##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9973644 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49403126881208 31.0 31.0 34.0 30.0 34.0 2 31.811104246351686 31.0 31.0 34.0 30.0 34.0 3 31.884516732299648 31.0 31.0 34.0 30.0 34.0 4 35.55420285705004 37.0 35.0 37.0 33.0 37.0 5 35.3715926696401 37.0 35.0 37.0 33.0 37.0 6 35.37173574673409 37.0 35.0 37.0 32.0 37.0 7 35.34881363321169 37.0 35.0 37.0 32.0 37.0 8 35.3348171440649 37.0 35.0 37.0 32.0 37.0 9 36.90520706373719 39.0 37.0 39.0 33.0 39.0 10-11 36.873443948871646 39.0 37.0 39.0 32.5 39.0 12-13 36.85424028569698 39.0 37.0 39.0 32.0 39.0 14-15 38.03940280001973 40.0 37.0 41.0 33.0 41.0 16-17 37.99093059668061 40.0 37.0 41.0 33.0 41.0 18-19 37.93162739716797 40.0 37.0 41.0 33.0 41.0 20-21 37.88349945115346 40.0 37.0 41.0 32.0 41.0 22-23 37.79719413486184 40.0 37.0 41.0 32.0 41.0 24-25 37.71344741199907 40.0 37.0 41.0 32.0 41.0 26-27 37.53068346935183 39.0 36.0 41.0 32.0 41.0 28-29 37.362287143996724 39.0 36.0 41.0 31.5 41.0 30-31 37.2351978374203 39.0 36.0 41.0 31.0 41.0 32-33 37.107324765151034 39.0 36.0 41.0 31.0 41.0 34-35 36.95658998857388 39.0 36.0 40.0 30.5 41.0 36-37 36.80704950968773 39.0 35.0 40.0 30.0 41.0 38-39 36.64791725070596 39.0 35.0 40.0 30.0 41.0 40-41 36.482933168659315 38.0 35.0 40.0 30.0 41.0 42-43 36.428083356494376 38.0 35.0 40.0 30.0 41.0 44-45 36.468875718844586 38.0 35.0 40.0 30.0 41.0 46-47 36.345551585759424 38.0 35.0 40.0 30.0 41.0 48-49 36.20724260861928 38.0 35.0 40.0 29.5 41.0 50-51 36.01490543476386 38.0 35.0 40.0 29.0 41.0 52-53 36.08020624157028 38.0 35.0 40.0 29.0 41.0 54-55 36.07557493529947 38.0 35.0 40.0 29.0 41.0 56-57 35.92124027085787 38.0 34.5 40.0 28.0 41.0 58-59 35.71607548855765 38.0 34.0 40.0 28.0 41.0 60-61 35.46306821258108 38.0 34.0 40.0 28.0 41.0 62-63 35.184715385871 37.5 34.0 40.0 28.0 41.0 64-65 34.86544200895881 37.0 33.5 40.0 27.0 41.0 66-67 34.525642082272036 36.5 33.0 39.5 26.0 41.0 68-69 34.16045273923954 36.0 33.0 39.0 26.0 41.0 70-71 33.77612485466696 35.5 32.0 39.0 26.0 40.0 72-73 33.38276366190732 35.0 32.0 38.5 26.0 40.0 74-75 32.97123824551989 35.0 32.0 37.5 25.5 39.5 76-77 31.61591781298791 34.0 30.5 36.0 23.5 39.0 78-79 32.26099242162644 35.0 31.0 36.0 25.0 39.0 80-81 32.132998631192365 35.0 31.0 36.0 25.0 39.0 82-83 31.81809863075121 34.5 31.0 36.0 24.5 37.0 84-85 31.488540246674134 34.0 31.0 35.0 24.0 37.0 86-87 31.139096452610502 34.0 31.0 35.0 23.0 36.5 88-89 30.81785679336459 34.0 31.0 35.0 21.0 36.0 90-91 30.493826378803973 34.0 30.5 35.0 20.0 36.0 92-93 30.130068809353933 34.0 30.0 35.0 18.5 35.5 94-95 29.759155179390802 34.0 30.0 35.0 16.5 35.0 96-97 29.370455672971687 34.0 30.0 35.0 4.5 35.0 98-99 28.89325616595098 34.0 29.0 35.0 2.0 35.0 100-101 27.76411184317387 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4132773895613653 1101 2 0.4027728606455021 1101 3 0.4169801174759904 1101 4 0.3420445032747068 1101 5 0.3664835372145703 1101 6 0.28032937009048453 1101 7 0.3311589165400832 1101 8 0.32571353631627176 1101 9 0.3893110584624182 1101 10-11 0.40946322657711676 1101 12-13 0.37022343303345195 1101 14-15 0.5322813544024569 1101 16-17 0.48941928625460207 1101 18-19 0.47002495570541925 1101 20-21 0.4827507323509437 1101 22-23 0.5761653232494268 1101 24-25 0.5191376669133803 1101 26-27 0.5175451943434197 1101 28-29 0.6132502069571117 1101 30-31 