##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9421315 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.043880286350685 31.0 31.0 34.0 28.0 34.0 2 31.40161368131731 33.0 31.0 34.0 28.0 34.0 3 31.517015299881173 34.0 31.0 34.0 28.0 34.0 4 34.91012698333513 37.0 35.0 37.0 32.0 37.0 5 34.948460804038504 37.0 35.0 37.0 32.0 37.0 6 34.98088218045994 37.0 35.0 37.0 32.0 37.0 7 34.917156044564905 37.0 35.0 37.0 32.0 37.0 8 34.95414185811641 37.0 35.0 37.0 32.0 37.0 9 36.44728257148816 39.0 37.0 39.0 32.0 39.0 10-11 36.34260047562363 39.0 37.0 39.0 32.0 39.0 12-13 36.39529577346687 39.0 37.0 39.0 32.0 39.0 14-15 37.65162750635129 40.0 38.0 41.0 32.0 41.0 16-17 37.62310579786367 40.0 38.0 41.0 32.0 41.0 18-19 37.55249861616983 40.0 38.0 41.0 32.0 41.0 20-21 37.48617443530972 40.0 37.5 41.0 31.5 41.0 22-23 37.21818737617838 39.5 37.0 41.0 31.5 41.0 24-25 37.175496998030525 40.0 37.0 41.0 31.0 41.0 26-27 37.06197362045532 40.0 37.0 41.0 30.0 41.0 28-29 36.88935212335008 40.0 36.5 41.0 30.0 41.0 30-31 36.65362154858425 40.0 36.0 41.0 30.0 41.0 32-33 36.38410519126046 39.0 36.0 41.0 29.0 41.0 34-35 36.21113692727607 39.0 36.0 41.0 28.5 41.0 36-37 35.938886662849086 39.0 35.0 40.5 27.0 41.0 38-39 35.09595815446146 38.0 35.0 40.0 26.5 41.0 40-41 34.872278816704465 38.0 34.0 40.0 26.0 41.0 42-43 34.573001751878586 37.5 34.0 40.0 24.5 41.0 44-45 34.29716881348304 37.0 33.0 40.0 24.0 41.0 46-47 34.168252043371865 37.0 33.0 40.0 24.0 41.0 48-49 34.208481193973455 38.0 33.0 40.0 23.5 41.0 50-51 33.35772447901381 37.0 32.5 39.5 21.5 40.5 52-53 32.98057627836454 37.5 33.0 39.5 13.5 40.5 54-55 32.65106415611834 38.0 32.5 40.0 4.0 41.0 56-57 32.17358187259422 38.0 31.5 40.0 2.0 41.0 58-59 31.901094114781216 37.5 31.0 40.0 2.0 41.0 60-61 31.646651449399577 37.0 31.0 40.0 2.0 41.0 62-63 31.321933509281877 36.5 31.0 40.0 2.0 41.0 64-65 31.028339356024077 36.0 31.0 40.0 2.0 41.0 66-67 30.696992935699527 35.5 30.0 39.0 2.0 41.0 68-69 30.361885310065528 35.0 30.0 39.0 2.0 41.0 70-71 30.05816857837786 35.0 29.5 38.5 2.0 40.5 72-73 29.729834104899368 35.0 29.0 37.5 2.0 40.0 74-75 29.36156221291826 35.0 29.0 37.0 2.0 39.5 76-77 28.985909132642313 35.0 29.0 36.5 2.0 39.0 78-79 28.63402306365937 35.0 28.0 36.0 2.0 39.0 80-81 28.299998938577048 35.0 28.0 36.0 2.0 37.5 82-83 28.020078938025108 34.5 27.0 35.0 2.0 37.0 84-85 27.738314608947903 34.0 27.0 35.0 2.0 37.0 86-87 27.486814154924232 34.0 26.0 35.0 2.0 36.0 88-89 27.219684778611054 34.0 26.0 35.0 2.0 36.0 90-91 26.98548090155143 34.0 25.5 35.0 2.0 36.0 92-93 26.701656509733514 34.0 25.0 35.0 2.0 35.0 94-95 26.40524496845716 34.0 24.5 35.0 2.0 35.0 96-97 26.122174346150192 34.0 23.5 35.0 2.0 35.0 98-99 25.80113535106299 34.0 20.0 35.0 2.0 35.0 100-101 24.936817153444082 33.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6130543029109958 1101 2 0.6165180074443803 1101 3 0.546459164180451 1101 4 0.6016544740812648 1101 5 0.5789261164926103 1101 6 0.5725373863794658 1101 7 0.515070360597008 1101 8 0.5536999191161485 1101 9 0.7109345060747856 1101 10-11 0.747764180761564 1101 12-13 0.7685656184189824 1101 14-15 0.906761706575594 1101 16-17 0.8669862201048772 1101 18-19 0.8841782806946838 1101 20-21 0.9220581104329781 1101 22-23 0.9399966070646784 1101 24-25 0.8891803325459691 1101 26-27 0.9419984271659629 1101 28-29 1.020567108695026 1101 30-31 1.1056159890561972 1101 32-33 1.1527152450035842 1101 34-35 1.2115988379783929 1101 36-37 1.1386950265538758 1101 38-39 1.2117284037595581 1101 40-41 1.1926403082717414 1101 42-43 1.2255269180951274 1101 44-45 1.4082473830812177 1101 46-47 1.3420397617516855 1101 48-49 1.2505580857314058 1101 50-51 1.3332147130390624 1101 52-53 1.1624036152770643 1101 54-55 1.1322982387271168 1101 56-57 1.1443708296744788 1101 58-59 1.1471600712212826 1101 60-61 1.0699420521063416 1101 62-63 1.0414756989678864 1101 64-65 1.0756396265341799 1101 66-67 1.1602410736983515 1101 68-69 1.0230731140153075 1101 70-71 1.088015663412147 1101 72-73 1.0663322825599018 1101 74-75 1.045529971936059 1101 76-77 1.0059484781388726 1101 78-79 1.064478220488489 