##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10041330 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40575083181212 31.0 31.0 34.0 30.0 34.0 2 31.78252104053945 31.0 31.0 34.0 30.0 34.0 3 31.858042908658515 31.0 31.0 34.0 30.0 34.0 4 35.55768608341724 37.0 35.0 37.0 33.0 37.0 5 35.37898804242067 37.0 35.0 37.0 33.0 37.0 6 35.37586365551177 37.0 35.0 37.0 33.0 37.0 7 35.36647207093084 37.0 35.0 37.0 32.0 37.0 8 35.323838375992025 37.0 35.0 37.0 32.0 37.0 9 36.8670109437694 39.0 37.0 39.0 32.0 39.0 10-11 36.86307834719106 39.0 37.0 39.0 32.5 39.0 12-13 36.853994540563846 39.0 37.0 39.0 32.0 39.0 14-15 38.02883621990314 40.0 37.0 41.0 33.0 41.0 16-17 37.961880099548566 40.0 37.0 41.0 33.0 41.0 18-19 37.896381704415646 40.0 37.0 41.0 32.5 41.0 20-21 37.85503623523975 40.0 37.0 41.0 32.0 41.0 22-23 37.65261434491248 39.5 37.0 41.0 32.0 41.0 24-25 37.658058942391094 39.0 36.5 41.0 32.0 41.0 26-27 37.48962104621599 39.0 36.0 41.0 32.0 41.0 28-29 37.33975484323292 39.0 36.0 41.0 31.5 41.0 30-31 37.203236473654385 39.0 36.0 41.0 31.0 41.0 32-33 37.06525938297018 39.0 36.0 40.5 31.0 41.0 34-35 36.91646619521518 39.0 36.0 40.0 30.0 41.0 36-37 36.765213622099864 39.0 35.0 40.0 30.0 41.0 38-39 36.599146826167456 38.0 35.0 40.0 30.0 41.0 40-41 36.43784299490207 38.0 35.0 40.0 30.0 41.0 42-43 36.52721462196741 39.0 35.0 40.0 30.0 41.0 44-45 36.46449653581746 38.5 35.0 40.0 30.0 41.0 46-47 36.31891656782518 38.0 35.0 40.0 30.0 41.0 48-49 36.17135907295149 38.0 35.0 40.0 29.5 41.0 50-51 35.987387726526265 38.0 35.0 40.0 29.0 41.0 52-53 36.03572191134043 38.0 35.0 40.0 29.0 41.0 54-55 36.02835371409962 38.0 35.0 40.0 29.0 41.0 56-57 35.87821717840167 38.0 34.5 40.0 28.0 41.0 58-59 35.65762732626057 38.0 34.0 40.0 28.0 41.0 60-61 35.40629936472559 38.0 34.0 40.0 28.0 41.0 62-63 35.13175983659535 37.5 34.0 40.0 28.0 41.0 64-65 34.81559146049378 37.0 33.5 40.0 27.0 41.0 66-67 34.48110330006085 36.5 33.0 39.0 26.0 41.0 68-69 34.10808732508542 36.0 33.0 39.0 26.0 41.0 70-71 33.712212426043166 35.5 32.0 39.0 26.0 40.0 72-73 33.32331663235846 35.0 32.0 38.0 26.0 40.0 74-75 32.91071660825807 35.0 32.0 37.5 25.5 39.5 76-77 31.531647301702066 34.0 30.5 36.0 23.0 39.0 78-79 32.1863053997827 35.0 31.0 36.0 25.0 39.0 80-81 32.05691910334587 35.0 31.0 36.0 25.0 38.5 82-83 31.681168879022998 34.5 31.0 36.0 24.0 37.0 84-85 31.394150027934547 34.0 31.0 35.0 23.5 37.0 86-87 31.071247434353815 34.0 31.0 35.0 22.5 36.5 88-89 30.756549281818245 34.0 31.0 35.0 20.5 36.0 90-91 30.408226350493408 34.0 30.0 35.0 20.0 36.0 92-93 30.03148313022279 34.0 30.0 35.0 18.0 35.5 94-95 29.648738513722783 34.0 30.0 35.0 13.5 35.0 96-97 29.176776134237198 34.0 29.0 35.0 4.5 35.0 98-99 28.355449477310277 34.0 29.0 35.0 2.0 35.0 100-101 27.2906459602463 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3633590857324016 1101 2 0.2613742742598113 1101 3 0.36578625309309487 1101 4 0.29263718109633174 1101 5 0.2679967237217653 1101 6 0.19424008439321483 1101 7 0.2502311067720058 1101 8 0.1630314223731446 1101 9 0.29957189640599324 1101 10-11 0.2888000960816086 1101 12-13 0.2951968607269322 1101 14-15 0.2066560623170801 1101 16-17 0.2779239280187511 1101 18-19 0.3829683636583354 1101 20-21 0.314368619053063 1101 22-23 0.5050027233655143 1101 24-25 0.45347903556046276 1101 26-27 0.3873271974462469 1101 28-29 