##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578572_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7252243
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.796548874603346	31.0	31.0	34.0	27.0	34.0
2	31.15001345101095	33.0	31.0	34.0	27.0	34.0
3	31.287005275471326	33.0	31.0	34.0	27.0	34.0
4	34.66255694962234	37.0	35.0	37.0	32.0	37.0
5	34.68558720384852	37.0	35.0	37.0	32.0	37.0
6	34.729142969974944	37.0	35.0	37.0	32.0	37.0
7	34.64944307574912	37.0	35.0	37.0	32.0	37.0
8	34.69646066741007	37.0	35.0	37.0	32.0	37.0
9	36.175531487292965	39.0	37.0	39.0	32.0	39.0
10-11	36.044034307730726	39.0	37.0	39.0	31.0	39.0
12-13	36.107509221629776	39.0	37.0	39.0	32.0	39.0
14-15	37.32301344563331	40.0	37.0	41.0	32.0	41.0
16-17	37.301738234639956	40.0	37.0	41.0	31.0	41.0
18-19	37.221661491486145	40.0	37.0	41.0	31.0	41.0
20-21	37.15048440875464	40.0	37.0	41.0	31.0	41.0
22-23	36.87504072877867	39.5	37.0	41.0	30.5	41.0
24-25	36.82467679309698	39.5	37.0	41.0	30.0	41.0
26-27	36.705295658184646	40.0	36.5	41.0	30.0	41.0
28-29	36.54044865843574	40.0	36.0	41.0	29.5	41.0
30-31	36.3135454093306	40.0	36.0	41.0	28.0	41.0
32-33	36.01563640379949	39.0	35.5	41.0	27.0	41.0
34-35	35.83381562090515	39.0	35.0	41.0	26.5	41.0
36-37	35.5486408136076	39.0	35.0	40.5	26.0	41.0
38-39	34.67569323035646	38.0	34.0	40.0	25.0	41.0
40-41	34.4215466856254	37.0	34.0	40.0	24.5	41.0
42-43	34.097677297906316	37.0	33.0	40.0	24.0	41.0
44-45	33.78196766159104	37.0	33.0	40.0	23.0	41.0
46-47	33.60750232721104	36.5	33.0	40.0	22.5	41.0
48-49	33.6145081597514	37.0	33.0	40.0	21.0	41.0
50-51	32.72617347488219	36.0	32.0	39.5	17.0	40.5
52-53	32.307027908469145	37.0	32.0	39.5	7.0	40.5
54-55	31.89546213495604	37.0	31.0	40.0	2.0	41.0
56-57	31.34489708907989	37.0	30.5	40.0	2.0	41.0
58-59	31.022805496175458	37.0	30.0	40.0	2.0	41.0
60-61	30.75086624924179	36.0	30.0	40.0	2.0	41.0
62-63	30.410397927923817	36.0	29.0	39.5	2.0	41.0
64-65	30.115487994541827	35.0	29.0	39.0	2.0	41.0
66-67	29.779204861171916	35.0	28.5	39.0	2.0	41.0
68-69	29.447047347972205	35.0	28.0	38.5	2.0	40.5
70-71	29.153907625544264	35.0	28.0	37.5	2.0	40.0
72-73	28.832744159289753	35.0	28.0	37.0	2.0	39.5
74-75	28.46859254440316	35.0	27.0	36.5	2.0	39.0
76-77	28.101190555804596	35.0	26.0	36.0	2.0	39.0
78-79	27.761840922870345	34.5	26.0	36.0	2.0	38.5
80-81	27.445950445951688	34.0	26.0	35.0	2.0	37.0
82-83	27.192889220617676	34.0	25.0	35.0	2.0	37.0
84-85	26.932415805703144	34.0	25.0	35.0	2.0	36.0
86-87	26.68447375522304	34.0	24.5	35.0	2.0	36.0
88-89	26.42911310335299	34.0	24.0	35.0	2.0	36.0
90-91	26.194342426198347	34.0	23.0	35.0	2.0	35.0
92-93	25.913318058978444	34.0	20.0	35.0	2.0	35.0
94-95	25.618148826507884	34.0	18.5	35.0	2.0	35.0
96-97	25.330696861646803	34.0	13.5	35.0	2.0	35.0
98-99	24.999718914548232	34.0	2.0	35.0	2.0	35.0
100-101	24.125732756059058	32.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6242582061646829
1101	2	0.6671575127502294
1101	3	0.5878929362088741
1101	4	0.6285312692135179
1101	5	0.6163737122349886
1101	6	0.6001987832891231
1101	7	0.5218512481092148
1101	8	0.5978613465056952
1101	9	0.6709798196272629
1101	10-11	0.7307403357596414
1101	12-13	0.7880467917628877
1101	14-15	0.8772872886393017
1101	16-17	0.8188763570380786
1101	18-19	0.8416401892821881
1101	20-21	0.8923512116978216
1101	22-23	0.9368834826813739
1101	24-25	0.8793315625897407
1101	26-27	0.8726011238165583
1101	28-29	1.0552008454626787
1101	30-31	1.0999087544861155
1101	32-33	1.1079442872826704
1101	34-35	1.160893884172289
1101	36-37	1.121969402678232
1101	38-39	1.1622782252924964
