##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7515278 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.341515244013596 31.0 31.0 34.0 30.0 34.0 2 31.71798169009849 31.0 31.0 34.0 30.0 34.0 3 31.794843783556644 31.0 31.0 34.0 30.0 34.0 4 35.50652923285073 37.0 35.0 37.0 33.0 37.0 5 35.324011566837584 37.0 35.0 37.0 33.0 37.0 6 35.315100918422445 37.0 35.0 37.0 32.0 37.0 7 35.30676217167216 37.0 35.0 37.0 32.0 37.0 8 35.26210753614171 37.0 35.0 37.0 32.0 37.0 9 36.790842867023684 39.0 37.0 39.0 32.0 39.0 10-11 36.78433359351443 39.0 37.0 39.0 32.0 39.0 12-13 36.77216098991947 39.0 37.0 39.0 32.0 39.0 14-15 37.92550388688216 40.0 37.0 41.0 33.0 41.0 16-17 37.8560474409596 40.0 37.0 41.0 32.0 41.0 18-19 37.7873923625979 40.0 37.0 41.0 32.0 41.0 20-21 37.74344781390655 40.0 37.0 41.0 32.0 41.0 22-23 37.53353861294286 39.0 36.0 41.0 32.0 41.0 24-25 37.53283231837864 39.0 36.0 41.0 32.0 41.0 26-27 37.348202222193244 39.0 36.0 41.0 31.5 41.0 28-29 37.18605352988938 39.0 36.0 41.0 31.0 41.0 30-31 37.038656188101086 39.0 36.0 40.5 31.0 41.0 32-33 36.888108796507595 39.0 36.0 40.0 30.0 41.0 34-35 36.72712526402882 39.0 35.0 40.0 30.0 41.0 36-37 36.566320500718675 38.0 35.0 40.0 30.0 41.0 38-39 36.38134870060695 38.0 35.0 40.0 30.0 41.0 40-41 36.20506313938087 38.0 35.0 40.0 30.0 41.0 42-43 36.2765587646924 38.0 35.0 40.0 30.0 41.0 44-45 36.19585363309248 38.0 35.0 40.0 30.0 41.0 46-47 36.02727517198964 38.0 35.0 40.0 29.0 41.0 48-49 35.85684860892704 38.0 34.0 40.0 28.5 41.0 50-51 35.643212000939954 38.0 34.0 40.0 28.0 41.0 52-53 35.679008680716805 38.0 34.0 40.0 28.0 41.0 54-55 35.651531586722406 38.0 34.0 40.0 28.0 41.0 56-57 35.47025000272778 38.0 34.0 40.0 28.0 41.0 58-59 35.218908668448464 38.0 34.0 40.0 27.5 41.0 60-61 34.93599724454637 37.0 33.5 40.0 27.0 41.0 62-63 34.63607400817375 37.0 33.0 40.0 26.0 41.0 64-65 34.293746951210586 36.0 33.0 39.0 26.0 41.0 66-67 33.93044788230056 36.0 32.5 39.0 26.0 41.0 68-69 33.53956972716112 35.5 32.0 39.0 26.0 40.0 70-71 33.13309900179341 35.0 32.0 38.0 25.0 40.0 72-73 32.731197169286354 35.0 31.0 37.5 24.5 40.0 74-75 32.3170099629049 35.0 31.0 37.0 24.0 39.0 76-77 30.959994094696164 33.5 29.5 35.5 21.5 38.5 78-79 31.616796411257177 34.0 31.0 36.0 23.0 39.0 80-81 31.497993487399935 34.0 31.0 36.0 23.0 37.5 82-83 31.134699075669587 34.0 31.0 35.0 21.5 37.0 84-85 30.855773000014104 34.0 30.0 35.0 20.5 37.0 86-87 30.535532351564374 34.0 30.0 35.0 20.0 36.0 88-89 30.22012372662728 34.0 30.0 35.0 18.5 36.0 90-91 29.86418300161351 34.0 30.0 35.0 17.5 35.5 92-93 29.472267027247696 34.0 29.0 35.0 12.5 35.0 94-95 29.06498508771066 34.0 29.0 35.0 4.5 35.0 96-97 28.560482060676932 34.0 29.0 35.0 2.0 35.0 98-99 27.714001597811816 33.0 27.0 35.0 2.0 35.0 100-101 26.608876744147054 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3457455283035813 1101 2 0.24800022900241459 1101 3 0.33661908075544744 1101 4 0.23249481652256776 1101 5 0.245481179678535 1101 6 0.15470807459365687 1101 7 0.22666100908936926 1101 8 0.18262400301127002 1101 9 0.2842496394613505 1101 10-11 0.3009593418737211 1101 12-13 0.3047341502601739 1101 14-15 0.2524446877423472 1101 16-17 0.28564332364108935 1101 18-19 0.3574991111718546 1101 20-21 0.2974195769414365 1101 22-23 0.41308132144445153 1101 24-25 0.35140234818599225 1101 26-27 0.33255695909412 1101 28-29 0.3112008806275739 1101 30-31 0.36670855265342794 1101 32-33 0.424280558690981 1101 34-35 0.4625962457754227 1101 36-37 0.44838316104222997 1101 38-39 0.2666132144278919 1101 40-41 