##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8574470 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.550552978784694 31.0 31.0 33.0 27.0 34.0 2 30.847012118533275 31.0 31.0 34.0 27.0 34.0 3 30.9464167464578 31.0 31.0 34.0 27.0 34.0 4 34.38261595177311 37.0 35.0 37.0 30.0 37.0 5 34.42612616289986 37.0 35.0 37.0 30.0 37.0 6 34.421859310254746 37.0 35.0 37.0 30.0 37.0 7 34.31861689410541 37.0 35.0 37.0 30.0 37.0 8 34.281496232420196 37.0 35.0 37.0 30.0 37.0 9 35.70701046245424 39.0 35.0 39.0 30.0 39.0 10-11 35.67235117739055 39.0 35.0 39.0 30.0 39.0 12-13 35.74029117834688 39.0 35.0 39.0 30.0 39.0 14-15 36.765666391042245 40.0 36.0 41.0 30.0 41.0 16-17 36.730135098729136 40.0 36.0 41.0 30.0 41.0 18-19 36.712553428958294 40.0 36.0 41.0 30.0 41.0 20-21 36.59291005741463 39.5 36.0 41.0 30.0 41.0 22-23 36.273231814911014 39.0 36.0 40.5 28.5 41.0 24-25 36.166991895709 39.0 36.0 40.0 29.0 41.0 26-27 36.11087093429681 39.0 36.0 40.0 28.0 41.0 28-29 35.7997292544029 39.0 35.0 41.0 27.0 41.0 30-31 35.52630809834311 38.5 34.5 40.5 25.5 41.0 32-33 35.15027132872352 38.0 34.0 40.0 25.0 41.0 34-35 34.936271221428264 38.0 34.0 40.0 25.0 41.0 36-37 34.95040072447627 38.0 34.0 40.0 25.0 41.0 38-39 34.24467226545781 37.0 33.5 40.0 24.5 41.0 40-41 34.21792081609709 37.0 33.0 40.0 24.0 41.0 42-43 34.035458168259964 37.0 33.0 40.0 23.5 41.0 44-45 33.87259527411024 37.0 33.0 40.0 23.0 41.0 46-47 33.664962265889315 37.0 33.0 40.0 22.5 41.0 48-49 33.297027688008704 36.5 33.0 40.0 20.5 41.0 50-51 32.00029418727921 35.5 31.0 39.0 14.0 40.5 52-53 31.57619701275997 35.5 31.0 39.0 6.5 40.0 54-55 31.22549043847608 36.0 30.0 40.0 2.0 41.0 56-57 30.713257262548005 36.0 29.0 40.0 2.0 41.0 58-59 30.387480742249956 36.0 28.0 40.0 2.0 41.0 60-61 30.07762952112492 35.5 28.0 39.0 2.0 41.0 62-63 29.73450300718295 35.0 28.0 39.0 2.0 41.0 64-65 29.407316253949226 35.0 27.0 39.0 2.0 40.5 66-67 29.104555908411832 35.0 26.5 38.5 2.0 40.0 68-69 28.770326562458088 35.0 26.0 38.0 2.0 40.0 70-71 28.38474937809567 34.5 26.0 37.0 2.0 40.0 72-73 28.005306858616336 34.0 26.0 37.0 2.0 39.0 74-75 27.60705069817726 34.0 25.0 36.0 2.0 39.0 76-77 27.196366947461478 34.0 24.0 36.0 2.0 38.5 78-79 26.80700340662455 34.0 23.5 35.0 2.0 37.5 80-81 26.418210629928147 33.5 22.0 35.0 2.0 37.0 82-83 26.05623373806194 33.0 20.0 35.0 2.0 36.5 84-85 25.654648392262146 33.0 19.5 35.0 2.0 36.0 86-87 25.295767551813697 33.0 18.0 35.0 2.0 36.0 88-89 24.90159415100875 32.0 13.0 35.0 2.0 35.0 90-91 24.522096351144732 32.0 7.0 35.0 2.0 35.0 92-93 24.148846750877897 32.0 2.0 35.0 2.0 35.0 94-95 23.690729747727847 31.5 2.0 35.0 2.0 35.0 96-97 23.19767559977468 31.0 2.0 34.5 2.0 35.0 98-99 22.57457084811073 31.0 2.0 34.0 2.0 35.0 100-101 21.001568260195675 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5417153700143373 1101 2 0.6302675384460166 1101 3 0.6429496055692141 1101 4 0.7293886656227073 1101 5 0.7052813810986649 1101 6 0.5973026094098586 1101 7 0.6330593240971396 1101 8 0.6421130706632496 1101 9 0.6320272330352807 1101 10-11 0.7358601125780808 1101 12-13 0.710046590772194 1101 14-15 0.8370849759117007 1101 16-17 0.8123677077965681 1101 18-19 0.786932867290254 1101 20-21 0.8200954944841712 1101 22-23 0.8923219171502268 1101 24-25 1.0414900787060262 1101 26-27 0.9801901558487032 1101 28-29 0.9773076658704554 1101 30-31 1.0494711138906396 1101 32-33 1.1099070659106687 1101 34-35 1.1297229408989296 1101 36-37 1.0616845993689523 1101 38-39 1.1372267477583335 1101 40-41 1.0760892339740238 1101 42-43 1.2737726331197905 1101 44-45 1.1550987161595714 1101 46-47 1.0881381674169148 1101 48-49 1.1973385736978415 1101 50-51 1.2883907006334283 1101 52-53 1.3809031228273874 1101 54-55 1.1711652806761208 1101 56-57 1.2010939417760582 1101 58-59 1.1954309294341527 1101 60-61 1.2571928903037666 1101 62-63 1.3842024565590556 1101 64-65 1.3330533827932634 1101 66-67 1.2959719470890363 1101 68-69 