1101	40-41	1.0907745264416135
1101	42-43	1.1540363499450237
1101	44-45	1.319483404239378
1101	46-47	1.22487131766556
1101	48-49	1.0895506237298491
1101	50-51	1.206413962030652
1101	52-53	1.1024539719261313
1101	54-55	0.9246517978537874
1101	56-57	0.9537771919526428
1101	58-59	0.9612969804723903
1101	60-61	0.8726966605998747
1101	62-63	0.8578285892285678
1101	64-65	0.9312962132716578
1101	66-67	0.9330653909861866
1101	68-69	0.8648515866688165
1101	70-71	0.9291532933664399
1101	72-73	0.879655015934862
1101	74-75	0.8604246468195065
1101	76-77	0.8514425126956731
1101	78-79	0.8737674107054367
1101	80-81	0.8602850102626647
1101	82-83	0.8996381650342649
1101	84-85	0.8943232321645027
1101	86-87	0.8716503900349934
1101	88-89	0.8597990144794423
1101	90-91	0.9498392692215276
1101	92-93	0.98478383183501
1101	94-95	1.0583816383217979
1101	96-97	1.0512612714153953
1101	98-99	1.0125067815955155
1101	100-101	1.1199041141979755
1102	1	0.444373476475846
1102	2	0.41089410396332937
1102	3	0.4599164363037893
1102	4	0.388321106243005
1102	5	0.48046534920450057
1102	6	0.5002271661686848
1102	7	0.5059839807567528
1102	8	0.4601496567577925
1102	9	0.5641189040029033
1102	10-11	0.5592370916892051
1102	12-13	0.5140376683555914
1102	14-15	0.6972605130399714
1102	16-17	0.7090253731712366
1102	18-19	0.7877486698061418
1102	20-21	0.7739288865589415
1102	22-23	0.7645065143981356
1102	24-25	0.8136490830240533
1102	26-27	0.8523011326926948
1102	28-29	0.9433733496792627
1102	30-31	0.9692035313304856
1102	32-33	1.0265900949025237
1102	34-35	0.991253203556191
1102	36-37	1.0362940921142325
1102	38-39	1.0607936517901138
1102	40-41	1.05881814196173
1102	42-43	1.0842835079783626
1102	44-45	1.135235893625314
1102	46-47	1.1695862383058468
1102	48-49	1.1098216455248604
1102	50-51	1.0364879402227558
1102	52-53	0.9174635678975847
1102	54-55	0.7561487704237599
1102	56-57	0.6185055584530197
1102	58-59	0.6942225201497969
1102	60-61	0.7481357237177022
1102	62-63	0.7364275887450944
1102	64-65	0.710527107521937
1102	66-67	0.6958185943059512
1102	68-69	0.6646736290193154
1102	70-71	0.6641532927088782
1102	72-73	0.6805234294693498
1102	74-75	0.5986181207386672
1102	76-77	0.7208495867870788
1102	78-79	0.7249255197203333
1102	80-81	0.7419056776048443
1102	82-83	0.8038801374043096
1102	84-85	0.8369396092647641
1102	86-87	0.802617801812282
1102	88-89	0.8504862751412148
1102	90-91	0.793026443657233
1102	92-93	0.807721973888917
1102	94-95	0.9359050416063042
1102	96-97	0.9627255544053135
1102	98-99	1.0090523638712554
1102	100-101	1.123572688203307
1103	1	0.39460494246107203
1103	2	0.48072371822825133
1103	3	0.35483375195842015
1103	4	0.45164789746347367
1103	5	0.466892352986072
1103	6	0.5002579707526706
1103	7	0.3772114390096988
1103	8	0.415811077368744
1103	9	0.538698081974573
1103	10-11	0.5247960554578697
1103	12-13	0.5915186139769162
1103	14-15	0.6458247721175709
1103	16-17	0.7711322469606827
1103	18-19	0.6895095563815872
1103	20-21	0.7124197955728491
1103	22-23	0.8475589347510635
1103	24-25	0.7165381910414581
1103	26-27	0.7768141585072144
1103	28-29	0.823398783312058
1103	30-31	0.89690732599189
1103	32-33	0.9466744856285558
1103	34-35	0.9045646436519164
1103	36-37	0.9792877268790008
1103	38-39	0.7926051368135347
1103	40-41	0.9861136171719878
1103	42-43	0.8495524689981124
1103	44-45	0.8026448574333074
1103	46-47	1.1076092274892488
1103	48-49	1.1825448913717551
1103	50-51	1.1301476717646466
1103	52-53	1.0815323165656352
1103	54-55	0.967752216201216
1103	56-57	0.9554863757819199
1103	58-59	0.968297091367468
1103	60-61	0.9975127291958508