0.6639734400597206 1101 32-33 0.5790113924021583 1101 34-35 0.6331025741582081 1101 36-37 0.5643104510986703 1101 38-39 0.625169010537654 1101 40-41 0.6256726749632051 1101 42-43 0.6116592408552322 1101 44-45 0.57126007346276 1101 46-47 0.6161166602047672 1101 48-49 0.5417518104637153 1101 50-51 0.5642010782242579 1101 52-53 0.6492885587226809 1101 54-55 0.6656331628525365 1101 56-57 0.6618535136312573 1101 58-59 0.5673878692698082 1101 60-61 0.6267026741582313 1101 62-63 0.5376092495101119 1101 64-65 0.6188407783805729 1101 66-67 0.5750741970821096 1101 68-69 0.6431453928126913 1101 70-71 0.5883935646365615 1101 72-73 0.6059349471303648 1101 74-75 0.6166284210409358 1101 76-77 0.5174796355416014 1101 78-79 0.6083587777741712 1101 80-81 0.6285115215381154 1101 82-83 0.6123300706140213 1101 84-85 0.6966273004064263 1101 86-87 0.596777622636651 1101 88-89 0.6286670717318117 1101 90-91 0.7538255839683785 1101 92-93 0.8230005630004591 1101 94-95 0.8183344681599998 1101 96-97 0.7738172711419189 1101 98-99 0.9053996193118685 1101 100-101 1.1108886507467943 1102 1 0.30356174663883806 1102 2 0.2935300678293551 1102 3 0.3476832285353062 1102 4 0.23246404644451246 1102 5 0.29631350114365773 1102 6 0.17191904005769487 1102 7 0.16558798281906206 1102 8 0.1510096294020613 1102 9 0.35176796086709317 1102 10-11 0.26616842864316936 1102 12-13 0.22367847588635925 1102 14-15 0.446872389765268 1102 16-17 0.39399272493455584 1102 18-19 0.42667003088531175 1102 20-21 0.4239647528596251 1102 22-23 0.3725689836197148 1102 24-25 0.28020326706915455 1102 26-27 0.3695104993107563 1102 28-29 0.31109310521519973 1102 30-31 0.2555265643986715 1102 32-33 0.36688080817935287 1102 34-35 0.40994491540622846 1102 36-37 0.4204849593954023 1102 38-39 0.4538144635638801 1102 40-41 0.37821642057624416 1102 42-43 0.39792985819018867 1102 44-45 0.3321283063415663 1102 46-47 0.35852365781603623 1102 48-49 0.4138025015534126 1102 50-51 0.3563215263014854 1102 52-53 0.3691455040447238 1102 54-55 0.3648340768968765 1102 56-57 0.38333167796476886 1102 58-59 0.4259658164019271 1102 60-61 0.3938138156409181 1102 62-63 0.422694896084586 1102 64-65 0.4118548964915334 1102 66-67 0.4297276007855757 1102 68-69 0.3576576981408053 1102 70-71 0.3463445330505266 1102 72-73 0.4335431902850857 1102 74-75 0.3052869886442764 1102 76-77 0.1981770736848283 1102 78-79 0.26429439907254704 1102 80-81 0.31236335203853116 1102 82-83 0.39351412045439815 1102 84-85 0.20248590659765142 1102 86-87 0.2668180712311745 1102 88-89 0.4133320630441162 1102 90-91 0.4954500605389569 1102 92-93 0.39242835071459936 1102 94-95 0.23753175073479582 1102 96-97 0.18673008723730433 1102 98-99 0.3810295650153357 1102 100-101 0.5650655756857006 1103 1 0.21018082431545793 1103 2 0.23497669836950408 1103 3 0.2802164512001575 1103 4 0.12425397690605422 1103 5 0.2429438914855453 1103 6 0.0937167459292425 1103 7 0.1287643046172917 1103 8 0.12411188815796237 1103 9 0.2974356181476665 1103 10-11 0.263353720037081 1103 12-13 0.2391286202319023 1103 14-15 0.349104538855471 1103 16-17 0.2963361061115535 1103 18-19 0.269944759648304 1103 20-21 0.2702795856361817 1103 22-23 0.2760040122262595 1103 24-25 0.33891470843505545 1103 26-27 0.2647235366960601 1103 28-29 0.3168235725960997 1103 30-31 0.3351799840919867 1103 32-33 0.26424624012508247 1103 34-35 0.3059072101307194 1103 36-37 0.4000561717103963 1103 