1101 80-81 0.983675055478777 1101 82-83 1.0268057102098531 1101 84-85 1.0060152314188535 1101 86-87 0.9870722236579113 1101 88-89 0.9963624904072432 1101 90-91 0.9951136777866125 1101 92-93 1.0976862592455916 1101 94-95 1.1502746879978254 1101 96-97 1.1948881129366598 1101 98-99 1.193004208837305 1101 100-101 1.3220363249138742 1102 1 0.5023743136150891 1102 2 0.4520925914596816 1102 3 0.4969334384445183 1102 4 0.4673437103110274 1102 5 0.5076211961808994 1102 6 0.5749892433980861 1102 7 0.5738108358427851 1102 8 0.49235738542928686 1102 9 0.6525188851361889 1102 10-11 0.6442052994852574 1102 12-13 0.6050790664545573 1102 14-15 0.7778624225721131 1102 16-17 0.7774683625241892 1102 18-19 0.8337073685431804 1102 20-21 0.8522752564139111 1102 22-23 0.8443456215892766 1102 24-25 0.898024481641329 1102 26-27 0.9642345235820855 1102 28-29 1.036425352333957 1102 30-31 1.0320173256333476 1102 32-33 1.1117592442841655 1102 34-35 1.0754421099983702 1102 36-37 1.1309306900100324 1102 38-39 1.144950331620656 1102 40-41 1.1444995068756398 1102 42-43 1.1542729799437268 1102 44-45 1.252742466780795 1102 46-47 1.2935897076173788 1102 48-49 1.2041565738209457 1102 50-51 1.1732544047738998 1102 52-53 1.029473367691601 1102 54-55 0.9602792040127923 1102 56-57 0.8403783404231078 1102 58-59 0.9087370399097559 1102 60-61 0.9746382508206324 1102 62-63 1.0026566225940883 1102 64-65 0.9820849808830836 1102 66-67 0.9775645670522799 1102 68-69 0.8969302381259503 1102 70-71 0.9074758431092782 1102 72-73 0.9245708343782582 1102 74-75 0.8338718607794853 1102 76-77 0.927030038883796 1102 78-79 0.9734427923784565 1102 80-81 0.963498272720706 1102 82-83 0.9937867846787682 1102 84-85 0.989094626791573 1102 86-87 0.9700689212240867 1102 88-89 1.0013082067592798 1102 90-91 0.9694325738801588 1102 92-93 0.979199202482441 1102 94-95 1.0777913973147193 1102 96-97 1.1335428604738063 1102 98-99 1.1849178491928498 1102 100-101 1.2396444361504635 1103 1 0.46285217416474467 1103 2 0.4705187446118657 1103 3 0.39071837055264425 1103 4 0.46352522009200925 1103 5 0.48908374172503244 1103 6 0.5429937829831104 1103 7 0.43501648853482777 1103 8 0.45546821457912756 1103 9 0.5637022669668283 1103 10-11 0.5790035858990024 1103 12-13 0.6381714999468002 1103 14-15 0.690178441571561 1103 16-17 0.7795205204214781 1103 18-19 0.7521911552454839 1103 20-21 0.7896099178373746 1103 22-23 0.8607717778700916 1103 24-25 0.7713321971475224 1103 26-27 0.8194376558244798 1103 28-29 0.8829799953806727 1103 30-31 0.8963818031827913 1103 32-33 0.961567324599514 1103 34-35 0.962205813038814 1103 36-37 0.9789751483370921 1103 38-39 0.8898802532565497 1103 40-41 0.9683704855380171 1103 42-43 0.8698636266644044 1103 44-45 0.8054482668536593 1103 46-47 1.0655237141154004 1103 48-49 1.0960641888912335 1103 50-51 1.1046090449532713 1103 52-53 0.9582090708331421 1103 54-55 0.9015706714768754 1103 56-57 0.8544278007306829 1103 58-59 0.8574125200153766 1103 60-61 0.9341914049087094 1103 62-63 0.7451137574818176 1103 64-65 0.6588920482843754 1103 66-67 0.6589889839923693 1103 68-69 0.8764846716862316 1103 70-71 0.7901095051078819 1103 72-73 0.837361867962656 1103 74-75 0.828868144041369 1103 76-77 0.8730014657350544 1103 78-79 0.914529715619576 1103 80-81 0.9490755656915866 1103 82-83 0.9462332067014181 1103 84-85 0.9787635290783996 1103 86-87 0.9702056627029663 1103 88-89 0.9987777996402052 1103 90-91 0.9045267665335146 1103 92-93 0.9632233597932185 1103 94-95 0.9340576764697914 1103 96-97 0.9075990609164997 1103 98-99 1.0241493646887392 1103 100-101 1.084836216966341 1104 1 0.3614511536716911 1104 2 0.37955083372907694 1104 3 0.3973921067799928 1104 4 0.35462968222769575 1104 5 0.45195289641410596 1104 6 0.44223927730609347 1104 7 0.4771237197013747 1104 8 0.3495483925807932 1104 9 0.5177442099074909 1104 10-11 0.5499460947000614 1104 12-13 0.5639965735274259 1104 14-15 0.6428121196132963 1104 16-17 0.6122210755869801 1104 18-19 0.6656354104549251 1104 20-21 0.6777817937609925 1104 22-23 0.6878732345399641 1104 24-25 0.6768170546303551 1104 26-27 0.7605956861721026 1104 