##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578579_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10722184 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.387709164476192 31.0 31.0 34.0 30.0 34.0 2 31.763042212295556 31.0 31.0 34.0 30.0 34.0 3 31.83667226751565 31.0 31.0 34.0 30.0 34.0 4 35.5428265360863 37.0 35.0 37.0 33.0 37.0 5 35.36775017104724 37.0 35.0 37.0 33.0 37.0 6 35.361697579522975 37.0 35.0 37.0 32.0 37.0 7 35.3543091594026 37.0 35.0 37.0 32.0 37.0 8 35.311680157699215 37.0 35.0 37.0 32.0 37.0 9 36.85021866813701 39.0 37.0 39.0 32.0 39.0 10-11 36.844827648919285 39.0 37.0 39.0 32.0 39.0 12-13 36.834663721495545 39.0 37.0 39.0 32.0 39.0 14-15 38.00481501716441 40.0 37.0 41.0 33.0 41.0 16-17 37.9368164172523 40.0 37.0 41.0 33.0 41.0 18-19 37.86401618364319 40.0 37.0 41.0 32.0 41.0 20-21 37.821319425221574 40.0 37.0 41.0 32.0 41.0 22-23 37.62012776501504 39.5 36.0 41.0 32.0 41.0 24-25 37.6176869842935 39.0 36.0 41.0 32.0 41.0 26-27 37.44440129921293 39.0 36.0 41.0 32.0 41.0 28-29 37.29207146603714 39.0 36.0 41.0 31.0 41.0 30-31 37.15042746888134 39.0 36.0 41.0 31.0 41.0 32-33 37.00994004579664 39.0 36.0 40.5 31.0 41.0 34-35 36.8611917124347 39.0 35.5 40.0 30.0 41.0 36-37 36.70635758535761 39.0 35.0 40.0 30.0 41.0 38-39 36.533358642231846 38.0 35.0 40.0 30.0 41.0 40-41 36.37321081227481 38.0 35.0 40.0 30.0 41.0 42-43 36.45493861138738 38.0 35.0 40.0 30.0 41.0 44-45 36.38384996937191 38.0 35.0 40.0 30.0 41.0 46-47 36.23467369147927 38.0 35.0 40.0 30.0 41.0 48-49 36.08099483276915 38.0 35.0 40.0 29.0 41.0 50-51 35.892030159154146 38.0 34.5 40.0 28.5 41.0 52-53 35.93290117013474 38.0 35.0 40.0 28.0 41.0 54-55 35.920103450938726 38.0 34.5 40.0 28.0 41.0 56-57 35.75855292168088 38.0 34.0 40.0 28.0 41.0 58-59 35.528593987941264 38.0 34.0 40.0 28.0 41.0 60-61 35.26951617319755 38.0 34.0 40.0 28.0 41.0 62-63 34.9850346720407 37.0 34.0 40.0 27.0 41.0 64-65 34.66175897559676 37.0 33.0 40.0 26.5 41.0 66-67 34.31911208574671 36.0 33.0 39.0 26.0 41.0 68-69 33.9408043174786 36.0 32.5 39.0 26.0 40.5 70-71 33.54715862924942 35.0 32.0 39.0 26.0 40.0 72-73 33.15318278440288 35.0 32.0 38.0 25.5 40.0 74-75 32.74118397893564 35.0 31.5 37.0 25.0 39.5 76-77 31.36926306245071 34.0 29.5 36.0 23.0 39.0 78-79 32.02668057179395 34.5 31.0 36.0 24.0 39.0 80-81 31.90235627368454 35.0 31.0 36.0 24.0 38.5 82-83 31.540242127909764 34.0 31.0 35.5 23.0 37.0 84-85 31.257585488180393 34.0 31.0 35.0 23.0 37.0 86-87 30.93804830247271 34.0 31.0 35.0 21.5 36.5 88-89 30.62698303815715 34.0 30.5 35.0 20.0 36.0 90-91 30.282007844670453 34.0 30.0 35.0 19.5 36.0 92-93 29.9057534360537 34.0 30.0 35.0 17.5 35.5 94-95 29.520024418532643 34.0 29.5 35.0 10.0 35.0 96-97 29.04885763012461 34.0 29.0 35.0 2.0 35.0 98-99 28.234298208275476 34.0 29.0 35.0 2.0 35.0 100-101 27.173345047986494 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3006245954656812 1101 2 0.23913650271817843 1101 3 0.34736604017894734 1101 4 0.25188516046717524 1101 5 0.2651039385966172 1101 6 0.18317087503387341 1101 7 0.2211379124186479 1101 8 0.1682208642512677 1101 9 0.3150488608390134 1101 10-11 0.2912988213290433 1101 12-13 0.3041880199536777 1101 14-15 0.227560382628468 1101 16-17 0.2737566642304756 1101 18-19 0.37877041661035804 1101 20-21 0.3143361152531128 1101 22-23 0.45191968695952767 1101 24-25 0.3523508198430392 1101 26-27 0.25900442099322873 1101 28-29 0.27690040213784073 1101 30-31 0.3137030413077966 1101 32-33 0.26836095180217967 1101 34-35 0.31620376036466524 1101 36-37 0.30905106792810244 1101 38-39 0.20147960103394524 