##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9973644 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.637278812037003 31.0 31.0 34.0 27.0 34.0 2 30.942413625350977 31.0 31.0 34.0 27.0 34.0 3 31.008726198769477 31.0 31.0 34.0 27.0 34.0 4 34.418830068528614 37.0 35.0 37.0 30.0 37.0 5 34.5285552602439 37.0 35.0 37.0 30.0 37.0 6 34.49616338822601 37.0 35.0 37.0 30.0 37.0 7 34.491096534025075 37.0 35.0 37.0 30.0 37.0 8 34.43286836787036 37.0 35.0 37.0 30.0 37.0 9 35.82215236477259 39.0 35.0 39.0 30.0 39.0 10-11 35.80717438881917 39.0 35.0 39.0 30.0 39.0 12-13 35.84584440752046 39.0 35.0 39.0 30.0 39.0 14-15 36.89811000873903 40.0 36.0 41.0 30.5 41.0 16-17 36.85626848120907 40.0 36.0 41.0 30.0 41.0 18-19 36.86173754547485 40.0 36.0 41.0 30.5 41.0 20-21 36.749543346443886 39.5 36.0 41.0 30.0 41.0 22-23 36.45106116681125 39.0 36.0 40.5 29.5 41.0 24-25 36.35553835689343 39.0 36.0 40.0 29.5 41.0 26-27 36.24443257649862 39.0 36.0 40.5 29.0 41.0 28-29 35.957790302120266 39.0 35.0 41.0 27.0 41.0 30-31 35.71732543291098 39.0 34.5 40.5 26.5 41.0 32-33 35.34141057170278 38.0 34.0 40.0 25.0 41.0 34-35 35.12239232721761 38.0 34.0 40.0 25.0 41.0 36-37 34.798687169905 38.0 33.5 40.0 25.0 41.0 38-39 34.13486665455474 37.0 33.0 40.0 24.0 41.0 40-41 34.314451067232795 37.0 33.0 40.0 24.0 41.0 42-43 34.24620554934586 37.0 33.0 40.0 24.0 41.0 44-45 34.11079776859892 37.0 33.0 40.0 23.0 41.0 46-47 33.9060955053138 37.0 33.0 40.0 23.0 41.0 48-49 33.57367883794529 37.0 33.0 40.0 21.5 41.0 50-51 32.38312822274386 36.0 31.5 39.0 16.5 40.5 52-53 32.02912877179093 36.5 31.0 39.0 10.0 40.0 54-55 31.6828430511456 37.0 31.0 40.0 2.0 41.0 56-57 31.231711548958437 37.0 30.0 40.0 2.0 41.0 58-59 30.913935718980945 36.5 29.5 40.0 2.0 41.0 60-61 30.623945370418276 36.0 29.0 40.0 2.0 41.0 62-63 30.30047006891363 35.5 28.0 39.0 2.0 41.0 64-65 29.96423589011198 35.0 28.0 39.0 2.0 41.0 66-67 29.649345865964335 35.0 28.0 39.0 2.0 40.5 68-69 29.323925287487704 35.0 28.0 38.5 2.0 40.0 70-71 28.944422169068798 35.0 26.5 38.0 2.0 40.0 72-73 28.54488204110754 35.0 26.0 37.0 2.0 39.5 74-75 28.17106360523797 34.0 26.0 36.5 2.0 39.0 76-77 27.77696722481773 34.0 26.0 36.0 2.0 39.0 78-79 27.41013826039911 34.0 25.0 36.0 2.0 38.5 80-81 27.03213489472855 34.0 24.5 35.0 2.0 37.0 82-83 26.63918353211725 34.0 23.5 35.0 2.0 37.0 84-85 26.281028629054738 33.0 22.5 35.0 2.0 36.5 86-87 25.932929428802552 33.0 20.5 35.0 2.0 36.0 88-89 25.554408298511557 33.0 19.5 35.0 2.0 36.0 90-91 25.18840566196267 33.0 18.0 35.0 2.0 35.0 92-93 24.835058129205336 32.0 12.5 35.0 2.0 35.0 94-95 24.379342745740672 32.0 4.5 35.0 2.0 35.0 96-97 23.87387924614113 32.0 2.0 35.0 2.0 35.0 98-99 23.325161445505778 32.0 2.0 35.0 2.0 35.0 100-101 21.969816197570317 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5478315682237529 1101 2 0.5861661910048248 1101 3 0.6032372828990162 1101 4 0.6412878583248443 1101 5 0.6421754579018994 1101 6 0.5060498504005011 1101 7 0.4450077039000462 1101 8 0.4788338310984912 1101 9 0.692778860589911 1101 10-11 0.7484058456321705 1101 12-13 0.7498638138886307 1101 14-15 0.7656132192511009 1101 16-17 0.8125642685895045 1101 18-19 0.8516547050262773 1101 20-21 0.841614757700448 1101 22-23 0.7834750889800262 1101 24-25 0.8333233225467183 1101 26-27 1.0572731121408836 1101 28-29 1.0962797126457104 1101 30-31 1.1247171007845083 1101 32-33 1.1369670463825088 1101 34-35 1.2003101314911362 1101 36-37 1.1858355646931642 1101 38-39 1.024724500135349 1101 40-41 1.0895401862964604 1101 42-43 1.345446696306368 1101 44-45 1.2759389770532934 1101 46-47 1.3377466361284078 1101 48-49 1.2145817490875501 1101 50-51 