1103	62-63	0.8373124781358356
1103	64-65	0.729584414101577
1103	66-67	0.7105717348882443
1103	68-69	0.936491705238268
1103	70-71	0.8836684353791533
1103	72-73	0.9817058237712253
1103	74-75	0.9489024510285802
1103	76-77	0.9848551500513629
1103	78-79	1.0047357320245602
1103	80-81	1.0648692626921736
1103	82-83	1.050059570685434
1103	84-85	1.084777479954134
1103	86-87	1.1046465499273452
1103	88-89	1.1168969292785
1103	90-91	1.0740743326947033
1103	92-93	1.0647674375415406
1103	94-95	1.0383893697149773
1103	96-97	1.0219035788528608
1103	98-99	1.074502754983822
1103	100-101	1.1700358552967813
1104	1	0.31734422741108403
1104	2	0.3474536443290859
1104	3	0.3258147131698017
1104	4	0.3118992195170165
1104	5	0.3939732862254246
1104	6	0.3482509032121257
1104	7	0.35182097361713005
1104	8	0.26440315647909074
1104	9	0.4380890258071446
1104	10-11	0.4549838802185846
1104	12-13	0.5036547162420746
1104	14-15	0.5644033647559681
1104	16-17	0.574209733253845
1104	18-19	0.6382314858939679
1104	20-21	0.6043669548769159
1104	22-23	0.6074735468994703
1104	24-25	0.6370877063486873
1104	26-27	0.6436289990112627
1104	28-29	0.7258530270034242
1104	30-31	0.811994473203363
1104	32-33	0.7828780761067478
1104	34-35	0.8177546885149312
1104	36-37	0.7969678529040038
1104	38-39	0.8629767336622649
1104	40-41	0.8029671134880445
1104	42-43	0.9561535636319363
1104	44-45	0.9026285656284685
1104	46-47	1.0458298252768117
1104	48-49	0.8731496439615114
1104	50-51	0.9679324172125945
1104	52-53	0.8455288138039592
1104	54-55	0.8479067010449448
1104	56-57	0.7866748074036245
1104	58-59	0.8236017854606388
1104	60-61	0.8319114820904723
1104	62-63	0.6753627299918463
1104	64-65	0.5534642936687142
1104	66-67	0.7259662567350702
1104	68-69	0.7255819590576493
1104	70-71	0.6712973522143564
1104	72-73	0.6937916278187295
1104	74-75	0.7528723515858893
1104	76-77	0.7716034415626005
1104	78-79	0.6887066248570619
1104	80-81	0.8023498619915976
1104	82-83	0.7724760598940428
1104	84-85	0.7983184145389579
1104	86-87	0.819080626722112
1104	88-89	0.8172084607679011
1104	90-91	0.7768986290443465
1104	92-93	0.6047457048619123
1104	94-95	0.5186570661999284
1104	96-97	0.7078859040644723
1104	98-99	0.9598623263572392
1104	100-101	0.909793062498931
1105	1	0.2769157190392839
1105	2	0.22196031005569594
1105	3	0.2485430089992704
1105	4	0.2686231830816652
1105	5	0.24041013447257598
1105	6	0.1773151636244421
1105	7	0.1990890845442621
1105	8	0.18475999170514257
1105	9	0.18657343889065459
1105	10-11	0.20695819047465847
1105	12-13	0.18003359929235074
1105	14-15	0.08654972395859062
1105	16-17	0.07294531321377207
1105	18-19	0.0094393490033724
1105	20-21	0.1390721490290261
1105	22-23	0.06284765221296595
1105	24-25	-0.006345328483874368
1105	26-27	-0.3219547542462351
1105	28-29	-0.16611862242626785
1105	30-31	-0.3226726730232343
1105	32-33	-0.2280416757851924
1105	34-35	-0.14937399205387436
1105	36-37	-0.08160562255689996
1105	38-39	-0.0414334150802631
1105	40-41	0.0038435845686848324
1105	42-43	0.17326586869520355
1105	44-45	0.088083948928805
1105	46-47	0.12105880222909349
1105	48-49	0.12220484413995081
1105	50-51	0.18620826083443376
1105	52-53	0.055669278079243156
1105	54-55	0.004141729576435438
1105	56-57	0.10850092611032025
1105	58-59	0.14500852114944252
1105	60-61	0.06996009153947824
1105	62-63	0.11858620109988749
1105	64-65	0.03435889449400875
1105	66-67	0.007118516431145139
1105	68-69	0.09409080597794883
1105	70-71	0.06258836087310371
1105	72-73	0.018302469958641154
1105	74-75	0.07857067935801254
1105	76-77	0.05459186791371806