1101 40-41 0.27666761280556784 1101 42-43 0.22814546221002274 1101 44-45 0.2375248729993089 1101 46-47 0.21581675503538378 1101 48-49 0.23104481149713507 1101 50-51 0.3222318454733113 1101 52-53 0.3256104930967041 1101 54-55 0.33720420243071914 1101 56-57 0.39847828109496675 1101 58-59 0.3920947684698035 1101 60-61 0.30591438399483906 1101 62-63 0.2865340321821819 1101 64-65 0.36928865915950837 1101 66-67 0.42808894734121594 1101 68-69 0.32497136880308375 1101 70-71 0.3152466927913764 1101 72-73 0.4815931181227384 1101 74-75 0.42055119817405995 1101 76-77 0.31154999932071803 1101 78-79 0.37344445361026857 1101 80-81 0.31020320241917787 1101 82-83 0.4475988780563327 1101 84-85 0.43827446890645305 1101 86-87 0.4417696153544277 1101 88-89 0.596746506135652 1101 90-91 0.5071188844457488 1101 92-93 0.49693414416436354 1101 94-95 0.3757799382342526 1101 96-97 0.46566352829087165 1101 98-99 0.8923037953906459 1101 100-101 1.0728716949405772 1102 1 0.3275543356191051 1102 2 0.3246941316679077 1102 3 0.2881544634287394 1102 4 0.2825622470778981 1102 5 0.30015923536979017 1102 6 0.20465721836301043 1102 7 0.24278037613343884 1102 8 0.1088700555864861 1102 9 0.23637565383666725 1102 10-11 0.2891135324824532 1102 12-13 0.3049768515125848 1102 14-15 0.3813801479694092 1102 16-17 0.2745476091809209 1102 18-19 0.31059303515119296 1102 20-21 0.3684887551581397 1102 22-23 0.5003353878855705 1102 24-25 0.40541601949313133 1102 26-27 0.44565740351961125 1102 28-29 0.37628322275958226 1102 30-31 0.42315825338108226 1102 32-33 0.3757054875476342 1102 34-35 0.336490504396572 1102 36-37 0.3626123874099747 1102 38-39 0.33173724333769883 1102 40-41 0.41046538189265647 1102 42-43 0.3262009656383569 1102 44-45 0.3462122054815353 1102 46-47 0.35181841361387 1102 48-49 0.3304692747964637 1102 50-51 0.3438572667613542 1102 52-53 0.37088261040638315 1102 54-55 0.36579808213837595 1102 56-57 0.43166338497081824 1102 58-59 0.4459636120809236 1102 60-61 0.44072177107553756 1102 62-63 0.20695453477114256 1102 64-65 0.232319613095747 1102 66-67 0.32778155290797173 1102 68-69 0.33361421649266276 1102 70-71 0.3141380206491817 1102 72-73 0.44112591079359476 1102 74-75 0.38511281302690037 1102 76-77 0.19118387387636204 1102 78-79 0.2982108356697992 1102 80-81 0.33630222573930624 1102 82-83 0.44909731465044445 1102 84-85 0.3620662462252646 1102 86-87 0.43272218148016606 1102 88-89 0.39722287112159904 1102 90-91 0.4155254652241851 1102 92-93 0.40289504573029333 1102 94-95 0.40727585909932174 1102 96-97 0.5665293479512599 1102 98-99 0.9045108009449194 1102 100-101 0.9475431581942892 1103 1 0.24990121765594253 1103 2 0.27831775829439565 1103 3 0.24939832876035695 1103 4 0.2322570220411606 1103 5 0.24294934626546905 1103 6 0.08848234897849494 1103 7 0.25183703226975496 1103 8 0.044349655596263915 1103 9 0.221778686664436 1103 10-11 0.18799251856318477 1103 12-13 0.25617573474300315 1103 14-15 0.36757429880527326 1103 16-17 0.3161562515810701 1103 18-19 0.2692478005562293 1103 20-21 0.3863629627230907 1103 22-23 0.40330266190105135 1103 24-25 0.37137279715488347 1103 26-27 0.23132386403239735 1103 28-29 0.2143614252567687 1103 30-31 0.29766267396045265 1103 32-33 0.2639682941866184 1103 34-35 0.27941828314515504 1103 36-37 0.32893381093334284 1103 38-39 0.39778322181619075 1103 40-41 0.3479751105865958 1103 42-43 0.2837744668529254 1103 44-45 0.2102997419585435 1103 46-47 0.3060173626465499 1103 48-49 0.30569979638566025 1103 50-51 0.25296134624252176 1103 52-53 0.2310313056033948 1103 54-55 0.3798229076779265 1103 56-57 