28-29 0.8246313826963245 1104 30-31 0.9237612298757085 1104 32-33 0.8936090937898413 1104 34-35 0.8680307170636112 1104 36-37 0.8608317521332012 1104 38-39 0.9351220728786487 1104 40-41 0.8723287857457294 1104 42-43 1.004176673282906 1104 44-45 0.9892030547945225 1104 46-47 1.1415289640653796 1104 48-49 1.071451188138255 1104 50-51 1.0089738574762919 1104 52-53 0.9031163378327847 1104 54-55 0.8532607513657453 1104 56-57 0.7569211158423883 1104 58-59 0.8358114044490073 1104 60-61 0.8593462253026516 1104 62-63 0.7389915714913045 1104 64-65 0.6058287143336649 1104 66-67 0.7633040704961935 1104 68-69 0.7554282109384225 1104 70-71 0.6740543836624688 1104 72-73 0.7024695296434302 1104 74-75 0.795188103810478 1104 76-77 0.847731752403913 1104 78-79 0.712668731668245 1104 80-81 0.8452136859350965 1104 82-83 0.8186929804968592 1104 84-85 0.8064142667333591 1104 86-87 0.7829210675411957 1104 88-89 0.8169981179300052 1104 90-91 0.8269926900566631 1104 92-93 0.6856896544971605 1104 94-95 0.5628975264523888 1104 96-97 0.7302306825389984 1104 98-99 0.9782661374541171 1104 100-101 0.8995195707375494 1105 1 0.208523906848324 1105 2 0.12626282444841763 1105 3 0.1408621521955311 1105 4 0.15850168798109365 1105 5 0.11173933953015336 1105 6 0.05774100358770795 1105 7 0.09325584771592332 1105 8 0.10366902987146887 1105 9 0.06463788154086814 1105 10-11 0.08891450608118134 1105 12-13 0.04813334366313171 1105 14-15 -0.04392893996750047 1105 16-17 -0.018860281522826483 1105 18-19 -0.09043340356475227 1105 20-21 0.03459633811012708 1105 22-23 -0.040602237201191826 1105 24-25 -0.12068984593064158 1105 26-27 -0.3683939127570639 1105 28-29 -0.17123267658187302 1105 30-31 -0.35121136902019146 1105 32-33 -0.28849297528120843 1105 34-35 -0.17704958078538624 1105 36-37 -0.13492570909564705 1105 38-39 -0.14408222400791004 1105 40-41 -0.11899430924285781 1105 42-43 -0.02870347516770977 1105 44-45 0.02354405359721312 1105 46-47 0.01177321583512736 1105 48-49 0.027145003309890114 1105 50-51 0.041649284942813836 1105 52-53 -0.07928814378345095 1105 54-55 -0.11056377238912063 1105 56-57 0.014476494376935989 1105 58-59 -0.008467695518785234 1105 60-61 -0.11228210900047131 1105 62-63 -0.009313793769493373 1105 64-65 -0.05417311752055909 1105 66-67 -0.09661528048803447 1105 68-69 -0.098020201246932 1105 70-71 -0.02574040727586535 1105 72-73 -0.01431644982012159 1105 74-75 0.03521441219846011 1105 76-77 0.009866452772744339 1105 78-79 0.023066856543110248 1105 80-81 0.07520118595583014 1105 82-83 0.10812342937141395 1105 84-85 0.044742614989349505 1105 86-87 0.10263595731942132 1105 88-89 0.20913144182469878 1105 90-91 0.19432287627712697 1105 92-93 0.20448193670087988 1105 94-95 0.2699331972333745 1105 96-97 0.28191010976677333 1105 98-99 0.2530629674809113 1105 100-101 0.25224715965294564 1106 1 -0.14616427180601832 1106 2 -0.1262323501307776 1106 3 -0.16305719880299208 1106 4 -0.14815198495661264 1106 5 -0.18214860435845281 1106 6 -0.17685035351375689 1106 7 -0.23591856352104656 1106 8 -0.23145677694735411 1106 9 -0.17710768770845675 1106 10-11 -0.4120277978033613 1106 12-13 -0.40129510260626944 1106 14-15 -0.4122736217390752 1106 16-17 -0.3935390730113042 1106 18-19 -0.4211487731723622 1106 20-21 -0.24183196937888596 1106 22-23 -0.2688018519960309 1106 24-25 -0.32934446264084727 1106 26-27 -0.3629924892234868 1106 28-29 -0.410257704106364 1106 30-31 -0.40451946319967647 1106 32-33 -0.32658179470703175 1106 34-35 -0.3045421363252885 1106 36-37 -0.40953795053371067 1106 38-39 -0.35845818383153016 1106 40-41 -0.3962140182853844 1106 42-43 -0.45358065407864245 1106 44-45 -0.4552933440558746 1106 46-47 -0.5627291849167833 1106 48-49 -0.7413001554896894 1106 50-51 -0.7452856673745458 1106 52-53 -0.808429179051771 1106 54-55 -0.7813589932730309 1106 56-57 -0.7003784164861884 1106 58-59 -0.680068743260275 1106 60-61 -0.8723008039749445 1106 62-63 -0.7590783422619936 1106 64-65 -0.7316420323316244 1106 66-67 -0.8969540142279833 1106 68-69 -0.9622800726218834 1106 70-71 -0.797242321822285 1106 72-73 -0.8126993538935388 1106 74-75 -0.9112844445977508 