38-39 0.35710168207977944 1103 40-41 0.32467931112520176 1103 42-43 0.3318600360191084 1103 44-45 0.2625877883898369 1103 46-47 0.2913644453154376 1103 48-49 0.24531327987521223 1103 50-51 0.3017490221462751 1103 52-53 0.39217279811675354 1103 54-55 0.2736415878306744 1103 56-57 0.2529411413340483 1103 58-59 0.2768944113245908 1103 60-61 0.3379236594314179 1103 62-63 0.2626600470593061 1103 64-65 0.25389125633567744 1103 66-67 0.3004595969023214 1103 68-69 0.27475318551671535 1103 70-71 0.2349749329401405 1103 72-73 0.29062060508100984 1103 74-75 0.28459140374567227 1103 76-77 0.2297788460955985 1103 78-79 0.24965881815961666 1103 80-81 0.2827844106540027 1103 82-83 0.3182919973426124 1103 84-85 0.3381848528774931 1103 86-87 0.36996322327692255 1103 88-89 0.37859255206261366 1103 90-91 0.4293943951789245 1103 92-93 0.3217361574583357 1103 94-95 0.3598199024716813 1103 96-97 0.40473108519510603 1103 98-99 0.5385799910769258 1103 100-101 0.6842592233033713 1104 1 0.13071344392335504 1104 2 0.14590391424015792 1104 3 0.18264499261074363 1104 4 0.12661336372612197 1104 5 0.1337099628492595 1104 6 0.20778690829325797 1104 7 0.14687406683260207 1104 8 0.10814936712998247 1104 9 0.1868891526181713 1104 10-11 0.2035318254228926 1104 12-13 0.1658546266358556 1104 14-15 0.18294498725852293 1104 16-17 0.2038822064789585 1104 18-19 0.23271858216599384 1104 20-21 0.25757446375698834 1104 22-23 0.2916079496215076 1104 24-25 0.2691445512722481 1104 26-27 0.2621690418708127 1104 28-29 0.2511841961661432 1104 30-31 0.2139200727418924 1104 32-33 0.17292255601660145 1104 34-35 0.2259999579162013 1104 36-37 0.2812601042352796 1104 38-39 0.3730684012022749 1104 40-41 0.3527480306911812 1104 42-43 0.23013361317187986 1104 44-45 0.24401771208435008 1104 46-47 0.1834156481421303 1104 48-49 0.17346687404424443 1104 50-51 0.21291567366838393 1104 52-53 0.2167279991836466 1104 54-55 0.23293939290336851 1104 56-57 0.2789693176748713 1104 58-59 0.2892045997470021 1104 60-61 0.25118375594008313 1104 62-63 0.2215744309756218 1104 64-65 0.29737511926431637 1104 66-67 0.28802859184022367 1104 68-69 0.3345786146091001 1104 70-71 0.20487989462043288 1104 72-73 0.25035214592960386 1104 74-75 0.2756566852790243 1104 76-77 0.28497997550516274 1104 78-79 0.34894801391665453 1104 80-81 0.4181987771046991 1104 82-83 0.29541797010794824 1104 84-85 0.30023419392380646 1104 86-87 0.40509704737343455 1104 88-89 0.34751340441064116 1104 90-91 0.5235160299325052 1104 92-93 0.4819761986356319 1104 94-95 0.49465496554177335 1104 96-97 0.4826969086471635 1104 98-99 0.5260710303331599 1104 100-101 0.6497736657871869 1105 1 0.13951639370906932 1105 2 0.148387879527224 1105 3 0.06758892492322843 1105 4 0.10558083111124716 1105 5 0.0806779544546643 1105 6 0.13305050735733204 1105 7 0.15071874195755441 1105 8 0.13771404170064017 1105 9 0.12485901231111285 1105 10-11 0.09086390098137542 1105 12-13 0.12564718223622862 1105 14-15 0.1187821431541991 1105 16-17 0.07645234379047139 1105 18-19 0.11452550355417657 1105 20-21 0.11816679065226054 1105 22-23 0.10852530886039347 1105 24-25 0.06419963272982443 1105 26-27 0.08784835011822878 1105 28-29 0.013806796782240838 1105 30-31 0.07909707890162565 1105 32-33 0.1140079219565564 1105 34-35 0.10751909991680009 1105 36-37 0.1863870883411991 1105 38-39 0.18749307903383539 1105 40-41 0.2050671746966728 1105 42-43 