1.3213135599450077 1101 70-71 1.2871401229136943 1101 72-73 1.3407080822392174 1101 74-75 1.3579961334656971 1101 76-77 1.3269981404601978 1101 78-79 1.3742164278805866 1101 80-81 1.402476738532453 1101 82-83 1.424818421641774 1101 84-85 1.5052508171205297 1101 86-87 1.5551077158421798 1101 88-89 1.5793201690446637 1101 90-91 1.7140164048736146 1101 92-93 1.8099606276356255 1101 94-95 1.8673793758226083 1101 96-97 2.167314643285028 1101 98-99 2.4161867220095985 1101 100-101 2.765587672859091 1102 1 0.48715229654967374 1102 2 0.4683709722657312 1102 3 0.527775778785287 1102 4 0.6033218995498331 1102 5 0.5304588732976185 1102 6 0.506832801778998 1102 7 0.5509608824933707 1102 8 0.6375239937133301 1102 9 0.5783970841752435 1102 10-11 0.646376511203016 1102 12-13 0.6465477368517298 1102 14-15 0.7690005999840182 1102 16-17 0.8295176436659659 1102 18-19 0.7043545084217655 1102 20-21 0.7370088071355738 1102 22-23 0.7966760704563498 1102 24-25 0.8290251749132622 1102 26-27 0.9074931072159558 1102 28-29 0.7861257224866733 1102 30-31 0.8544702556056691 1102 32-33 0.9329565070906725 1102 34-35 1.0630231797457625 1102 36-37 0.8844085154371797 1102 38-39 1.0448581333418616 1102 40-41 1.045285358038413 1102 42-43 1.1814201788213339 1102 44-45 1.0905906409160266 1102 46-47 1.2181662585458213 1102 48-49 1.3302062425845236 1102 50-51 1.1994081620587593 1102 52-53 1.1733526936282423 1102 54-55 1.1469206422662026 1102 56-57 1.0756542760936334 1102 58-59 1.1851383647313618 1102 60-61 1.1871750504399152 1102 62-63 1.1893616981637258 1102 64-65 1.1836735149294917 1102 66-67 1.1720167744916097 1102 68-69 1.2707563120541785 1102 70-71 1.1814689412337422 1102 72-73 1.2398086250832172 1102 74-75 1.3033135940978902 1102 76-77 1.1788403514249453 1102 78-79 1.1472510686019284 1102 80-81 1.2274449423317968 1102 82-83 1.367174445046043 1102 84-85 1.4862328934110174 1102 86-87 1.5457192537982074 1102 88-89 1.6811252868024944 1102 90-91 1.6645410492660382 1102 92-93 1.5389687617977472 1102 94-95 1.610392907832388 1102 96-97 1.8721439692112405 1102 98-99 2.012066869170063 1102 100-101 2.337109168507048 1103 1 0.41048188093481386 1103 2 0.3922650931186382 1103 3 0.2369777300668794 1103 4 0.4121439184057536 1103 5 0.30347449641408986 1103 6 0.2408522781157103 1103 7 0.48024974482587623 1103 8 0.3872172322902898 1103 9 0.6188758368822107 1103 10-11 0.5319548569747141 1103 12-13 0.4031653549699996 1103 14-15 0.6765070287604757 1103 16-17 0.6260779071282059 1103 18-19 0.6362265039433268 1103 20-21 0.8001041126579409 1103 22-23 0.79635947610128 1103 24-25 0.6988534057959939 1103 26-27 0.9106117100673927 1103 28-29 0.8416834410434788 1103 30-31 0.7078744729049617 1103 32-33 0.9578087354017555 1103 34-35 0.8526521518148868 1103 36-37 0.9291850617137385 1103 38-39 0.6995223921973022 1103 40-41 0.7775803753475259 1103 42-43 0.6326338876565671 1103 44-45 0.7375140577351118 1103 46-47 0.901396988364688 1103 48-49 0.9514047275175841 1103 50-51 0.9561314944819443 1103 52-53 0.9072930402329256 1103 54-55 0.9679723189182567 1103 56-57 0.9241034499147744 1103 58-59 0.9417562777004527 1103 60-61 0.9014770295197643 1103 62-63 0.8678491833920425 1103 64-65 1.0069094807503838 1103 66-67 0.8774115502561877 1103 68-69 1.0020602534691037 1103 70-71 1.0228791619700424 1103 72-73 1.0443262813675211 1103 74-75 1.2046990089660277 1103 76-77 1.2481321869508477 1103 78-79 1.2694702927931907 1103 80-81 1.2136975776467231 1103 82-83 1.289092928256622 1103 84-85 1.2731049760164233 1103 86-87 1.4388491885209795 1103 88-89 1.6140035807490172 1103 90-91 1.3407018940918114 1103 92-93 1.4337865254763216 1103 94-95 1.442854988988227 1103 96-97 1.6175319606641345 1103 98-99 1.6990442340896443 1103 100-101 1.9996557772220456 1104 1 0.20785848146466535 1104 2 0.2713516361432191 1104 3 0.2503990598248116 1104 4 0.32892000323162307 1104 5 0.11280454997340428 1104 6 0.14542875673041067 1104 7 