0.3936605143348757 1101 30-31 0.35951839067051594 1101 32-33 0.42346819172986017 1101 34-35 0.4147219674607143 1101 36-37 0.4526043797397321 1101 38-39 0.3644571074865368 1101 40-41 0.3984383486642926 1101 42-43 0.3330349889856947 1101 44-45 0.3080537613094876 1101 46-47 0.36212113570816484 1101 48-49 0.28023667096869076 1101 50-51 0.4014910967647367 1101 52-53 0.41469648239422696 1101 54-55 0.4546708010207041 1101 56-57 0.5042050240929328 1101 58-59 0.48266614213718384 1101 60-61 0.43968192631457015 1101 62-63 0.36606666996112835 1101 64-65 0.3686801313362835 1101 66-67 0.46035419388207544 1101 68-69 0.3852139133078083 1101 70-71 0.4261633263657032 1101 72-73 0.5590174646527117 1101 74-75 0.515145472819448 1101 76-77 0.40479136707593 1101 78-79 0.4542065154078401 1101 80-81 0.3709043705567723 1101 82-83 0.5368873042144244 1101 84-85 0.5092484319200246 1101 86-87 0.5264781644178882 1101 88-89 0.5571938011225264 1101 90-91 0.5096311665131488 1101 92-93 0.5945342443578632 1101 94-95 0.4916134714190825 1101 96-97 0.5055805955630959 1101 98-99 0.8336463741703213 1101 100-101 1.0069390441458204 1102 1 0.24953338463517127 1102 2 0.271283023035398 1102 3 0.28273978778497266 1102 4 0.27695198484774153 1102 5 0.24421095535451087 1102 6 0.15079801852560593 1102 7 0.2482060648293256 1102 8 0.10823412031746926 1102 9 0.2222835481303136 1102 10-11 0.2543714515334514 1102 12-13 0.3421733320564755 1102 14-15 0.31584264564763487 1102 16-17 0.21697993054726794 1102 18-19 0.2441614057735393 1102 20-21 0.27070817159487603 1102 22-23 0.40226936489521137 1102 24-25 0.3477892533321665 1102 26-27 0.41967489894715726 1102 28-29 0.44742797584852667 1102 30-31 0.4328275844310241 1102 32-33 0.3863998289583108 1102 34-35 0.313126646305669 1102 36-37 0.3697151046985425 1102 38-39 0.3195176400599635 1102 40-41 0.2850787933661678 1102 42-43 0.30800948979288023 1102 44-45 0.3268069139599348 1102 46-47 0.3227229874196169 1102 48-49 0.23516245458660734 1102 50-51 0.36127036995603845 1102 52-53 0.4056594741108128 1102 54-55 0.4216827808397099 1102 56-57 0.466033229451817 1102 58-59 0.5245916673093589 1102 60-61 0.49362130309980756 1102 62-63 0.29711021452919795 1102 64-65 0.2935960185380324 1102 66-67 0.3208718401321988 1102 68-69 0.3643742578257374 1102 70-71 0.38351539987885275 1102 72-73 0.49029441907696736 1102 74-75 0.41972771230732775 1102 76-77 0.259272873379885 1102 78-79 0.36183770694376705 1102 80-81 0.5161006326295094 1102 82-83 0.49163557908072875 1102 84-85 0.4648619103199252 1102 86-87 0.4773102956584516 1102 88-89 0.3860628297877646 1102 90-91 0.4435261391539562 1102 92-93 0.43436581995694823 1102 94-95 0.40923084436369095 1102 96-97 0.6867257157066646 1102 98-99 0.9902670159311739 1102 100-101 1.0832540258103265 1103 1 0.21484905002719756 1103 2 0.24374984857572812 1103 3 0.21240935064934874 1103 4 0.19652689532347267 1103 5 0.19768749417475107 1103 6 0.03631723981460766 1103 7 0.2198545141550099 1103 8 0.039700088617451 1103 9 0.2034126545819248 1103 10-11 0.15428880927732536 1103 12-13 0.22824228087312548 1103 14-15 0.24008306958650394 1103 16-17 0.26253006479480234 1103 18-19 0.25545595204407334 1103 20-21 0.3868492891157089 1103 22-23 0.3532558919574811 1103 24-25 0.3055345382947934 1103 26-27 0.14176832342021584 1103 28-29 0.2159793479407881 1103 30-31 0.20811381892850278 1103 32-33 