0.13872747557850573 1105 44-45 0.10005167392182557 1105 46-47 0.14261298302159275 1105 48-49 0.20666318554430774 1105 50-51 0.1677375898792306 1105 52-53 0.12237190634653672 1105 54-55 0.11411412837556867 1105 56-57 0.1175626545344528 1105 58-59 0.07129027850906766 1105 60-61 -0.01469815769389271 1105 62-63 0.04353827643501518 1105 64-65 0.1348389353874211 1105 66-67 0.06731317121388969 1105 68-69 0.052519121062495344 1105 70-71 0.03600983697389637 1105 72-73 0.09794973257042727 1105 74-75 0.17147063307636046 1105 76-77 0.07385536454265207 1105 78-79 0.08247591021893186 1105 80-81 0.06510714160230435 1105 82-83 0.0843809810310745 1105 84-85 0.10811910958861404 1105 86-87 0.11651428410753795 1105 88-89 0.1566063596036642 1105 90-91 0.20272355364330252 1105 92-93 0.20758025709710282 1105 94-95 0.19114882231701458 1105 96-97 0.2761101347128836 1105 98-99 0.4609176258792296 1105 100-101 0.4542524211561698 1106 1 -0.08217028832142503 1106 2 -0.009105853354864024 1106 3 -0.11557269941854642 1106 4 -0.0025924499582998806 1106 5 -0.03748560709769322 1106 6 0.09038123196332748 1106 7 0.014572326020221738 1106 8 0.03129927190149573 1106 9 -0.058597395245271855 1106 10-11 -0.030069072276198483 1106 12-13 -0.04210088297005399 1106 14-15 0.15399838196132265 1106 16-17 0.04496473338620177 1106 18-19 -0.022519856175790665 1106 20-21 0.07183750724169613 1106 22-23 0.022932745518076558 1106 24-25 0.13304093911622772 1106 26-27 0.18006007570807725 1106 28-29 0.14822740349725905 1106 30-31 0.05270753002256612 1106 32-33 0.023547029441623124 1106 34-35 0.0507578817571428 1106 36-37 0.10602034574777264 1106 38-39 0.012144250990452576 1106 40-41 -0.030249308251036666 1106 42-43 -0.007988208836877675 1106 44-45 0.01851451777670121 1106 46-47 0.026911761128801004 1106 48-49 0.06754136660396881 1106 50-51 0.015744438160325558 1106 52-53 -0.10045434631480532 1106 54-55 -0.09107186986793181 1106 56-57 -0.06732555986307176 1106 58-59 0.05386122094527934 1106 60-61 0.2295283970384432 1106 62-63 0.057259323316998234 1106 64-65 0.06457450428408151 1106 66-67 0.013091669589393007 1106 68-69 0.09718457855280604 1106 70-71 0.01968710884597158 1106 72-73 -0.00430629973971719 1106 74-75 -0.022828929582551893 1106 76-77 -0.09242304199195672 1106 78-79 0.006372127787386717 1106 80-81 0.12919623990328688 1106 82-83 0.15775614100897428 1106 84-85 0.31464974019713665 1106 86-87 0.4730571663645513 1106 88-89 0.3386915120929963 1106 90-91 0.3710074193191275 1106 92-93 0.2622918432524024 1106 94-95 0.4288482230087567 1106 96-97 0.4490334338159627 1106 98-99 0.5200653870810292 1106 100-101 0.44168897843922394 1107 1 0.02134020864396291 1107 2 0.05835214061225713 1107 3 0.017584506720936588 1107 4 0.11668731971013102 1107 5 -0.05038239804272138 1107 6 -0.053196821928771953 1107 7 0.0024443837807908153 1107 8 -0.012524902413950656 1107 9 -0.07511391297442316 1107 10-11 -0.11984245573545138 1107 12-13 -0.10707011286955037 1107 14-15 -0.040618970437094504 1107 16-17 0.0192026484494221 1107 18-19 -0.10000528883490034 1107 20-21 -0.1654689177329587 1107 22-23 -0.22321957859364971 1107 24-25 -0.1861187988937303 1107 26-27 -0.21350727729605268 1107 28-29 -0.09452557155150032 1107 30-31 -0.05847156649944196 1107 32-33 -0.09790522238862565 1107 34-35 -0.12906357493656628 1107 36-37 -0.10564836687483137 1107 38-39 -0.030179981877019202 1107 40-41 -0.0386518271996934 