0.311996643788099 1104 8 0.25690101848765323 1104 9 0.4409744822439876 1104 10-11 0.44442137896984235 1104 12-13 0.33539681199629 1104 14-15 0.4277392648900573 1104 16-17 0.577291002922216 1104 18-19 0.6515278679775705 1104 20-21 0.5388867983007373 1104 22-23 0.40756804749934616 1104 24-25 0.5435723831908774 1104 26-27 0.6071236116440559 1104 28-29 0.7833533755750324 1104 30-31 0.7209169477985782 1104 32-33 0.843625427920415 1104 34-35 0.7270741757716763 1104 36-37 0.7923591378010499 1104 38-39 0.6158733386626523 1104 40-41 0.6815531858899888 1104 42-43 0.6702594028384325 1104 44-45 1.0108583179030646 1104 46-47 0.7627252772836641 1104 48-49 0.840052550018406 1104 50-51 0.8422823792957956 1104 52-53 0.6807958297479537 1104 54-55 0.7718883554852454 1104 56-57 0.9358494577689846 1104 58-59 0.985599876777993 1104 60-61 0.8932639594864327 1104 62-63 0.8207434484837322 1104 64-65 0.953614021472152 1104 66-67 0.7424313197391932 1104 68-69 0.8734644394181608 1104 70-71 1.069421604625962 1104 72-73 0.9070734374141303 1104 74-75 1.0058276671857698 1104 76-77 1.1069553330767938 1104 78-79 1.1258896509545657 1104 80-81 1.0170773284818324 1104 82-83 1.0648125163927133 1104 84-85 1.054731322881235 1104 86-87 1.1974729927674268 1104 88-89 1.3283368186262656 1104 90-91 1.413371651674396 1104 92-93 1.276342502953689 1104 94-95 1.40060063522834 1104 96-97 1.6293733419919256 1104 98-99 1.815507690308852 1104 100-101 1.966512326341448 1105 1 -0.04496624061442844 1105 2 0.10054724231708434 1105 3 0.146965613178498 1105 4 0.13170194360002796 1105 5 0.14030319656714596 1105 6 0.1496252838024148 1105 7 0.317744265813765 1105 8 0.23725726777423262 1105 9 0.21432599189787993 1105 10-11 0.28700242230014794 1105 12-13 0.22615633674261915 1105 14-15 0.05385899564480923 1105 16-17 0.25521743842554656 1105 18-19 0.07176198425333524 1105 20-21 0.08389483952949917 1105 22-23 0.3430152280757923 1105 24-25 0.49457711574859786 1105 26-27 0.45697394422735016 1105 28-29 0.5611364752369283 1105 30-31 0.42138700189561007 1105 32-33 0.5901625817450622 1105 34-35 0.5155774270099442 1105 36-37 0.7043350214717066 1105 38-39 0.6175384980982486 1105 40-41 0.504065231199256 1105 42-43 0.7332585382836143 1105 44-45 0.7334977227206352 1105 46-47 0.5649462067594229 1105 48-49 0.6965030819976192 1105 50-51 0.7198841408476966 1105 52-53 0.5661206391745814 1105 54-55 0.7092375207644537 1105 56-57 0.6789324937639378 1105 58-59 0.7080955586821105 1105 60-61 0.6606218192745423 1105 62-63 0.7552389638187194 1105 64-65 0.8015805245679992 1105 66-67 0.6883186655323321 1105 68-69 0.7738829808954506 1105 70-71 0.7856231111358589 1105 72-73 0.6826519120474224 1105 74-75 0.5913318550101287 1105 76-77 0.5456334050039473 1105 78-79 0.732691989095084 1105 80-81 0.7422812840296622 1105 82-83 0.8535506383126545 1105 84-85 0.9126745557717726 1105 86-87 0.8763819118780667 1105 88-89 0.8694879243871831 1105 90-91 1.1517494621023694 1105 92-93 1.187856104728212 1105 94-95 1.2387097343710423 1105 96-97 1.3658634970716896 1105 98-99 1.6179723662069243 1105 100-101 2.001451894018924 1106 1 0.2560583232824918 1106 2 0.27624569571334234 1106 3 0.23133838078791058 1106 4 0.09053492858642187 1106 5 0.2968992914056017 1106 6 0.2647967582098687 1106 7 0.40979782285263866 1106 8 0.3508976023532071 1106 9 0.4435886166879115 1106 10-11 0.2244286500270789 1106 12-13 0.41245833244226304 1106 14-15 0.2749336126410782 1106 16-17 0.3586685492423669 1106 18-19 0.3656364486439969 1106 20-21 0.27692230944821716 1106 22-23 0.35837095169130606 1106 24-25 0.5691676306286055 1106 26-27 0.450108184446421 1106 28-29 0.438038038250518 1106 30-31 0.5584741591792266 1106 32-33 0.522511263791742 1106 34-35 0.48789025726210866 1106 36-37 0.6159743272477414 1106 38-39 0.5948608874462877 1106 40-41 0.47638764735748396 1106 42-43 0.7106974507694019 1106 44-45 0.6708195298323787 1106 46-47 0.6661658248751365 1106 48-49 0.8346211794497052 