0.25936441333476523 1103 58-59 0.3055366866742304 1103 60-61 0.32795788366934886 1103 62-63 0.37303270864136806 1103 64-65 0.22385676761155793 1103 66-67 0.19989651679724574 1103 68-69 0.31060276153331046 1103 70-71 0.23919705878725495 1103 72-73 0.2299650934699926 1103 74-75 0.30203170509192745 1103 76-77 0.21476662557544302 1103 78-79 0.3386674416691804 1103 80-81 0.2819165653023852 1103 82-83 0.531896327735943 1103 84-85 0.337583584124598 1103 86-87 0.2888486394920662 1103 88-89 0.3369021855376779 1103 90-91 0.35150151439734856 1103 92-93 0.3078863850804545 1103 94-95 0.4490999492042711 1103 96-97 0.45300172700880736 1103 98-99 0.9336175622157405 1103 100-101 0.8346543260291952 1104 1 0.28581684885202563 1104 2 0.23125216570209162 1104 3 0.1645397109216873 1104 4 0.16944657280857456 1104 5 0.19688134700889748 1104 6 0.14912156665380394 1104 7 0.14660469759525796 1104 8 0.07372799969912336 1104 9 0.255323567636097 1104 10-11 0.12695834816102547 1104 12-13 0.18910465860758308 1104 14-15 0.3092529687377734 1104 16-17 0.3365099690941662 1104 18-19 0.18602397481391364 1104 20-21 0.22543361588978428 1104 22-23 0.35422927289479844 1104 24-25 0.2745669059374336 1104 26-27 0.30669833694431503 1104 28-29 0.31712860612410054 1104 30-31 0.29576528906172683 1104 32-33 0.3215119302512832 1104 34-35 0.20352675147193366 1104 36-37 0.21453046479581417 1104 38-39 0.2751622302355301 1104 40-41 0.3311385451536708 1104 42-43 0.23400616917594874 1104 44-45 0.2071527553090462 1104 46-47 0.1942366734570058 1104 48-49 0.3056047148597756 1104 50-51 0.20723814886876823 1104 52-53 0.19472810278642783 1104 54-55 0.23647966021705003 1104 56-57 0.19739362210834344 1104 58-59 0.19694681315842644 1104 60-61 0.22305858008983392 1104 62-63 0.2181599109439034 1104 64-65 0.20490713814307782 1104 66-67 0.24181132619064982 1104 68-69 0.24873567018497766 1104 70-71 0.21619102431364468 1104 72-73 0.24591904472432446 1104 74-75 0.24538339463979497 1104 76-77 0.18367594115489183 1104 78-79 0.33962322286154034 1104 80-81 0.42405152317279615 1104 82-83 0.38039417860533575 1104 84-85 0.2671155552283544 1104 86-87 0.36107290552454785 1104 88-89 0.4067652857669266 1104 90-91 0.3781102658501396 1104 92-93 0.4504013809285645 1104 94-95 0.44739086301366626 1104 96-97 0.4253302064613145 1104 98-99 0.8573570345637762 1104 100-101 0.9153966341117119 1105 1 0.22238563750640594 1105 2 0.15090566493324076 1105 3 0.09960439080432693 1105 4 0.19299774984830975 1105 5 0.18517460921272288 1105 6 0.06864745538346995 1105 7 0.1430977608609112 1105 8 0.05877712270683588 1105 9 0.20882514667763274 1105 10-11 0.10597295647038862 1105 12-13 0.13812188207050013 1105 14-15 0.12995728345805446 1105 16-17 0.116481052539541 1105 18-19 0.25204932939785607 1105 20-21 0.16714276319070365 1105 22-23 0.2518750560492009 1105 24-25 0.2740733820850423 1105 26-27 0.20829461718187048 1105 28-29 0.22495798538023593 1105 30-31 0.17421838868475703 1105 32-33 0.26354480375552924 1105 34-35 0.21887264903201498 1105 36-37 0.23243240644533358 1105 38-39 0.3078721437656142 1105 40-41 0.24888122553370096 1105 42-43 0.2341659466489503 1105 44-45 0.29785140511217634 1105 46-47 0.2956832255381485 1105 48-49 0.31481793163435867 1105 50-51 0.396708342570804 1105 52-53 0.3849299441842362 1105 54-55 0.4587000396482708 1105 56-57 0.37907713157947853 1105 58-59 0.3087052046083656 1105 60-61 0.17126876933087232 1105 62-63 0.28981602582815214 1105 64-65 0.366327853406311 1105 66-67 0.4164569920336376 1105 68-69 0.3591868387073518 1105 70-71 0.3617719643152242 1105 72-73 