0.3278946050419336 1101 42-43 0.33144287062727074 1101 44-45 0.2650163890452504 1101 46-47 0.17378278988280726 1101 48-49 0.22595374420062342 1101 50-51 0.34518436499956806 1101 52-53 0.37072050531056533 1101 54-55 0.5002755763496438 1101 56-57 0.5714369045701844 1101 58-59 0.4340650785619502 1101 60-61 0.44056826423403095 1101 62-63 0.34937529158215597 1101 64-65 0.41416688020044745 1101 66-67 0.5023323351472584 1101 68-69 0.33390237422818103 1101 70-71 0.29360502938875044 1101 72-73 0.42232932687893054 1101 74-75 0.426963413181376 1101 76-77 0.2687977465505682 1101 78-79 0.36436511420302153 1101 80-81 0.3322439732185707 1101 82-83 0.5198337207333132 1101 84-85 0.40774634927869613 1101 86-87 0.44523510553104373 1101 88-89 0.5150542940683849 1101 90-91 0.4384186273708188 1101 92-93 0.4657484274881831 1101 94-95 0.31612094764019005 1101 96-97 0.39650988405687215 1101 98-99 0.7801790214790607 1101 100-101 0.9747025625032926 1102 1 0.26507937142215 1102 2 0.25478763960399675 1102 3 0.2859210987479983 1102 4 0.2683692132165092 1102 5 0.28885874688445057 1102 6 0.2135464420145894 1102 7 0.2356108125537446 1102 8 0.13170864490378165 1102 9 0.2229551884207197 1102 10-11 0.2735954691623803 1102 12-13 0.31709939663534215 1102 14-15 0.3636609249980083 1102 16-17 0.2217939461040359 1102 18-19 0.2908547114076896 1102 20-21 0.3232154539260108 1102 22-23 0.4096218290812246 1102 24-25 0.3266162381126563 1102 26-27 0.38075684697066237 1102 28-29 0.37062081233818134 1102 30-31 0.420575864883844 1102 32-33 0.3007554746707939 1102 34-35 0.29502351524433124 1102 36-37 0.35010181704807763 1102 38-39 0.3076646659526361 1102 40-41 0.26956611440203915 1102 42-43 0.20663743139123625 1102 44-45 0.27249483427417687 1102 46-47 0.33663547137012984 1102 48-49 0.17280982334629869 1102 50-51 0.21899554159980283 1102 52-53 0.31670657240135114 1102 54-55 0.3308120732142896 1102 56-57 0.38827668483590116 1102 58-59 0.4950772485942636 1102 60-61 0.532047417383346 1102 62-63 0.2865390930620464 1102 64-65 0.244469023658624 1102 66-67 0.33980059132922236 1102 68-69 0.31244495635864666 1102 70-71 0.3485931267989386 1102 72-73 0.5187105357836472 1102 74-75 0.39123503796718495 1102 76-77 0.22092066051813575 1102 78-79 0.3221043208721319 1102 80-81 0.5030718276255861 1102 82-83 0.46488914847638085 1102 84-85 0.3641730770050806 1102 86-87 0.43090316303857534 1102 88-89 0.38689035247577763 1102 90-91 0.4756367778690489 1102 92-93 0.47762562623661964 1102 94-95 0.5557959989990344 1102 96-97 0.6799829239777431 1102 98-99 1.0098961669041024 1102 100-101 1.005011282171047 1103 1 0.24008445882909513 1103 2 0.23938740388733848 1103 3 0.2714230075011983 1103 4 0.22382331024353874 1103 5 0.22073276794619545 1103 6 0.07567868736850158 1103 7 0.21255319696039265 1103 8 0.07347817765443665 1103 9 0.20129933608325956 1103 10-11 0.22669569658504685 1103 12-13 0.18567784513763286 1103 14-15 0.3336796745694315 1103 16-17 0.27348689294792194 1103 18-19 0.25364670880898643 1103 20-21 0.3794602821516406 1103 22-23 0.36087033364047016 1103 24-25 0.3208416201312261 1103 26-27 0.17686750084099856 1103 28-29 0.25270344040073667 1103 30-31 0.3107728518005004 1103 32-33 0.2209438892842499 1103 34-35 0.1858326272110844 1103 36-37 0.28197695891829966 1103 38-39 0.33380695918274483 1103 40-41 0.22102744985565437 1103 42-43 0.26753335300152514 1103 44-45 0.21415128808491346 1103 46-47 0.3307445347396154 1103 48-49 0.3206680720639099 1103 50-51 0.26367287368359626 1103 52-53 0.16324747192165745 1103 54-55 0.3751590285920514 1103 56-57 