1.2259794895258622 1101 52-53 1.1939346688940446 1101 54-55 1.0672883914024816 1101 56-57 1.0245859334812195 1101 58-59 1.0366403861608653 1101 60-61 1.1423501070803503 1101 62-63 1.1997405601470348 1101 64-65 1.0246623561789292 1101 66-67 1.0666051313294176 1101 68-69 1.0650840545210194 1101 70-71 1.1652499948371293 1101 72-73 1.1880445453084008 1101 74-75 1.222477537803485 1101 76-77 1.2438873709416747 1101 78-79 1.1319759744271956 1101 80-81 1.182289930307327 1101 82-83 1.214065232911274 1101 84-85 1.1690015423322038 1101 86-87 1.1851764580216724 1101 88-89 1.3334565859353873 1101 90-91 1.3268221780322307 1101 92-93 1.441742444127346 1101 94-95 1.563735249396224 1101 96-97 1.6569456127108602 1101 98-99 1.7820372093042351 1101 100-101 1.9901252498803998 1102 1 0.5369796914162777 1102 2 0.48033493931374593 1102 3 0.5104648229808291 1102 4 0.662675816264219 1102 5 0.5772120141731492 1102 6 0.4595769402917398 1102 7 0.37555918243066344 1102 8 0.5774518645617519 1102 9 0.6106408592050414 1102 10-11 0.7171413307484258 1102 12-13 0.6686240409514639 1102 14-15 0.7476058875542719 1102 16-17 0.7455285437569046 1102 18-19 0.7418391786184202 1102 20-21 0.8750294583887595 1102 22-23 0.750916657241504 1102 24-25 0.7327761963978148 1102 26-27 0.9645168275639548 1102 28-29 0.8057910562890385 1102 30-31 1.028483057592993 1102 32-33 0.9775965396583643 1102 34-35 0.9784692218260886 1102 36-37 1.0542345143542633 1102 38-39 0.9437115133143124 1102 40-41 1.0228544954471346 1102 42-43 1.1895928955268857 1102 44-45 1.1599498419828294 1102 46-47 1.2131006311123826 1102 48-49 1.0378616275534114 1102 50-51 1.1322841214648562 1102 52-53 1.0512786212301464 1102 54-55 0.9106388361249635 1102 56-57 1.0300096227996391 1102 58-59 0.7761288541968021 1102 60-61 1.0580525620895038 1102 62-63 1.0976400088440172 1102 64-65 0.9683741566195181 1102 66-67 1.1160043122848364 1102 68-69 1.0891834856189035 1102 70-71 0.9789998261055324 1102 72-73 1.0543290133943017 1102 74-75 1.1438914044024422 1102 76-77 1.0796283567985014 1102 78-79 0.9031131593539392 1102 80-81 0.9586802355286892 1102 82-83 1.0292278712988008 1102 84-85 0.9742176912788452 1102 86-87 1.0587519687116327 1102 88-89 1.165007786904777 1102 90-91 1.2679661857199243 1102 92-93 1.4426113340603628 1102 94-95 1.4735719995386276 1102 96-97 1.6024737302734415 1102 98-99 1.7721305909248954 1102 100-101 2.024286536447164 1103 1 0.3548903534394938 1103 2 0.4793959857001262 1103 3 0.48238651854793346 1103 4 0.49291167263124436 1103 5 0.4913094135524787 1103 6 0.38204598361549813 1103 7 0.3241832013187107 1103 8 0.4116826781278604 1103 9 0.4895115685402729 1103 10-11 0.5581937397922374 1103 12-13 0.5211817827136898 1103 14-15 0.6471729058578077 1103 16-17 0.6062121951878723 1103 18-19 0.5706949930908678 1103 20-21 0.6963408510851892 1103 22-23 0.4904901733964806 1103 24-25 0.5105102281591272 1103 26-27 0.7249070080522628 1103 28-29 0.6873691720942929 1103 30-31 0.7582299192302244 1103 32-33 0.8122242482857587 1103 34-35 0.8205660439870428 1103 36-37 0.8524532819173203 1103 38-39 0.8895208335834468 1103 40-41 0.8339015060269972 1103 42-43 0.9536534102962975 1103 44-45 0.8869784960275311 1103 46-47 0.8174670091026144 1103 48-49 1.0152747875771482 1103 50-51 0.9080272361961832 1103 52-53 0.8654008856623605 1103 54-55 0.8278245425855921 1103 56-57 0.810021642756162 1103 58-59 0.7758363699863651 1103 60-61 0.7952347656507257 1103 62-63 0.8959595119799957 1103 64-65 0.9350074218676632 1103 66-67 0.926789574164669 1103 68-69 0.9484852275548299 1103 70-71 0.9078151056580559 1103 72-73 0.867649947391886 1103 74-75 0.8131394161719605 1103 76-77 0.8137111675216282 