1106 50-51 0.7186251876367322 1106 52-53 0.6446257320475972 1106 54-55 0.7957787771383238 1106 56-57 0.7620800611746077 1106 58-59 0.868641628546623 1106 60-61 0.8647020285720544 1106 62-63 0.863807317095624 1106 64-65 0.9678370248998895 1106 66-67 0.9340512300844317 1106 68-69 0.9595385223129895 1106 70-71 0.9370453712588009 1106 72-73 0.8945887830640196 1106 74-75 0.8198064879710962 1106 76-77 0.9417762589816121 1106 78-79 0.9824489395851721 1106 80-81 1.080711874431433 1106 82-83 1.186999630618942 1106 84-85 1.233340868813773 1106 86-87 1.2837886480940703 1106 88-89 1.334722536782209 1106 90-91 1.2529115141964802 1106 92-93 1.334117467314929 1106 94-95 1.351980771286847 1106 96-97 1.5896899520073582 1106 98-99 1.7304699292622026 1106 100-101 1.8980459052506724 1107 1 0.12213010037806171 1107 2 0.18323688026517715 1107 3 0.22944780677875087 1107 4 0.17233717724117525 1107 5 0.21508271808882995 1107 6 0.2308050386830658 1107 7 0.23354875453121338 1107 8 0.2844897738026333 1107 9 0.17124071147276965 1107 10-11 0.25057910711936415 1107 12-13 0.2940422029035261 1107 14-15 0.3094318624487542 1107 16-17 0.24038791565847362 1107 18-19 0.2770392837708684 1107 20-21 0.2845145593763263 1107 22-23 0.4309887322598982 1107 24-25 0.49508913741524196 1107 26-27 0.42820182414811825 1107 28-29 0.5216396435130761 1107 30-31 0.5689626511294676 1107 32-33 0.5774865604004944 1107 34-35 0.6352494943080984 1107 36-37 0.5659251284286526 1107 38-39 0.5568276244044128 1107 40-41 0.44195853322886336 1107 42-43 0.4246138637573935 1107 44-45 0.3915119747856437 1107 46-47 0.5017830315875287 1107 48-49 0.483945593856248 1107 50-51 0.40185536080807793 1107 52-53 0.42807259642768614 1107 54-55 0.5376563996066395 1107 56-57 0.35971910619236525 1107 58-59 0.387815188658454 1107 60-61 0.3147985320294886 1107 62-63 0.4200438298507905 1107 64-65 0.4687306471971233 1107 66-67 0.49384421748706586 1107 68-69 0.5343135330321864 1107 70-71 0.5948389415217044 1107 72-73 0.6171446107483654 1107 74-75 0.6516672503122152 1107 76-77 0.7098905614966817 1107 78-79 0.7124857516643779 1107 80-81 0.676009041847184 1107 82-83 0.824859225039674 1107 84-85 0.8508474398687795 1107 86-87 0.8113483961314465 1107 88-89 0.9489067007251002 1107 90-91 0.903520108973229 1107 92-93 0.9276286499390736 1107 94-95 1.0344469347724754 1107 96-97 1.1228814728920256 1107 98-99 1.2718091260548725 1107 100-101 1.5369981644263717 1108 1 0.1798930675032473 1108 2 0.12120281052112958 1108 3 -0.0453328462703837 1108 4 0.11518268857420111 1108 5 0.18233806234320582 1108 6 0.20843960283654184 1108 7 0.18605617942234431 1108 8 0.13937597596810036 1108 9 0.04489258430921694 1108 10-11 0.1504940646586448 1108 12-13 0.2218591059980426 1108 14-15 0.1606565287324031 1108 16-17 0.2040916401926438 1108 18-19 0.056709747718066694 1108 20-21 0.11373845028307272 1108 22-23 0.2853526041191614 1108 24-25 0.36137153427883817 1108 26-27 0.2620425856229076 1108 28-29 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2315 20-21 -1.1593477978060562 2315 22-23 -1.249698810446823 2315 24-25 -2.4105520846284705 2315 26-27 -1.408785325624251 2315 28-29 -1.5302022131808926 2315 30-31 -2.469331355572862 2315 32-33 -2.2452713803320137 2315 34-35 -1.7277796837663075 2315 36-37 -1.7254949374290263 2315 38-39 -1.808498106210891 2315 40-41 -2.1060686795347223 2315 42-43 -2.204816066790375 2315 44-45 -2.318916541654186 2315 46-47 -2.852889017895045 2315 48-49 -4.067184635781388 2315 50-51 -3.3371226693710447 2315 52-53 -2.906310189184385 2315 54-55 -2.398120086926415 2315 56-57 -2.315294990645331 2315 58-59 -2.5289023187061694 2315 60-61 -2.4511722447112625 2315 62-63 -2.0300044010226053 2315 64-65 -1.9089441763316408 2315 66-67 -1.676124448112862 2315 68-69 -1.5784679568063318 2315 70-71 -1.647649879760774 2315 72-73 -1.6638294058109793 2315 74-75 -1.8494583621821512 2315 76-77 -1.8089912213805803 2315 78-79 -1.7738832885241749 