FastQCFastQC Report
Sat 18 Jun 2016
SRR578572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7252243
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA692140.9543805964582267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91240.12580935305118707No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88280.12172785716088114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4050.023.4179943
CAACGCA58450.020.19648412-13
ATCAACG61500.019.27195510-11
CGTACGC2450.018.38619420-21
GGTATCA72250.018.3105818
AAGCAGT77300.018.2227761
GTATCAA75600.017.8113339
AGTGGTA76550.017.653815
TCGCGTA2750.017.23964152-53
TCAACGC57000.017.21695910-11
CTCGCGT2750.016.37765952-53
CGCGTAG2750.016.37765954-55
TGGTATC82400.016.1701817
ACGCAGA73800.015.99572514-15
GTGGTAT85750.015.7596466
AACGCAG63650.015.52989712-13
ACTCGCG3100.015.2932350-51
ACGTACG3100.014.53102320-21
AGAGTAC83700.013.96316318-19
TATCAAC76400.013.5909759
Produced by FastQC (version 0.11.5)