1103 78-79 0.8625294071704168 1103 80-81 0.8985134689936665 1103 82-83 0.9509944971026343 1103 84-85 0.9709967446155332 1103 86-87 1.003030146223601 1103 88-89 1.059825921668871 1103 90-91 1.1499170526071296 1103 92-93 1.1637984334513227 1103 94-95 1.2450230138464242 1103 96-97 1.2564741324766864 1103 98-99 1.3917958825648924 1103 100-101 1.6886480015495735 1104 1 0.3719135737782686 1104 2 0.2554140539141905 1104 3 0.36887831417288197 1104 4 0.5323916113369478 1104 5 0.4413651688640243 1104 6 0.39039824892845587 1104 7 0.40048839226786015 1104 8 0.3604461224173292 1104 9 0.4512579927832192 1104 10-11 0.5190829304480857 1104 12-13 0.49429978223481186 1104 14-15 0.45604558870199696 1104 16-17 0.5885875028365462 1104 18-19 0.5124876962044027 1104 20-21 0.5657490901837221 1104 22-23 0.4681534397444551 1104 24-25 0.5374635914358521 1104 26-27 0.5326121397414383 1104 28-29 0.4998064459261542 1104 30-31 0.5569423862456091 1104 32-33 0.5816283556514179 1104 34-35 0.6231183488784637 1104 36-37 0.6447141095965137 1104 38-39 0.7048138455767017 1104 40-41 0.7297193950531167 1104 42-43 0.7197478178535093 1104 44-45 0.7626636029309282 1104 46-47 0.7296850693071164 1104 48-49 0.7844337512292867 1104 50-51 0.6258239513608359 1104 52-53 0.6352083611443291 1104 54-55 0.7275141086443817 1104 56-57 0.669216634426526 1104 58-59 0.6638747667108937 1104 60-61 0.604433611104529 1104 62-63 0.7094159850589463 1104 64-65 0.7813941858806217 1104 66-67 0.6536376311499623 1104 68-69 0.6611820850065051 1104 70-71 0.7687791685841141 1104 72-73 0.750812783908831 1104 74-75 0.6992050883733576 1104 76-77 0.7913188920693877 1104 78-79 0.7328153750207669 1104 80-81 0.6705767061655976 1104 82-83 0.6251344388182716 1104 84-85 0.6941189999369328 1104 86-87 0.6836584762310309 1104 88-89 0.8007363305426907 1104 90-91 0.8108380987695902 1104 92-93 0.9029091004545826 1104 94-95 0.9800046505250712 1104 96-97 1.0652636180320876 1104 98-99 1.1636265366451362 1104 100-101 1.3673405050626997 1105 1 0.05611014576686202 1105 2 0.03286751093756379 1105 3 0.16007574767533228 1105 4 0.12864927415186145 1105 5 0.24749396135299406 1105 6 0.22908851346804937 1105 7 0.23399247310746318 1105 8 0.29414467777476005 1105 9 0.1989976245435372 1105 10-11 0.1641311311460285 1105 12-13 0.13815122092167798 1105 14-15 -0.04144730130255425 1105 16-17 0.05317755476914243 1105 18-19 -0.04004146760235017 1105 20-21 0.26455610945781416 1105 22-23 0.24764884603580128 1105 24-25 0.3015208322799836 1105 26-27 0.5108766787460155 1105 28-29 0.47380130219899286 1105 30-31 0.4766389089964207 1105 32-33 0.564811163614614 1105 34-35 0.5300552823490747 1105 36-37 0.5006566166667028 1105 38-39 0.6927581613247753 1105 40-41 0.5970547926880485 1105 42-43 0.608097302360413 1105 44-45 0.636231524099756 1105 46-47 0.6563872336174441 1105 48-49 0.7392496601352505 1105 50-51 0.6528467804461542 1105 52-53 0.5549466770468925 1105 54-55 0.7006274758485951 1105 56-57 0.6772430755605399 1105 58-59 0.7227689464506355 1105 60-61 0.6483078701790213 1105 62-63 0.7001839736570084 1105 64-65 0.7304042373178952 1105 66-67 0.8485206272781625 1105 68-69 0.8053212109443812 1105 70-71 0.595914842864655 1105 72-73 0.7562743822676516 1105 74-75 0.6624792088449531 1105 76-77 0.5273261652571399 1105 78-79 0.6390483113265297 1105 80-81 0.6607461571259421 1105 82-83 0.6653199178577154 1105 84-85 0.6493687247065445 1105 86-87 0.7406456499348693 1105 88-89 0.7895472276610711 1105 90-91 0.8238578633186187 1105 92-93 0.8854391760449047 1105 94-95 0.9677165241142731 1105 96-97 0.9687385564406235 1105 98-99 1.1474213526137476 1105 100-101 1.425607013578773 1106 1 