##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10041330 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.618135944142857 31.0 31.0 33.0 27.0 34.0 2 30.920006712258235 31.0 31.0 34.0 27.0 34.0 3 31.004099954886453 31.0 31.0 34.0 27.0 34.0 4 34.45463509315997 37.0 35.0 37.0 30.0 37.0 5 34.51003452729867 37.0 35.0 37.0 30.0 37.0 6 34.49260964433994 37.0 35.0 37.0 30.0 37.0 7 34.408084984758 37.0 35.0 37.0 30.0 37.0 8 34.35430665061302 37.0 35.0 37.0 30.0 37.0 9 35.77712534096579 39.0 35.0 39.0 30.0 39.0 10-11 35.76523976405516 39.0 35.0 39.0 30.0 39.0 12-13 35.816143230030285 39.0 35.0 39.0 30.0 39.0 14-15 36.83184334146971 39.0 36.0 41.0 30.0 41.0 16-17 36.800647922137806 39.5 36.0 41.0 30.0 41.0 18-19 36.7901560849011 40.0 36.0 41.0 30.0 41.0 20-21 36.68126343820988 39.5 36.0 41.0 30.0 41.0 22-23 36.37694135139469 39.0 36.0 40.5 29.0 41.0 24-25 36.26567670816515 39.0 36.0 40.0 29.0 41.0 26-27 36.19728173459093 39.0 36.0 40.0 29.0 41.0 28-29 35.86402329173526 39.0 35.0 40.5 27.0 41.0 30-31 35.58166238934484 38.5 34.5 40.0 26.5 41.0 32-33 35.24439840140698 38.0 34.0 40.0 25.0 41.0 34-35 35.03349830151982 38.0 34.0 40.0 25.0 41.0 36-37 35.06770821195997 38.0 34.0 40.0 25.0 41.0 38-39 34.402985311706715 37.0 33.5 40.0 25.0 41.0 40-41 34.37085963711978 37.0 33.0 40.0 24.5 41.0 42-43 34.19531934514651 37.0 33.0 40.0 24.0 41.0 44-45 34.0386156017181 37.0 33.0 40.0 23.0 41.0 46-47 33.836063300379536 37.0 33.0 40.0 23.0 41.0 48-49 33.48526709111243 37.0 33.0 40.0 21.0 41.0 50-51 32.22674351903583 36.0 31.5 39.0 16.0 40.5 52-53 31.850289403893704 36.0 31.0 39.0 9.0 40.0 54-55 31.56667692427198 37.0 30.5 40.0 2.0 41.0 56-57 31.090093394002587 37.0 29.5 40.0 2.0 41.0 58-59 30.779555447336158 36.0 29.0 40.0 2.0 41.0 60-61 30.48262949230829 36.0 28.5 39.5 2.0 41.0 62-63 30.151468032621175 35.0 28.0 39.0 2.0 41.0 64-65 29.815745971898146 35.0 28.0 39.0 2.0 41.0 66-67 29.50936708583425 35.0 28.0 39.0 2.0 40.0 68-69 29.1759364048388 35.0 27.5 38.0 2.0 40.0 70-71 28.7782815125088 35.0 26.0 37.5 2.0 40.0 72-73 28.382447942653016 34.5 26.0 37.0 2.0 39.5 74-75 27.981755902853507 34.0 26.0 36.5 2.0 39.0 76-77 27.575404055040515 34.0 25.5 36.0 2.0 39.0 78-79 27.178264333509606 34.0 24.5 35.5 2.0 38.5 80-81 26.776270324747816 34.0 24.0 35.0 2.0 37.0 82-83 26.389778445683987 33.5 22.5 35.0 2.0 37.0 84-85 25.97075880386363 33.0 20.0 35.0 2.0 36.0 86-87 25.59429039778595 33.0 19.5 35.0 2.0 36.0 88-89 25.184220018662867 32.5 18.0 35.0 2.0 35.5 90-91 24.801062658034343 32.0 13.0 35.0 2.0 35.0 92-93 24.419704859814388 32.0 6.5 35.0 2.0 35.0 94-95 23.951275528241776 32.0 2.0 35.0 2.0 35.0 96-97 23.443968129719867 31.0 2.0 34.5 2.0 35.0 98-99 22.813375469185857 31.0 2.0 34.0 2.0 35.0 100-101 21.20997308125517 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4639444483563935 1101 2 0.5076261523760479 1101 3 0.5611166654980302 1101 4 0.6642899641636362 1101 5 0.5923425276551413 1101 6 0.4572364113390748 1101 7 0.48661175168570736 1101 8 0.6256178274233619 1101 9 0.4940021831802426 1101 10-11 0.6442059129241855 1101 12-13 0.5795853918596663 1101 14-15 0.7705308904206518 1101 16-17 0.7047719734044975 1101 18-19 0.6406979438883056 1101 20-21 0.6979603686256795 1101 22-23 0.7550810189131951 1101 24-25 0.9338180117498425 1101 26-27 0.8272645221326798 1101 28-29 0.8115448472845443 1101 30-31 0.9324179821687935 1101 32-33 0.982224937970706 1101 34-35 1.0328267767841908 1101 36-37 0.862758754698099 1101 38-39 1.0582316256977506 1101 40-41 0.9385588750502833 1101 42-43 1.0818915400751763 1101 44-45 0.9940400515291472 1101 46-47 1.004253150699121 1101 48-49 1.054720407430402 1101 50-51 1.1089075912141837 1101 52-53 1.1182992250636339 1101 54-55 0.9983285886421989 1101 56-57 0.8736604145157045 1101 58-59 0.946452600888275 1101 60-61 1.0699742367032634 1101 62-63 1.0990332462784274 1101 64-65 1.1345586449504346 1101 66-67 1.0161168333789767 1101 68-69 1.1282626028497909 1101 