0.18078271838247417 1103 34-35 0.20335237152691832 1103 36-37 0.24211015851276585 1103 38-39 0.301466888426269 1103 40-41 0.23386825563828495 1103 42-43 0.23198819202188758 1103 44-45 0.207712590506695 1103 46-47 0.287663575031857 1103 48-49 0.25200611839866127 1103 50-51 0.1729472844273232 1103 52-53 0.1364859620545431 1103 54-55 0.31835503011600963 1103 56-57 0.19158908868904945 1103 58-59 0.20910833909608328 1103 60-61 0.23689999490134284 1103 62-63 0.2731035364923571 1103 64-65 0.17670139203258373 1103 66-67 0.11017689937148134 1103 68-69 0.31774404438792203 1103 70-71 0.21282366304411227 1103 72-73 0.13372997623317673 1103 74-75 0.1461121532244931 1103 76-77 0.15607272609895517 1103 78-79 0.27196989240157876 1103 80-81 0.2757905135027414 1103 82-83 0.47707603797014997 1103 84-85 0.26824117733315234 1103 86-87 0.22935104681666374 1103 88-89 0.29737880706705155 1103 90-91 0.3484996750101814 1103 92-93 0.2970570405890456 1103 94-95 0.43365542660280454 1103 96-97 0.3968764274327228 1103 98-99 0.9377069400710809 1103 100-101 0.7977952748303885 1104 1 0.24848990543591754 1104 2 0.23265886979242723 1104 3 0.16049252618701004 1104 4 0.19956203655956983 1104 5 0.1763631048920118 1104 6 0.07578815525455695 1104 7 0.14794636578653098 1104 8 0.10368489832256955 1104 9 0.16229419810588297 1104 10-11 0.1327877002449256 1104 12-13 0.1768966895861297 1104 14-15 0.2708063119426072 1104 16-17 0.2443331512256961 1104 18-19 0.1134013411120236 1104 20-21 0.14380573518277373 1104 22-23 0.22708626733751913 1104 24-25 0.15526899845315967 1104 26-27 0.2833576056939151 1104 28-29 0.20849190586630328 1104 30-31 0.13641946234773883 1104 32-33 0.18855229358275238 1104 34-35 0.1241104354046243 1104 36-37 0.09487512135487464 1104 38-39 0.17440578861423006 1104 40-41 0.279024289991078 1104 42-43 0.11479647639635715 1104 44-45 0.132894305340578 1104 46-47 0.20198971306702873 1104 48-49 0.1961171833142359 1104 50-51 0.13610844730912675 1104 52-53 0.0931716512728471 1104 54-55 0.19679761815319097 1104 56-57 0.11851393511840769 1104 58-59 0.16149072505391615 1104 60-61 0.19611771523367594 1104 62-63 0.13267682096287814 1104 64-65 0.21517963907744075 1104 66-67 0.17985312186832658 1104 68-69 0.12670370113358587 1104 70-71 0.1367149871830904 1104 72-73 0.22625870906362167 1104 74-75 0.1807940271152404 1104 76-77 0.02430304088312596 1104 78-79 0.17868114641002109 1104 80-81 0.2345099805501718 1104 82-83 0.33058580679779936 1104 84-85 0.2357041336721153 1104 86-87 0.30781250557626194 1104 88-89 0.29752933263868186 1104 90-91 0.31229095339666557 1104 92-93 0.2952074405741456 1104 94-95 0.3249800021552538 1104 96-97 0.27914573021355693 1104 98-99 0.6462618203665969 1104 100-101 0.7183938634589673 1105 1 0.22858151277413086 1105 2 0.1748581086075376 1105 3 0.1343395780192438 1105 4 0.13303161142254538 1105 5 0.2181107298387701 1105 6 0.08118128476019848 1105 7 0.19673341345901463 1105 8 0.08277352453156084 1105 9 0.19868632713267687 1105 10-11 0.09194652889984667 1105 12-13 0.16913975806862425 1105 14-15 0.17907676394455052 1105 16-17 0.13194346478663732 1105 18-19 0.19782814872016985 1105 20-21 0.16242733202621906 1105 22-23 0.21279180564992117 1105 24-25 0.23739509857158225 1105 26-27 0.18966320240612333 1105 28-29 0.23856366568865184 1105 30-31 0.19118654188898887 1105 32-33 0.2634348860096125 1105 