0.08557433786081248 1106 2 0.2008187601595175 1106 3 0.190530928951393 1106 4 0.3391571356572953 1106 5 0.2768193138249302 1106 6 0.25686510140439367 1106 7 0.29685283703533827 1106 8 0.22078868539866647 1106 9 0.1531523277264384 1106 10-11 0.19029561408184037 1106 12-13 0.20756309345694746 1106 14-15 0.17254781811661957 1106 16-17 0.252260930676492 1106 18-19 0.13066524987054606 1106 20-21 0.18612667040111575 1106 22-23 0.22734126458929893 1106 24-25 0.22092290721470675 1106 26-27 0.11905698731651881 1106 28-29 0.17282630151112244 1106 30-31 0.3731764332487302 1106 32-33 0.37307323660097325 1106 34-35 0.3681135503712696 1106 36-37 0.3926996636423894 1106 38-39 0.5696545640622972 1106 40-41 0.27059286798567683 1106 42-43 0.2335282108712704 1106 44-45 0.3155002289647939 1106 46-47 0.39509560125167553 1106 48-49 0.3721694306148393 1106 50-51 0.2920832331718941 1106 52-53 0.31860185505089333 1106 54-55 0.08620081581529249 1106 56-57 0.09455047183797305 1106 58-59 0.38911524831191713 1106 60-61 0.2644330474468042 1106 62-63 0.20173918936410473 1106 64-65 0.3279749157580447 1106 66-67 0.4051151374985267 1106 68-69 0.41228514833790086 1106 70-71 0.32650851052855856 1106 72-73 0.3834035105526361 1106 74-75 0.4534920576451107 1106 76-77 0.4316429099699697 1106 78-79 0.4392092931539331 1106 80-81 0.40683849477021994 1106 82-83 0.3471925163301073 1106 84-85 0.43577577169115145 1106 86-87 0.5537064835526522 1106 88-89 0.4849520972358583 1106 90-91 0.5105966715585666 1106 92-93 0.4923963462152834 1106 94-95 0.4381291276583674 1106 96-97 0.5814535180273381 1106 98-99 0.5829513377332916 1106 100-101 0.8353801480003611 1107 1 0.14146729667652735 1107 2 0.13610322206637449 1107 3 0.21607919803228626 1107 4 0.42922327925916903 1107 5 0.09366372291381708 1107 6 0.2743768017149222 1107 7 0.2968827187602443 1107 8 0.14673934824801194 1107 9 0.0625382178989824 1107 10-11 0.18677320480720283 1107 12-13 0.1832390665310797 1107 14-15 0.3141140041182453 1107 16-17 0.27138493175869627 1107 18-19 0.30711673446732846 1107 20-21 0.18938276889775096 1107 22-23 0.21488553194189564 1107 24-25 0.4598307301695215 1107 26-27 0.19058596249450233 1107 28-29 0.15434273847884583 1107 30-31 0.39226489930672415 1107 32-33 0.5387305537874028 1107 34-35 0.31820884884528056 1107 36-37 0.4099177961961047 1107 38-39 0.4281731775502635 1107 40-41 0.22466526252135566 1107 42-43 0.241366409407064 1107 44-45 0.4478381105120235 1107 46-47 0.3628887551062192 1107 48-49 0.5172208756385217 1107 50-51 0.2842084900941586 1107 52-53 0.3729890172157795 1107 54-55 0.29161960415173027 1107 56-57 0.21970482709937755 1107 58-59 0.4399371976502806 1107 60-61 0.4114294293352465 1107 62-63 0.34980343911071543 1107 64-65 0.5008006164643 1107 66-67 0.5774079137934365 1107 68-69 0.463709375955883 1107 70-71 0.37210226847851047 1107 72-73 0.367685117543342 1107 74-75 0.5051664437573749 1107 76-77 0.5296316477901364 1107 78-79 0.3829542203273242 1107 80-81 0.2646592286492293 1107 82-83 0.25104776360138104 1107 84-85 0.3036094072067925 1107 86-87 0.50433187978836 1107 88-89 0.49896319385478094 1107 90-91 0.4767868122045762 1107 92-93 0.5828370208961751 1107 94-95 0.510386671784385 1107 96-97 0.6356963191563842 1107 98-99 0.6465911337299488 1107 100-101 0.7305847368641736 1108 1 0.15726092941495295 1108 2 0.0919583307481524 1108 3 0.14945238652721216 1108 4 0.3662200614591242 1108 5 0.15566093155621985 1108 6 0.3084776356927037 1108 7 0.27190631673867216 1108 8 0.1856672054977011 1108 9 0.22477917206710174 1108 10-11 0.25070462172912045 1108 12-13 0.06029183927783777 1108 14-15 0.2557901886810967 1108 16-17 0.15527862243943247 