2315 80-81 -1.741394354218361 2315 82-83 -1.8735623734166929 2315 84-85 -1.6523492973814164 2315 86-87 -1.6548137081759293 2315 88-89 -1.8436612970253101 2315 90-91 -1.9130734397911304 2315 92-93 -1.9071960498327556 2315 94-95 -2.059058698607682 2315 96-97 -2.051602722833202 2315 98-99 -2.2643241928679636 2315 100-101 -2.1892736810803513 2316 1 -1.4000723654924982 2316 2 -1.3008607049446361 2316 3 -2.536834643833309 2316 4 -1.634443608563977 2316 5 -1.3539064066106548 2316 6 -2.816679736190345 2316 7 -6.962283568100869 2316 8 -7.76939534505571 2316 9 -4.527461529263533 2316 10-11 -2.420778038767054 2316 12-13 -2.312501027360611 2316 14-15 -2.2959530155633203 2316 16-17 -2.3055034036870836 2316 18-19 -1.7977804359937366 2316 20-21 -2.981528245368864 2316 22-23 -3.5479690081388284 2316 24-25 -2.2773157808512536 2316 26-27 -2.501263177876723 2316 28-29 -3.3009052473974307 2316 30-31 -3.8449599171170377 2316 32-33 -3.922836591659056 2316 34-35 -3.1093741825552215 2316 36-37 -4.13233876607292 2316 38-39 -3.6311207382369304 2316 40-41 -4.318147714653318 2316 42-43 -3.6567777500061 2316 44-45 -3.3496661021985332 2316 46-47 -3.134773676816188 2316 48-49 -3.131420370163454 2316 50-51 -2.7651211131840014 2316 52-53 -2.9837219685591627 2316 54-55 -4.600651406067321 2316 56-57 -3.3058598473259018 2316 58-59 -2.6768704038327833 2316 60-61 -3.4090208593415277 2316 62-63 -3.0383836802251984 2316 64-65 -4.471786742265174 2316 66-67 -3.4838091872289034 2316 68-69 -3.4880743471302758 2316 70-71 -3.853094585965149 2316 72-73 -3.4684924636330727 2316 74-75 -3.1431135315712204 2316 76-77 -3.6774619979407817 2316 78-79 -4.8284915170286204 2316 80-81 -3.747079693277403 2316 82-83 -3.406884022258801 2316 84-85 -3.532341009455603 2316 86-87 -4.64201694302788 2316 88-89 -5.120041434481752 2316 90-91 -4.512751034019214 2316 92-93 -4.684787007046573 2316 94-95 -4.78376321359239 2316 96-97 -4.980841583492701 2316 98-99 -5.092405518408608 2316 100-101 -5.128406656023433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99505.0 3 17107.0 4 14612.0 5 13982.0 6 15783.0 7 19539.0 8 24193.0 9 28591.0 10 33141.0 11 39619.0 12 41004.0 13 46261.0 14 51840.0 15 61826.0 16 77976.0 17 103053.0 18 147067.0 19 214629.0 20 275520.0 21 204732.0 22 124259.0 23 111258.0 24 119285.0 25 127110.0 26 140380.0 27 157960.0 28 180675.0 29 211319.0 30 247781.0 31 294931.0 32 357069.0 33 439734.0 34 563655.0 35 773383.0 36 1099351.0 37 1154370.0 38 817103.0 39 124752.0 40 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.598322474756085 48.03008952312716 24.196804687131454 17.174783314985305 2 16.335568261674705 49.7378132152227 18.04432114270543 15.882297380397164 3 44.78215576648698 20.32548942554557 18.855581243875868 16.03677356409158 4 16.42401417976759 18.745549561715 19.80260015324683 45.02783610527058 5 16.451478390155103 47.42093932134209 20.679075831336906 15.448506457165898 6 45.09870639653308 19.859279680613408 18.62291964371801 16.419094279135493 7 17.23901233599404 19.321429271859756 48.059969050280685 15.379589341865518 8 44.60502224071805 20.04595811939422 19.493164940853873 15.855854699033852 9 44.2712040645428 19.695292359045197 20.045976603619064 15.987526972792935 10-11 16.059681664641047 33.77435891330718 33.83985446832817 16.326104953723593 12-13 15.887591422154562 20.048621157608597 33.77994050761791 30.283846912618927 14-15 16.07162167469608 48.09773418857732 19.4941402193418 16.3365039173848 16-17 30.174593771473813 19.837345201816497 19.630875655418123 30.357185371291568 18-19 16.220046717321345 33.81571216958039 19.627605779000216 30.336635334098048 20-21 30.33842507296342 33.878267702670165 19.65896976772423 16.124337456642184 22-23 30.304076560870357 19.757324966848895 33.74753864439872 16.19105982788203 24-25 16.128923670411275 33.685455992922655 20.09029046429412 30.095329872371945 26-27 16.325005841116383 25.348508276592103 41.895782816062734 