70-71 1.0233744114014662 1101 72-73 1.1277124288966363 1101 74-75 1.1696396537906963 1101 76-77 1.0324524381546176 1101 78-79 1.0934713497268227 1101 80-81 1.1410502567437923 1101 82-83 1.1636184503173084 1101 84-85 1.2756979053139688 1101 86-87 1.3138840924830326 1101 88-89 1.3940640864147262 1101 90-91 1.6205016539353743 1101 92-93 1.6536873543486834 1101 94-95 1.6828503317563346 1101 96-97 1.9414693438600104 1101 98-99 2.217229931356293 1101 100-101 2.660823628297816 1102 1 0.5081994557846023 1102 2 0.39236622908759244 1102 3 0.4172387462321616 1102 4 0.5222948237521265 1102 5 0.4910736500812547 1102 6 0.3427400317695657 1102 7 0.47931030590589785 1102 8 0.621299863018784 1102 9 0.5824047762636724 1102 10-11 0.6262285018226095 1102 12-13 0.5832656995414993 1102 14-15 0.777356048006844 1102 16-17 0.7599417473167094 1102 18-19 0.6761765789848297 1102 20-21 0.6634131153444827 1102 22-23 0.7126517772778911 1102 24-25 0.8218279492747129 1102 26-27 0.8313963516959788 1102 28-29 0.7641977910555795 1102 30-31 0.9242551404652843 1102 32-33 1.0239473119918827 1102 34-35 1.0780538029970543 1102 36-37 0.9238200130761101 1102 38-39 0.9496604262568198 1102 40-41 0.9515530682767164 1102 42-43 1.0826646196666943 1102 44-45 1.0127816802629184 1102 46-47 1.0029878785611572 1102 48-49 1.189433308589905 1102 50-51 1.1121977031208488 1102 52-53 1.0652836148739304 1102 54-55 0.9647422008812789 1102 56-57 0.809231240945639 1102 58-59 0.8678191217538078 1102 60-61 1.013865196190629 1102 62-63 1.029091428579374 1102 64-65 1.0224344737373805 1102 66-67 1.0018933886781092 1102 68-69 1.1167037774671158 1102 70-71 0.9819781103091536 1102 72-73 1.038061108245678 1102 74-75 1.1476032631487918 1102 76-77 1.0575465417168886 1102 78-79 1.0063973092360925 1102 80-81 1.130785681769087 1102 82-83 1.2667096567264942 1102 84-85 1.2704419210484943 1102 86-87 1.3385041991567626 1102 88-89 1.466761569112844 1102 90-91 1.477154576141185 1102 92-93 1.4150362389043067 1102 94-95 1.4227623725981715 1102 96-97 1.6698221549712216 1102 98-99 1.7525949109822108 1102 100-101 2.1595878398647983 1103 1 0.4076832756212241 1103 2 0.4304131591281397 1103 3 0.27758195111208295 1103 4 0.4203389845005603 1103 5 0.3808292588344955 1103 6 0.27774711386602036 1103 7 0.5091260481269586 1103 8 0.43174526493164933 1103 9 0.6515300224172478 1103 10-11 0.5542356697967179 1103 12-13 0.4228560649977595 1103 14-15 0.6798760804125479 1103 16-17 0.6323242117364032 1103 18-19 0.6311124384551121 1103 20-21 0.7429953758159797 1103 22-23 0.7832378568696114 1103 24-25 0.6218999711026214 1103 26-27 0.7556971372854377 1103 28-29 0.7314040434809641 1103 30-31 0.6238711096026819 1103 32-33 0.8537574601754301 1103 34-35 0.8233363067623571 1103 36-37 0.7916872733477192 1103 38-39 0.6106650923510486 1103 40-41 0.6994489907779027 1103 42-43 0.5389268945605394 1103 44-45 0.6961970610409622 1103 46-47 0.7979843464116883 1103 48-49 0.8199879339915341 1103 50-51 0.7147393239027338 1103 52-53 0.6074644749401479 1103 54-55 0.7061033002741333 1103 56-57 0.7273756205809256 1103 58-59 0.8153826448973653 1103 60-61 0.7956191337766825 1103 62-63 0.7778099612284883 1103 64-65 0.7641372196979006 1103 66-67 0.7723839068969305 1103 68-69 0.8467232374487814 1103 70-71 0.9507789257047499 1103 72-73 0.9519971408576069 1103 74-75 1.0843099650267973 1103 76-77 1.1341325731242051 1103 78-79 1.202187680792214 1103 80-81 1.127259147877819 1103 82-83 1.273699407535542 1103 84-85 1.1975462121173948 1103 86-87 1.3217679995030522 1103 88-89 1.4444665616433277 1103 90-91 1.2305428842880524 1103 92-93 1.3631788086267917 1103 94-95 1.4433067423268078 1103 96-97 1.625635056860009 1103 98-99 1.7013811135003856 1103 100-101 1.9418736091167084 1104 1 0.20055994353551299 1104 2 0.2810389307649679 1104 3 0.26914626160273514 1104 4 0.2793309583133663 1104 5 0.14787577503792448 1104 6 0.1416782182300267 1104 7 0.3687934679247462 1104 8 0.31131622175840334 1104 