0.32775300253729256 1103 58-59 0.21984819747415685 1103 60-61 0.21908455722933695 1103 62-63 0.27696324085941626 1103 64-65 0.18669091307435792 1103 66-67 0.20759017064758467 1103 68-69 0.3461161619069557 1103 70-71 0.24796947787424983 1103 72-73 0.17144223974288764 1103 74-75 0.2996554626187802 1103 76-77 0.17968258242291668 1103 78-79 0.30652304595265534 1103 80-81 0.28432443074418146 1103 82-83 0.4563116175644417 1103 84-85 0.2713356436643437 1103 86-87 0.15470050568184845 1103 88-89 0.32952186057496036 1103 90-91 0.365788376132933 1103 92-93 0.2874737432710148 1103 94-95 0.4807468842716567 1103 96-97 0.4551767228078738 1103 98-99 0.975301636060049 1103 100-101 0.852169133580869 1104 1 0.19285511266989275 1104 2 0.2104719097608232 1104 3 0.10878902888970643 1104 4 0.18447908076532116 1104 5 0.1909339709912956 1104 6 0.11905848272787978 1104 7 0.11055675104384477 1104 8 0.14078541992171978 1104 9 0.22243420074890707 1104 10-11 0.10726121437298985 1104 12-13 0.15544584049971633 1104 14-15 0.2861519199934648 1104 16-17 0.29328596408689833 1104 18-19 0.10709420360138466 1104 20-21 0.21262963474705288 1104 22-23 0.31224873638506523 1104 24-25 0.22952309450914754 1104 26-27 0.32886829986463795 1104 28-29 0.2649727668364008 1104 30-31 0.21604894884357861 1104 32-33 0.26285328950357467 1104 34-35 0.19774209099556828 1104 36-37 0.2038621123469042 1104 38-39 0.21587766692426413 1104 40-41 0.2797788206179206 1104 42-43 0.20312476141968006 1104 44-45 0.1276077003429137 1104 46-47 0.21689247359004327 1104 48-49 0.21881343813792142 1104 50-51 0.18328697967335472 1104 52-53 0.08863176130672201 1104 54-55 0.14621545668757818 1104 56-57 0.12739512115862084 1104 58-59 0.145615925546295 1104 60-61 0.23669172904997282 1104 62-63 0.16201167625184354 1104 64-65 0.16258780019008867 1104 66-67 0.21420320918527835 1104 68-69 0.258475877377883 1104 70-71 0.22069284239309894 1104 72-73 0.29610144664187743 1104 74-75 0.253235433860759 1104 76-77 0.16509674047919276 1104 78-79 0.3284276974428657 1104 80-81 0.35218679299277156 1104 82-83 0.4474795222022685 1104 84-85 0.3436491093093643 1104 86-87 0.4272316535625116 1104 88-89 0.43256362143508653 1104 90-91 0.34585380998955273 1104 92-93 0.44679875962401994 1104 94-95 0.4573906462897419 1104 96-97 0.4885490759827711 1104 98-99 0.8665034721636253 1104 100-101 0.9674591194548547 1105 1 0.24100407097458287 1105 2 0.1651431855521004 1105 3 0.12609757180945635 1105 4 0.14229492181097214 1105 5 0.18635461780047535 1105 6 0.13573027503841928 1105 7 0.24049885418121875 1105 8 0.05464288533884343 1105 9 0.19797921394626883 1105 10-11 0.10591899719710085 1105 12-13 0.13653686000755272 1105 14-15 0.1339952354947016 1105 16-17 0.10336339897489921 1105 18-19 0.1905277349546921 1105 20-21 0.13058167510486385 1105 22-23 0.21870306768283143 1105 24-25 0.2294056252978507 1105 26-27 0.19291976917612885 1105 28-29 0.20864958359623387 1105 30-31 0.1223644906997805 1105 32-33 0.26965281863487434 1105 34-35 0.20383664007262325 1105 36-37 0.15829911670844155 1105 38-39 0.1978573406232087 1105 40-41 0.18269239290858508 1105 42-43 0.2193525562076033 1105 44-45 0.23937284371718448 1105 46-47 0.1986680474769713 1105 48-49 0.1961052899147262 1105 50-51 0.2550824274361716 1105 52-53 0.2204966949721694 1105 54-55 0.28091131202853603 1105 56-57 0.2195193260874575 1105 58-59 0.15735467012827797 1105 60-61 0.029785273902582787 1105 62-63 0.10883115929817677 1105 64-65 0.16190309671846848 1105 66-67 0.29234534501628673 1105 68-69 0.32838964414796123 1105 70-71 