1106 76-77 -0.958820297241207 1106 78-79 -0.8330503405714751 1106 80-81 -0.8483512132568478 1106 82-83 -0.9441566475909582 1106 84-85 -1.023264344972187 1106 86-87 -0.9353915748401391 1106 88-89 -1.043140062556656 1106 90-91 -0.9429152230379216 1106 92-93 -0.9401971764091144 1106 94-95 -0.9512795431832046 1106 96-97 -0.9201650597503246 1106 98-99 -0.9933232486652805 1106 100-101 -1.1484947973335373 1107 1 -0.38096204150857815 1107 2 -0.46831074499870695 1107 3 -0.4098535212930372 1107 4 -0.29443462540181997 1107 5 -0.3421907112007503 1107 6 -0.3792652171720263 1107 7 -0.3126904293350634 1107 8 -0.27150469991583037 1107 9 -0.3352052100145855 1107 10-11 -0.3452569077607137 1107 12-13 -0.43584808172648337 1107 14-15 -0.6193101405449539 1107 16-17 -0.524957989077997 1107 18-19 -0.5023284165080923 1107 20-21 -0.3936938696424832 1107 22-23 -0.47176745126577657 1107 24-25 -0.48826142994806787 1107 26-27 -0.3527566255459149 1107 28-29 -0.40329595462230117 1107 30-31 -0.4409853624141675 1107 32-33 -0.40067717555799476 1107 34-35 -0.4571042111097725 1107 36-37 -0.6265734666631815 1107 38-39 -0.5888283776182845 1107 40-41 -0.7537887804170538 1107 42-43 -0.6262335423989995 1107 44-45 -0.599059935880156 1107 46-47 -0.6202315667081209 1107 48-49 -0.6288150738672371 1107 50-51 -0.7956207881299022 1107 52-53 -0.8429534057361465 1107 54-55 -0.6983804723967637 1107 56-57 -0.7440396113183425 1107 58-59 -0.6532451328032316 1107 60-61 -0.6137711334744012 1107 62-63 -0.629351484951826 1107 64-65 -0.6289128993194453 1107 66-67 -0.6720013067836739 1107 68-69 -0.6109809827635218 1107 70-71 -0.6104424244417963 1107 72-73 -0.6824100862745439 1107 74-75 -0.7400382558350032 1107 76-77 -0.7255993631670812 1107 78-79 -0.7015136106074671 1107 80-81 -0.6168534178924574 1107 82-83 -0.6995921231958881 1107 84-85 -0.7541614181869072 1107 86-87 -0.755508218368476 1107 88-89 -0.8578142073384001 1107 90-91 -0.7696383408730654 1107 92-93 -0.73124166068229 1107 94-95 -0.7230630811432412 1107 96-97 -0.7813503823676626 1107 98-99 -0.7899663945336144 1107 100-101 -0.9122863479838408 1108 1 -0.8242145754578445 1108 2 -0.8030938679648685 1108 3 -0.6784746194558373 1108 4 -0.6617937512014507 1108 5 -0.8757989187944233 1108 6 -0.7815185425090334 1108 7 -0.622608924231649 1108 8 -0.7461361219990152 1108 9 -0.841924756757308 1108 10-11 -1.1096745427873742 1108 12-13 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-0.24563475992740536 2305 70-71 -0.2644269103722614 2305 72-73 -0.18769074724805535 2305 74-75 -0.2016589816751697 2305 76-77 -0.224213203985979 2305 78-79 -0.24194332836036736 2305 80-81 -0.18524102283979005 2305 82-83 -0.24124573461822862 2305 84-85 -0.1888804142360634 2305 86-87 -0.07887230583676086 2305 88-89 -0.0706372288740802 2305 90-91 -0.028707420612278156 2305 92-93 -0.04771303128572413 2305 94-95 0.026502841498906804 2305 96-97 -0.01960466291495422 2305 98-99 -0.01680437748972352 2305 100-101 0.0404053863168663 2306 1 -0.35185448663589725 2306 2 -0.3583378102358239 2306 3 -0.3336101144630774 2306 4 -0.5137186388883137 2306 5 -0.3823574247958632 2306 6 -0.37400940563924934 2306 7 -0.39100232512051747 2306 8 -0.39190350157964104 2306 9 -0.5636410918119807 2306 10-11 -0.7585393348023928 2306 12-13 -0.7629234092070263 2306 14-15 -0.8615949624512069 2306 16-17 -0.7023899615606197 2306 18-19 -0.6583254624603398 2306 20-21 -0.6870458422639345 2306 22-23 -0.6391574306283374 2306 24-25 -0.6477429396072409 2306 26-27 -0.7161232691696924 2306 28-29 -0.5915468417705796 2306 30-31 -0.6610360426357431 2306 32-33 -0.7486033340723068 2306 34-35 -0.6841047585433628 2306 36-37 -0.7180057550919585 2306 38-39 -0.7257709536169301 2306 40-41 -0.7837999142923096 2306 42-43 -0.7988084206333284 2306 44-45 -0.8819507349754332 2306 46-47 -0.8293647048492332 2306 48-49 -0.9152890026447054 2306 50-51 -0.8310920608071228 2306 52-53 -0.9064111699631283 2306 54-55 -0.7991939912084156 2306 56-57 -0.9835493036736906 2306 58-59 -0.9733695440008105 2306 60-61 -0.8258982441754199 2306 62-63 -0.9135391013483698 2306 64-65 -1.0197645876426584 2306 66-67 -0.9767819617516835 2306 68-69 -0.9697672044763586 2306 70-71 -1.0641597053501002 2306 72-73 -1.0642164283860254 2306 74-75 -1.1079770546904761 2306 76-77 -1.0380150387007916 2306 78-79 -1.112590315467159 2306 80-81 -1.2879748031950982 2306 82-83 -1.2933973228293851 2306 84-85 -1.2188435786602199 2306 86-87 -1.1855799703996723 2306 88-89 -1.22232063084531 2306 90-91 -1.1969944530491716 2306 92-93 -1.1986513172138444 2306 94-95 -1.2708895155382578 2306 96-97 -1.2086405936066633 2306 98-99 -1.1637053820245278 2306 100-101 -1.2282088556858106 2307 1 -0.706029461648054 2307 2 -0.7317471129402335 2307 3 -0.7343896737651718 2307 4 -0.8413420646913963 2307 5 -0.8331759584962484 2307 6 -0.6720569204118974 2307 7 -0.6686233906246102 2307 8 -0.674647563473691 2307 9 -0.7865791783470542 2307 10-11 -0.8727297934999783 2307 12-13 -0.8719080284318679 2307 14-15 -1.0964231171768972 2307 16-17 -0.9977281803096574 2307 18-19 -1.0078556497539424 2307 20-21 -0.9330855490062433 2307 22-23 -0.9670138080166382 2307 24-25 -1.0501427309580578 2307 26-27 -1.1447779517826717 2307 28-29 -1.0981977905828657 2307 30-31 -1.0563865808428474 2307 32-33 -1.252544482854944 2307 34-35 -1.0949170462909237 2307 36-37 -1.1384035783690294 2307 38-39 -1.1226197311526391 2307 40-41 -1.2456402068478027 2307 42-43 -1.3193576767855646 2307 44-45 -1.34831542759472 2307 46-47 -1.338479870939537 2307 48-49 -1.3813526951113388 2307 50-51 -1.2285515075517992 2307 52-53 -1.1907420521833387 2307 54-55 -1.0647203155517104 2307 56-57 -1.0494693683799987 2307 58-59 -1.1420761013952507 2307 60-61 -1.107572409831441 2307 62-63 -1.0836856814984515 2307 64-65 -1.057911772475908 2307 66-67 -1.0273964722619624 2307 68-69 -1.0148070671658864 2307 70-71 -1.0468721125641416 2307 72-73 -1.0841631724729766 2307 74-75 -1.0749434141957117 2307 76-77 -1.03950940105997 2307 78-79 -1.0818931461076744 2307 80-81 -1.0488672961354446 2307 82-83 -1.134042612755053 2307 84-85 -1.1169539142947364 2307 86-87 -1.1927741756764547 2307 88-89 -1.199906536889035 2307 90-91 -1.1559276931243296 2307 92-93 -1.0790682731799706 2307 94-95 -1.0261162906459305 2307 96-97 -1.1580059616250402 2307 98-99 -1.3025124875355978 2307 100-101 -1.3481543575917598 2308 1 -1.6737852421769794 2308 2 -1.2238145905055227 2308 3 -1.253169208884529 2308 4 -1.2061465706373227 2308 5 -1.508337964042397 2308 6 -1.5939927679884676 2308 7 -1.462163974428961 2308 8 -1.3871692251566046 2308 9 -1.4682591962820766 2308 10-11 -1.530071477345416 2308 12-13 -1.4177915504721312 2308 14-15 -1.898115116195818 2308 16-17 -2.022375784162975 2308 18-19 -1.7418693960883047 2308 20-21 -3.0056990079017822 2308 22-23 -3.1743403193007254 2308 24-25 -2.3489224912767526 2308 26-27 -2.7226289301406936 2308 28-29 -2.4166332006178806 2308 30-31 -2.221892949627403 2308 32-33 -2.5755015060547564 2308 34-35 -2.754532360569904 2308 36-37 -3.0323378692662715 2308 38-39 -3.068461353516348 2308 40-41 -2.713311218193539 2308 42-43 -2.8275608953898654 2308 44-45 -2.831065870420204 2308 46-47 -2.943543061447688 2308 48-49 -2.6692341074118 2308 50-51 -2.6762123465955483 2308 52-53 -2.623004214398307 2308 54-55 -2.301300016676162 2308 56-57 -2.1796194212361826 2308 58-59 -2.065547323981157 2308 60-61 -1.9718530855259893 2308 62-63 -2.0890772956926966 2308 64-65 -2.06508251911038 2308 66-67 -2.245618079572278 2308 68-69 -2.055173704553088 2308 70-71 -2.003998599720493 2308 72-73 -2.0168421242692673 2308 74-75 -1.8037779506596117 2308 76-77 -1.7142620571211147 2308 78-79 -1.798389582749632 2308 80-81 -2.4209058804837866 2308 82-83 -2.2502410897331764 2308 84-85 -1.9866845559227855 2308 86-87 -1.8157803762713556 2308 88-89 -1.7661375067242666 2308 90-91 -2.0049610515880616 2308 92-93 -2.0887065519351182 2308 94-95 -2.2443651627814596 2308 96-97 -2.2126919516725785 2308 98-99 -2.2084947278083114 2308 100-101 -2.3488491592155434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69286.0 3 13474.0 4 12322.0 5 12085.0 6 14150.0 7 17592.0 8 21581.0 9 25020.0 10 28816.0 11 34573.0 12 34703.0 13 38354.0 14 42842.0 15 49775.0 16 62176.0 17 83490.0 18 