34-35 0.24872382845160246 1105 36-37 0.21401726313698077 1105 38-39 0.22085975722850293 1105 40-41 0.23641991830899656 1105 42-43 0.26025810354469314 1105 44-45 0.29024565683256043 1105 46-47 0.27637855212709184 1105 48-49 0.23144558540122517 1105 50-51 0.2254285560233953 1105 52-53 0.24509792626547977 1105 54-55 0.26882052781075316 1105 56-57 0.2784372567327509 1105 58-59 0.2258116946612958 1105 60-61 0.13211604392667908 1105 62-63 0.1771692992683711 1105 64-65 0.20261917691894382 1105 66-67 0.29639660403481116 1105 68-69 0.2280391360578804 1105 70-71 0.24168251028393684 1105 72-73 0.3494271702543017 1105 74-75 0.3790235150585417 1105 76-77 0.2260695258028811 1105 78-79 0.31547028718195946 1105 80-81 0.4462408244949785 1105 82-83 0.4762881497784832 1105 84-85 0.3369738950083345 1105 86-87 0.30693132320741157 1105 88-89 0.40645470117320315 1105 90-91 0.6332602536410654 1105 92-93 0.7191938848228894 1105 94-95 0.6545637620507385 1105 96-97 0.6997741538219664 1105 98-99 1.202757429649111 1105 100-101 1.3031264733096535 1106 1 0.16282424734212242 1106 2 0.12051817387714792 1106 3 0.07227391801756866 1106 4 0.12562103807242408 1106 5 0.08407868734284563 1106 6 -0.023304129162987408 1106 7 0.06748116155052486 1106 8 0.0341879240411771 1106 9 0.09818678509529377 1106 10-11 -0.022942254369290538 1106 12-13 0.021875887770690383 1106 14-15 7.136493102279928E-4 1106 16-17 0.02979079116518335 1106 18-19 0.07491798256437221 1106 20-21 0.12919718752770137 1106 22-23 0.207229988971406 1106 24-25 0.12539002719748282 1106 26-27 0.07363748466516284 1106 28-29 0.10741399361214121 1106 30-31 0.20274888746120467 1106 32-33 0.15143996377943125 1106 34-35 0.17029992103246627 1106 36-37 0.10054834374518862 1106 38-39 0.16296479012709142 1106 40-41 0.21231665005159073 1106 42-43 0.2387563856248036 1106 44-45 0.08339543998505405 1106 46-47 0.08687057539682286 1106 48-49 0.13645379450939998 1106 50-51 0.10031986939686988 1106 52-53 0.1204031187463741 1106 54-55 0.15391751225303807 1106 56-57 0.034030198355928576 1106 58-59 0.07785797164998343 1106 60-61 0.1714391978357952 1106 62-63 0.24529028541068953 1106 64-65 0.1537670067022603 1106 66-67 0.08648133669434799 1106 68-69 0.12249606119410572 1106 70-71 0.19287988884723717 1106 72-73 0.2777432022557491 1106 74-75 0.2364388833936104 1106 76-77 0.12798118006617898 1106 78-79 0.30623409648566025 1106 80-81 0.33686436784215346 1106 82-83 0.48697763735756183 1106 84-85 0.46896812762067697 1106 86-87 0.5651876946836758 1106 88-89 0.5661091359669719 1106 90-91 0.5567122847128552 1106 92-93 0.6160754435998648 1106 94-95 0.5929452063912599 1106 96-97 0.6182153217415447 1106 98-99 1.0518026675678058 1106 100-101 1.039734635017215 1107 1 0.17289502246003607 1107 2 0.0795389801421642 1107 3 -0.009108853184709886 1107 4 0.001148500918382922 1107 5 0.008512788971394514 1107 6 0.026825353795096873 1107 7 0.03289052347986399 1107 8 0.03637358747447905 1107 9 0.07092849551622038 1107 10-11 -0.032530600903065476 1107 12-13 0.03207674209124178 1107 14-15 -0.007687783456518105 1107 16-17 0.0543668345931394 1107 18-19 -0.045446861255157955 1107 20-21 0.05724711633432378 1107 22-23 0.1417849280120791 1107 24-25 0.12726331633159305 1107 26-27 0.06273441445069494 1107 28-29 0.03999170563492527 1107 30-31 0.07585768874268695 1107 32-33 0.08227273943129632 1107 34-35 