FastQCFastQC Report
Sat 18 Jun 2016
SRR578577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9973644
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119219611.953464551171066No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT287520.28827979021509087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGCCAGCTTTCCAAGAA126030.12636304243464075No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG104900.10517720504160766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2590650.091.3151255
GTATCAA2600150.091.070139
AAGCAGT2615600.090.8897251
GGTATCA2606700.090.88558
TGGTATC2608850.090.7290957
GTGGTAT2616000.090.5769356
GCAGTGG2620900.090.495333
AGCAGTG2632700.090.2587052
CAGTGGT2621050.090.128444
TCAACGC2588900.046.27295712-13
ATCAACG2587300.046.17487310-11
CAACGCA2591200.046.15120712-13
AACGCAG2603550.046.1221614-15
CGCAGAG2611300.046.0092616-17
ACGCAGA2603800.045.97358314-15
TATCAAC2606000.045.84808710-11
CAGAGTA2599700.045.66661518-19
AGAGTAC2549050.045.63951518-19
GAGTACT1520100.045.447220-21
GCAGAGT2629400.045.3131616-17
Produced by FastQC (version 0.11.5)