1105	78-79	0.10442390328642048
1105	80-81	0.10158259884361698
1105	82-83	0.11135755638587241
1105	84-85	0.05854909461656632
1105	86-87	0.13672332899830053
1105	88-89	0.1168621904486784
1105	90-91	0.1565192864794689
1105	92-93	0.22932941868359435
1105	94-95	0.2415430216314185
1105	96-97	0.28014671718230844
1105	98-99	0.25348293507966346
1105	100-101	0.21726075504986397
1106	1	-0.09989233516924045
1106	2	-0.0583672548692995
1106	3	-0.10067040591685839
1106	4	-0.13175324576001657
1106	5	-0.19486989757361783
1106	6	-0.13963059453273274
1106	7	-0.21245456057111767
1106	8	-0.2088457606041274
1106	9	-0.1519244630338079
1106	10-11	-0.3416676386836528
1106	12-13	-0.4009058732341657
1106	14-15	-0.34692401554686825
1106	16-17	-0.34066586353299755
1106	18-19	-0.33697199794300303
1106	20-21	-0.14894223464279577
1106	22-23	-0.13492934293452663
1106	24-25	-0.27838889843476267
1106	26-27	-0.2960025682255818
1106	28-29	-0.31994677900509316
1106	30-31	-0.29682517088324545
1106	32-33	-0.2391709599780043
1106	34-35	-0.2551413317292841
1106	36-37	-0.3410769649614167
1106	38-39	-0.364455473626478
1106	40-41	-0.38198732391014545
1106	42-43	-0.43332159605311205
1106	44-45	-0.4527355179550412
1106	46-47	-0.49292560570327026
1106	48-49	-0.6914155099381176
1106	50-51	-0.8437947199241194
1106	52-53	-0.8936817443311043
1106	54-55	-0.7424698502799068
1106	56-57	-0.8488140258063943
1106	58-59	-0.7606086777857755
1106	60-61	-0.8910508852084398
1106	62-63	-0.7478720340298395
1106	64-65	-0.672683698585022
1106	66-67	-0.8893370646190348
1106	68-69	-1.029941563630647
1106	70-71	-0.90878868144544
1106	72-73	-0.9388341495356443
1106	74-75	-1.0101993106986882
1106	76-77	-1.0262129140739376
1106	78-79	-0.9153207235185405
1106	80-81	-0.9124916149977658
1106	82-83	-1.048022556615411
1106	84-85	-1.0769900433613202
1106	86-87	-0.9810820863901348
1106	88-89	-1.0586922401383134
1106	90-91	-0.9645141831428177
1106	92-93	-0.977242759119612
1106	94-95	-0.993323489928521
1106	96-97	-0.9161924113800204
1106	98-99	-0.9257228955859276
1106	100-101	-1.0929276952595437
1107	1	-0.36856035301389767
1107	2	-0.44155925614926517
1107	3	-0.36808199820687904
1107	4	-0.2420286128610556
1107	5	-0.3586998727665005
1107	6	-0.3288185871897795
1107	7	-0.29144213923562035
1107	8	-0.28695323866789124
1107	9	-0.2803021714974818
1107	10-11	-0.37180254648188793
1107	12-13	-0.42536378284943765
1107	14-15	-0.6416043915708585
1107	16-17	-0.44200876624631746
1107	18-19	-0.43234200416193147
1107	20-21	-0.3325101632267504
1107	22-23	-0.375937675493347
1107	24-25	-0.4286953383671701
1107	26-27	-0.2879977080080991
1107	28-29	-0.39084553484431694
1107	30-31	-0.36125035391136606
1107	32-33	-0.34861204978201243
1107	34-35	-0.3649030793990562
1107	36-37	-0.5236381765367355
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2108	16-17	-1.3173517079068162
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2108	22-23	-1.2635180515395135
2108	24-25	-1.304633427465653
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2108	28-29	-1.743046934342928
2108	30-31	-1.9691771892551557
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2208	10-11	-0.9770583232971859
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2208	32-33	-1.436702564360381
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2308	56-57	-2.244995489231531
2308	58-59	-2.109465513774609
2308	60-61	-2.0879182433283745
2308	62-63	-2.1238890954202603
2308	64-65	-2.0874803085783533
2308	66-67	-2.3057862137766563
2308	68-69	-2.1213643861170617
2308	70-71	-2.051923043792492
2308	72-73	-2.051960560986714