0.42969515993827656 1105 74-75 0.49774649101823343 1105 76-77 0.2961002143091349 1105 78-79 0.38261567704868327 1105 80-81 0.6022210480665855 1105 82-83 0.6637157891236285 1105 84-85 0.5382917423944171 1105 86-87 0.5413775438544732 1105 88-89 0.5613807695241277 1105 90-91 0.7965336521334656 1105 92-93 0.9053803760252102 1105 94-95 0.8866035835668171 1105 96-97 0.9014162590839341 1105 98-99 1.3665239387256847 1105 100-101 1.4106998691174333 1106 1 0.09628668464142365 1106 2 0.12290728489351466 1106 3 0.08856700480702884 1106 4 0.09467166294886908 1106 5 0.10002058982696127 1106 6 -0.013160938426416635 1106 7 0.09666831909576956 1106 8 0.0496505296135723 1106 9 0.10656824293485556 1106 10-11 -0.03331982008975132 1106 12-13 0.057705783846593306 1106 14-15 -0.029868289482855914 1106 16-17 -0.01261583961493784 1106 18-19 0.06775247855510713 1106 20-21 0.09743390716198519 1106 22-23 0.17446017304556705 1106 24-25 0.050031051057743525 1106 26-27 0.07694716058576745 1106 28-29 0.09549190082896075 1106 30-31 0.12452139352600256 1106 32-33 0.15599385834316593 1106 34-35 0.14904755997859098 1106 36-37 0.05197715959833005 1106 38-39 0.13210777971567467 1106 40-41 0.17859969429363787 1106 42-43 0.18197933162695534 1106 44-45 0.11270128683482739 1106 46-47 0.003560988807642218 1106 48-49 0.06405795945726567 1106 50-51 0.11588385331278772 1106 52-53 0.10259341281726364 1106 54-55 0.08137955846655842 1106 56-57 0.07769296330265973 1106 58-59 0.05102799207035247 1106 60-61 0.19707430132765325 1106 62-63 0.23141794597372467 1106 64-65 0.07132696127782623 1106 66-67 0.03174088456960078 1106 68-69 0.1746678497496177 1106 70-71 0.21970928825739122 1106 72-73 0.2654514817032023 1106 74-75 0.34374372248162643 1106 76-77 0.24002471935913405 1106 78-79 0.4000204221718988 1106 80-81 0.43967787798837676 1106 82-83 0.5986579795734883 1106 84-85 0.49854888795590213 1106 86-87 0.5442454837078827 1106 88-89 0.5723053359665684 1106 90-91 0.5651673151729781 1106 92-93 0.5326062490763945 1106 94-95 0.48404890771954 1106 96-97 0.5751663258216446 1106 98-99 1.0176031391034925 1106 100-101 1.1157334367798484 1107 1 0.1410574547876351 1107 2 0.07146316920073303 1107 3 -0.029139822711197638 1107 4 -0.011105503534110994 1107 5 0.04173582129673292 1107 6 0.004894740305644518 1107 7 -0.011801914672538771 1107 8 0.013785778313220476 1107 9 0.05613024128616928 1107 10-11 -0.04422526932490456 1107 12-13 0.03549443616737591 1107 14-15 0.02159985017310362 1107 16-17 0.04265387508063867 1107 18-19 -0.04189545052715715 1107 20-21 0.020033593904372538 1107 22-23 0.13225343883985374 1107 24-25 0.07835315857116143 1107 26-27 0.08858964093460742 1107 28-29 0.04885446026079876 1107 30-31 0.012186533428810264 1107 32-33 -0.01361492173791845 1107 34-35 0.02088427724084596 1107 36-37 0.05940231743924329 1107 38-39 0.15382844390558859 1107 40-41 0.06674109603642364 1107 42-43 0.026125815639751693 1107 44-45 0.039057360782287276 1107 46-47 0.02503940564599816 1107 48-49 -0.024508588467931247 1107 50-51 0.06906786303076728 1107 52-53 -0.009042005367064121 1107 54-55 -0.05033092080719115 1107 56-57 -0.07351914077388244 1107 58-59 -0.04878211803259802 1107 60-61 -0.04346697314589676 1107 62-63 0.023822727397622145 1107 64-65 0.06604207718859101 1107 66-67 -0.023543356806918325 1107 68-69 0.05432123829468338 1107 70-71 0.046691917021249196 1107 72-73 -0.02214733153967785 1107 74-75 0.0422788085082928 1107 76-77 0.010599910724117478 1107 78-79 0.06717008886091946 1107 80-81 0.08587569982487864 1107 82-83 0.19961395636993728 1107 84-85 0.27799631030261196 