9 0.38551879471940254 1104 10-11 0.4756708531763394 1104 12-13 0.3501763707536725 1104 14-15 0.4184005201627343 1104 16-17 0.589144582686707 1104 18-19 0.6957209424532209 1104 20-21 0.5595414122293789 1104 22-23 0.44645931001783623 1104 24-25 0.5527306787121518 1104 26-27 0.6228216757585727 1104 28-29 0.7720739610391476 1104 30-31 0.618551272140472 1104 32-33 0.8035246303136034 1104 34-35 0.7494847140981236 1104 36-37 0.8294325927391455 1104 38-39 0.5557928551819771 1104 40-41 0.6414352894765116 1104 42-43 0.7083186046270882 1104 44-45 0.9001387282072599 1104 46-47 0.728379321145944 1104 48-49 0.7026626280580857 1104 50-51 0.6576687039622158 1104 52-53 0.47478734335980377 1104 54-55 0.5540085882383785 1104 56-57 0.5282171740353867 1104 58-59 0.5941224058842245 1104 60-61 0.48463877810049283 1104 62-63 0.4237348529155618 1104 64-65 0.6147242639701354 1104 66-67 0.45213503237344455 1104 68-69 0.5500859768999504 1104 70-71 0.6754640716849067 1104 72-73 0.5011156631101414 1104 74-75 0.6651268806739736 1104 76-77 0.7223799585086716 1104 78-79 0.7402915875665528 1104 80-81 0.6448025712764398 1104 82-83 0.696756883674098 1104 84-85 0.7109587772608634 1104 86-87 0.7835202612248011 1104 88-89 0.9321846027088121 1104 90-91 1.025599599049169 1104 92-93 0.9005615387470094 1104 94-95 0.9995670344559251 1104 96-97 1.2296919202003274 1104 98-99 1.440187959232393 1104 100-101 1.6962138934632414 1105 1 -0.0393205785924593 1105 2 0.10293021772447375 1105 3 0.10635793196735577 1105 4 0.13601239042613855 1105 5 0.16799005033321635 1105 6 0.1528346432135521 1105 7 0.3296332649122391 1105 8 0.3099255315884335 1105 9 0.24628349797885107 1105 10-11 0.30762754344141996 1105 12-13 0.2405564611161921 1105 14-15 0.14594751535127415 1105 16-17 0.34933672156363826 1105 18-19 0.14390914727444937 1105 20-21 0.1715746952424695 1105 22-23 0.28003298880978633 1105 24-25 0.5166713982446254 1105 26-27 0.4678338778570321 1105 28-29 0.6768656415270797 1105 30-31 0.5196009989527965 1105 32-33 0.5879879883900685 1105 34-35 0.5505966564724432 1105 36-37 0.79399309659631 1105 38-39 0.6110550250488913 1105 40-41 0.522564360642761 1105 42-43 0.8103445020264033 1105 44-45 0.8128764566592039 1105 46-47 0.6105547342698472 1105 48-49 0.7463822086302443 1105 50-51 0.7511311584351503 1105 52-53 0.6717982926555344 1105 54-55 0.7410659283810936 1105 56-57 0.826362499514051 1105 58-59 0.8515312131839288 1105 60-61 0.7929820600338999 1105 62-63 0.8357674665458639 1105 64-65 0.9286536943302615 1105 66-67 0.7902577543942577 1105 68-69 0.8425119955578246 1105 70-71 0.8562085178493284 1105 72-73 0.7098471572505751 1105 74-75 0.6131673786398437 1105 76-77 0.5277241300256073 1105 78-79 0.7250943669812031 1105 80-81 0.7561124150011231 1105 82-83 0.9278270184409969 1105 84-85 0.9621876999235006 1105 86-87 0.9471625868929543 1105 88-89 0.8843121749273557 1105 90-91 1.0581083051808342 1105 92-93 1.2250226684382355 1105 94-95 1.3055012203787726 1105 96-97 1.45712098748578 1105 98-99 1.7593774645894023 1105 100-101 2.1077855769705316 1106 1 0.23043891479118628 1106 2 0.22061911230808562 1106 3 0.21317046735159906 1106 4 -0.0175676801132596 1106 5 0.24772448910751166 1106 6 0.22687110779129682 1106 7 0.3743721366085637 1106 8 0.3174622126812494 1106 9 0.3479809732368011 1106 10-11 0.23224233577845865 1106 12-13 0.3587807580456541 1106 14-15 0.2534515791642491 1106 16-17 0.4048121318546336 1106 18-19 0.44723380175730654 1106 20-21 0.33379443794798647 1106 22-23 0.44924864435974854 1106 24-25 0.6396728118437665 1106 26-27 0.4721976212956349 1106 28-29 0.5152188173175176 1106 30-31 0.5656957502040072 1106 32-33 0.519768957091209 1106 34-35 0.5049215522221786 1106 36-37 0.6390487046907367 1106 38-39 0.6309157750004317 1106 40-41 0.47346062986985515 1106 42-43 0.7007543081066601 1106 44-45 0.66881308524205 1106 46-47 0.7303176721293028 1106 48-49 0.7537689064241988 1106 50-51 0.6035839838411832 1106 52-53 