127672.0 19 205338.0 20 295416.0 21 212493.0 22 116780.0 23 100117.0 24 103301.0 25 108450.0 26 115837.0 27 129117.0 28 149649.0 29 173852.0 30 205058.0 31 244955.0 32 298884.0 33 375881.0 34 494481.0 35 716149.0 36 1166451.0 37 1589977.0 38 1585798.0 39 344988.0 40 432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.447933959950891 46.66307513261968 25.277985478605896 17.611005428823535 2 16.760211588946294 48.391292938500456 19.031998631619988 15.816496840933262 3 42.413057660007546 21.379296498255858 19.932099709045797 16.275546132690796 4 16.550863337132437 19.87787710014558 20.931183659910545 42.64007590281144 5 16.609830664797972 45.875741673439855 21.887754836460296 15.626672825301876 6 42.58910262581562 20.931789168392477 19.80506278030571 16.674045425486195 7 17.421987678008033 20.34851384175526 46.69704636477461 15.5324521154621 8 42.14567407006563 21.104776817980284 20.66357473150255 16.085974380451535 9 41.76836346208045 20.708738106270378 21.324698885026706 16.198199546622465 10-11 16.27251707144744 33.527836586271 33.672856844416934 16.526789497864634 12-13 16.09205420374809 21.108081956912997 33.59662929644291 29.203234542895995 14-15 16.286170644216533 46.531635574965804 20.6603493727406 16.52184440807707 16-17 29.10029867288868 20.89063929542133 20.77487880627223 29.23418322541776 18-19 16.447615831991776 33.58446588792383 20.75081202043932 29.21710625964508 20-21 29.256460913544164 33.61777192909646 20.777987597686817 16.34777955967256 22-23 29.146504631989583 20.80187549976646 33.62078733394432 16.43083253429963 24-25 16.341481963804007 33.415558925366 21.201173667721182 29.041785443108814 26-27 16.607755904640513 25.928290969789796 40.826912112459915 16.637041013109783 28-29 27.06274173951989 20.678852097805063 35.73209098213242 16.526315180542625 30-31 24.93666707356555 21.776570054719055 36.17122658783443 17.115536283880967 32-33 19.642200102013526 24.010350164539403 37.427421769151906 18.92002796429516 34-35 19.17347011885586 23.114076684454798 38.11793240155715 19.59452079513219 36-37 18.953549107908128 23.188794986839902 38.219040867363816 19.63861503788815 38-39 19.227299709878487 23.262416805341015 38.012573435929795 19.4977100488507 40-41 19.34414057534778 23.000377613941623 38.09682573421763 19.558656076492962 42-43 19.601588884980444 23.294452573865872 38.00244406284636 19.101514478307323 44-45 19.15688142835414 23.5782750248932 37.68895185765709 19.575891689095577 46-47 19.51286538028697 23.30495352792048 37.6264018325914 19.555779259201156 48-49 19.491870844230426 23.348086404873154 37.88707972082633 19.272963030070088 50-51 19.723455076045077 23.44371300277548 37.098242019001916 19.734589902177525 52-53 19.97517804082949 24.391626853310513 35.94575729693062 19.68743780892937 54-55 20.629969451140397 25.226201388205745 34.34930440106915 19.79452475958471 56-57 20.83409697884389 25.73923320939471 33.18512632339612 20.241543488365284 58-59 20.829328496054906 26.555018826034015 32.440603319382 20.17504935852908 60-61 21.120385951814676 26.680439730731393 31.96379433145226 20.23537998600167 62-63 21.123013177247028 26.56195490851841 32.14430268723093 20.17072922700363 64-65 20.69553691839654 26.30836968734084 32.26511722952018 20.730976164742447 66-67 20.655117171681425 26.01898970905001 32.862716679230864 20.463176440037703 68-69 20.67173752491971 25.716273741315867 33.05048171240234 20.56150702136209 70-71 20.4669344520288 25.80635699487457 33.37212152566134 20.354587027435294 72-73 20.152219399113534 25.475111126696653 33.36788010749775 21.004789366692066 74-75 20.467385447350566 25.834186285632242 32.655992767665886 21.0424354993513 76-77 20.94144537820842 26.406653591774486 32.299149961956125 20.352751068060968 78-79 20.923393657659812 26.07493863696736 32.11628080761202 20.885386897760803 80-81 20.69547144918122 26.40654927241024 32.076892633896676 20.821086644511865 82-83 21.51919476065865 26.67423062276873 31.330670221449747 20.475904395122875 84-85 21.220658544274524 27.012712928250938 30.873340385886866 20.893288141587675 86-87 20.985688473603677 