1107 42-43 -0.06286084807966574 1107 44-45 -0.06186439460927318 1107 46-47 -0.025091122053844117 1107 48-49 -0.0817682242183011 1107 50-51 -0.03210074623818571 1107 52-53 -0.06786055202221775 1107 54-55 -0.06361541036776686 1107 56-57 -0.01626445577371527 1107 58-59 -0.03336222167170888 1107 60-61 0.13615093431993586 1107 62-63 0.0685247336051944 1107 64-65 0.027937995277163452 1107 66-67 -0.05257119994458037 1107 68-69 0.08511740928090461 1107 70-71 0.061693848386354944 1107 72-73 -0.06149870978159555 1107 74-75 -0.02242481963396159 1107 76-77 -0.018197342093312585 1107 78-79 0.010997031378437327 1107 80-81 0.1577194567921154 1107 82-83 0.11998029028838175 1107 84-85 0.18847920438550858 1107 86-87 0.19898582172730883 1107 88-89 0.20515402062342147 1107 90-91 0.15278168711591533 1107 92-93 0.21768922981289762 1107 94-95 0.1860841432649316 1107 96-97 0.2446056890381989 1107 98-99 0.2647810665066537 1107 100-101 0.4649138424571859 1108 1 0.03358257231659678 1108 2 0.019101785138811778 1108 3 0.0043438008769882686 1108 4 0.0284565207975902 1108 5 -0.019105241514282056 1108 6 0.0056073472598967555 1108 7 0.04346820117508088 1108 8 -0.02960418282329158 1108 9 -0.13704685898429858 1108 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Count 2 139.0 3 86.0 4 242.0 5 657.0 6 1157.0 7 2059.0 8 3803.0 9 5976.0 10 8349.0 11 10719.0 12 13622.0 13 16573.0 14 19111.0 15 22080.0 16 25717.0 17 30173.0 18 35246.0 19 40525.0 20 43543.0 21 46311.0 22 51820.0 23 61933.0 24 75834.0 25 96733.0 26 123475.0 27 156002.0 28 196467.0 29 245447.0 30 299606.0 31 365046.0 32 444463.0 33 552208.0 34 709727.0 35 985043.0 36 1273232.0 37 1818069.0 38 1773993.0 39 417741.0 40 717.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.729014341103095 30.74099481424523 29.692015608473206 22.83797523617847 2 22.02463813627196 29.92075915282318 28.02958477362938 20.025017937275482 3 21.279363891472364 28.81514519668037 28.36752545007622 21.537965461771044 4 21.476624398435472 28.678283135819 28.57359433908442 21.27149812666111 5 21.954618237528837 28.700249959643497 28.791470691153954 20.55366111167371 6 21.893398690293996 28.840503508484854 27.734767288151364 21.531330513069786 7 22.172006540437977 28.863703176090905 28.261656421664938 20.702633861806177 8 21.393145116818165 29.052630840156336 28.165921701448422 21.38830234157708 9 21.02664618998456 28.751072454026477 28.83049195472738 21.391789401261587 10-11 21.134609568318307 29.121057035031416 28.194478972984115 21.549854423666158 12-13 21.119098608201636 29.264723030491467 28.054974496279844 21.56120386502705 14-15 21.31603587315626 29.253176986560025 27.89193896732703 21.538848172956687 16-17 21.444970363891073 29.03112944476462 27.9279870025439 21.595913188800402 18-19 21.40092749464401 29.091362558864525 27.90660984690398 21.601100099587487 20-21 21.50587993008163 29.002297608679207 27.925613446377422 21.56620901486174 22-23 21.449264929007533 28.982882207814338 27.933013546674612 21.634839316503516 24-25 21.444995429955192 28.928288396898864 27.90538242592176 21.721333747224183 26-27 21.6870533979356 28.83256109802997 27.948977324636815 21.53140817939762 28-29 21.64128093910614 28.913308235046863 27.87901163314201 21.566399192704985 30-31 21.62412691078974 28.893705656212354 27.862422136113985 21.61974529688392 32-33 21.659281262503306 28.921132746922666 27.755797835959857 