0.6584579182463379 1106 54-55 0.793938618495563 1106 56-57 0.7413885053515763 1106 58-59 0.8397256585317265 1106 60-61 0.6604613953470917 1106 62-63 0.7927306925524782 1106 64-65 0.9043422669958545 1106 66-67 0.8952609779105103 1106 68-69 0.8280738740255167 1106 70-71 0.9620090792492029 1106 72-73 0.9478819263476623 1106 74-75 0.8464336128978758 1106 76-77 0.8998099887658562 1106 78-79 0.9931754756149651 1106 80-81 1.0286433428346875 1106 82-83 1.1686271559887729 1106 84-85 1.2346917575936942 1106 86-87 1.2356760857331217 1106 88-89 1.2535726511203116 1106 90-91 1.0902270574455386 1106 92-93 1.243602385948222 1106 94-95 1.260818925530053 1106 96-97 1.5142040230747575 1106 98-99 1.591426612787611 1106 100-101 1.8824374184254218 1107 1 0.11245774543993292 1107 2 0.13564869315706574 1107 3 0.1844847327249184 1107 4 0.10098635566141212 1107 5 0.1847126710065936 1107 6 0.217262511190782 1107 7 0.24937596325268174 1107 8 0.24269345767546469 1107 9 0.19309778377342468 1107 10-11 0.1938351763942805 1107 12-13 0.2232666573070219 1107 14-15 0.24790943713345825 1107 16-17 0.22361053000360442 1107 18-19 0.23295301062088214 1107 20-21 0.22935255844278402 1107 22-23 0.4206742962241137 1107 24-25 0.39832695885809244 1107 26-27 0.3734458370115732 1107 28-29 0.44593535642937354 1107 30-31 0.4969024112345366 1107 32-33 0.47178460183625504 1107 34-35 0.3705371079754869 1107 36-37 0.33659918969563307 1107 38-39 0.39848036519354224 1107 40-41 0.4087223945757401 1107 42-43 0.42034334432629095 1107 44-45 0.413297787564737 1107 46-47 0.4774122213862242 1107 48-49 0.5484469521659463 1107 50-51 0.4966331473189811 1107 52-53 0.5370452458248813 1107 54-55 0.6679815792502133 1107 56-57 0.4865980319156016 1107 58-59 0.5072620917434385 1107 60-61 0.5169262881120602 1107 62-63 0.5335459501835196 1107 64-65 0.6571587438135076 1107 66-67 0.6978892505811523 1107 68-69 0.6604192019600212 1107 70-71 0.5743853852027492 1107 72-73 0.5899309162915962 1107 74-75 0.6000416310521928 1107 76-77 0.6675397342495266 1107 78-79 0.7091882058810519 1107 80-81 0.73642227849367 1107 82-83 0.8024372343014896 1107 84-85 0.9323618697232661 1107 86-87 0.8968250712274717 1107 88-89 0.9336567705472056 1107 90-91 1.0117261719778874 1107 92-93 1.1277290745661794 1107 94-95 1.0894800895418015 1107 96-97 1.1552832835535334 1107 98-99 1.350619155281155 1107 100-101 1.6248920415454684 1108 1 0.20590047859877103 1108 2 0.1840139225275017 1108 3 -0.029620350619893543 1108 4 0.2110821815327455 1108 5 0.24296884954358688 1108 6 0.26032320825649435 1108 7 0.1998572967697001 1108 8 0.15788397978594304 1108 9 0.07675073872589877 1108 10-11 0.09269727861221355 1108 12-13 0.23073909152660832 1108 14-15 0.11477616808813451 1108 16-17 0.3044146077002594 1108 18-19 0.09873672738627448 1108 20-21 0.11052473091832837 1108 22-23 0.2461541694849032 1108 24-25 0.3176115986156418 1108 26-27 0.2697954168435075 1108 28-29 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26-27 0.2174040583168093 2311 28-29 0.196854312718159 2311 30-31 0.08545892038672065 2311 32-33 0.11980542420982232 2311 34-35 0.029410154797751886 2311 36-37 0.1327633792315126 2311 38-39 0.02679729822513366 2311 40-41 0.17314098520912324 2311 42-43 0.2123695073210996 2311 44-45 0.17856226606020442 2311 46-47 0.14703240858882083 2311 48-49 0.24860659180364308 2311 50-51 0.36695519844592184 2311 52-53 0.3461563586163372 2311 54-55 0.08574955820747121 2311 56-57 0.04954996092336472 2311 58-59 0.05482275219162247 2311 60-61 0.20883392906762666 2311 62-63 0.06876850955525882 2311 64-65 0.17788238502832954 2311 66-67 0.13260167692346414 2311 68-69 0.01681569123610771 2311 70-71 0.1164999601080936 2311 72-73 0.01563345535515026 2311 74-75 -0.0025067483436416182 2311 76-77 0.19909097515539997 2311 78-79 0.14898678322769854 2311 80-81 0.11258950561590098 2311 82-83 0.048566603937509 2311 84-85 0.1148027583144895 2311 86-87 0.10969865600064921 2311 88-89 0.07464666506345097 