FastQCFastQC Report
Sat 18 Jun 2016
SRR578575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9421315
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130970.13901456431506642No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98830.10490043056622138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3250.026.26463
ATCAACG74300.019.78069710-11
CAACGCA74500.019.66395812-13
ACTCGCG2450.018.38341550-51
TCGCGTA2400.017.778752-53
CGCGTAG2550.017.66249854-55
CGTACGC2450.017.41725520-21
CGTAGCG2600.017.32283456-57
TCAACGC73100.017.21931510-11
GTATCAA96850.016.4987879
ACGCAGA90050.016.3999714-15
CTCGCGT2750.016.37795452-53
AGTGGTA96850.016.2550325
AACGCAG78350.016.18652212-13
GGTATCA98750.015.9419388
AAGCAGT110150.015.457611
ACGTACG2901.8189894E-1214.71457820-21
TGGTATC109550.014.5434377
AGAGTAC102400.014.26048918-19
TATCAAC99000.013.745759
Produced by FastQC (version 0.11.5)