21.66378815461417 34-35 21.668762862679174 28.909627816253714 27.775215463349223 21.64639385771789 36-37 21.662993892535148 28.942681712833394 27.755634629752336 21.63868976487912 38-39 21.70272839911877 28.899593899748062 27.75898384948748 21.638693851645698 40-41 21.68163913567268 28.991778552846824 27.708944026077614 21.617638285402883 42-43 21.719415546604694 28.95782036412657 27.707172716350705 21.615591372918033 44-45 21.68536279032483 28.992143903226836 27.702322678846432 21.620170627601905 46-47 21.717498128573364 29.00487932099513 27.667409020539598 21.610213529891915 48-49 21.72169922351776 29.013549145564717 27.633927857446572 21.630823773470954 50-51 21.770595381990667 28.988281131123557 27.61481363532606 21.626309851559725 52-53 21.704287358556094 29.03827544436072 27.599902353674526 21.657534843408655 54-55 21.729375135269116 29.070362701464813 27.536317548640106 21.663944614625965 56-57 21.728683117223753 29.0485202800501 27.55136938916208 21.67142721356407 58-59 21.728653904557802 29.129127292455642 27.505782994551936 21.636435808434616 60-61 21.753135956949333 29.107964109671876 27.469901516014783 21.66899841736401 62-63 21.748545328703624 29.15752541294437 27.462358221373133 21.631571036978876 64-65 21.758406985725458 29.177115715190173 27.482320693675614 21.582156605408752 66-67 21.727908170130387 29.17495273008452 27.49260696316085 21.604532136624243 68-69 21.702789110943346 29.217687635837354 27.46975012770021 21.60977312551909 70-71 21.72251948998741 29.27463863429942 27.41582120692913 21.587020668784042 72-73 21.685025717979265 29.244920449681366 27.441638851770612 21.62841498056876 74-75 21.768276787271972 29.28253946927783 27.399233775635228 21.549949967814968 76-77 21.713152183895875 29.339903249003076 27.441534909407235 21.505409657693818 78-79 21.696428105915953 29.3231691445975 27.393493290917544 21.586909458569004 80-81 21.70040921391294 29.345039664326983 27.373942768807098 21.58060835295298 82-83 21.715805006754064 29.38566735122201 27.348474765074194 21.550052876949735 84-85 21.688942657340274 29.415973451192123 27.367802526873238 21.527281364594366 86-87 21.68761894792506 29.448460636753225 27.32286666793003 21.54105374739168 88-89 21.679324124964953 29.486557904118015 27.325165321844626 21.50895264907241 90-91 21.63250211157625 29.480792877831778 27.340611085755263 21.54609392483671 92-93 21.67217705569261 29.499843837496748 27.305472405358756 21.522506701451885 94-95 21.649716023184578 29.55005224535721 27.310477792226457 21.489753939231754 96-97 21.629833353971286 29.553904408015814 27.298751144904994 21.51751109310791 98-99 21.618903782809973 29.59423356197594 27.273812861176914 21.513049794037165 100-101 21.711941163324173 29.572830108316488 27.181301757041034 21.533926971318305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 145092.0 1 81546.5 2 13235.5 3 7720.5 4 6363.5 5 5359.5 6 4891.5 7 4986.5 8 4794.0 9 4379.5 10 4371.5 11 5033.0 12 8765.5 13 8581.5 14 5481.5 15 5991.0 16 6646.5 17 7775.5 18 9699.0 19 11804.0 20 14579.0 21 18683.0 22 24905.5 23 34070.5 24 46551.0 25 62469.5 26 78815.5 27 93477.5 28 108169.0 29 123221.5 30 139508.5 31 157706.0 32 176847.5 33 196756.0 34 216058.5 35 234420.0 36 254613.0 37 276170.0 38 293174.0 39 306025.0 40 322387.5 41 335878.0 42 343316.5 43 356335.0 44 369941.5 45 378248.0 46 379973.0 47 376097.5 48 376524.0 49 377203.5 50 369557.0 51 353243.5 52 334847.0 53 316137.0 54 290285.0 55 263597.0 56 238438.5 57 212239.5 58 191532.0 59 167219.5 60 134637.5 61 104036.5 62 77949.0 63 57441.5 64 42044.0 65 27713.0 66 16805.0 67 11120.0 68 6493.5 69 3046.5 70 1464.5 71 697.5 72 343.0 73 203.0 74 132.0 75 93.0 76 57.5 77 34.0 78 29.0 79 24.0 80 20.0 81 16.0 82 12.0 83 8.5 84 6.0 85 5.5 86 5.5 87 3.5 88 2.5 89 1.