2311 90-91 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44-45 -2.3299059365148196 2315 46-47 -2.9340836736624425 2315 48-49 -4.20147523714143 2315 50-51 -3.350574977945513 2315 52-53 -2.9096408311958584 2315 54-55 -2.47216851244114 2315 56-57 -2.362423042989178 2315 58-59 -2.6532029667979664 2315 60-61 -2.569733607500858 2315 62-63 -2.215973812829599 2315 64-65 -2.100087694330096 2315 66-67 -1.858093370380903 2315 68-69 -1.7617602873896772 2315 70-71 -1.8631281710111018 2315 72-73 -1.8305859408109555 2315 74-75 -1.9845933809382146 2315 76-77 -2.0167865176901003 2315 78-79 -1.9099673308660208 2315 80-81 -1.930856152878878 2315 82-83 -1.9282564037292609 2315 84-85 -1.775182438359682 2315 86-87 -1.77289319651128 2315 88-89 -2.059804648552813 2315 90-91 -2.071333441507793 2315 92-93 -1.924686668133777 2315 94-95 -2.1384866194439525 2315 96-97 -2.136549245717166 2315 98-99 -2.326722529018461 2315 100-101 -2.3770274393404094 2316 1 -1.3485581961133768 2316 2 -1.2548079835991928 2316 3 -2.432141716590081 2316 4 -1.5558486831774303 2316 5 -1.377274963774532 2316 6 -2.809282798365153 2316 7 -7.169340008690959 2316 8 -8.029602276737336 2316 9 -4.684747935444207 2316 10-11 -2.471598250541284 2316 12-13 -2.461347971383887 2316 14-15 -2.5341216193560925 2316 16-17 -2.4165705039971 2316 18-19 -1.8941556773676425 2316 20-21 -2.891330961487199 2316 22-23 -3.564431659065626 2316 24-25 -2.3138961499514537 2316 26-27 -2.693077372693878 2316 28-29 -3.489529336081233 2316 30-31 -3.9390415976151303 2316 32-33 -3.94166950511341 2316 34-35 -3.1592007814766276 2316 36-37 -4.2116184196791835 2316 38-39 -3.6870421564747673 2316 40-41 -4.464317061801978 2316 42-43 -3.773060470268362 2316 44-45 -3.3562936496549085 2316 46-47 -3.098759992124105 2316 48-49 -3.2372836047065228 2316 50-51 -2.6914989752634675 2316 52-53 -3.102356298004061 2316 54-55 -4.664927480715999 2316 56-57 -3.364888502735454 2316 58-59 -2.869777471608252 2316 60-61 -3.5611513895802176 2316 62-63 -3.3721226395834485 2316 64-65 -4.876426464603398 2316 66-67 -3.6765956770290735 2316 68-69 -3.618981218642194 2316 70-71 -3.988880860861702 2316 72-73 -3.672569940715807 2316 74-75 -3.3171163768006693 2316 76-77 -3.9113437200373333 2316 78-79 -5.1308549629798 2316 80-81 -4.01315621487516 2316 82-83 -3.693217949681589 2316 84-85 -3.8857074591366114 2316 86-87 -4.9306895781288596 2316 88-89 -5.330452561716392 2316 90-91 -4.8018623574946595 2316 92-93 -4.883720403327892 2316 94-95 -5.047632245286735 2316 96-97 -5.180383098659615 2316 98-99 -5.284719677486915 2316 100-101 -5.427457889772375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83290.0 3 16827.0 4 15062.0 5 14981.0 6 17297.0 7 21903.0 8 27478.0 9 32671.0 10 38370.0 11 45888.0 12 48176.0 13 54774.0 14 61522.0 15 73157.0 16 91873.0 17 120562.0 18 170391.0 19 243771.0 20 313105.0 21 230431.0 22 144166.0 23 130423.0 24 137538.0 25 147546.0 26 163205.0 27 184654.0 28 213568.0 29 247981.0 30 291053.0 31 346016.0 32 418117.0 33 515820.0 34 654188.0 35 891073.0 36 1249363.0 37 1376836.0 38 1035215.0 39 172885.0 40 154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.395616584121495 46.909940278720576 25.205060076240947 17.489383060916982 2 16.640798643962626 48.64225780781269 18.98913921894656 15.727804329278127 3 42.63754293730655 21.38411453244892 19.847303892052 16.13103863819253 4 16.48595230600203 19.821645412427664 20.86132448860341 42.831077792966894 5 16.523652832409596 46.10936877432763 21.809866414422423 15.557111978840352 6 42.84906199003321 20.862493516083106 19.720686216697786 16.567758277185902 7 17.38155734857367 20.298880729915496 46.83856367466613 15.480998246844704 8 42.41362337066323 21.026802642265807 20.570891375953497 15.988682611117468 9 42.03756949163828 20.656681653290477 21.220648647765035 16.085100207306212 10-11 16.22239133328347 33.6239560795839 33.72326328623832 16.43038930089431 12-13 16.061278418932122 21.0377930489045 