0.10021440443550489 1107 36-37 0.17661545240408572 1107 38-39 0.2944913141103598 1107 40-41 0.1871616309183679 1107 42-43 0.1660963829550539 1107 44-45 0.18768084979193844 1107 46-47 0.09668089843678729 1107 48-49 0.11149519790888007 1107 50-51 0.13834329646815746 1107 52-53 0.09447858817771504 1107 54-55 0.07165626814873605 1107 56-57 0.17364921588729487 1107 58-59 0.11955472345206175 1107 60-61 0.18492364653206295 1107 62-63 0.13234012522558203 1107 64-65 0.16825857918601628 1107 66-67 0.17204336837539813 1107 68-69 0.19555143184462764 1107 70-71 0.20390371193169443 1107 72-73 0.1668221112172219 1107 74-75 0.2232047906642336 1107 76-77 0.09695271161374563 1107 78-79 0.17300315406330924 1107 80-81 0.2138540492517791 1107 82-83 0.31845131805525284 1107 84-85 0.39892870873663355 1107 86-87 0.4290355544327191 1107 88-89 0.35903739973844395 1107 90-91 0.44553546705689584 1107 92-93 0.45966642428865967 1107 94-95 0.4999456513498792 1107 96-97 0.4992798540900374 1107 98-99 0.9617863642840092 1107 100-101 1.077398361990607 1108 1 0.030124723520785324 1108 2 -0.01659789821573554 1108 3 -0.05787281516294129 1108 4 0.002274386026520858 1108 5 0.024009138330811197 1108 6 0.03701408502529091 1108 7 -0.06539849704638101 1108 8 0.021174579346634914 1108 9 -0.017242767743177012 1108 10-11 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-1.2565946432453252 2316 80-81 -1.2563278454031668 2316 82-83 -1.1556691735387759 2316 84-85 -1.2767984006564106 2316 86-87 -1.2598876747225525 2316 88-89 -1.2300534138715626 2316 90-91 -1.260640534304592 2316 92-93 -1.3301816775311117 2316 94-95 -1.4187180978447778 2316 96-97 -1.543573817670051 2316 98-99 -1.0862475416032034 2316 100-101 -1.4389484184574677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 122.0 3 94.0 4 243.0 5 601.0 6 1208.0 7 2164.0 8 3983.0 9 6416.0 10 8700.0 11 11201.0 12 13996.0 13 16721.0 14 19749.0 15 22976.0 16 26417.0 17 30927.0 18 36160.0 19 41139.0 20 44348.0 21 47348.0 22 53231.0 23 63353.0 24 78462.0 25 99304.0 26 126490.0 27 160923.0 28 202590.0 29 250420.0 30 308108.0 31 374299.0 32 455041.0 33 564133.0 34 726787.0 35 1005166.0 36 1294251.0 37 1819093.0 38 1726613.0 39 397904.0 40 649.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.754935674760414 30.75511023443364 29.66312476777329 22.826829323032648 2 21.981470582084246 29.953014192343048 28.023409249571518 20.04210597600119 3 21.26669475059579 28.829597274464636 28.365216559957695 21.53849141498188 4 21.445459623598616 28.693751145898283 28.574841635736746 21.28594759476635 5 21.949308618538705 28.696720027430782 28.80968379208493 20.54428756194558 6 21.893976279411103 28.864225904983197 27.719754745367613 21.522043070238084 7 22.13193298483963 28.902912070237846 28.26017720795394 20.704977736968583 8 21.395522306308028 29.08238251307347 28.147775244912776 21.374319935705728 9 20.990051207416077 28.793916416757597 28.80942234780112 21.406610028025202 10-11 21.115889265743554 29.171613319827777 28.159209648231837 21.55328776619683 12-13 21.103011254485214 29.286688118008268 28.03343282214607 21.576867805360443 14-15 21.2954060866439 29.29037288885038 27.864585667436486 21.54963535706923 16-17 21.427336176416915 29.094230817588613 27.89206905162689 21.586363954367588 18-19 21.38139284152526 29.13188582905709 27.884610692469092 21.602110636948556 20-21 