##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578570_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8574470 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.402693810812796 31.0 31.0 34.0 30.0 34.0 2 31.779631977253405 31.0 31.0 34.0 30.0 34.0 3 31.85495721601452 31.0 31.0 34.0 30.0 34.0 4 35.556250473790215 37.0 35.0 37.0 33.0 37.0 5 35.37856229014738 37.0 35.0 37.0 33.0 37.0 6 35.37115121984216 37.0 35.0 37.0 32.0 37.0 7 35.36251686693172 37.0 35.0 37.0 32.0 37.0 8 35.319138908877164 37.0 35.0 37.0 32.0 37.0 9 36.86334852183283 39.0 37.0 39.0 32.0 39.0 10-11 36.85617478398082 39.0 37.0 39.0 32.0 39.0 12-13 36.84638467450466 39.0 37.0 39.0 32.0 39.0 14-15 38.020733118198564 40.0 37.0 41.0 33.0 41.0 16-17 37.951276871923284 40.0 37.0 41.0 33.0 41.0 18-19 37.869125205406284 40.0 37.0 41.0 32.5 41.0 20-21 37.820470536371346 40.0 37.0 41.0 32.0 41.0 22-23 37.617057497431325 39.5 36.0 41.0 32.0 41.0 24-25 37.61642923702573 39.0 36.0 41.0 32.0 41.0 26-27 37.446887912605675 39.0 36.0 41.0 32.0 41.0 28-29 37.29635056160906 39.0 36.0 41.0 31.5 41.0 30-31 37.157409262613314 39.0 36.0 41.0 31.0 41.0 32-33 37.01810630861149 39.0 36.0 40.5 31.0 41.0 34-35 36.867062745569115 39.0 35.5 40.0 30.0 41.0 36-37 36.71271262247113 39.0 35.0 40.0 30.0 41.0 38-39 36.5414068741275 38.0 35.0 40.0 30.0 41.0 40-41 36.38115761090773 38.0 35.0 40.0 30.0 41.0 42-43 36.46239476025923 38.0 35.0 40.0 30.0 41.0 44-45 36.39502313262511 38.0 35.0 40.0 30.0 41.0 46-47 36.24084520675913 38.0 35.0 40.0 30.0 41.0 48-49 36.09023788059204 38.0 35.0 40.0 29.0 41.0 50-51 35.897639270998674 38.0 34.5 40.0 28.5 41.0 52-53 35.94205770152558 38.0 35.0 40.0 28.0 41.0 54-55 35.928245302625115 38.0 34.5 40.0 28.0 41.0 56-57 35.7661652556951 38.0 34.0 40.0 28.0 41.0 58-59 35.53458132106124 38.0 34.0 40.0 28.0 41.0 60-61 35.27173673708113 38.0 34.0 40.0 28.0 41.0 62-63 34.989044045871054 37.0 34.0 40.0 27.5 41.0 64-65 34.66570009574936 37.0 33.0 40.0 26.5 41.0 66-67 34.32103511937181 36.0 33.0 39.0 26.0 41.0 68-69 33.941931396342866 36.0 32.5 39.0 26.0 40.5 70-71 33.54510943533536 35.0 32.0 39.0 26.0 40.0 72-73 33.153083630824995 35.0 32.0 38.0 26.0 40.0 74-75 32.73906672948882 35.0 31.5 37.0 25.0 39.5 76-77 31.368282237852604 34.0 29.5 36.0 23.0 39.0 78-79 32.025434341714416 34.5 31.0 36.0 24.5 39.0 80-81 31.90105959901895 35.0 31.0 36.0 24.0 38.5 82-83 31.53843333757072 34.0 31.0 35.5 23.5 37.0 84-85 31.258090529210552 34.0 31.0 35.0 23.0 37.0 86-87 30.942312527771396 34.0 31.0 35.0 21.5 36.5 88-89 30.631780098361766 34.0 30.5 35.0 20.0 36.0 90-91 30.289923925327162 34.0 30.0 35.0 19.5 36.0 92-93 29.916561606723214 34.0 30.0 35.0 18.0 35.5 94-95 29.528475520935988 34.0 29.5 35.0 11.0 35.0 96-97 29.057595046690935 34.0 29.0 35.0 2.0 35.0 98-99 28.23416257797858 34.0 29.0 35.0 2.0 35.0 100-101 27.16848271671602 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34265669444395286 1101 2 0.24963757954718702 1101 3 0.3089241693363256 1101 4 0.25128232293030806 1101 5 0.2732620139417534 1101 6 0.14514022633571955 1101 7 0.21522240874870135 1101 8 0.20652889021381782 1101 9 0.33223489521500227 1101 10-11 0.309562163642056 