0.36761962811029747 1105 72-73 0.38881234632828665 1105 74-75 0.4510532090287356 1105 76-77 0.2456945635024148 1105 78-79 0.3921429034862207 1105 80-81 0.479595547429291 1105 82-83 0.5536533743750596 1105 84-85 0.5027393100324637 1105 86-87 0.5093889607971818 1105 88-89 0.525009991757301 1105 90-91 0.6485652897243313 1105 92-93 0.7858223737447112 1105 94-95 0.8042580884760575 1105 96-97 0.8391203530044571 1105 98-99 1.248107996126592 1105 100-101 1.339296168738752 1106 1 0.1443000418275595 1106 2 0.16104846398846817 1106 3 0.13275502446991183 1106 4 0.08821925940352315 1106 5 0.05758171868453843 1106 6 0.015307486984191598 1106 7 0.05319230027407684 1106 8 0.035401147730020455 1106 9 0.15048208150444964 1106 10-11 -0.025198180455369368 1106 12-13 0.07410205284419646 1106 14-15 -0.02611253236724309 1106 16-17 0.02675487295726242 1106 18-19 0.07786483593041993 1106 20-21 0.11554911170023274 1106 22-23 0.1765226868784282 1106 24-25 0.08557223974485595 1106 26-27 0.056607744355225975 1106 28-29 0.1042692096434763 1106 30-31 0.17921538150499572 1106 32-33 0.16081857755614237 1106 34-35 0.09469980513260623 1106 36-37 0.013308472281302386 1106 38-39 0.025082309648489343 1106 40-41 0.09686634283934126 1106 42-43 0.16082286383505817 1106 44-45 0.06841086353514214 1106 46-47 0.04472622952332728 1106 48-49 0.12155705100354908 1106 50-51 0.14534656939751756 1106 52-53 0.11966056105148226 1106 54-55 0.040695884553898054 1106 56-57 0.10946901019428878 1106 58-59 0.043583891255259744 1106 60-61 0.20205751194595933 1106 62-63 0.16776885672413044 1106 64-65 0.08607889696691728 1106 66-67 0.07388792281759748 1106 68-69 0.19630403121553286 1106 70-71 0.21040610078211586 1106 72-73 0.23890502272558933 1106 74-75 0.3472384606459613 1106 76-77 0.16901293822715147 1106 78-79 0.35501588591530364 1106 80-81 0.39757241121571596 1106 82-83 0.5083531278009588 1106 84-85 0.5156465592409454 1106 86-87 0.5714554413733666 1106 88-89 0.6263763686328794 1106 90-91 0.6469551519870933 1106 92-93 0.624248301616376 1106 94-95 0.6742284590447092 1106 96-97 0.6857396554050688 1106 98-99 1.1631090447329981 1106 100-101 1.209044333693047 1107 1 0.15491913979420957 1107 2 0.05839902119566531 1107 3 -0.015376718624999342 1107 4 -0.008496750050390744 1107 5 -0.011156546500529885 1107 6 0.017009095277451536 1107 7 0.03134594097612364 1107 8 0.031137544751331347 1107 9 0.03374945590228151 1107 10-11 -0.036477911261918905 1107 12-13 0.00635246924812094 1107 14-15 -0.03758381646175479 1107 16-17 0.016814495750601566 1107 18-19 0.016221799819348348 1107 20-21 0.03193852951719123 1107 22-23 0.1989557468380596 1107 24-25 0.12031272825522166 1107 26-27 0.09119604695456474 1107 28-29 0.135619682923938 1107 30-31 0.10184343672572993 1107 32-33 0.09554686340129592 1107 34-35 0.15405503621560257 1107 36-37 0.08840814214202197 1107 38-39 0.21412429338313643 1107 40-41 0.13852312057769467 1107 42-43 0.0715327358263309 1107 44-45 0.10604278509651266 1107 46-47 0.1035239800671306 1107 48-49 0.11526696724877183 1107 50-51 0.18968004626346868 1107 52-53 0.1512042673760945 1107 54-55 0.11258072248506323 1107 56-57 0.26025294324690407 1107 58-59 0.18210839097270792 1107 60-61 0.27324535795091975 1107 62-63 0.33585477484847104 1107 64-65 0.3385041365925332 1107 66-67 0.22347103634465526 1107 68-69 0.316420372961872 1107 70-71 0.3270002152157261 1107 72-73 0.2876439350749038 1107 74-75 0.3184465767270126 1107 76-77 0.2069744814002341 1107 78-79 0.2911423320975821 1107 80-81 0.35601070004258517 1107 82-83 0.4636699791425656 1107 