FastQCFastQC Report
Sat 18 Jun 2016
SRR578576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10041330
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120866812.036931362678052No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT291480.29028027163732295No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG108340.10789407379301348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2624450.091.4191065
GTATCAA2647000.091.142769
AAGCAGT2651750.090.9914551
GGTATCA2648450.090.939048
TGGTATC2642750.090.904647
GTGGTAT2647550.090.809876
GCAGTGG2659100.090.446783
AGCAGTG2664950.090.388672
CAGTGGT2661350.090.14354
TCAACGC2637800.046.259812-13
CAACGCA2634850.046.20069512-13
ATCAACG2634600.046.19725410-11
AACGCAG2647750.046.1061514-15
ACGCAGA2646200.046.04339214-15
CGCAGAG2653750.046.00543216-17
TATCAAC2657050.045.8203310-11
AGAGTAC2589150.045.71796418-19
CAGAGTA2639200.045.67620518-19
GCAGAGT2667600.045.4665216-17
GAGTACT1540700.045.39426820-21
Produced by FastQC (version 0.11.5)