FastQCFastQC Report
Sat 18 Jun 2016
SRR578570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574470
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1413331.6483001281711873No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162740.1897959873904743No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103130.12027565552156577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG5200.034.6940653
ACTCGCG3100.029.1035150-51
CGCGTAG3500.025.0958254-55
CGTAGCG3800.024.98748856-57
CGTACGC3850.023.42975820-21
CTCGCGT3800.023.11659252-53
CGCTAGT4250.022.90136790-91
TCGCGTA4600.019.09631752-53
ATCAACG62200.018.58562510-11
ACGCTAG4900.018.41000790-91
CAACGCA63000.018.31188412-13
ACGTACG4800.018.29807520-21
AGTGGTA79300.017.4217665
AAGCAGT82750.017.2971881
GTGTACG10900.016.9866589
GGTATCA81900.016.9265658
GTATCAA86550.016.1268719
TCAACGC63450.015.937366510-11
AACGCAG64600.015.54336912-13
TGGTATC90250.015.5183187
Produced by FastQC (version 0.11.5)