1107 86-87 0.2934425189948975 1107 88-89 0.2145557463594443 1107 90-91 0.34205152163375985 1107 92-93 0.42563384699896645 1107 94-95 0.3534524745981926 1107 96-97 0.36838296530895676 1107 98-99 0.8255884200692556 1107 100-101 0.8537028060207774 1108 1 0.0992775756888804 1108 2 0.031408609947877864 1108 3 -0.03017857048265782 1108 4 -0.025711995055594628 1108 5 0.007392958216883017 1108 6 -1.2514677647601502E-4 1108 7 -0.07611329602514871 1108 8 0.031893929461602966 1108 9 -0.028693073954720205 1108 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-1.463152120042249 2316 96-97 -1.6162041208372209 2316 98-99 -1.2345705374710434 2316 100-101 -1.600779680595135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144.0 3 91.0 4 295.0 5 767.0 6 1481.0 7 2789.0 8 4868.0 9 7555.0 10 10598.0 11 13164.0 12 16466.0 13 19410.0 14 22689.0 15 25936.0 16 29832.0 17 34731.0 18 40196.0 19 45411.0 20 48934.0 21 52280.0 22 58411.0 23 69679.0 24 86248.0 25 108879.0 26 138102.0 27 174826.0 28 219962.0 29 271709.0 30 333338.0 31 404104.0 32 491953.0 33 609595.0 34 784369.0 35 1081892.0 36 1398980.0 37 1933541.0 38 1778441.0 39 399741.0 40 777.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.963328520675745 31.067778510138716 28.71349965399682 22.25539331518872 2 22.49357966623218 29.300793569668272 28.199683944987324 20.005942819112228 3 21.198563650838302 28.466448626511166 28.549043739596335 21.7859439830542 4 21.596780254295595 28.745126066385012 28.42472208540705 21.23337159391234 5 22.152464050722802 28.682067661799376 28.40341010887678 20.762058178601045 6 21.979148635500852 28.590922215789032 27.938955674669025 21.490973474041088 7 22.17470899756122 28.806720958582883 28.068977886080987 20.94959215777491 8 21.422743724599393 28.766303581434528 28.256258239925746 21.554694454040334 9 21.16152704664666 28.555008640515656 28.735503971634074 21.547960341203606 10-11 21.26182814369451 28.989471747246203 28.19341401647123 21.55528609258806 12-13 21.272508474019844 29.027491041004332 28.030907695670958 21.669092789304866 14-15 21.422160820967072 29.026129378119236 27.881339286846785 21.67037051406691 16-17 21.582294462904358 28.870120425575667 27.844321806684412 21.70326330483556 18-19 21.575156703149283 28.878211752381787 27.824806960969894 21.721824583499032 20-21 21.67298658556876 28.788393297484916 27.883279190135145 21.655340926811178 22-23 21.744707284786372 28.789130731102187 27.78945897777021 21.676703006341235 24-25 21.6181610015273 28.775187032791084 27.798767489906908 21.80788447577471 26-27 21.81885349348994 28.663693326314853 27.862004079024288 21.65544910117092 28-29 21.826090553475932 28.718022065951438 27.793293399423398 21.662593981149232 30-31 21.778374132923027 28.732645519655797 27.74559101508991 21.74338933233126 32-33 21.81234666992098 28.765612335674014 27.6632702305937 21.7587707638113 34-35 21.83859574772289 28.78857905554891 27.646534973753482 21.726290222974708 36-37 21.855177558479795 28.805387654063765 27.609073689416675 21.730361098039772 38-39 21.880083407343403 28.776993078067193 27.61504941556335 21.72787409902605 40-41 21.870008743806473 28.821577382687263 27.609774409793065 21.698639463713203 42-43 21.8524266616353 28.837890423258468 27.57864144739649 21.731041467709744 44-45 21.86968927828955 28.864057975110047 27.574601978144525 21.69165076845588 46-47 21.8873999724287 28.875385751011024 27.52757269784785 21.70964157871242 48-49 21.911704750897723 28.853227306182404 27.507977359790747 21.727090583129122 50-51 21.937428189721793 28.851133017363832 27.487233818999368 