84-85 0.49447466786571326 1107 86-87 0.5128504733936445 1107 88-89 0.4707402613023213 1107 90-91 0.6298922725740717 1107 92-93 0.6557568628603043 1107 94-95 0.6688779163750382 1107 96-97 0.6237015482728445 1107 98-99 1.1758412988952607 1107 100-101 1.2367658693310446 1108 1 0.033811950537430135 1108 2 0.024764485855655494 1108 3 -0.028428307563530097 1108 4 0.045098908022879414 1108 5 0.04425753506401975 1108 6 0.05769910710095871 1108 7 -0.06178542188964542 1108 8 0.08361063431565441 1108 9 0.021935654181852726 1108 10-11 -0.008881554032619476 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2316 84-85 -1.477296836636583 2316 86-87 -1.5360931232535826 2316 88-89 -1.5102980232534087 2316 90-91 -1.4383375991310459 2316 92-93 -1.459061051282049 2316 94-95 -1.640439647183488 2316 96-97 -1.789256840772353 2316 98-99 -1.3936186921078964 2316 100-101 -1.6831742839636696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124.0 3 77.0 4 159.0 5 489.0 6 851.0 7 1981.0 8 3607.0 9 5488.0 10 7708.0 11 9807.0 12 12598.0 13 15094.0 14 17765.0 15 20165.0 16 23023.0 17 27213.0 18 31326.0 19 35979.0 20 38316.0 21 40630.0 22 45227.0 23 53512.0 24 65753.0 25 83059.0 26 105011.0 27 132134.0 28 164340.0 29 202568.0 30 246266.0 31 296691.0 32 361114.0 33 446470.0 34 571104.0 35 780554.0 36 1004582.0 37 1328645.0 38 1116181.0 39 219292.0 40 375.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.78842834932607 30.03400761286495 28.740430944342286 23.437133093466695 2 22.88660512625082 29.02202686314465 27.328742862206827 20.762625148397703 3 21.914545276967797 27.92977185940427 27.822869626379752 22.332813237248175 4 22.23431472437855 27.97405583897311 27.80440005923981 21.98722937740853 5 22.758826249380423 27.97752562298534 28.04343183337824 21.220216294255994 6 22.728808387932148 27.986062515626514 27.050208289173263 22.23492080726808 7 22.957410411663513 28.10579907771703 27.48802969529746 21.44876081532199 8 22.145528082926543 28.19798549035711 27.487512770651996 22.168973656064352 9 21.810599435708745 27.914799578671044 28.06760850045534 22.206992485164868 10-11 21.897409817170686 28.352621419269404 27.45100269970664 22.29896606385327 12-13 21.91770550603717 28.428196535111542 27.307519429088316 22.34657852976297 14-15 22.081438637399707 28.482858518340905 27.132096776726023 22.303606067533362 16-17 22.24212390868945 28.255003542118747 27.160858762871783 22.342013786320024 18-19 22.19117804972458 28.274433530527215 27.13368382828737 22.400704591460833 20-21 22.282296143935064 28.19616253716762 27.191602226823814 22.329939092073506 22-23 22.356398625670987 28.15469677512241 27.160535717398442 22.32836888180816 24-25 22.230210394759208 28.130218744417622 27.14114681726656 22.498424043556607 26-27 22.439708396255252 28.01552493078423 27.232159549354666 22.312607123605854 28-29 22.423901159733177 28.088365781164505 27.192776897740362 22.294956161361956 30-31 22.377765634623486 28.075346047184823 27.17467825427867 22.372210063913016 32-33 22.435660994215777 28.119269167974004 27.060777590159766 22.38429224765045 34-35 22.4527698706521 28.133518779779244 27.023893916294284 22.389817433274377 36-37 22.440077014066265 28.126201515172383 27.011202088340237 22.422519382421118 38-39 22.469379210510333 28.10798094234795 27.02383763352029 22.398802213621426 40-41 22.450389146350407 28.155742105929193 27.01498288964302 22.378885858077382 42-43 22.46129043074858 28.126651653529727 26.990327331449503 22.421730584272186 44-45 22.444939498216492 28.141689020154132 27.00584931964185 