1108 18-19 0.1335677475419601 1108 20-21 0.18270213945792335 1108 22-23 0.13485134292859868 1108 24-25 0.36925602400632584 1108 26-27 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2315 40-41 -2.7368708961287034 2315 42-43 -2.2358824623755282 2315 44-45 -1.9187189960584874 2315 46-47 -1.5733267826654398 2315 48-49 -2.0370578737999523 2315 50-51 -1.7469139605367054 2315 52-53 -1.6003312353571282 2315 54-55 -1.6041710418851274 2315 56-57 -1.6154234257976192 2315 58-59 -1.7569037427055036 2315 60-61 -1.5002792743294187 2315 62-63 -1.35881582221203 2315 64-65 -1.3636151325811774 2315 66-67 -1.4344484011711813 2315 68-69 -1.7244307198464561 2315 70-71 -1.4060049900234723 2315 72-73 -1.3239036875551555 2315 74-75 -1.4217794451180055 2315 76-77 -1.346017618829915 2315 78-79 -1.4864271071732453 2315 80-81 -1.530068388018936 2315 82-83 -1.5024985859249682 2315 84-85 -1.5772435654800177 2315 86-87 -1.7031813922826657 2315 88-89 -1.7377067600935767 2315 90-91 -1.7530245246879979 2315 92-93 -1.7977090305456436 2315 94-95 -1.8077691635503292 2315 96-97 -1.9042451807088305 2315 98-99 -1.8433274761379117 2315 100-101 -2.0629801246679804 2316 1 -1.009390243592911 2316 2 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-2.129824355705029 2316 62-63 -2.774748976863492 2316 64-65 -2.773011944665459 2316 66-67 -2.490851390354983 2316 68-69 -2.5163629258172513 2316 70-71 -2.7427788032439757 2316 72-73 -5.756326108839826 2316 74-75 -3.4986131463942662 2316 76-77 -2.856265240524504 2316 78-79 -2.8428450614048053 2316 80-81 -2.7383185029656048 2316 82-83 -2.792555030428815 2316 84-85 -2.875817675169813 2316 86-87 -2.6843312780476793 2316 88-89 -2.55674311795811 2316 90-91 -2.508738622745785 2316 92-93 -2.888880013855566 2316 94-95 -3.139477462960407 2316 96-97 -3.085355842177119 2316 98-99 -3.392514331906348 2316 100-101 -3.499375014444478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83056.0 3 16292.0 4 14454.0 5 14604.0 6 16884.0 7 21322.0 8 27018.0 9 32442.0 10 37030.0 11 44188.0 12 45961.0 13 52069.0 14 59375.0 15 70596.0 16 88089.0 17 116481.0 18 164319.0 19 237723.0 20 307761.0 21 227502.0 22 139496.0 23 125570.0 24 131352.0 25 142315.0 26 157212.0 27 177680.0 28 204815.0 29 240474.0 30 281643.0 31 337367.0 32 404802.0 33 500265.0 34 637382.0 35 873026.0 36 1245540.0 37 1407336.0 38 1099771.0 39 190271.0 40 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.39940551655585 46.83440978408625 25.239220571503818 17.526964127854086 2 16.691026030451262 48.59563124970711 18.98896448068596 15.724378239155667 3 42.597164799242776 21.36585421659869 19.873611073060495 16.163369911098037 4 16.503267415752912 19.84863903720484 20.902359270067862 42.74573427697439 5 16.533208991578537 46.0677170895962 21.844353863870865 15.5547200549544 6 42.78145143727677 20.8819157936359 19.73881836510834 16.597814403978994 7 17.381492356720308 20.34974228338019 46.82322126254196 15.445544097357539 8 42.34423357136836 21.07810198175199 20.603798123818354 15.9738663230613 9 41.97490565361831 20.709363172836028 21.21313256277475 16.102598610770915 10-11 16.23958085927501 33.62661805295711 33.684600698400644 16.449200389367235 12-13 16.080715873523125 21.063831922031312 33.63484811467141 29.22060408977416 14-15 16.231571440705682 46.70047066072968 20.60932680981069 16.458631088753947 16-17 29.172143981869525 20.847390744492802 20.73060378257318 29.249861491064493 18-19 16.390284541177717 33.65921052090355 20.713415113134364 29.23708982478437 20-21 29.329079052882044 33.689208230227926 20.74092135291014 16.240791363979888 22-23 29.25918631211546 20.769058275834794 33.63157291033308 