FastQCFastQC Report
Sat 18 Jun 2016
SRR578576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10041330
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139970.13939388507299333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4150.036.6081773
ACTCGCG3250.023.3772250-51
CTCGCGT3200.023.0001952-53
CGCGTAG3250.022.6435854-55
TCGCGTA3150.022.61155752-53
CGTAGCG3500.020.346756-57
CGTACGC3650.020.16093620-21
ACGTACG3900.018.86856820-21
ATCAACG74300.018.24238410-11
CAACGCA76550.017.58211512-13
CGCTAGT4250.016.756990-91
GTATCAA98350.016.5571339
GGTATCA100950.016.083678
AGTGGTA99800.016.0313935
TCAACGC74800.015.61341110-11
AACGCAG78000.014.94239212-13
ACGCAGA90150.014.92968314-15
AAGCAGT121050.014.1810711
TGGTATC115000.014.1599497
GTGGTAT120800.013.3622486
Produced by FastQC (version 0.11.5)