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1447615334976865 2 0.0 3 0.0 4 0.0027572670530450058 5 3.4089847201283703E-4 6 0.0010828539699231294 7 0.0 8 2.406342155384732E-4 9 2.306077898910368E-4 10-11 1.0527746929808203E-4 12-13 1.0026425647436384E-5 14-15 2.5066064118590957E-5 16-17 0.0 18-19 1.5039638471154575E-5 20-21 1.3535674624039117E-4 22-23 1.5540959753526396E-4 24-25 0.0 26-27 0.0 28-29 0.005875485429397721 30-31 0.0015039638471154575 32-33 3.058059822468097E-4 34-35 2.2058136424360042E-4 36-37 2.957795565993733E-4 38-39 0.0012182107161635206 40-41 0.0033989582944809337 42-43 5.163609208429737E-4 44-45 5.2638734649041E-4 46-47 0.006391846350240694 48-49 0.006552269160599677 50-51 0.00712377542250355 52-53 0.009209271957170318 54-55 0.004707406841471382 56-57 0.0 58-59 5.0132128237181914E-5 60-61 9.374707980353018E-4 62-63 8.522461800320925E-4 64-65 4.862816439006646E-4 66-67 7.018497953205469E-4 68-69 8.973650954455563E-4 70-71 0.001258316418753266 72-73 0.00271716135045526 74-75 6.416912414359285E-4 76-77 0.0 78-79 0.0 80-81 9.525104365064564E-5 82-83 1.654360231827003E-4 84-85 1.3535674624039117E-4 86-87 0.0026770556478655145 88-89 0.001438792080407121 90-91 5.21374133666692E-4 92-93 5.915591131987466E-4 94-95 3.058059822468097E-4 96-97 0.0010928803955705657 98-99 0.0 100-101 1.0026425647436384E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9973644.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.34073867682025 #Duplication Level Percentage of deduplicated Percentage of total 1 90.31229030022651 62.62320921013135 2 6.047717086201746 8.387063401313121 3 1.4319991693077954 2.9788764055318655 4 0.5677294578555002 1.5746711990516433 5 0.29848593647904537 1.0348617660049728 6 0.17997036040770809 0.7487566638362426 7 0.12276672933761838 0.5958914988045403 8 0.10031176100381749 0.5564553284781887 9 0.07457947122511563 0.46542560623885604 >10 0.7987968930213288 12.062092060074079 >50 0.05973939394703118 2.6914658705320975 >100 0.005269054425716893 0.46515013254967735 >500 4.9198080155631995E-5 0.02466464771678932 >1k 4.9198080155618144E-5 0.11458997466206361 >5k 1.4759424046685443E-4 0.7017314018453851 >10k+ 9.839616031123629E-5 4.975094833229148 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131802 1.3215029531834102 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14034 0.1407108575361222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0026425647436384E-5 0.0 5 0.0 0.0 0.0 1.0026425647436384E-5 0.0 6 0.0 0.0 0.0 1.0026425647436384E-5 0.0 7 0.0 0.0 0.0 1.0026425647436384E-5 0.0 8 0.0 0.0 0.0 1.0026425647436384E-5 0.0 9 0.0 0.0 0.0 1.0026425647436384E-5 0.0 10-11 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 12-13 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 14-15 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 16-17 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 18-19 1.0026425647436384E-5 0.0 0.0 1.0026425647436384E-5 0.0 20-21 