2308	74-75	-1.8066477434590489
2308	76-77	-1.7238068307847776
2308	78-79	-1.8336203026629931
2308	80-81	-2.4554347586677068
2308	82-83	-2.2818737839189787
2308	84-85	-2.017994172482087
2308	86-87	-1.9196152017605286
2308	88-89	-1.8161098748692481
2308	90-91	-2.0027923928623608
2308	92-93	-2.0577110138125683
2308	94-95	-2.185836646768294
2308	96-97	-2.094433858413396
2308	98-99	-2.042401412502553
2308	100-101	-2.1952216141232874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92377.0
3	14169.0
4	12262.0
5	11333.0
6	13032.0
7	15439.0
8	18914.0
9	21530.0
10	24573.0
11	29468.0
12	29180.0
13	31878.0
14	35526.0
15	41313.0
16	51976.0
17	70477.0
18	106173.0
19	170273.0
20	240907.0
21	172870.0
22	95246.0
23	81413.0
24	84012.0
25	88978.0
26	95635.0
27	105536.0
28	120451.0
29	140782.0
30	164688.0
31	196129.0
32	240968.0
33	301210.0
34	396254.0
35	576039.0
36	923764.0
37	1192403.0
38	1043220.0
39	201585.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.047770091689506	46.74535503023069	24.07791083346646	18.12896404461334
2	16.966076817283785	48.28994284144868	18.391569544974317	16.352410796293213
3	43.711995411745725	20.335745175246668	19.22674225804542	16.725517154962187
4	16.911154222790874	18.892996179272604	20.16092472253716	44.03492487539937
5	17.062411798135344	45.815784963129445	21.154467703040588	15.967335535694621
6	43.9607414757481	19.90730450024903	19.056244425777138	17.075709598225735
7	17.818051499479257	19.434537422069674	46.85066540947644	15.896745668974626
8	43.51655670690213	20.187233819574057	19.836787487960045	16.459421985563775
9	43.082264518957345	19.80791472633999	20.496841305146155	16.612979449556516
10-11	16.64925314937819	33.03424557272269	33.3653747567503	16.951126521148822
12-13	16.5405526692668	20.141654055266926	33.251204946928	30.06658832853828
14-15	16.709898479764938	46.49635453732008	19.860588521283063	16.933158461631916
16-17	29.985276041811737	19.95825775779406	19.987014842268323	30.06945135812588
18-19	16.853035375053725	33.122592713577575	19.98134083707829	30.04303107429041
20-21	30.120402184953864	33.11547866861191	20.016172944190792	16.747946202243433
22-23	30.017265760685923	19.86384986268677	33.33920969932701	16.779674677300292
24-25	16.79908766812432	32.89902747247709	20.375926677551202	29.92595818184739
26-27	16.991724018204028	25.078975068085164	40.93685297030989	16.992447943400908
28-29	27.61512726420314	19.76619996518268	35.70985106115266	16.90882170946152
30-31	25.777472172696992	20.711734713400894	36.03534128327888	17.475451830623236
32-33	20.012660939368068	22.884537596833194	37.526316917983735	19.576484545815
34-35	19.61051296063183	22.142229046933725	38.357866305059915	19.889391687374527
36-37	19.327334980370818	22.219203986424557	38.33219597935141	20.121265053853215
38-39	19.983393704610368	22.222147913926356	37.886160487441586	19.908297894021693
40-41	19.894989250925207	22.20909637653346	37.848705453701555	20.04720891883978
42-43	20.443405520972014	22.25136907392482	37.69497108794408	19.610254317159082
44-45	20.195149206216133	22.30018789485031	37.63003046870847	19.874632430225088
46-47	20.261552275982726	22.17182347227669	37.599018720481226	19.96760553125936
48-49	20.15222940835879	22.331190178377767	37.57595233699127	19.940628076272176
50-51	20.284994300486307	22.508318297690085	36.79172088495835	20.414966516865256
52-53	20.925465995438657	23.2905561930255	35.501473620962905	20.28250419057294
54-55	21.578390155954267	24.055667033375574	33.8805269976344	20.48541581303576
56-57	21.88833353651103	24.984119695457107	32.439903695151166	20.687643072880693
