCAACGC80050.017.5253610-11
ACTCGCG3850.017.26672650-51
AACGCAG84900.016.63600512-13
AGTGGTA98850.016.4258295
GGTATCA106750.015.6102928
GTATCAA109000.015.2445069
AAGCAGT113550.015.1188761
TGGTATC114000.014.74253757
ACGCAGA109850.014.19727814-15
CGCTAGT4750.013.99376690-91
ATCGGAA17800.013.6031082
TATCAAC117750.013.3053059
Produced by FastQC (version 0.11.5)