FastQCFastQC Report
Sat 18 Jun 2016
SRR578574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7490161
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA899901.2014427994271417No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97230.12981029379742304No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85940.11473718655713809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG6050.037.6856353
CTCGCGT3850.027.7606252-53
CGCGTAG4300.027.06399754-55
ACGTACG4450.026.15024420-21
CGTACGC4450.025.61656620-21
TCGCGTA4750.023.00072952-53
ACTCGCG4900.022.78209550-51
CGTAGCG5100.020.95556856-57
ACGTGTA11450.020.7412177
CGCTAGT5550.019.68406190-91
GTCACGT12000.019.3956724
CAACGCA56800.019.35852812-13
CGTGTAC11800.019.320978
ATCAACG59050.018.58068710-11
CGCGGTA3600.018.47194536-37
GGTATCA71350.017.5743668
AAGCAGT77250.017.3645841
CGCATAC6850.017.33608424-25
ACGCATA6850.016.98936324-25
GTATCAA74450.016.7787939
Produced by FastQC (version 0.11.5)