FastQCFastQC Report
Sat 18 Jun 2016
SRR578577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9973644
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1318021.3215029531834102No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140340.1407108575361222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3950.036.074733
CGCGTAG2900.023.74991254-55
TCGCGTA3050.023.36191652-53
ACTCGCG3100.022.98528350-51
CTCGCGT3500.021.0368552-53
CGTACGC3400.019.55772220-21
CGTAGCG3650.019.51949756-57
ATCAACG75150.017.9812210-11
CAACGCA75700.017.94469312-13
TCAACGC74700.017.13578610-11
ACGTACG4200.016.9633320-21
CGCTAGT4350.016.37900490-91
AACGCAG79700.015.94157812-13
GTATCAA100000.015.0090429
AGTGGTA98300.014.9789235
ACGCAGA90050.014.92697614-15
GGTATCA101900.014.4495198
AAGCAGT114350.013.9765781
CGACGAC5550.013.693360-61
CGCAGAG95900.013.223968514-15
Produced by FastQC (version 0.11.5)