FastQCFastQC Report
Sat 18 Jun 2016
SRR578571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8510805
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110368712.96806823796339No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT236350.277705810437438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA109760.12896547388878019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99530.11694545933081536No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG89950.1056891798131904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2423550.091.973421
AGTGGTA2417200.091.825325
GGTATCA2425000.091.6626748
GTATCAA2429150.091.513379
TGGTATC2426950.091.5013667
GTGGTAT2438700.091.0973746
GCAGTGG2446300.090.861323
AGCAGTG2450650.090.859942
CAGTGGT2443150.090.6401144
TCAACGC2423050.046.3684512-13
AACGCAG2428550.046.3038914-15
CAACGCA2423250.046.24612-13
ATCAACG2423600.046.23980710-11
CGCAGAG2441450.046.0931916-17
ACGCAGA2432950.046.08931714-15
TATCAAC2437350.046.01891710-11
CAGAGTA2425850.045.84394518-19
AGAGTAC2382000.045.735718-19
GAGTACT1384400.045.6770220-21
GCAGAGT2456050.045.41882316-17
Produced by FastQC (version 0.11.5)