22.407522161987526 46-47 22.492805800152915 28.18100938837341 26.941336090114653 22.38484872135902 48-49 22.52301094259863 28.152476180628668 26.897981083965206 22.4265317928075 50-51 22.516549329277257 28.14091265666887 26.933432073727936 22.409105940325937 52-53 22.472257175006206 28.20322580001211 26.88042022303388 22.444096801947804 54-55 22.511611430946935 28.203518047669444 26.79015909021047 22.494711431173155 56-57 22.52028136263612 28.21699319879511 26.80766511924979 22.45506031931898 58-59 22.499904526609665 28.342616540408926 26.742429292806307 22.4150496401751 60-61 22.516958121841935 28.281042963413995 26.756002905015624 22.44599600972845 62-63 22.490293430536305 28.310436177218364 26.755615333099765 22.443655059145573 64-65 22.531745434544817 28.338825192547727 26.750081451434287 22.379347921473165 66-67 22.49602388761166 28.356499184817952 26.74411866362705 22.40335826394334 68-69 22.47619043817152 28.378215282191142 26.745557713004626 22.400036566632707 70-71 22.477641099393118 28.443620654232372 26.691953882887958 22.386784363486555 72-73 22.490709362016908 28.39120096374858 26.685272560644474 22.432817113590033 74-75 22.50338667449622 28.44772645210842 26.666070534777543 22.38281633861782 76-77 22.471829974814938 28.479605714788313 26.697104216818012 22.351460093578734 78-79 22.473019854810733 28.48984771573604 26.663026023678015 22.37410640577521 80-81 22.45168429347336 28.502667506557994 26.671585992124292 22.37406220784436 82-83 22.488462240125124 28.572833360855675 26.61077879529859 22.327925603720615 84-85 22.436360936407638 28.568352930312322 26.630579270962045 22.36470686231799 86-87 22.43908254934812 28.608051837832726 26.595118992634724 22.35774662018443 88-89 22.428999167560846 28.695532186227922 26.55955266508269 22.31591598112854 90-91 22.424127908814107 28.652207743043345 26.54870347718739 22.374960870955153 92-93 22.43902231314354 28.681924167486894 26.545764052881328 22.33328946648824 94-95 22.391767307587006 28.772129209721857 26.512797349534612 22.323306133156517 96-97 22.410232963766912 28.765949502628207 26.495239050761505 22.32857848284338 98-99 22.439751420513453 28.762216870538698 26.437603493404914 22.36042821554293 100-101 22.48527878903482 28.73480184288996 26.401163818451295 22.37875554962392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98567.0 1 56120.0 2 9775.0 3 5247.5 4 4349.5 5 3826.5 6 3537.5 7 3550.5 8 3332.5 9 2993.5 10 3074.5 11 3600.0 12 6316.5 13 6263.0 14 3983.0 15 4227.0 16 4681.5 17 5433.5 18 6592.0 19 7908.5 20 9899.0 21 13081.5 22 17362.0 23 22475.5 24 27922.0 25 34612.0 26 43930.0 27 55093.5 28 65344.0 29 75589.0 30 88284.5 31 102343.0 32 116473.5 33 129810.0 34 142405.0 35 155704.5 36 171847.5 37 187177.0 38 197326.0 39 204414.5 40 212015.0 41 221636.5 42 230108.0 43 237895.0 44 247765.0 45 257742.0 46 265574.0 47 271604.5 48 276444.5 49 279881.5 50 281046.5 51 279312.0 52 278997.5 53 276335.5 54 263536.0 55 246804.5 56 228443.0 57 206666.0 58 185948.5 59 163484.0 60 137611.0 61 113055.0 62 89110.0 63 65714.5 64 47249.0 65 33246.5 66 22618.5 67 16176.0 68 10705.0 69 6340.0 70 3605.5 71 1652.5 72 775.0 73 382.5 74 199.0 75 113.5 76 65.0 77 49.0 78 46.5 79 38.0 80 26.0 81 15.5 82 13.0 83 10.5 84 8.5 85 6.5 86 3.5 87 2.5 88 3.0 89 2.5 90 2.5 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15520383943215407 2 0.0 3 0.0 4 8.116798872909291E-4 5 7.05230066006873E-4 6 6.653113830253519E-5 7 1.862871872470985E-4 8 0.0 9 1.3306227660507037E-4 10-11 2.2620587022861963E-4 12-13 0.0 14-15 0.0 16-17 