33.66749774673591 29.233430785427473 14-15 16.259860322213907 46.75390623092332 20.57184611463912 16.414387332223654 16-17 29.213189383664595 20.829187004667258 20.693345379797726 29.26427823187042 18-19 16.364411451339222 33.67288715313693 20.695319528365474 29.267381867158377 20-21 29.332517508334536 33.6873778666432 20.72915762597915 16.250946999043112 22-23 29.27728872949997 20.768649116459937 33.621526352062745 16.332535801977343 24-25 16.28223629595001 33.50451066031077 21.148129040434537 29.065124003304682 26-27 16.554939832499418 25.693908924796105 41.208291737156 16.54285950554848 28-29 26.70849432726634 20.5976652079094 36.25534467642604 16.438495788398214 30-31 24.61411760744299 21.68589158800145 36.67456066846398 17.025430136091583 32-33 19.490209453255027 23.857625072535853 37.91012150153999 18.74204397266913 34-35 19.04517032547062 22.966434539147844 38.57053266795946 19.417862467422072 36-37 18.818732280827284 23.04379361711975 38.703832006885825 19.43364209516714 38-39 19.054215249512048 23.151368335749865 38.510011654452484 19.284404760285604 40-41 19.12015859648172 22.891996401766594 38.66816041712086 19.319684584630824 42-43 19.35515176007532 23.14441967987598 38.60808179810196 18.892346761946737 44-45 18.92910264791805 23.435747887557525 38.296568276405466 19.33858118811896 46-47 19.267110266491184 23.19159723286729 38.19822522540879 19.34306727523274 48-49 19.287951631066573 23.212878455195142 38.43831194953076 19.06085796420753 50-51 19.532244693114322 23.313012932690295 37.67190750568514 19.482834868510235 52-53 19.703454982545026 24.178879901594364 36.66497672296419 19.452688392896416 54-55 20.288329093101932 24.912489494903504 35.25994602858781 19.539235383406755 56-57 20.490756671585768 25.40894438131241 34.12250340013782 19.977795546963996 58-59 20.5159451416902 26.21062935050696 33.340673228466486 19.93275227933635 60-61 20.832609128512715 26.359316880695033 32.79581020797269 20.012263782819556 62-63 20.843345642378722 26.27156490945578 32.934491698867475 19.950597749298026 64-65 20.49721884991594 26.04572423862308 32.98662055852566 20.47043635293532 66-67 20.40267384495581 25.80165931182955 33.54494182086717 20.250725022347467 68-69 20.41471109476293 25.49993364638425 33.73274357655155 20.352611682301276 70-71 20.232132037551075 25.561426322319942 34.06597632985754 20.140465310271445 72-73 19.91971150541052 25.259561439603544 34.04213738663117 20.778589668354766 74-75 20.22380050667019 25.575670664475442 33.402192243043075 20.798336585811292 76-77 20.69400607971659 26.09265253751677 33.0707769454971 20.14256443726954 78-79 20.658193397322158 25.79482669628331 32.90929125562729 20.637688650767252 80-81 20.422706697846163 26.139145978034193 32.83407778201785 20.604069542101787 82-83 21.23740517376605 26.35405045148423 32.109848618249686 20.298695756500035 84-85 20.969451502017638 26.66295023165446 31.67490160912669 20.692696657201214 86-87 20.738697477430026 26.923288847518783 31.77737105146921 20.560642623581977 88-89 20.70170834443633 26.65007456879152 31.708955692682228 20.93926139408992 90-91 20.569385774486783 26.57585937103813 31.83963182914073 21.01512302533436 92-93 20.565739200338815 27.135512228883613 31.72146749492534 20.577281075852238 94-95 20.587050851132172 26.617099319875372 31.401126075323027 21.394723753669428 96-97 20.533558777944894 26.44997558336433 31.885107725080836 21.13135791360994 98-99 21.061190793753752 26.60341909657481 31.353113498997498 20.982276610673942 100-101 21.395173218488374 26.385811907858063 31.109992797535952 21.109022076117608 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11803.0 1 7280.5 2 2019.5 3 1146.5 4 942.0 5 816.5 6 935.0 7 1163.0 8 1091.5 9 1083.0 10 1540.0 11 10765.0 12 198296.0 13 221135.0 14 57543.5 15 50689.0 16 53529.5 17 57679.0 18 61309.5 19 63163.0 20 64760.0 21 66447.0 22 68870.5 23 73960.5 24 82552.5 25 93867.0 26 103586.0 27 111880.0 28 122148.5 29 