16.43070306622878 28-29 27.714523207151803 19.639033179343567 36.35690660608042 16.2895370074242 30-31 25.732480286539722 20.74073901230715 36.70479321101533 16.8219874901378 32-33 19.843292499256577 23.423092924463987 38.00674093940138 18.726873636878054 34-35 19.438897551533007 22.51920854784755 38.686415628141276 19.35547827247816 36-37 18.908990305595104 22.631660688127486 39.02069731291287 19.438651693364534 38-39 19.298054206100616 22.424512431328132 38.758245262704285 19.519188099866966 40-41 19.17067886220082 22.361061308323666 38.64335612275177 19.82490370672374 42-43 19.83042595662519 22.59075020441239 38.549822803700806 19.02900103526161 44-45 19.35035361556926 22.749058261974696 38.51966973477315 19.380918387682893 46-47 19.76152571456175 22.379000970554554 38.48300152689071 19.376471787992987 48-49 19.416165902233 22.55752563154309 38.68971297362304 19.336595492600868 50-51 19.737774181372032 22.603816559192605 37.93385517517795 19.72455408425741 52-53 20.01578659510388 23.788905337310123 36.71859344121229 19.476714626373706 54-55 20.730748912916336 24.19225899004407 35.41849986392312 19.658492233116476 56-57 20.64166412160745 25.0480498429064 34.03784656195316 20.272439473532987 58-59 20.787586198849514 25.73271647433579 33.15581134849422 20.32388597832048 60-61 21.0008457330327 25.972696538421758 32.64632181970806 20.38013590883748 62-63 20.97145242243493 25.880628980442992 32.709986582058605 20.437932015063474 64-65 20.3834917043305 25.578168926613298 33.048311628242644 20.99002774081356 66-67 20.4713555724128 25.18309113407614 33.780908560929966 20.56464473258109 68-69 20.425298665678202 25.19023445808483 33.80179797362485 20.582668902612113 70-71 20.30245729928287 24.908235821526485 34.51767149232139 20.271635386869256 72-73 19.932823221521772 24.898975225974166 34.38976182725504 20.77843972524902 74-75 20.30031977138318 25.07970948712665 33.56565161090989 21.054319130580275 76-77 20.870446815543346 25.496963736701073 33.07284523428528 20.559744213470303 78-79 20.666454231946975 25.61335566425949 32.916695642109026 20.803494461684508 80-81 20.349492833072617 25.993177955345693 32.79662933796576 20.860699873615925 82-83 21.21162801686346 26.073009656179796 32.21690791074057 20.498454416216173 84-85 20.771801663397742 26.587258780986705 31.619474795047743 21.021464760567817 86-87 20.546604770105194 27.080095697782912 31.397470124197437 20.975829407914453 88-89 20.796342516518614 26.50587487309642 31.464463554737815 21.233319055647158 90-91 20.634109466500476 26.617834716689398 31.601387638036627 21.1466681787735 92-93 20.7171629661965 26.95512644570231 31.39069709133087 20.93701349677032 94-95 20.920340478875133 26.44965692625338 31.024238013187173 21.605764581684316 96-97 20.61253923121546 26.193972020389005 31.495923686773253 21.697565061622285 98-99 21.122648110248875 26.602601176721414 30.99576834110163 21.27898237192808 100-101 21.477103983604078 25.998913603353003 30.86275793681693 21.66122447622599 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13060.0 1 7904.0 2 2026.0 3 1168.0 4 973.0 5 871.5 6 965.0 7 1176.5 8 1106.5 9 1091.5 10 1550.5 11 9994.5 12 192470.5 13 216546.0 14 59323.5 15 53059.0 16 55253.5 17 57738.0 18 59370.0 19 59579.5 20 59641.5 21 59800.0 22 60899.5 23 63184.0 24 66448.0 25 71344.5 26 77459.0 27 84571.5 28 91815.5 29 99283.5 30 106056.0 31 114035.5 32 124766.5 33 137607.0 34 155842.0 35 168266.0 36 175600.0 37 185911.0 38 194432.5 39 204374.5 40 218074.0 41 226611.0 42 229428.0 43 236095.0 44 248602.0 45 258956.0 46 261825.5 47 263914.0 48 265083.0 49 266989.0 50 269880.0 51 274101.5 52 275547.5 53 271958.0 54 261300.5 55 242614.0 56 226182.5 57 211485.0 58 215049.0 59 191461.0 60 144096.0 61 113220.5 62 82962.0 63 62044.0 64 45792.5 65 32452.5 66 22200.0 67 15262.0 68 9991.0 69 6418.5 70 4397.0 71 3372.5 72 2839.0 73 2478.0 74 2215.0 75 2074.5 76 1998.0 77 1847.5 78 1689.0 79 1576.0 80 1487.5 81 1376.5 82 1207.5 83 1080.5 84 999.5 85 893.5 86 770.5 87 641.0 88 517.0 89 399.0 90 283.5 91 216.5 92 166.0 93 114.0 94 71.0 95 41.0 96 27.0 97 21.5 98 18.5 99 13.