27.248400011987556 31.01617491119325 20.749736603215513 88-89 20.958693224044975 26.997784984108282 30.928352920729086 21.11516887111766 90-91 20.83379498691242 26.912381684498055 31.091240655400888 21.16258267318864 92-93 20.806986430180025 27.494447511739047 30.948465127907603 20.75010093017333 94-95 20.83929636413698 26.973620565200708 30.588471674584895 21.598611396077423 96-97 20.805386001543514 26.815825530937072 31.066867514484862 21.311920953034555 98-99 21.357416669456796 26.95655847872566 30.578981129688948 21.107043722128598 100-101 21.50897166765719 26.868543005253592 30.43703295591452 21.185452371174694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9743.0 1 5802.0 2 1338.0 3 702.0 4 585.5 5 545.5 6 654.0 7 828.5 8 788.5 9 904.5 10 1529.5 11 9063.5 12 162384.0 13 180612.5 14 47251.5 15 43313.5 16 47027.5 17 51382.0 18 55421.5 19 58231.5 20 60033.5 21 62011.5 22 64708.0 23 69169.0 24 76763.0 25 87050.5 26 96263.5 27 103975.0 28 113935.5 29 125514.0 30 134892.5 31 143757.0 32 155344.0 33 168638.5 34 186504.0 35 200877.5 36 211038.5 37 225201.5 38 238370.0 39 251587.0 40 267221.5 41 277727.0 42 281187.5 43 289522.0 44 303295.5 45 310488.0 46 308734.5 47 309280.5 48 317102.5 49 313916.0 50 299861.5 51 288741.0 52 282929.5 53 282595.5 54 265098.5 55 240673.0 56 236781.0 57 224618.5 58 217509.0 59 186124.0 60 129225.0 61 98452.5 62 73435.5 63 53699.5 64 38889.0 65 26310.5 66 17026.5 67 10702.0 68 6142.0 69 3479.5 70 2244.0 71 1582.0 72 1276.5 73 1026.0 74 809.0 75 722.5 76 654.0 77 548.5 78 476.0 79 426.0 80 351.5 81 294.0 82 263.0 83 209.0 84 161.0 85 130.5 86 112.5 87 97.5 88 76.5 89 57.0 90 45.5 91 38.5 92 26.0 93 15.5 94 8.0 95 9.0 96 7.5 97 2.0 98 3.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05016284881675223 2 0.0926091527562766 3 0.08285467580693354 4 0.05373984417249609 5 0.08463786637003433 6 0.0013586213814101322 7 0.001178179479191599 8 0.004903774048527196 9 0.00965894888346266 10-11 0.009627106194835859 12-13 0.01656350520070712 14-15 0.011564203086299523 16-17 0.0014700707916039321 18-19 6.580822316205328E-4 20-21 8.491383633813326E-5 22-23 4.1926206691953296E-4 24-25 8.438312486101992E-4 26-27 0.001533756168857532 28-29 0.0015549846279420657 30-31 0.004935616737153996 32-33 1.645205579051332E-4 34-35 0.0025474150901439977 36-37 3.3965534535253304E-4 38-39 0.0027862352548449977 40-41 0.003370017879669664 42-43 0.001241864856445199 44-45 7.005391497895995E-4 46-47 0.0012790146598431321 48-49 0.0023988158765522647 50-51 0.004484511981607663 52-53 0.1306346300914469 54-55 0.14086674737019195 56-57 0.12687719283348448 58-59 0.12037067012407503 60-61 0.10818022749478177 62-63 0.11276557465704098 64-65 0.11510070515633965 66-67 0.12354432475721276 68-69 0.13637692827381317 70-71 0.12380968049576944 72-73 0.11553588856757256 74-75 0.12048742664903997 76-77 0.13206224396488175 78-79 0.14624285463335002 80-81 0.13606911561708743 82-83 0.11564733797776638 84-85 0.12189911917816143 86-87 0.12266865081997577 88-89 0.11913941949717209 90-91 0.12953075021905117 92-93 0.1466992665036675 94-95 0.15650681460072186 96-97 0.1576903011946846 98-99 0.13957711848080656 100-101 0.12884613241357495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9421315.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.06795114511146 #Duplication Level Percentage of deduplicated Percentage of total 1 91.38164405126838 46.666733339701395 2 5.440938587433541 5.557151719332157 3 1.2107397112771332 1.8548998927484097 4 0.5142289989393294 1.0504248558093296 5 0.28682427369695285 0.7323763998194033 6 0.1947985837702866 0.5968778735470739 7 0.12200994031978524 0.436155837002813 8 0.10208204381858389 0.41704966612164557 9 0.08133659973040688 0.3738324151207713 >10 0.6290764140646474 6.083831795432362 >50 0.016180922309939106 0.5802604341890065 >100 0.013981614057616724 1.4536640476554887 >500 0.0028124333874262513 1.0031725716666622 >1k 0.002715452925452125 2.8052727597614218 >5k 3.8792184649316067E-4 1.4122815556756272 >10k+ 2.4245115405822543E-4 28.976014836416397 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1103957 