134337.0 30 144801.5 31 154243.5 32 165843.5 33 180211.0 34 199463.5 35 214619.0 36 225638.0 37 240879.5 38 254989.5 39 268117.0 40 284785.0 41 296218.0 42 299735.0 43 308095.0 44 321639.0 45 329038.0 46 327367.0 47 327228.0 48 333088.0 49 329078.0 50 314931.0 51 302336.0 52 294402.0 53 292077.5 54 274402.0 55 249018.5 56 242456.0 57 229449.5 58 219720.5 59 188564.5 60 134473.5 61 102534.5 62 77046.5 63 57054.5 64 41547.0 65 28933.0 66 19343.5 67 12544.0 68 7857.5 69 4791.5 70 3259.0 71 2448.0 72 1959.0 73 1675.0 74 1461.5 75 1295.5 76 1212.5 77 1121.0 78 1023.0 79 930.5 80 849.5 81 800.5 82 772.0 83 666.0 84 552.5 85 466.0 86 393.5 87 352.5 88 279.5 89 205.5 90 163.5 91 137.0 92 93.5 93 67.5 94 52.5 95 29.5 96 21.0 97 18.5 98 14.0 99 9.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12024303553413741 2 0.10883020476371158 3 0.10383086702657914 4 0.09253754233751904 5 0.09742733283339956 6 0.08777721676311803 7 0.09663062562429478 8 0.09295581362229904 9 0.08791664052471136 10-11 0.07786319142982055 12-13 0.06969196311643974 14-15 0.07776858244873935 16-17 0.06822303419965284 18-19 0.06076884237446633 20-21 0.05657617068655248 22-23 0.04922654668256097 24-25 0.04642811261058047 26-27 0.04332095449507187 28-29 0.04571605554244308 30-31 0.0373556092668999 32-33 0.0489377403192605 34-35 0.05944929605938656 36-37 0.05040666923604741 38-39 0.05229884885767125 40-41 0.05272209956250815 42-43 0.04793687688782263 44-45 0.04764807052452215 46-47 0.047120251998490244 48-49 0.04703062243746595 50-51 0.04844477773362692 52-53 0.044879512972883076 54-55 0.04516333991612665 56-57 0.04585547930403642 58-59 0.048360127592659535 60-61 0.04441144748753402 62-63 0.05327979460888149 64-65 0.0490423081404555 66-67 0.04379897882053473 68-69 0.041807210797772804 70-71 0.04952531188597526 72-73 0.04381889650076235 74-75 0.0460446972661988 76-77 0.041672766456236375 78-79 0.04610445030688166 80-81 0.04800160934856239 82-83 0.038097542855378724 84-85 0.04810119774970049 86-87 0.05076020806008766 88-89 0.04605465610631261 90-91 0.042335029323804715 92-93 0.03950173931142588 94-95 0.04328111913461663 96-97 0.03079273363189936 98-99 0.0319728561853858 100-101 0.023946031053655243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.004133E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.578935932329536 #Duplication Level Percentage of deduplicated Percentage of total 1 91.26359681662525 45.247520195254175 2 5.365743319112735 5.32055688495231 3 1.2964977298592224 1.9283693365530319 4 0.5579809542961424 1.1065640793803415 5 0.2933223490308153 0.7271304975059597 6 0.20217691452183462 0.6014229775244462 7 0.12813139378082733 0.4446832714225808 8 0.1099062258540343 0.4359226984145046 9 0.08501845828144322 0.37936122265809946 >10 0.6587072021174322 6.280599092533806 >50 0.01872214799919022 0.6251250409883347 >100 0.013072519561567493 1.2980536630945334 >500 0.0031077716939615133 1.0711879598760048 >1k 0.0034424547990039036 3.3536581686672124 >5k 4.3030684987548795E-4 1.5810742819817667 >10k+ 1.4343561662516263E-4 29.59877062919284 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1208668 12.036931362678052 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 29148 0.29028027163732295 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10834 0.10789407379301348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.958840113809625E-6 0.0 4 0.0 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 5 0.0 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 6 0.0 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 7 0.0 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 8 0.0 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 9 9.958840113809625E-6 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 10-11 9.958840113809625E-6 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 12-13 9.958840113809625E-6 9.958840113809625E-6 0.0 9.958840113809625E-6 0.0 