FastQCFastQC Report
Sat 18 Jun 2016
SRR578573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7515278
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92974512.371398636218114No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT191810.2552267527561855No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG88980.11839881372319161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2014000.091.952281
AGTGGTA2006550.091.748265
GGTATCA2011850.091.7457358
GTATCAA2013350.091.6742559
TGGTATC2013050.091.522547
GTGGTAT2019100.091.3896
AGCAGTG2036200.090.883792
GCAGTGG2042600.090.5227663
CAGTGGT2037600.090.420664
TCAACGC2009100.046.38160312-13
CAACGCA2009650.046.3358112-13
ATCAACG2009000.046.29892310-11
AACGCAG2019850.046.2208614-15
ACGCAGA2024750.046.06089814-15
TATCAAC2018550.045.99984410-11
CGCAGAG2031450.045.95726816-17
AGAGTAC1979050.045.79593318-19
CAGAGTA2021500.045.62030418-19
GCAGAGT2046200.045.3787316-17
GAGTACT1203850.045.35716220-21
Produced by FastQC (version 0.11.5)