FastQCFastQC Report
Sat 18 Jun 2016
SRR578575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9421315
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110395711.717653002792074No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT264420.280661457556615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC185440.1968302726317929No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG107950.11458060790876858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGACTTCAACGTCCT103970.11035614455094644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2444500.091.3596045
GTATCAA2458850.091.083749
GGTATCA2461700.090.968168
TGGTATC2463150.090.795137
AAGCAGT2469300.090.791211
GTGGTAT2469600.090.58976
GCAGTGG2473300.090.4479753
AGCAGTG2486750.090.109222
CAGTGGT2483150.089.9591144
TCAACGC2446950.046.24396512-13
ATCAACG2443500.046.20071410-11
CAACGCA2447600.046.1356812-13
AACGCAG2455650.046.08180214-15
ACGCAGA2455150.046.0003214-15
CGCAGAG2460250.045.97509816-17
TATCAAC2465950.045.82245310-11
CAGAGTA2457650.045.58026518-19
AGAGTAC2413400.045.54377718-19
GAGTACT1397650.045.35120420-21
GCAGAGT2483650.045.32503516-17
Produced by FastQC (version 0.11.5)