21.724204973915004 52-53 21.859568347471146 28.939990634904937 27.45620605447799 21.744234963145924 54-55 21.902571925047663 28.915837391394657 27.398269123230705 21.783321560326968 56-57 21.9259456221891 28.93696884209372 27.386492787696138 21.75059274802105 58-59 21.89305975614351 29.0309245140199 27.353748968013935 21.722266761822652 60-61 21.921578663451402 28.993850506575896 27.32744560250039 21.75712522747231 62-63 21.879199826154593 29.0256543218397 27.355679867041836 21.739465984963864 64-65 21.896022451212534 29.089603630111977 27.309932523904962 21.704441394770527 66-67 21.88753634207253 29.120575239293657 27.281323385385125 21.710565033248688 68-69 21.884585241385217 29.148399903860206 27.278458539993938 21.68855631476064 70-71 21.91543050347993 29.222630526535358 27.20936554061134 21.652573429373373 72-73 21.879165372936207 29.21373801377577 27.207265832765863 21.699830780522163 74-75 21.94279696723049 29.25090391681587 27.17024675271259 21.63605236324105 76-77 21.901644778152185 29.27802760365149 27.20345184843141 21.616875769764913 78-79 21.87671901070788 29.268248210923137 27.183270674062047 21.671762104306936 80-81 21.84371443838891 29.32833843888657 27.16089037565559 21.66705674706893 82-83 21.945956427105877 29.37875241059431 27.086019219840885 21.589271942458925 84-85 21.845646127932937 29.392893982380446 27.114831125759874 21.64662876392674 86-87 21.845639762193265 29.437350400821337 27.087090411625308 21.629919425360082 88-89 21.82935782118186 29.492742671335435 27.08129326348983 21.596606243992873 90-91 21.827318114970073 29.494999029338416 27.059946501927314 21.6177363537642 92-93 21.816176941717895 29.534046649149477 27.04313517233056 21.60664123680207 94-95 21.794254568957435 29.574224602770823 27.026632413378554 21.604888414893185 96-97 21.800348691720547 29.608377704005807 27.008172902296884 21.583100701976768 98-99 21.821966075539105 29.63605352329169 26.9436647877559 21.59831561341331 100-101 21.87938344512673 29.603526714766424 26.90426182784066 21.612828012266185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 159904.0 1 91582.0 2 17030.0 3 10111.5 4 8903.5 5 7583.0 6 6696.5 7 6706.5 8 6659.0 9 5997.0 10 5598.0 11 6385.0 12 10665.5 13 10502.0 14 6777.0 15 7418.0 16 8468.0 17 10791.0 18 15473.5 19 18068.0 20 20795.5 21 24979.5 22 30668.5 23 39635.5 24 50110.0 25 62104.5 26 76873.0 27 93517.0 28 107436.5 29 122722.0 30 141692.0 31 162131.5 32 181580.0 33 199281.5 34 218958.5 35 239579.5 36 262772.5 37 286485.0 38 304754.0 39 315513.0 40 328840.0 41 344535.5 42 354191.0 43 363865.5 44 372173.0 45 380004.0 46 387731.5 47 392165.5 48 400022.5 49 407026.5 50 405160.0 51 396234.5 52 384959.0 53 365872.0 54 335870.0 55 311000.0 56 284022.0 57 250083.0 58 224396.0 59 195922.5 60 160208.5 61 125511.5 62 91590.0 63 63366.0 64 42737.5 65 27469.0 66 17574.0 67 11962.5 68 7296.5 69 3756.5 70 1744.5 71 758.5 72 378.0 73 207.0 74 158.5 75 130.5 76 81.5 77 58.5 78 42.0 79 31.0 80 24.5 81 21.0 82 13.0 83 7.5 84 8.5 85 8.0 86 4.0 87 2.0 88 2.