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12159352123221609 2 0.10954612938175771 3 0.10460121733471574 4 0.09211064940456962 5 0.09712553662208859 6 0.08802876446007742 7 0.09700891133796025 8 0.09300866409235789 9 0.08751561320991269 10-11 0.07829055323536033 12-13 0.0697185948519267 14-15 0.07814477163019988 16-17 0.06873894246524857 18-19 0.06147901852825889 20-21 0.05757207150995921 22-23 0.049489939319864666 24-25 0.046871701691183244 26-27 0.043880263153291105 28-29 0.04649850078197253 30-31 0.03769912309448864 32-33 0.05014304091098342 34-35 0.059572195132760394 36-37 0.05098857422091395 38-39 0.05305284174998571 40-41 0.05314614197728839 42-43 0.04866189980255339 44-45 0.04835284279961327 46-47 0.04795631683357689 48-49 0.047501478225476325 50-51 0.048807681407713834 52-53 0.045571329773152154 54-55 0.04603783090966555 56-57 0.045979518267601376 58-59 0.04863274348152131 60-61 0.04430011417615316 62-63 0.05365929322745312 64-65 0.04951326437669034 66-67 0.044393414403455844 68-69 0.04197343975779261 70-71 0.04978150253018554 72-73 0.04431760796877241 74-75 0.04632356285578001 76-77 0.041670214019058904 78-79 0.04658013848086237 80-81 0.04858026210366355 82-83 0.03819478055203412 84-85 0.04805544832508598 86-87 0.05091276778623052 88-89 0.04649850078197253 90-91 0.04253324112160868 92-93 0.039880015907688755 94-95 0.043361280638919954 96-97 0.03140135775155782 98-99 0.03233436002458461 100-101 0.024427165760682584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8574470.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.39129822908453 #Duplication Level Percentage of deduplicated Percentage of total 1 92.14177222147111 46.43143523368528 2 4.538938401579029 4.5744599727482615 3 1.191626993574195 1.8014289363307394 4 0.5357487231454668 1.0798829473549785 5 0.32625878139284853 0.8220301776512362 6 0.21670502429827054 0.6552028504293097 7 0.15546703813506546 0.5483930118419579 8 0.11619060746574568 0.4683996441779908 9 0.0876952213555425 0.39771684473334523 >10 0.6550270485827613 6.020913719470034 >50 0.018289840631345262 0.6020515475676284 >100 0.009573130858790767 1.1270716343507516 >500 0.0029470008755799184 1.0810264840109394 >1k 0.003150242315185083 3.2439243839667573 >5k 5.081035992234005E-4 1.8498377919810436 >10k+ 1.016207198446801E-4 29.296224819699784 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1119966 13.06163529640899 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 24239 0.2826880261987038 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9958 0.1161354579350094 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9534 0.11119054588796741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1662528412834847E-5 0.0 0.0 0.0 0.0 2 2.3325056825669693E-5 0.0 0.0 0.0 0.0 3 2.3325056825669693E-5 0.0 0.0 0.0 0.0 4 3.498758523850454E-5 0.0 0.0 0.0 1.1662528412834847E-5 5 3.498758523850454E-5 0.0 0.0 0.0 1.1662528412834847E-5 6 4.6650113651339386E-5 1.1662528412834847E-5 0.0 0.0 1.1662528412834847E-5 7 4.6650113651339386E-5 1.1662528412834847E-5 0.0 0.0 1.1662528412834847E-5 8 5.831264206417423E-5 1.1662528412834847E-5 0.0 0.0 1.1662528412834847E-5 9 6.997517047700908E-5 1.1662528412834847E-5 0.0 0.0 1.1662528412834847E-5 10-11 1.1079401992193103E-4 1.1662528412834847E-5 0.0 0.0 1.1662528412834847E-5 12-13 1.6910666198610529E-4 1.749379261925227E-5 0.0 0.0 1.1662528412834847E-5 14-15 1.807691903989401E-4 2.3325056825669693E-5 0.0 0.0 2.3325056825669693E-5 16-17 1.8660045460535754E-4 2.3325056825669693E-5 0.0 0.0 2.3325056825669693E-5 18-19 1.9243171881177496E-4 2.3325056825669693E-5 0.0 0.0 2.3325056825669693E-5 20-21 2.0992551143102721E-4 2.3325056825669693E-5 0.0 0.0 2.3325056825669693E-5 22-23 