58-59	21.862033791116804	25.719533026487746	31.578658914836232	20.83977426755922
60-61	21.94691148663725	26.014404046400873	31.19827887443567	20.840405592526203
62-63	21.806933848539728	25.74087204599697	31.403817100165355	21.04837700529795
64-65	21.507282612349314	25.434260397418885	31.74228933060347	21.316167659628334
66-67	21.38788804636114	25.034374013984024	32.35439095887674	21.223346980778093
68-69	21.489631705922548	24.80134575813771	32.60798008010649	21.101042455833248
70-71	21.29854224845392	24.834423600576738	32.94656963417052	20.920464516798823
72-73	21.148110155339666	24.813457534385755	32.778292452258476	21.260139858016107
74-75	21.40393518103086	25.005321879121695	32.15311632951309	21.437626610334355
76-77	21.578269898506026	25.25843609728875	31.71088541553769	21.45240858866754
78-79	21.62615659032529	25.458888851682687	31.375356474748244	21.53959808324378
80-81	21.70723663840515	25.755070501459304	30.979317450245947	21.558375409889596
82-83	21.990339423316772	25.943056130929516	30.689234424628882	21.377370021124833
84-85	21.983162337144638	26.35096652795768	30.017425769638155	21.648445365259526
86-87	22.01517585343036	26.427745027936243	29.93116700500231	21.62591211363108
88-89	22.030920655785774	26.33531288867298	29.891851630052933	21.741914825488312
90-91	22.00073380511779	26.364975889013877	29.76902396671024	21.865266339158097
92-93	22.018968168947413	26.48096044116465	29.73424044059022	21.765830949297715
94-95	21.971183560403983	26.36602652153147	29.426866099126414	22.23592381893813
96-97	21.82279471832914	26.146234533977346	29.75872428095212	22.27224646674139
98-99	22.255057424707417	26.300638193448812	29.41928821488854	22.025016166955233
100-101	22.50885568542805	26.18966885252103	29.109052535196668	22.192422926854256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6468.0
1	3855.5
2	898.5
3	494.5
4	431.0
5	418.5
6	489.5
7	605.0
8	601.5
9	681.0
10	1121.5
11	5794.0
12	109250.0
13	122854.0
14	33975.5
15	31658.0
16	34058.5
17	37127.5
18	40076.0
19	42120.0
20	44356.5
21	47238.0
22	50332.0
23	53527.5
24	57478.5
25	62238.0
26	68040.5
27	74788.5
28	81668.5
29	89036.0
30	96728.5
31	104607.0
32	112683.0
33	121214.0
34	133302.0
35	142045.0
36	148620.0
37	157092.5
38	163336.5
39	169612.0
40	177405.0
41	185373.5
42	189893.0
43	195186.5
44	205627.0
45	213529.5
46	216384.0
47	220953.0
48	225000.5
49	227227.0
50	229182.5
51	231155.0
52	234811.0
53	235428.5
54	230435.5
55	223382.0
56	212681.5
57	200781.0
58	195062.0
59	171454.0
60	135029.5
61	113219.5
62	91955.0
63	68276.0
64	51077.0
65	38757.0
66	26393.0
67	16675.0
68	10784.5
69	6675.0
70	4414.5
71	3047.0
72	2402.5
73	1997.0
74	1734.0
75	1570.5
76	1347.5
77	1188.5
78	1082.5
79	976.5
80	851.0
81	725.0
82	668.5
83	571.5
84	473.5
85	401.0
86	330.5
87	254.0
88	185.0
89	138.0
90	112.0
91	87.0
92	51.5
93	44.5
94	39.0
95	25.5
96	19.5
97	12.5
98	5.5
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050315467917994475
2	0.09111663798358659
3	0.08154718478131524
4	0.05356963356026543
5	0.08384992063834597
6	0.0015305609588647264
7	0.0012685730469869805
8	0.004798515438602926
9	0.009266098777991857
10-11	0.009548769946070478
12-13	0.01651213286703162
14-15	0.011465418354018198
16-17	0.0016132939836682252
18-19	5.791311736244911E-4
20-21	1.034162810043734E-4
22-23	3.92981867816619E-4
24-25	7.928581543668627E-4
26-27	0.001551244215065601
28-29	0.0014547223527948527
30-31	0.004515844270524305
32-33	2.275158182096215E-4
34-35	0.0022889470195634645
36-37	4.136651240174936E-4
38-39	0.002399257719301463