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15929590464032328 2 0.0 3 0.0 4 8.860135211259198E-4 5 5.875668613782416E-4 6 7.461166493691957E-5 7 2.1450853669364376E-4 8 0.0 9 2.1450853669364376E-4 10-11 3.217628050404656E-4 12-13 0.0 14-15 0.0 16-17 1.3989687175672418E-5 18-19 0.0 20-21 0.0 22-23 6.01556548553914E-4 24-25 0.0 26-27 1.1191749740537934E-4 28-29 0.005152868109706008 30-31 0.0035906863750892544 32-33 1.2590718458105178E-4 34-35 8.720238339502475E-4 36-37 3.777215537431553E-4 38-39 9.932677894727417E-4 40-41 0.0028818755581885183 42-43 0.0011051852868781211 44-45 6.10883006671029E-4 46-47 0.008300547724232303 48-49 0.008440444595989026 50-51 0.007372565141579365 52-53 0.014213522170483179 54-55 0.005815046636021169 56-57 8.393812305403452E-4 58-59 0.0013057041363960923 60-61 0.0 62-63 1.3057041363960926E-4 64-65 8.440444595989026E-4 66-67 9.979310185312992E-4 68-69 0.002769958060783139 70-71 0.001841975478130202 72-73 0.005642507160854542 74-75 0.0021917176575220122 76-77 0.0014968965277969489 78-79 0.0016228037123780007 80-81 3.310892631575806E-4 82-83 0.002266329322458932 84-85 3.730583246845978E-5 86-87 0.001865291623422989 88-89 2.098453076350863E-4 90-91 0.0010772059125267763 92-93 0.0013523364269816672 94-95 9.233193535943796E-4 96-97 9.839413313556266E-4 98-99 0.522155747373856 100-101 2.2383499481075868E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0722184E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.90734206162386 #Duplication Level Percentage of deduplicated Percentage of total 1 88.5732948861241 64.57643507787728 2 6.914287318616719 10.082046213014742 3 1.8779026802577667 4.107386792039797 4 0.8467820406956699 2.469465115705564 5 0.4652468093522844 1.695995413626305 6 0.2685152043162893 1.1746037909900715 7 0.17430158934546713 0.8895505917406263 8 0.11850216091923704 0.6911742064944328 9 0.08681435293042743 0.5696463352461489 >10 0.6683154519968993 8.768271030454816 >50 0.00496519882379978 0.20882784751836816 >100 8.08072445807163E-4 0.11236454048626002 >500 6.605857655573684E-5 0.02920997861910781 >1k 3.302926649448594E-5 0.07375936880986611 >5k 6.605853298897188E-5 0.2779642161842261 >10k+ 9.908779948345782E-5 4.27329948119243 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143061 1.3342524246925813 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23601 0.22011373802202985 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10795 0.10067911537425583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.326458117114946E-6 2 0.0 9.326458117114946E-6 0.0 9.326458117114946E-6 9.326458117114946E-6 3 0.0 9.326458117114946E-6 0.0 9.326458117114946E-6 1.865291623422989E-5 4 0.0 2.797937435134484E-5 0.0 1.865291623422989E-5 2.797937435134484E-5 5 0.0 3.730583246845978E-5 0.0 1.865291623422989E-5 5.595874870268968E-5 6 0.0 3.730583246845978E-5 0.0 2.797937435134484E-5 7.461166493691957E-5 7 0.0 5.595874870268968E-5 0.0 2.797937435134484E-5 8.393812305403451E-5 8 0.0 5.595874870268968E-5 0.0 2.797937435134484E-5 9.326458117114946E-5 9 0.0 5.595874870268968E-5 0.0 3.730583246845978E-5 1.3057041363960924E-4 10-11 9.326458117114946E-6 6.528520681980462E-5 0.0 4.196906152701726E-5 1.5388655893239662E-4 12-13 9.326458117114946E-6 8.393812305403451E-5 0.0 6.0621977761247145E-5 1.911923914008564E-4 14-15 9.326458117114946E-6 8.860135211259199E-5 0.0 6.528520681980462E-5 2.238349948107587E-4 16-17 9.326458117114946E-6 9.792781022970693E-5 0.0 7.461166493691957E-5 2.424879110449886E-4 18-19 1.3989687175672418E-5 1.1191749740537936E-4 0.0 8.393812305403451E-5 2.56477598220661E-4 20-21 1.865291623422989E-5 1.212439555224943E-4 0.0 9.326458117114946E-5 2.9844665974767826E-4 22-23 2.797937435134484E-5 1.212439555224943E-4 0.0 9.326458117114946E-5 3.4507895033325303E-4 24-25 2.797937435134484E-5 1.3989687175672418E-4 0.0 9.326458117114946E-5 3.5440540845036795E-4 26-27 2.797937435134484E-5 1.4456010081528164E-4 0.0 9.326458117114946E-5 3.6373186656748287E-4 28-29 4.663229058557473E-5 1.4922332987383913E-4 0.0 9.326458117114946E-5 3.963744699773852E-4 30-31 