2.274193040502795E-4 2.3325056825669693E-5 0.0 0.0 2.3325056825669693E-5 24-25 2.6823815349520143E-4 2.3325056825669693E-5 0.0 1.1662528412834847E-5 2.3325056825669693E-5 26-27 3.090570029401234E-4 2.3325056825669693E-5 0.0 1.1662528412834847E-5 2.9156321032087114E-5 28-29 3.498758523850454E-4 4.081884944492196E-5 0.0 1.1662528412834847E-5 3.498758523850454E-5 30-31 3.7320090921071504E-4 4.6650113651339386E-5 0.0 2.3325056825669693E-5 3.498758523850454E-5 32-33 4.1985102286205443E-4 4.6650113651339386E-5 0.0 2.3325056825669693E-5 3.498758523850454E-5 34-35 4.839949291326461E-4 4.6650113651339386E-5 0.0 2.3325056825669693E-5 4.6650113651339386E-5 36-37 5.306450427839855E-4 4.6650113651339386E-5 0.0 2.3325056825669693E-5 4.6650113651339386E-5 38-39 5.423075711968203E-4 4.6650113651339386E-5 0.0 3.498758523850454E-5 4.6650113651339386E-5 40-41 5.714638922289074E-4 5.2481377857756804E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 42-43 6.06451477467412E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 44-45 6.181140058802468E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 46-47 6.239452700866643E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 48-49 6.76426647944421E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 50-51 7.638956110406824E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 5.831264206417423E-5 52-53 8.047144604856044E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 9.330022730267876E-5 54-55 8.338707815176915E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 1.1662528412834846E-4 56-57 8.921834235818657E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 1.224565483347659E-4 58-59 9.679898582652922E-4 5.831264206417423E-5 0.0 3.498758523850454E-5 1.282878125411833E-4 60-61 0.0010379650287423011 5.831264206417423E-5 0.0 3.498758523850454E-5 1.282878125411833E-4 62-63 0.0011429277844578149 5.831264206417423E-5 0.0 4.6650113651339386E-5 1.3995034095401815E-4 64-65 0.0012187342191412414 5.831264206417423E-5 0.0 4.6650113651339386E-5 1.3995034095401815E-4 66-67 0.0012945406538246679 5.831264206417423E-5 0.0 4.6650113651339386E-5 1.3995034095401815E-4 68-69 0.0013820096169209293 6.414390627059165E-5 0.0 4.6650113651339386E-5 1.457816051604356E-4 70-71 0.0014811411084300254 6.997517047700908E-5 0.0 4.6650113651339386E-5 1.574441335732704E-4 72-73 0.0016269227135904609 8.163769888984392E-5 0.0 4.6650113651339386E-5 1.6327539777968785E-4 74-75 0.0017493792619252269 8.163769888984392E-5 0.0 4.6650113651339386E-5 1.6327539777968785E-4 76-77 0.0019009921312920799 8.163769888984392E-5 0.0 4.6650113651339386E-5 1.749379261925227E-4 78-79 0.002122580171135942 8.163769888984392E-5 0.0 4.6650113651339386E-5 1.8660045460535754E-4 80-81 0.0024199746456632304 8.163769888984392E-5 0.0 4.6650113651339386E-5 2.040942472246098E-4 82-83 0.0027115378559841013 8.163769888984392E-5 0.0 4.6650113651339386E-5 2.2158803984386206E-4 84-85 0.0031955327851167477 8.163769888984392E-5 0.0 4.6650113651339386E-5 2.3908183246311435E-4 86-87 0.003871959433061169 8.163769888984392E-5 0.0 4.6650113651339386E-5 2.449130966695318E-4 88-89 0.004950743311248392 8.163769888984392E-5 0.0 4.6650113651339386E-5 2.449130966695318E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 243605 0.0 91.807304 1 AGTGGTA 242600 0.0 91.74826 5 GGTATCA 244045 0.0 91.52254 8 TGGTATC 243745 0.0 91.38619 7 GTATCAA 244835 0.0 91.341156 9 GTGGTAT 245100 0.0 90.98621 6 AGCAGTG 246325 0.0 90.718445 2 GCAGTGG 246045 0.0 90.66997 3 CAGTGGT 245870 0.0 90.52926 4 GTATCGA 640 0.0 47.515217 9 TCAACGC 243975 0.0 46.320587 12-13 AACGCAG 244535 0.0 46.25518 14-15 ATCAACG 243645 0.0 46.239258 10-11 CAACGCA 243840 0.0 46.22542 12-13 ACGCAGA 245030 0.0 46.066723 14-15 CGCAGAG 245575 0.0 46.043327 16-17 TATCAAC 245535 0.0 45.95203 10-11 CAGAGTA 243935 0.0 45.752163 18-19 AGAGTAC 239195 0.0 45.734333 18-19 GAGTACT 140060 0.0 45.519585 20-21 >>END_MODULE