11.717653002792074 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 26442 0.280661457556615 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC 18544 0.1968302726317929 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10795 0.11458060790876858 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGACTTCAACGTCCT 10397 0.11035614455094644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.307114771133329E-6 0.0 12-13 0.0 0.0 0.0 1.0614229542266658E-5 0.0 14-15 0.0 0.0 0.0 2.1228459084533315E-5 0.0 16-17 0.0 0.0 0.0 2.1228459084533315E-5 0.0 18-19 5.307114771133329E-6 0.0 0.0 2.1228459084533315E-5 0.0 20-21 1.0614229542266658E-5 0.0 0.0 2.1228459084533315E-5 0.0 22-23 1.0614229542266658E-5 1.0614229542266658E-5 0.0 2.1228459084533315E-5 0.0 24-25 2.1228459084533315E-5 1.0614229542266658E-5 0.0 2.1228459084533315E-5 0.0 26-27 2.1228459084533315E-5 1.0614229542266658E-5 0.0 2.6535573855666646E-5 0.0 28-29 2.1228459084533315E-5 1.0614229542266658E-5 0.0 3.1842688626799976E-5 0.0 30-31 4.245691816906663E-5 1.0614229542266658E-5 0.0 3.1842688626799976E-5 0.0 32-33 6.368537725359995E-5 1.0614229542266658E-5 0.0 5.307114771133329E-5 0.0 34-35 7.42996067958666E-5 1.0614229542266658E-5 0.0 5.307114771133329E-5 0.0 36-37 8.491383633813326E-5 1.0614229542266658E-5 0.0 5.307114771133329E-5 0.0 38-39 8.491383633813326E-5 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 40-41 1.0083518065153325E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 42-43 1.1675652496493324E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 44-45 1.273707545071999E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 46-47 1.5921344313399988E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 48-49 1.5921344313399988E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 50-51 1.8574901698966654E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 52-53 2.2820593515873315E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 54-55 2.547415090143998E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 56-57 2.547415090143998E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 58-59 2.971984271834664E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 60-61 3.290411158102664E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 62-63 3.502695748947997E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 64-65 3.980336078349997E-4 1.0614229542266658E-5 0.0 6.368537725359995E-5 0.0 66-67 4.564118703174663E-4 1.0614229542266658E-5 0.0 7.42996067958666E-5 0.0 68-69 5.094830180287996E-4 1.0614229542266658E-5 0.0 7.42996067958666E-5 0.0 70-71 5.519399361978662E-4 1.0614229542266658E-5 0.0 7.42996067958666E-5 0.0 72-73 6.103181986803329E-4 1.0614229542266658E-5 0.0 7.42996067958666E-5 0.0 74-75 6.421608873071328E-4 1.0614229542266658E-5 0.0 7.42996067958666E-5 0.0 76-77 6.95232035018466E-4 1.0614229542266658E-5 0.0 7.960672156699994E-5 0.0 78-79 8.013743304411327E-4 1.0614229542266658E-5 0.0 9.02209511092666E-5 0.0 80-81 8.54445478152466E-4 1.0614229542266658E-5 0.0 9.552806588039993E-5 0.0 82-83 0.0010083518065153326 1.0614229542266658E-5 0.0 9.552806588039993E-5 0.0 84-85 0.0011781794791915988 1.0614229542266658E-5 0.0 1.1675652496493324E-4 0.0 86-87 0.0014912992506884654 1.0614229542266658E-5 0.0 1.1675652496493324E-4 0.0 88-89 0.001841568825583265 1.0614229542266658E-5 0.0 1.273707545071999E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 244450 0.0 91.359604 5 GTATCAA 245885 0.0 91.08374 9 GGTATCA 246170 0.0 90.96816 8 TGGTATC 246315 0.0 90.79513 7 AAGCAGT 246930 0.0 90.79121 1 GTGGTAT 246960 0.0 90.5897 6 GCAGTGG 247330 0.0 90.447975 3 AGCAGTG 248675 0.0 90.10922 2 CAGTGGT 248315 0.0 89.959114 4 TCAACGC 244695 0.0 46.243965 12-13 ATCAACG 244350 0.0 46.200714 10-11 CAACGCA 244760 0.0 46.13568 12-13 AACGCAG 245565 0.0 46.081802 14-15 ACGCAGA 245515 0.0 46.00032 14-15 CGCAGAG 246025 0.0 45.975098 16-17 TATCAAC 246595 0.0 45.822453 10-11 CAGAGTA 245765 0.0 45.580265 18-19 AGAGTAC 241340 0.0 45.543777 18-19 GAGTACT 139765 0.0 45.351204 20-21 GCAGAGT 248365 0.0 45.325035 16-17 >>END_MODULE