40-41	0.0030818051739303276
42-43	0.0010755293224454835
44-45	6.687586171616147E-4
46-47	0.0010893181599127334
48-49	0.0022337916696944654
50-51	0.00425385635864656
52-53	0.12685730469869805
54-55	0.13644744115717028
56-57	0.12401680418044458
58-59	0.1176808333642433
60-61	0.10591895500467925
62-63	0.11067610393088043
64-65	0.11270306303856614
66-67	0.12082468830677626
68-69	0.13284166015948445
70-71	0.12077642737564089
72-73	0.11282026815703776
74-75	0.11804623755712544
76-77	0.1289256303187855
78-79	0.14230769708075144
80-81	0.13225563456712636
82-83	0.11254449140769276
84-85	0.11941822688511679
86-87	0.11991462503393778
88-89	0.11643983799219082
90-91	0.12651947818075043
92-93	0.14231459149948505
94-95	0.15160137353367778
96-97	0.15288373541813202
98-99	0.13577178812127505
100-101	0.12625059585013906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7252243.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6797779539735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.91383040525575	52.09655498269075
2	4.868003402473289	5.518347038627471
3	1.1800231007522994	2.006503419935989
4	0.5852328409280281	1.326834699006949
5	0.3100609342935091	0.8787092453978832
6	0.2318964161351928	0.788630242491898
7	0.17175664828286016	0.6814590080753867
8	0.12183537690639802	0.5524481687994649
9	0.10565833439910075	0.5389821839454939
>10	0.4827840649553115	4.40715644916411
>50	0.014535316736498535	0.5437808833163513
>100	0.00948092435421235	1.2744610144782063
>500	0.002107960846917215	0.8687216754283587
>1k	0.0024511172637592675	2.633450639043194
>5k	2.4511172637592675E-4	1.0239051251753242
>10k+	9.80446905503707E-5	24.86005522442303
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	876704	12.088728962887757	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	18001	0.24821286324796343	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9251	0.12756053540952778	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	7713	0.10635330338489761	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	7642	0.10537429592472289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3788837467249788E-5	0.0
2	1.3788837467249788E-5	0.0	0.0	1.3788837467249788E-5	0.0
3	5.515534986899915E-5	0.0	0.0	1.3788837467249788E-5	0.0
4	6.894418733624894E-5	0.0	0.0	2.7577674934499576E-5	2.7577674934499576E-5
5	8.273302480349873E-5	0.0	0.0	2.7577674934499576E-5	2.7577674934499576E-5
6	8.273302480349873E-5	0.0	0.0	2.7577674934499576E-5	2.7577674934499576E-5
7	9.652186227074852E-5	0.0	0.0	4.1366512401749364E-5	2.7577674934499576E-5
8	9.652186227074852E-5	0.0	0.0	4.1366512401749364E-5	2.7577674934499576E-5
9	9.652186227074852E-5	0.0	0.0	4.1366512401749364E-5	2.7577674934499576E-5
10-11	9.652186227074852E-5	0.0	0.0	5.515534986899915E-5	2.7577674934499576E-5
12-13	1.103106997379983E-4	0.0	0.0	6.894418733624894E-5	2.7577674934499576E-5
14-15	1.2409953720524808E-4	0.0	0.0	7.583860606987384E-5	4.826093113537426E-5
16-17	1.5167721213974765E-4	0.0	0.0	9.652186227074852E-5	5.515534986899915E-5
18-19	1.5857163087337257E-4	0.0	0.0	1.2409953720524808E-4	5.515534986899915E-5
20-21	1.8614930580787212E-4	0.0	0.0	1.3788837467249788E-4	6.894418733624894E-5
22-23	2.068325620087468E-4	0.0	0.0	1.5167721213974765E-4	7.583860606987384E-5
24-25	2.068325620087468E-4	0.0	0.0	1.5167721213974765E-4	1.103106997379983E-4
26-27	2.068325620087468E-4	0.0	0.0	1.6546604960699746E-4	1.103106997379983E-4
28-29	2.068325620087468E-4	0.0	0.0	1.7925488707424723E-4	1.103106997379983E-4
30-31	2.4130465567687127E-4	0.0	0.0	1.7925488707424723E-4	1.103106997379983E-4