14-15 9.958840113809625E-6 9.958840113809625E-6 0.0 1.991768022761925E-5 0.0 16-17 9.958840113809625E-6 9.958840113809625E-6 0.0 2.489710028452406E-5 0.0 18-19 9.958840113809625E-6 9.958840113809625E-6 0.0 3.4855940398333685E-5 0.0 20-21 9.958840113809625E-6 9.958840113809625E-6 0.0 3.98353604552385E-5 0.0 22-23 1.4938260170714438E-5 9.958840113809625E-6 0.0 3.98353604552385E-5 0.0 24-25 1.991768022761925E-5 9.958840113809625E-6 0.0 4.4814780512143315E-5 0.0 26-27 1.991768022761925E-5 9.958840113809625E-6 0.0 4.979420056904812E-5 0.0 28-29 2.489710028452406E-5 9.958840113809625E-6 0.0 5.9753040682857747E-5 0.0 30-31 2.9876520341428873E-5 9.958840113809625E-6 0.0 6.473246073976256E-5 0.0 32-33 4.4814780512143315E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 34-35 4.979420056904812E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 36-37 4.979420056904812E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 38-39 4.979420056904812E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 40-41 4.979420056904812E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 42-43 4.979420056904812E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 44-45 6.971188079666737E-5 9.958840113809625E-6 0.0 6.971188079666737E-5 0.0 46-47 7.9670720910477E-5 1.991768022761925E-5 0.0 6.971188079666737E-5 0.0 48-49 9.958840113809625E-5 1.991768022761925E-5 0.0 6.971188079666737E-5 0.0 50-51 1.244855014226203E-4 1.991768022761925E-5 0.0 7.469130085357219E-5 0.0 52-53 1.3942376159333474E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 54-55 2.1411506244690693E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 56-57 2.1909448250381175E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 58-59 2.439915827883358E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 60-61 2.738681031297647E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 62-63 3.2864172375571764E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 64-65 3.634976641540513E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 0.0 66-67 4.132918647230994E-4 1.991768022761925E-5 0.0 7.9670720910477E-5 9.958840113809625E-6 68-69 4.929625856335765E-4 1.991768022761925E-5 0.0 8.465014096738181E-5 9.958840113809625E-6 70-71 5.377773661457198E-4 1.991768022761925E-5 0.0 8.962956102428663E-5 9.958840113809625E-6 72-73 6.074892469423871E-4 1.991768022761925E-5 0.0 9.958840113809625E-5 9.958840113809625E-6 74-75 6.722217076821497E-4 1.991768022761925E-5 0.0 9.958840113809625E-5 1.4938260170714438E-5 76-77 7.618512687064362E-4 1.991768022761925E-5 0.0 1.244855014226203E-4 1.991768022761925E-5 78-79 8.913161901859615E-4 1.991768022761925E-5 0.0 1.4938260170714438E-4 1.991768022761925E-5 80-81 0.0010158016916085818 1.991768022761925E-5 0.0 1.4938260170714438E-4 1.991768022761925E-5 82-83 0.0011452666130881068 1.991768022761925E-5 0.0 1.59341441820954E-4 1.991768022761925E-5 84-85 0.0013095874749659657 1.991768022761925E-5 4.9794200569048125E-6 1.6930028193476362E-4 1.991768022761925E-5 86-87 0.0016083526783802543 1.991768022761925E-5 9.958840113809625E-6 1.6930028193476362E-4 1.991768022761925E-5 88-89 0.0020017268628757345 1.991768022761925E-5 9.958840113809625E-6 1.6930028193476362E-4 1.991768022761925E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 262445 0.0 91.419106 5 GTATCAA 264700 0.0 91.14276 9 AAGCAGT 265175 0.0 90.991455 1 GGTATCA 264845 0.0 90.93904 8 TGGTATC 264275 0.0 90.90464 7 GTGGTAT 264755 0.0 90.80987 6 GCAGTGG 265910 0.0 90.44678 3 AGCAGTG 266495 0.0 90.38867 2 CAGTGGT 266135 0.0 90.1435 4 TCAACGC 263780 0.0 46.2598 12-13 CAACGCA 263485 0.0 46.200695 12-13 ATCAACG 263460 0.0 46.197254 10-11 AACGCAG 264775 0.0 46.10615 14-15 ACGCAGA 264620 0.0 46.043392 14-15 CGCAGAG 265375 0.0 46.005432 16-17 TATCAAC 265705 0.0 45.82033 10-11 AGAGTAC 258915 0.0 45.717964 18-19 CAGAGTA 263920 0.0 45.676205 18-19 GCAGAGT 266760 0.0 45.46652 16-17 GAGTACT 154070 0.0 45.394268 20-21 >>END_MODULE