5.595874870268968E-5 1.6787624610806903E-4 0.0 9.326458117114946E-5 4.1036415715305763E-4 32-33 5.595874870268968E-5 1.6787624610806903E-4 0.0 9.326458117114946E-5 4.4300676056295996E-4 34-35 6.0621977761247145E-5 1.6787624610806903E-4 0.0 1.0259103928826441E-4 4.8031259303141975E-4 36-37 6.994843587836209E-5 1.8652916234229892E-4 0.0 1.0259103928826441E-4 5.036287383242071E-4 38-39 7.461166493691957E-5 2.1450853669364376E-4 0.0 1.0259103928826441E-4 5.22281654558437E-4 40-41 7.461166493691957E-5 2.1450853669364376E-4 0.0 1.0259103928826441E-4 5.22281654558437E-4 42-43 8.393812305403451E-5 2.238349948107587E-4 0.0 1.0259103928826441E-4 5.549242579683392E-4 44-45 1.0725426834682188E-4 2.3782468198643112E-4 0.0 1.0725426834682188E-4 6.015565485539139E-4 46-47 1.2590718458105178E-4 2.5181436916210355E-4 0.0 1.1191749740537936E-4 6.388623810223738E-4 48-49 1.4456010081528164E-4 2.5181436916210355E-4 0.0 1.1191749740537936E-4 6.575152972566037E-4 50-51 1.6787624610806903E-4 2.5181436916210355E-4 0.0 1.1658072646393683E-4 6.715049844322761E-4 52-53 2.0518207857652882E-4 2.6580405633777593E-4 0.0 1.212439555224943E-4 6.80831442549391E-4 54-55 2.3782468198643112E-4 2.9844665974767826E-4 0.0 1.212439555224943E-4 6.99484358783621E-4 56-57 2.7979374351344837E-4 2.9844665974767826E-4 0.0 1.212439555224943E-4 7.181372750178508E-4 58-59 3.217628050404657E-4 3.217628050404657E-4 0.0 1.212439555224943E-4 7.507798784277531E-4 60-61 3.823847828017128E-4 3.2642603409902313E-4 0.0 1.3057041363960924E-4 8.020753980718853E-4 62-63 4.476699896215174E-4 3.2642603409902313E-4 0.0 1.3057041363960924E-4 8.440444595989026E-4 64-65 5.176184254998795E-4 3.404157212746955E-4 0.0 1.3057041363960924E-4 8.720238339502474E-4 66-67 5.549242579683392E-4 3.4507895033325303E-4 0.0 1.3057041363960924E-4 8.766870630088049E-4 68-69 6.575152972566037E-4 3.4507895033325303E-4 0.0 1.3057041363960924E-4 9.046664373601498E-4 70-71 7.461166493691957E-4 3.5440540845036795E-4 0.0 1.3057041363960924E-4 9.606251860628395E-4 72-73 8.4870768865746E-4 3.6373186656748287E-4 0.0 1.3523364269816672E-4 9.979310185312992E-4 74-75 9.792781022970693E-4 3.7305832468459784E-4 0.0 1.4922332987383913E-4 0.0010072574766484142 76-77 0.001123838203112351 4.0103769903594266E-4 0.0 1.4922332987383913E-4 0.0010305736219412016 78-79 0.001221766013342058 4.1036415715305763E-4 0.0 1.4922332987383913E-4 0.0010585529962925462 80-81 0.0013756525722744546 4.1036415715305763E-4 0.0 1.4922332987383913E-4 0.0010911955997024488 82-83 0.0015295391312068513 4.1036415715305763E-4 0.0 1.6321301704951154E-4 0.0011611440355808107 84-85 0.001911923914008564 4.2435384432873E-4 0.0 1.7720270422518397E-4 0.0012077763261663854 86-87 0.0024808378591525755 4.33680302445845E-4 0.0 1.8186593328374143E-4 0.0012357557005177305 88-89 0.0034741056486253174 4.4300676056295996E-4 0.0 1.911923914008564E-4 0.0012683983039276326 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 405 0.0 32.82396 3 CGCGTAG 230 0.0 28.900034 54-55 TCGCGTA 265 0.0 24.189367 52-53 CAACGCA 7915 0.0 19.793964 12-13 ACGTACG 365 0.0 19.510672 20-21 CGTACGC 390 0.0 19.47732 20-21 ATCAACG 8290 0.0 19.041796 10-11 CTCGCGT 355 0.0 18.725624 52-53 TCAACGC 8005 0.0 17.52536 10-11 ACTCGCG 385 0.0 17.266726 50-51 AACGCAG 8490 0.0 16.636005 12-13 AGTGGTA 9885 0.0 16.425829 5 GGTATCA 10675 0.0 15.610292 8 GTATCAA 10900 0.0 15.244506 9 AAGCAGT 11355 0.0 15.118876 1 TGGTATC 11400 0.0 14.7425375 7 ACGCAGA 10985 0.0 14.197278 14-15 CGCTAGT 475 0.0 13.993766 90-91 ATCGGAA 1780 0.0 13.603108 2 TATCAAC 11775 0.0 13.305305 9 >>END_MODULE