FastQCFastQC Report
Sat 18 Jun 2016
SRR578574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7490161
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92059912.290777194241887No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT191940.2562561739327099No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG86860.11596546456077512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2015800.091.999191
AGTGGTA2009900.091.791745
GGTATCA2012800.091.719448
GTATCAA2012550.091.665869
TGGTATC2013850.091.637737
GTGGTAT2021450.091.392636
AGCAGTG2040350.090.915352
GCAGTGG2044050.090.59323
CAGTGGT2034950.090.557614
TCAACGC2007450.046.3959712-13
CAACGCA2009250.046.35558712-13
ATCAACG2009800.046.3293910-11
AACGCAG2019950.046.1806314-15
ACGCAGA2024550.046.10855514-15
TATCAAC2018050.046.02227810-11
CGCAGAG2036350.045.88610516-17
AGAGTAC1984000.045.87175818-19
CAGAGTA2021300.045.70673418-19
GCAGAGT2046350.045.44135716-17
GAGTACT1199650.045.36078620-21
Produced by FastQC (version 0.11.5)