21.515487565665484 29.035064530050526 27.89116222109831 21.558285683185677 22-23 21.564982205711054 29.014926229301263 27.842421738217034 21.577669826770652 24-25 21.430328452505794 28.96058589848158 27.888506801389855 21.720578847622775 26-27 21.658741081849797 28.88293073022272 27.933663104054045 21.524665083873433 28-29 21.651666969016006 28.942072273327256 27.844840460386997 21.561420297269738 30-31 21.611700361753456 28.944877700620992 27.83229423059332 21.611127707032235 32-33 21.640742207088977 28.95062666801377 27.75286599477967 21.655765130117587 34-35 21.65291954159895 28.948230653730295 27.78422230457141 21.61462750009934 36-37 21.656555908929985 28.96984300316145 27.73342256494064 21.640178522967926 38-39 21.684020457262886 28.9599148550187 27.737872845407523 21.61819184231089 40-41 21.671736191627843 29.015428273110196 27.69484374436687 21.61799179089509 42-43 21.70739622257808 28.994637948889345 27.67128196208556 21.62668386644702 44-45 21.674615364774187 29.03802378723721 27.684872835270692 21.602488012717917 46-47 21.70803613686017 29.030532953750495 27.639257064591234 21.622173844798105 48-49 21.71309925861033 29.039196276588275 27.614920179525477 21.632784285275918 50-51 21.754471687388783 29.022098466410394 27.61873564634486 21.604694199855963 52-53 21.708569090754708 29.080455791732827 27.57488190237097 21.636093215141496 54-55 21.73328771918817 29.08120363578599 27.520014700104422 21.66549394492142 56-57 21.755238362374666 29.101546229201205 27.498157721543105 21.64505768688103 58-59 21.741395453337024 29.15913290543246 27.481159227316564 21.61831241391395 60-61 21.730086724576 29.136491793068426 27.470073931448713 21.663347550906856 62-63 21.726050133453583 29.159331557935765 27.493185905158473 21.621432403452175 64-65 21.728830094509178 29.218656806271177 27.47784712396777 21.57466597525188 66-67 21.7153655769456 29.249302674654004 27.45101095244962 21.584320795950774 68-69 21.715998880794952 29.271549191217993 27.44184612862662 21.570605799360436 70-71 21.723416587575304 29.298062070703196 27.39588572202531 21.582635619696187 72-73 21.69150181676704 29.284366531250498 27.40382951496127 21.620302137021188 74-75 21.73557570117732 29.305852778058846 27.407860802668072 21.550710718095765 76-77 21.70164000776005 29.39109597963266 27.393784311233787 21.513479701373505 78-79 21.696112665990714 29.361908681964582 27.375721643011175 21.566257009033528 80-81 21.694409259200427 29.360294918018077 27.382617527871645 21.56267829490985 82-83 21.751910283655292 29.42661687869577 27.30642373477802 21.51504910287091 84-85 21.687765969577526 29.439878812830216 27.352450527704157 21.5199046898881 86-87 21.655924786706464 29.489892617384832 27.30431637290966 21.549866222999047 88-89 21.664896644939084 29.521099243246002 27.312432802579107 21.50157130923581 90-91 21.641934483158696 29.51384964100531 27.309190552755712 21.53502532308028 92-93 21.652953805637623 29.557805047254398 27.29366921145339 21.495571935654585 94-95 21.640388171684595 29.594943924681715 27.29486147459867 21.469806429035017 96-97 21.60760763751435 29.606746188836517 27.271194500670987 21.514451672978147 98-99 21.669623672695103 29.613811725362833 27.189033408202544 21.52753119373952 100-101 21.719675815433373 29.584943887340675 27.169573473280444 21.525806823945505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148513.0 