16.34018250171667 24-25 16.313681534111186 33.48600401958019 21.159702702937214 29.040611743371407 26-27 16.585784175306042 25.73547327617675 41.14441795451876 16.53432459399845 28-29 26.684775930461228 20.649350519078173 36.21665513939803 16.449218411062567 30-31 24.62223303681414 21.719760501144002 36.63997895300318 17.01802750903868 32-33 19.528169376803245 23.865285622170894 37.85723553079654 18.74930947022932 34-35 19.05935162279887 22.99809612986745 38.51898395320924 19.423568294124436 36-37 18.795961114623704 23.080234918856053 38.65440772788221 19.469396238638026 38-39 19.09260124954356 23.183612480990003 38.43211676049613 19.291669508970312 40-41 19.133579928274568 22.897988952331293 38.62042022590788 19.348010893486265 42-43 19.369766437923413 23.155312704526878 38.56183006907037 18.91309078847934 44-45 18.931048046144458 23.452365701993305 38.2645128600321 19.352073391830142 46-47 19.261214104086168 23.197531264816014 38.18765053622657 19.353604094871244 48-49 19.286820686824147 23.226714859925178 38.40167328972903 19.084791163521643 50-51 19.515447591918235 23.340564635392013 37.636238924466944 19.507748848222803 52-53 19.71450533890072 24.194616067421304 36.651870532328324 19.43900806134965 54-55 20.30026421324838 24.932206331087087 35.212684643332175 19.55484481233236 56-57 20.476676419420965 25.43149254756129 34.09997702451235 19.991854008505396 58-59 20.507169944720875 26.228479720530228 33.30266367119166 19.961686663557234 60-61 20.831828832202 26.36616268073136 32.77738139812147 20.02462708894517 62-63 20.85289726218105 26.30742821618751 32.878295020059376 19.96137950157206 64-65 20.487686188813644 26.069804478924112 32.987716283177534 20.45479304908471 66-67 20.423585308456325 25.80084533095655 33.53045317485104 20.24511618573608 68-69 20.412012387826355 25.519091813518152 33.716898605717525 20.35199719293797 70-71 20.230379431657568 25.579642807950965 34.0443825100311 20.14559525036037 72-73 19.92434647614747 25.247496647033174 34.05009905683609 20.77805781998327 74-75 20.206753619188962 25.613727706823713 33.37413574498407 20.80538292900325 76-77 20.683765647918158 26.10419928060253 33.084138286899915 20.1278967845794 78-79 20.65181917447954 25.812216056093906 32.890480188795976 20.645484580630576 80-81 20.390159422136133 26.1610190658563 32.83638090786257 20.612440604144997 82-83 21.216252252742045 26.37765899796068 32.107459149717485 20.298629599579787 84-85 20.960579084705795 26.68401792877931 31.66373954188395 20.691663444630947 86-87 20.730163390803696 26.945175249736785 31.762564934223008 20.562096425236508 88-89 20.718071817112047 26.684199231281447 31.671837213462712 20.92589173814379 90-91 20.561446128052687 26.614446133770336 31.84053855847616 20.98356917970082 92-93 20.5588499113671 27.17193979533061 31.70737627818066 20.561834015121633 94-95 20.579351575420706 26.629965155046875 31.40613868015493 21.384544589377494 96-97 20.531094461747507 26.456303912154695 31.898870260599693 21.113731365498108 98-99 21.04781183172611 26.61187768422274 31.378201201340204 20.96210928271094 100-101 21.324238148568213 26.428951883822275 31.214469322806416 21.032340644803096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11393.0 1 7072.0 2 2042.0 3 1176.5 4 965.0 5 833.5 6 922.0 7 1148.0 8 1091.5 9 1102.5 10 1538.0 11 10888.0 12 194583.0 13 217939.5 14 58300.0 15 51079.0 16 54414.5 17 58043.0 18 60764.0 19 62620.0 20 64142.5 21 65524.0 22 67865.5 23 73442.5 24 81872.5 25 92873.0 26 103097.5 27 111490.5 28 121476.0 29 132839.0 30 142969.5 31 152732.5 32 164756.5 33 179218.0 34 197939.0 35 212839.0 36 224844.0 37 240073.5 38 253584.0 39 266853.5 40 