2.6612455321014072E-5 18-19 1.3306227660507036E-5 20-21 0.0 22-23 6.253927000438307E-4 24-25 6.653113830253518E-6 26-27 1.463685042655774E-4 28-29 0.005934577536586138 30-31 0.0039719089566613505 32-33 1.0644982128405629E-4 34-35 0.0010711513266708165 36-37 3.659212606639435E-4 38-39 0.0010844575543313235 40-41 0.0031801884108611815 42-43 0.001091110668161577 44-45 5.987802447228167E-4 46-47 0.009547218346413798 48-49 0.009327665590015433 50-51 0.008289779832495884 52-53 0.015608205045774754 54-55 0.006453520415345913 56-57 8.715579117632108E-4 58-59 0.0012640916277481685 60-61 0.0 62-63 9.314359362354925E-5 64-65 8.449454564421968E-4 66-67 0.0011376824649733516 68-69 0.0030604323619166183 70-71 0.0019959341490760553 72-73 0.0060543335855307015 74-75 0.002162261994832393 76-77 0.0014503788149952668 78-79 0.0016233597745818583 80-81 2.6612455321014074E-4 82-83 0.002275364929946703 84-85 5.3224910642028145E-5 86-87 0.0019826279214155487 88-89 1.862871872470985E-4 90-91 0.0010711513266708165 92-93 0.001476991270316281 94-95 9.247828224052389E-4 96-97 9.979670745380277E-4 98-99 0.5344313277566046 100-101 2.1955275639836612E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7515278.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.18694387932877 #Duplication Level Percentage of deduplicated Percentage of total 1 90.83303661387666 69.20291462890437 2 5.271075569163734 8.031742771431565 3 1.3409443258946678 3.064873503067242 4 0.6145014782505923 1.872679585489698 5 0.4201639802616644 1.6005504792155412 6 0.2780675754993454 1.2711071261537787 7 0.1988562081489557 1.0605172739210424 8 0.15838741878954563 0.9653642709208684 9 0.12309436341717801 0.8440365021773618 >10 0.7407481319294262 9.987146098412687 >50 0.02044184042910186 0.9662809779596431 >100 3.908275508939817E-4 0.052651226277727385 >500 4.8611131369484016E-5 0.02088792915182462 >1k 1.4583339408639814E-4 0.384130498457379 >5k 9.722226272426542E-5 0.6751171284592713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9678 0.1287776712983871 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8551 0.11378155272499567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 2.6612455321014072E-5 0.0 0.0 0.0 1.3306227660507036E-5 4 2.6612455321014072E-5 0.0 0.0 0.0 6.653113830253519E-5 5 2.6612455321014072E-5 0.0 0.0 0.0 7.983736596304222E-5 6 3.991868298152111E-5 0.0 0.0 1.3306227660507036E-5 9.314359362354925E-5 7 5.3224910642028145E-5 0.0 0.0 2.6612455321014072E-5 1.0644982128405629E-4 8 6.653113830253519E-5 0.0 0.0 3.991868298152111E-5 1.0644982128405629E-4 9 6.653113830253519E-5 0.0 0.0 3.991868298152111E-5 1.1975604894456332E-4 10-11 7.983736596304222E-5 0.0 0.0 3.991868298152111E-5 1.1975604894456332E-4 12-13 7.983736596304222E-5 0.0 0.0 5.987802447228166E-5 1.6632784575633798E-4 14-15 9.314359362354925E-5 0.0 0.0 6.653113830253519E-5 2.1289964256811258E-4 16-17 1.0644982128405629E-4 0.0 0.0 6.653113830253519E-5 2.461652117193802E-4 18-19 1.2640916277481683E-4 0.0 0.0 6.653113830253519E-5 2.860838947009013E-4 20-21 1.5967473192608444E-4 6.653113830253518E-6 0.0 6.653113830253519E-5 3.260025776824224E-4 22-23 1.5967473192608444E-4 1.3306227660507036E-5 0.0 7.983736596304222E-5 3.45961919173183E-4 24-25 1.729809595865915E-4 1.3306227660507036E-5 0.0 7.983736596304222E-5 3.72574374494197E-4 26-27 1.9294030107735202E-4 1.3306227660507036E-5 0.0 7.983736596304222E-5 3.72574374494197E-4 28-29 1.9959341490760556E-4 1.3306227660507036E-5 0.0 7.983736596304222E-5 3.8588060215470404E-4 30-31 1.9959341490760556E-4 