1 84152.5 2 14354.5 3 8027.5 4 6616.0 5 5538.5 6 4943.5 7 5098.0 8 4904.0 9 4415.5 10 4439.5 11 5169.5 12 8844.5 13 8673.5 14 5536.5 15 5955.5 16 6682.0 17 8018.5 18 9896.0 19 11811.0 20 14640.5 21 18912.0 22 25172.5 23 34308.0 24 47106.0 25 63251.5 26 79522.5 27 94204.5 28 108754.0 29 123951.5 30 140228.0 31 159031.0 32 178324.5 33 197772.0 34 217340.0 35 235281.5 36 255369.0 37 277340.5 38 294335.0 39 307800.5 40 324281.0 41 336948.0 42 344652.5 43 358621.0 44 372530.0 45 380488.0 46 382769.5 47 378534.5 48 377800.5 49 379843.0 50 372064.5 51 354874.0 52 337249.5 53 318818.0 54 292769.0 55 265764.0 56 240143.5 57 213871.5 58 192923.5 59 167786.0 60 135654.0 61 105037.5 62 78455.5 63 57831.0 64 42295.5 65 28123.5 66 17099.5 67 11157.0 68 6566.5 69 3119.0 70 1488.0 71 717.5 72 380.0 73 220.0 74 131.5 75 83.5 76 59.0 77 50.5 78 40.5 79 26.5 80 16.0 81 13.5 82 11.5 83 12.0 84 9.5 85 5.5 86 4.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.160387120032904 2 0.0 3 0.0 4 8.066660492185797E-4 5 5.676538864871487E-4 6 1.0954724125190586E-4 7 1.9917680227619252E-4 8 0.0 9 1.0954724125190586E-4 10-11 2.888063633004791E-4 12-13 0.0 14-15 0.0 16-17 2.4897100284524065E-5 18-19 9.958840113809625E-6 20-21 4.9794200569048125E-6 22-23 4.6308606529214753E-4 24-25 0.0 26-27 9.958840113809626E-5 28-29 0.005143740918782671 30-31 0.003739544462735514 32-33 8.465014096738181E-5 34-35 7.867483689909604E-4 36-37 4.481478051214331E-4 38-39 0.001035719371836201 40-41 0.0028283105923219334 42-43 0.0011602048732588213 44-45 6.274069271700063E-4 46-47 0.008305672654917226 48-49 0.008574561337990087 50-51 0.007658348047519602 52-53 0.014315832663601334 54-55 0.00582592146657863 56-57 9.510692308688191E-4 58-59 0.0014091758761040618 60-61 9.958840113809625E-6 62-63 1.0954724125190586E-4 64-65 8.26583729446199E-4 66-67 0.0010556370520638202 68-69 0.0027337016112407423 70-71 0.001991768022761925 72-73 0.005522176843107437 74-75 0.002171027144810498 76-77 0.0015734967379819208 78-79 0.0018025500605995419 80-81 3.0872404352809836E-4 82-83 0.0021560888846397838 84-85 2.9876520341428873E-5 86-87 0.0017876118004288275 88-89 2.1909448250381173E-4 90-91 9.460898108119144E-4 92-93 0.0014739083368438244 94-95 0.0010257605317223913 96-97 0.0010456782119500107 98-99 0.5324842426252299 100-101 1.2946492147952512E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.004133E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.71216219790824 #Duplication Level Percentage of deduplicated Percentage of total 1 90.37316375076354 65.71228140983641 2 5.986774714053273 8.706226681011547 3 1.4130136724182565 3.082298380102147 4 0.5754498399958439 1.673688084101526 5 0.30162660786261003 1.096596141705548 6 0.1902921838619671 0.8301933682779319 7 0.12234854468770495 0.6227359058207299 8 0.09813763977802499 0.5708639985007715 9 0.0825369515418728 0.5401296187050192 >10 0.7922520003678759 12.494864864368955 >50 0.05925678175029358 2.811201375207047 >100 0.004855957040814977 0.48213480175197615 >500 4.8559312982273054E-5 0.024715968492502207 >1k 4.855931298222382E-5 0.1183188720261299 >5k 1.4567793894667148E-4 0.7395370858213342 >10k+ 4.855931298222382E-5 0.4942134442702895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13997 0.13939388507299333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 