FastQCFastQC Report
Sat 18 Jun 2016
SRR578570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574470
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111996613.06163529640899No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT242390.2826880261987038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99580.1161354579350094No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG95340.11119054588796741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2436050.091.8073041
AGTGGTA2426000.091.748265
GGTATCA2440450.091.522548
TGGTATC2437450.091.386197
GTATCAA2448350.091.3411569
GTGGTAT2451000.090.986216
AGCAGTG2463250.090.7184452
GCAGTGG2460450.090.669973
CAGTGGT2458700.090.529264
GTATCGA6400.047.5152179
TCAACGC2439750.046.32058712-13
AACGCAG2445350.046.2551814-15
ATCAACG2436450.046.23925810-11
CAACGCA2438400.046.2254212-13
ACGCAGA2450300.046.06672314-15
CGCAGAG2455750.046.04332716-17
TATCAAC2455350.045.9520310-11
CAGAGTA2439350.045.75216318-19
AGAGTAC2391950.045.73433318-19
GAGTACT1400600.045.51958520-21
Produced by FastQC (version 0.11.5)