FastQCFastQC Report
Sat 18 Jun 2016
SRR578578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9672123
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200120.20690390310379633No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103140.10663636101401937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3950.030.012733
CGCGTAG2450.024.18718154-55
CTCGCGT2800.021.16378452-53
TCGCGTA2900.020.43399852-53
CAACGCA73100.018.90438512-13
ATCAACG75150.018.45177810-11
ACGTACG3250.018.2359620-21
TCAACGC73400.017.8260210-11
ACTCGCG3250.017.50407850-51
CGTACGC3300.017.2412720-21
AACGCAG76700.017.02815212-13
ATCGGAA17850.017.0036662
AGTGGTA91050.015.8312724
CGTAGCG4000.015.40723456-57
AAGCAGT102600.014.37616351
GGTATCA100750.014.3067757
GTATCAA101400.014.1215438
ACGCAGA100600.013.90161914-15
TGGTATC105450.013.8040016
TATCAAC107850.013.3201389
Produced by FastQC (version 0.11.5)