2.5066064118590957E-5 0.0 0.0 1.0026425647436384E-5 0.0 22-23 3.5092489766027344E-5 0.0 0.0 1.0026425647436384E-5 0.0 24-25 5.0132128237181914E-5 0.0 0.0 1.0026425647436384E-5 0.0 26-27 5.5145341060900104E-5 0.0 0.0 1.0026425647436384E-5 0.0 28-29 6.01585538846183E-5 0.0 0.0 1.0026425647436384E-5 0.0 30-31 6.51717667083365E-5 0.0 0.0 1.0026425647436384E-5 0.0 32-33 7.018497953205469E-5 0.0 0.0 1.0026425647436384E-5 0.0 34-35 9.023783082692745E-5 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 36-37 9.525104365064564E-5 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 38-39 1.0527746929808203E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 40-41 1.30343533416673E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 42-43 1.353567462403912E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 44-45 1.4538317188782755E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 46-47 1.5039638471154576E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 48-49 1.654360231827003E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 50-51 1.7546244883013672E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 52-53 1.8548887447757307E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 54-55 1.9551530012500948E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 56-57 2.0052851294872766E-4 0.0 0.0 1.0026425647436384E-5 1.0026425647436384E-5 58-59 2.1556815141988224E-4 0.0 0.0 1.5039638471154575E-5 1.0026425647436384E-5 60-61 2.456474283621914E-4 5.013212823718192E-6 0.0 2.0052851294872767E-5 1.0026425647436384E-5 62-63 2.60687066833346E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 64-65 2.757267053045006E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 66-67 3.108191950705279E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 68-69 3.3588525918911886E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 70-71 3.9604381307373714E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 72-73 4.4617594131091907E-4 1.0026425647436384E-5 0.0 2.0052851294872767E-5 1.0026425647436384E-5 74-75 4.8628164390066464E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 76-77 5.614798362564375E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 78-79 6.166251773173376E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 80-81 7.168894337917014E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 82-83 8.321933287372198E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 84-85 9.474972236827382E-4 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 86-87 0.0012733560572244207 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 88-89 0.001649347019003285 1.0026425647436384E-5 0.0 3.007927694230915E-5 1.0026425647436384E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 395 0.0 36.07473 3 CGCGTAG 290 0.0 23.749912 54-55 TCGCGTA 305 0.0 23.361916 52-53 ACTCGCG 310 0.0 22.985283 50-51 CTCGCGT 350 0.0 21.03685 52-53 CGTACGC 340 0.0 19.557722 20-21 CGTAGCG 365 0.0 19.519497 56-57 ATCAACG 7515 0.0 17.98122 10-11 CAACGCA 7570 0.0 17.944693 12-13 TCAACGC 7470 0.0 17.135786 10-11 ACGTACG 420 0.0 16.96333 20-21 CGCTAGT 435 0.0 16.379004 90-91 AACGCAG 7970 0.0 15.941578 12-13 GTATCAA 10000 0.0 15.009042 9 AGTGGTA 9830 0.0 14.978923 5 ACGCAGA 9005 0.0 14.926976 14-15 GGTATCA 10190 0.0 14.449519 8 AAGCAGT 11435 0.0 13.976578 1 CGACGAC 555 0.0 13.6933 60-61 CGCAGAG 9590 0.0 13.2239685 14-15 >>END_MODULE