32-33	2.4819907441049616E-4	6.894418733624894E-6	0.0	1.7925488707424723E-4	1.3099395593887297E-4
34-35	2.7577674934499576E-4	1.3788837467249788E-5	0.0	1.7925488707424723E-4	1.3788837467249788E-4
36-37	2.8956558681224553E-4	1.3788837467249788E-5	0.0	1.7925488707424723E-4	1.5857163087337257E-4
38-39	3.1714326174674514E-4	1.3788837467249788E-5	0.0	1.8614930580787212E-4	1.6546604960699746E-4
40-41	3.1714326174674514E-4	2.0683256200874682E-5	0.0	1.9304372454149703E-4	1.7925488707424723E-4
42-43	3.2403768048037E-4	2.7577674934499576E-5	0.0	1.9304372454149703E-4	1.8614930580787212E-4
44-45	3.447209366812447E-4	2.7577674934499576E-5	0.0	1.9993814327512192E-4	2.068325620087468E-4
46-47	3.7229861161574423E-4	2.7577674934499576E-5	0.0	2.068325620087468E-4	2.068325620087468E-4
48-49	4.067707052838687E-4	2.7577674934499576E-5	0.0	2.068325620087468E-4	2.068325620087468E-4
50-51	4.412427989519932E-4	2.7577674934499576E-5	0.0	2.068325620087468E-4	2.068325620087468E-4
52-53	4.8260931135374254E-4	2.7577674934499576E-5	0.0	2.1372698074237172E-4	2.206213994759966E-4
54-55	5.239758237554919E-4	2.7577674934499576E-5	0.0	2.3441023694324638E-4	2.3441023694324638E-4
56-57	5.998144298253658E-4	2.7577674934499576E-5	0.0	2.3441023694324638E-4	2.3441023694324638E-4
58-59	6.067088485589906E-4	2.7577674934499576E-5	0.0	2.3441023694324638E-4	2.3441023694324638E-4
60-61	6.411809422271152E-4	2.7577674934499576E-5	0.0	2.3441023694324638E-4	2.4130465567687127E-4
62-63	6.963362920961143E-4	2.7577674934499576E-5	0.0	2.3441023694324638E-4	2.4819907441049616E-4
64-65	7.239139670306139E-4	2.7577674934499576E-5	0.0	2.4130465567687127E-4	2.4819907441049616E-4
66-67	7.79069316899613E-4	2.7577674934499576E-5	0.0	2.4819907441049616E-4	2.550934931441211E-4
68-69	8.962744353712362E-4	2.7577674934499576E-5	0.0	2.6198791187774593E-4	2.8267116807862065E-4
70-71	9.583242039738602E-4	2.7577674934499576E-5	0.0	2.8956558681224553E-4	3.033544242794953E-4
72-73	0.0010479516475109837	2.7577674934499576E-5	0.0	3.1714326174674514E-4	3.447209366812447E-4
74-75	0.0011237902535808576	2.7577674934499576E-5	0.0	3.447209366812447E-4	3.6540419288211935E-4
76-77	0.0012823618844542304	2.7577674934499576E-5	0.0	3.447209366812447E-4	3.7229861161574423E-4
78-79	0.0013719893279913538	2.7577674934499576E-5	0.0	3.447209366812447E-4	3.7229861161574423E-4
80-81	0.0015719274712664758	2.7577674934499576E-5	0.0	3.5850977414849446E-4	3.7229861161574423E-4
82-83	0.0017994432894760974	4.1366512401749364E-5	0.0	3.5850977414849446E-4	3.9987628655024384E-4
84-85	0.002075220038821093	4.826093113537426E-5	0.0	3.5850977414849446E-4	4.2745396148474344E-4
86-87	0.002488885162838587	5.515534986899915E-5	0.0	3.5850977414849446E-4	4.55031636419243E-4
88-89	0.0031576437800002015	5.515534986899915E-5	0.0	3.5850977414849446E-4	4.619260551528679E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	194170	0.0	91.81723	1
AGTGGTA	193825	0.0	91.612465	5
GGTATCA	194640	0.0	91.409225	8
GTATCAA	194725	0.0	91.388275	9
TGGTATC	194665	0.0	91.3237	7
GTGGTAT	195335	0.0	91.117935	6
AGCAGTG	197025	0.0	90.516396	2
GCAGTGG	197020	0.0	90.42822	3
CAGTGGT	196925	0.0	90.231155	4
TCAACGC	193875	0.0	46.324806	12-13
CAACGCA	193855	0.0	46.27816	12-13
ATCAACG	194050	0.0	46.20506	10-11
AACGCAG	194775	0.0	46.13698	14-15
ACGCAGA	195015	0.0	46.055855	14-15
CGCAGAG	195860	0.0	45.870323	16-17
TATCAAC	195380	0.0	45.852867	10-11
AGAGTAC	191675	0.0	45.68976	18-19
CAGAGTA	194860	0.0	45.594494	18-19
GCAGAGT	197710	0.0	45.275463	16-17
GAGTACT	112960	0.0	45.25248	20-21
>>END_MODULE