283016.0 41 294922.0 42 298548.5 43 306652.5 44 320291.5 45 327333.0 46 325667.0 47 325214.0 48 331086.0 49 327828.0 50 313248.0 51 300019.5 52 292178.5 53 290312.5 54 272548.0 55 247368.5 56 240927.0 57 227206.5 58 217932.5 59 186921.5 60 132523.0 61 101348.5 62 76281.5 63 56256.0 64 41081.5 65 28410.0 66 18961.5 67 12262.0 68 7491.5 69 4654.5 70 3222.5 71 2441.5 72 1959.5 73 1668.0 74 1482.0 75 1328.0 76 1213.0 77 1138.0 78 1056.0 79 936.5 80 846.5 81 767.0 82 694.0 83 654.0 84 596.5 85 499.0 86 414.0 87 351.0 88 287.5 89 253.5 90 183.0 91 138.0 92 127.0 93 85.0 94 56.5 95 43.0 96 28.5 97 19.0 98 9.0 99 4.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10062520779767153 2 0.08298872508383094 3 0.08870378770286969 4 0.1934498564416376 5 0.07582985717156136 6 0.09338612848022249 7 0.0981887863653445 8 0.08747053734823501 9 0.07994069168701029 10-11 0.07751429668033068 12-13 0.07097706715820215 14-15 0.07781007623693005 16-17 0.0679591130383238 18-19 0.05998810464861188 20-21 0.05502001074030715 22-23 0.044893320836396404 24-25 0.047049002350595226 26-27 0.04667301138881637 28-29 0.04540968175723938 30-31 0.03584948490240879 32-33 0.03953920954066538 34-35 0.05278411782092884 36-37 0.04767565395356 38-39 0.05217751906925894 40-41 0.04947539735727484 42-43 0.04765058788944141 44-45 0.05136537859181659 46-47 0.04502366436981308 48-49 0.04072232776706287 50-51 0.04496851902875218 52-53 0.04046164070022953 54-55 0.04435690706425856 56-57 0.043349251286691204 58-59 0.05065851558367232 60-61 0.04959571446504407 62-63 0.05376670753437761 64-65 0.05013212823718192 66-67 0.04424160316931304 68-69 0.0451389682647586 70-71 0.05407251351662441 72-73 0.04125874153920071 74-75 0.048833706115838905 76-77 0.04758541612273307 78-79 0.04587591054984517 80-81 0.04525928537252783 82-83 0.04416139176413355 84-85 0.04544477424700541 86-87 0.05011708859871076 88-89 0.042065868803819344 90-91 0.0452492589468804 92-93 0.04162470607533214 94-95 0.04493342653898615 96-97 0.030339964009142496 98-99 0.03705265598010116 100-101 0.025136249098123016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9973644.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.57106633633215 #Duplication Level Percentage of deduplicated Percentage of total 1 91.28547183980993 45.25118180114598 2 5.3267489015514595 5.281052463115836 3 1.3120144234817968 1.951138620619222 4 0.5413557677222844 1.0734233069326953 5 0.3058349696539964 0.7580282784344193 6 0.19958981799462414 0.5936328064720785 7 0.14073339515563657 0.4883413126898115 8 0.10282238766518415 0.40776123198487235 9 0.08761277586600227 0.390875285192742 >10 0.6579272116058491 6.260284293163151 >50 0.019261466941060304 0.641166099804827 >100 0.01322985070707049 1.2680974122680557 >500 0.0033623599298940503 1.1570393645583477 >1k 0.0034584273559196805 3.4857141282958555 >5k 3.842697062132978E-4 1.3706224545541716 >10k+ 1.921348531066489E-4 29.621641140767963 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1192196 11.953464551171066 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 28752 0.28827979021509087 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGCCAGCTTTCCAAGAA 12603 0.12636304243464075 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10490 0.10517720504160766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0026425647436384E-5 0.0 0.0 0.0 0.0 3 1.0026425647436384E-5 0.0 0.0 0.0 0.0 4 1.0026425647436384E-5 0.0 0.0 0.0 0.0 5 1.0026425647436384E-5 0.0 0.0 0.0 0.0 6 1.0026425647436384E-5 0.0 0.0 0.0 0.0 7 1.0026425647436384E-5 0.0 0.0 0.0 0.0 8 1.0026425647436384E-5 0.0 0.0 0.0 0.0 9 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 