FastQCFastQC Report
Sat 18 Jun 2016
SRR578571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8510805
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1362671.6011058883384122No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165800.19481118413593074No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101900.11973015478559314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4450.026.6850173
ACTCGCG2700.022.87233250-51
CTCGCGT2650.022.40646452-53
CGCGTAG2850.021.66661554-55
AGTGGTA75250.019.6306085
CAACGCA61500.019.30787812-13
ATCAACG61400.019.26196510-11
GGTATCA79750.018.4629298
CGTACGC3400.018.16076320-21
GTATCAA82100.017.87669
AAGCAGT86150.017.6156861
TCGCGTA3500.016.96489352-53
TGGTATC88250.016.7922747
CGCTAGT3450.016.52106990-91
CGTAGCG3700.016.04688356-57
TCAACGC61850.015.550879510-11
ACGCAGA76250.015.47947314-15
AACGCAG63900.015.20064612-13
ACGTACG4100.014.480906520-21
GTGGTAT104400.014.4677626
Produced by FastQC (version 0.11.5)