4.9794200569048125E-6 0.0 0.0 0.0 9.958840113809625E-6 24-25 9.958840113809625E-6 0.0 0.0 0.0 9.958840113809625E-6 26-27 9.958840113809625E-6 0.0 0.0 0.0 9.958840113809625E-6 28-29 1.4938260170714438E-5 0.0 0.0 0.0 9.958840113809625E-6 30-31 1.991768022761925E-5 0.0 0.0 0.0 9.958840113809625E-6 32-33 3.4855940398333685E-5 0.0 0.0 4.9794200569048125E-6 9.958840113809625E-6 34-35 3.98353604552385E-5 0.0 0.0 9.958840113809625E-6 9.958840113809625E-6 36-37 3.98353604552385E-5 0.0 0.0 9.958840113809625E-6 9.958840113809625E-6 38-39 3.98353604552385E-5 0.0 0.0 9.958840113809625E-6 9.958840113809625E-6 40-41 3.98353604552385E-5 0.0 0.0 9.958840113809625E-6 9.958840113809625E-6 42-43 3.98353604552385E-5 0.0 0.0 9.958840113809625E-6 9.958840113809625E-6 44-45 5.477362062595293E-5 0.0 0.0 1.991768022761925E-5 9.958840113809625E-6 46-47 5.9753040682857747E-5 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 48-49 6.971188079666737E-5 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 50-51 8.465014096738181E-5 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 52-53 9.460898108119144E-5 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 54-55 1.6432086187785882E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 56-57 1.6930028193476362E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 58-59 1.8423854210547805E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 60-61 2.1411506244690693E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 62-63 2.788475231866695E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 64-65 3.0872404352809836E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 66-67 3.435799839264321E-4 0.0 0.0 2.9876520341428873E-5 9.958840113809625E-6 68-69 4.132918647230994E-4 0.0 0.0 3.4855940398333685E-5 2.489710028452406E-5 70-71 4.6308606529214753E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 72-73 5.278185260319101E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 74-75 5.57695046373339E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 76-77 6.523040274545304E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 78-79 7.817689489340555E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 80-81 9.01275030299771E-4 0.0 0.0 3.98353604552385E-5 2.9876520341428873E-5 82-83 0.0010307399517792961 9.958840113809625E-6 0.0 4.4814780512143315E-5 2.9876520341428873E-5 84-85 0.0012099990738278694 9.958840113809625E-6 0.0 5.9753040682857747E-5 2.9876520341428873E-5 86-87 0.0014838671769576341 9.958840113809625E-6 0.0 6.473246073976256E-5 2.9876520341428873E-5 88-89 0.0018473648411116853 9.958840113809625E-6 0.0 6.971188079666737E-5 2.9876520341428873E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 415 0.0 36.608177 3 ACTCGCG 325 0.0 23.37722 50-51 CTCGCGT 320 0.0 23.00019 52-53 CGCGTAG 325 0.0 22.64358 54-55 TCGCGTA 315 0.0 22.611557 52-53 CGTAGCG 350 0.0 20.3467 56-57 CGTACGC 365 0.0 20.160936 20-21 ACGTACG 390 0.0 18.868568 20-21 ATCAACG 7430 0.0 18.242384 10-11 CAACGCA 7655 0.0 17.582115 12-13 CGCTAGT 425 0.0 16.7569 90-91 GTATCAA 9835 0.0 16.557133 9 GGTATCA 10095 0.0 16.08367 8 AGTGGTA 9980 0.0 16.031393 5 TCAACGC 7480 0.0 15.613411 10-11 AACGCAG 7800 0.0 14.942392 12-13 ACGCAGA 9015 0.0 14.929683 14-15 AAGCAGT 12105 0.0 14.181071 1 TGGTATC 11500 0.0 14.159949 7 GTGGTAT 12080 0.0 13.362248 6 >>END_MODULE