10-11 2.0052851294872767E-5 0.0 0.0 1.0026425647436384E-5 0.0 12-13 2.0052851294872767E-5 0.0 0.0 1.0026425647436384E-5 0.0 14-15 2.0052851294872767E-5 0.0 0.0 1.0026425647436384E-5 0.0 16-17 2.0052851294872767E-5 0.0 0.0 1.0026425647436384E-5 0.0 18-19 2.0052851294872767E-5 0.0 0.0 2.0052851294872767E-5 0.0 20-21 3.5092489766027344E-5 0.0 0.0 2.0052851294872767E-5 0.0 22-23 5.0132128237181914E-5 0.0 0.0 2.0052851294872767E-5 0.0 24-25 6.01585538846183E-5 0.0 0.0 2.5066064118590957E-5 0.0 26-27 6.51717667083365E-5 0.0 0.0 3.007927694230915E-5 0.0 28-29 7.018497953205469E-5 0.0 0.0 3.007927694230915E-5 0.0 30-31 7.519819235577288E-5 0.0 0.0 3.007927694230915E-5 0.0 32-33 8.021140517949107E-5 0.0 0.0 3.007927694230915E-5 0.0 34-35 9.023783082692745E-5 0.0 0.0 4.0105702589745534E-5 0.0 36-37 1.0026425647436383E-4 0.0 0.0 5.0132128237181914E-5 0.0 38-39 1.1530389494551841E-4 0.0 0.0 5.0132128237181914E-5 0.0 40-41 1.4036995906410938E-4 0.0 0.0 5.0132128237181914E-5 0.0 42-43 1.5540959753526396E-4 0.0 0.0 5.0132128237181914E-5 0.0 44-45 1.7546244883013672E-4 0.0 0.0 6.01585538846183E-5 0.0 46-47 1.804756616538549E-4 0.0 0.0 6.01585538846183E-5 0.0 48-49 1.9551530012500948E-4 0.0 0.0 6.01585538846183E-5 0.0 50-51 2.0554172577244583E-4 0.0 0.0 6.51717667083365E-5 0.0 52-53 2.1055493859616404E-4 1.0026425647436384E-5 0.0 8.021140517949107E-5 0.0 54-55 2.2058136424360042E-4 1.0026425647436384E-5 0.0 8.021140517949107E-5 0.0 56-57 2.35621002714755E-4 1.0026425647436384E-5 0.0 8.522461800320926E-5 0.0 58-59 2.5567385400962776E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 60-61 2.8575313095193693E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 62-63 3.1081919507052787E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 64-65 3.308720463654006E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 66-67 3.810041746025826E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 68-69 4.060702387211735E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 70-71 4.662287926057918E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 72-73 5.113477080192555E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 74-75 5.815326875513102E-4 1.0026425647436384E-5 0.0 9.023783082692745E-5 0.0 76-77 6.717705183782376E-4 1.0026425647436384E-5 0.0 1.0527746929808203E-4 5.013212823718192E-6 78-79 7.269158594391378E-4 1.0026425647436384E-5 0.0 1.203171077692366E-4 1.0026425647436384E-5 80-81 8.522461800320926E-4 1.0026425647436384E-5 0.0 1.253303205929548E-4 1.0026425647436384E-5 82-83 9.876029262724838E-4 1.0026425647436384E-5 0.0 1.30343533416673E-4 1.0026425647436384E-5 84-85 0.0011129332468654387 1.0026425647436384E-5 0.0 1.353567462403912E-4 1.0026425647436384E-5 86-87 0.0014187392291122483 1.0026425647436384E-5 0.0 1.4036995906410938E-4 2.0052851294872767E-5 88-89 0.0017997434037148308 1.0026425647436384E-5 0.0 1.4036995906410938E-4 2.0052851294872767E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 259065 0.0 91.315125 5 GTATCAA 260015 0.0 91.07013 9 AAGCAGT 261560 0.0 90.889725 1 GGTATCA 260670 0.0 90.8855 8 TGGTATC 260885 0.0 90.729095 7 GTGGTAT 261600 0.0 90.576935 6 GCAGTGG 262090 0.0 90.49533 3 AGCAGTG 263270 0.0 90.258705 2 CAGTGGT 262105 0.0 90.12844 4 TCAACGC 258890 0.0 46.272957 12-13 ATCAACG 258730 0.0 46.174873 10-11 CAACGCA 259120 0.0 46.151207 12-13 AACGCAG 260355 0.0 46.12216 14-15 CGCAGAG 261130 0.0 46.00926 16-17 ACGCAGA 260380 0.0 45.973583 14-15 TATCAAC 260600 0.0 45.848087 10-11 CAGAGTA 259970 0.0 45.666615 18-19 AGAGTAC 254905 0.0 45.639515 18-19 GAGTACT 152010 0.0 45.4472 20-21 GCAGAGT 262940 0.0 45.31316 16-17 >>END_MODULE