FastQCFastQC Report
Sat 18 Jun 2016
SRR578578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9672123
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114517011.839903193952352No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT252460.26101818597633636No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG108510.11218839958921117No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA97580.100887881595385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2579300.091.6329965
AAGCAGT2599000.091.290221
GTATCAA2596450.091.212649
TGGTATC2597100.091.11217
GGTATCA2599200.091.075488
GTGGTAT2617800.090.468626
AGCAGTG2623300.090.4401552
GCAGTGG2621650.090.307273
CAGTGGT2624650.090.08864
TCAACGC2590300.046.27742412-13
CAACGCA2586250.046.1966312-13
AACGCAG2593700.046.19634214-15
ATCAACG2586350.046.178810-11
CGCAGAG2603850.045.99978316-17
ACGCAGA2599800.045.95649714-15
TATCAAC2606050.045.9089610-11
CAGAGTA2597650.045.69289818-19
AGAGTAC2557050.045.59155318-19
GCAGAGT2621650.045.4358316-17
GAGTACT1440000.045.4080220-21
Produced by FastQC (version 0.11.5)