1.3306227660507036E-5 0.0 1.1975604894456332E-4 4.058399436454646E-4 32-33 2.128996425681126E-4 1.9959341490760555E-5 0.0 1.3306227660507037E-4 4.989835372690139E-4 34-35 2.3285898405887314E-4 2.6612455321014072E-5 0.0 1.3306227660507037E-4 5.322491064202815E-4 36-37 2.4616521171938016E-4 2.6612455321014072E-5 0.0 1.3306227660507037E-4 5.854740170623096E-4 38-39 2.5947143937988723E-4 2.6612455321014072E-5 0.0 1.463685042655774E-4 6.187395862135772E-4 40-41 2.6612455321014074E-4 2.6612455321014072E-5 0.0 1.530216180958309E-4 6.586582691950983E-4 42-43 2.927370085311548E-4 2.6612455321014072E-5 0.0 1.5967473192608444E-4 6.985769521766195E-4 44-45 2.993901223614083E-4 2.6612455321014072E-5 0.0 1.5967473192608444E-4 7.31842521327887E-4 46-47 3.326556915126759E-4 2.6612455321014072E-5 0.0 1.5967473192608444E-4 7.45148748988394E-4 48-49 3.8588060215470404E-4 2.6612455321014072E-5 0.0 1.5967473192608444E-4 7.584549766489011E-4 50-51 4.1914617130597165E-4 2.6612455321014072E-5 0.0 1.6632784575633795E-4 8.449454564421968E-4 52-53 4.4575862662698574E-4 2.6612455321014072E-5 0.0 1.79634073416845E-4 8.715579117632108E-4 54-55 4.723710819479998E-4 2.6612455321014072E-5 0.0 1.9959341490760556E-4 8.915172532539715E-4 56-57 5.056366510992674E-4 2.6612455321014072E-5 0.0 1.9959341490760556E-4 8.98170367084225E-4 58-59 5.588615617412955E-4 2.6612455321014072E-5 0.0 1.9959341490760556E-4 9.181297085749855E-4 60-61 5.65514675571549E-4 2.6612455321014072E-5 0.0 2.1289964256811258E-4 9.380890500657461E-4 62-63 5.987802447228166E-4 2.6612455321014072E-5 0.0 2.2620587022861963E-4 9.447421638959996E-4 64-65 6.719644968556054E-4 2.6612455321014072E-5 0.0 2.2620587022861963E-4 9.447421638959996E-4 66-67 7.05230066006873E-4 2.6612455321014072E-5 0.0 2.4616521171938016E-4 9.580483915565066E-4 68-69 7.717612043094081E-4 2.6612455321014072E-5 0.0 2.7943078087064776E-4 9.979670745380277E-4 70-71 8.782110255934644E-4 2.6612455321014072E-5 0.0 2.927370085311548E-4 0.001037885757519549 72-73 9.048234809144785E-4 2.6612455321014072E-5 0.0 2.927370085311548E-4 0.00107780444050107 74-75 9.913139607077741E-4 2.6612455321014072E-5 0.0 2.927370085311548E-4 0.001091110668161577 76-77 0.0010844575543313235 2.6612455321014072E-5 0.0 2.927370085311548E-4 0.0010977637819918305 78-79 0.001284050969238929 2.6612455321014072E-5 0.0 3.060432361916618E-4 0.0011376824649733516 80-81 0.001410460132013746 2.6612455321014072E-5 0.0 3.193494638521689E-4 0.0011842542617851262 82-83 0.0015900942054305907 2.6612455321014072E-5 0.0 3.193494638521689E-4 0.001230826058596901 84-85 0.0018695249863012386 2.6612455321014072E-5 0.0 3.260025776824224E-4 0.001244132286257408 86-87 0.0024084272065517734 3.326556915126759E-5 0.0 3.393088053429294E-4 0.0012640916277481685 88-89 0.0031735352970309284 3.991868298152111E-5 0.0 3.5926814683369E-4 0.0012773978554086755 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 585 0.0 35.707798 3 CGCGTAG 345 0.0 30.964003 54-55 CGTACGC 360 0.0 29.672358 20-21 ACGTACG 385 0.0 26.512442 20-21 ACTCGCG 445 0.0 24.542446 50-51 CGCTAGT 420 0.0 24.305014 90-91 TCGCGTA 440 0.0 24.280935 52-53 CTCGCGT 440 0.0 23.74136 52-53 CGTAGCG 515 0.0 20.741772 56-57 ACGTGTA 1090 0.0 20.035645 7 CAACGCA 5360 0.0 19.043325 12-13 GTCACGT 1175 0.0 18.586258 4 ATCAACG 5615 0.0 18.17849 10-11 CGTGTAC 1220 0.0 17.9007 8 GGTATCA 6865 0.0 17.219908 8 GTGTACG 1295 0.0 16.863981 9 ACGCTAG 620 0.0 16.847586 90-91 AGCGACG 635 0.0 16.822346 58-59 TCAACGC 5230 0.0 16.79342 10-11 GTATCAA 7065 0.0 16.53084 9 >>END_MODULE