FastQCFastQC Report
Sat 18 Jun 2016
SRR578573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7515278
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96780.1287776712983871No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85510.11378155272499567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG5850.035.7077983
CGCGTAG3450.030.96400354-55
CGTACGC3600.029.67235820-21
ACGTACG3850.026.51244220-21
ACTCGCG4450.024.54244650-51
CGCTAGT4200.024.30501490-91
TCGCGTA4400.024.28093552-53
CTCGCGT4400.023.7413652-53
CGTAGCG5150.020.74177256-57
ACGTGTA10900.020.0356457
CAACGCA53600.019.04332512-13
GTCACGT11750.018.5862584
ATCAACG56150.018.1784910-11
CGTGTAC12200.017.90078
GGTATCA68650.017.2199088
GTGTACG12950.016.8639819
ACGCTAG6200.016.84758690-91
AGCGACG6350.016.82234658-59
TCAACGC52300.016.7934210-11
GTATCAA70650.016.530849
Produced by FastQC (version 0.11.5)