FastQCFastQC Report
Sat 18 Jun 2016
SRR578572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7252243
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87670412.088728962887757No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT180010.24821286324796343No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG92510.12756053540952778No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA77130.10635330338489761No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC76420.10537429592472289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1941700.091.817231
AGTGGTA1938250.091.6124655
GGTATCA1946400.091.4092258
GTATCAA1947250.091.3882759
TGGTATC1946650.091.32377
GTGGTAT1953350.091.1179356
AGCAGTG1970250.090.5163962
GCAGTGG1970200.090.428223
CAGTGGT1969250.090.2311554
TCAACGC1938750.046.32480612-13
CAACGCA1938550.046.2781612-13
ATCAACG1940500.046.2050610-11
AACGCAG1947750.046.1369814-15
ACGCAGA1950150.046.05585514-15
CGCAGAG1958600.045.87032316-17
TATCAAC1953800.045.85286710-11
AGAGTAC1916750.045.6897618-19
CAGAGTA1948600.045.59449418-19
GCAGAGT1977100.045.27546316-17
GAGTACT1129600.045.2524820-21
Produced by FastQC (version 0.11.5)