1101 12-13 0.2798340242674442 1101 14-15 0.24180570687601488 1101 16-17 0.31126797983972665 1101 18-19 0.3490501453453234 1101 20-21 0.2908811609162498 1101 22-23 0.4183731411331095 1101 24-25 0.38001777366012135 1101 26-27 0.33570967857436074 1101 28-29 0.302120898026466 1101 30-31 0.3301192032598266 1101 32-33 0.378344705279936 1101 34-35 0.3884495205995435 1101 36-37 0.4017202108872411 1101 38-39 0.2623947961088504 1101 40-41 0.29326901448069265 1101 42-43 0.2821053245640641 1101 44-45 0.302454320248394 1101 46-47 0.3023961389485521 1101 48-49 0.35786327982245325 1101 50-51 0.35736470555743693 1101 52-53 0.40799663670437525 1101 54-55 0.4247340691056394 1101 56-57 0.5239951138864072 1101 58-59 0.4725451555767606 1101 60-61 0.3913802197021141 1101 62-63 0.31391189233688976 1101 64-65 0.33958356980961923 1101 66-67 0.37381116886955823 1101 68-69 0.27280544890207636 1101 70-71 0.34712422884631877 1101 72-73 0.4425539074691116 1101 74-75 0.4360946011854665 1101 76-77 0.37541507439571475 1101 78-79 0.336385532268757 1101 80-81 0.3179997284373677 1101 82-83 0.46601413676468795 1101 84-85 0.4605527788965098 1101 86-87 0.4192396760641195 1101 88-89 0.4801989289801014 1101 90-91 0.38457530423898234 1101 92-93 0.5344539072163492 1101 94-95 0.40690853950302497 1101 96-97 0.4400034073718331 1101 98-99 0.7852891949820098 1101 100-101 0.9652181025845863 1102 1 0.3042671939601398 1102 2 0.3346857171537785 1102 3 0.316868630650621 1102 4 0.3212614408286356 1102 5 0.35124935551311154 1102 6 0.1999192023762575 1102 7 0.30114492062872245 1102 8 0.15223253659360836 1102 9 0.2425114555940766 1102 10-11 0.30872680875963 1102 12-13 0.31510501854101136 1102 14-15 0.39792039244346 1102 16-17 0.24539244135796423 1102 18-19 0.32690915490145755 1102 20-21 0.321812419938162 1102 22-23 0.4939387735899956 1102 24-25 0.3442771162194944 1102 26-27 0.5048983670016227 1102 28-29 0.3408781297146888 1102 30-31 0.39275637306088385 1102 32-33 0.3829630149060961 1102 34-35 0.3289708536549085 1102 36-37 0.40567410030614326 1102 38-39 0.3932773340036917 1102 40-41 0.4095779727096982 1102 42-43 0.35565849121071125 1102 44-45 0.389008053986295 1102 46-47 0.3500241565425455 1102 48-49 0.34634418907198494 1102 50-51 0.34576828418087047 1102 52-53 0.42886321559747387 1102 54-55 0.45484667156163994 1102 56-57 0.4631851704709007 1102 58-59 0.47974499094467404 1102 60-61 0.4733578499064919 1102 62-63 0.30021018007253986 1102 64-65 0.2371799894866129 1102 66-67 0.28626506791938766 1102 68-69 0.2891179337475478 1102 70-71 0.36429790210204516 1102 72-73 0.4199787183065027 1102 74-75 0.3107105682318547 1102 76-77 0.20652696982884677 1102 78-79 0.25116712565355925 1102 80-81 0.4222057355257256 1102 82-83 0.49811288995705283 1102 84-85 0.4442918053124245 1102 86-87 0.4066274333513249 1102 88-89 0.3786915199696388 1102 90-91 0.38649531321273756 1102 92-93 0.4821231111584545 1102 94-95 0.4630065267539507 1102 96-97 0.5841165497259055 1102 98-99 0.9833345855101712 1102 100-101 1.099951804801055 1103 1 0.2501845454921252 1103 2 0.26479670585989723 1103 3 0.21294257539667072 1103 4 0.2230988825767426 1103 5 0.26697122643619764 1103 6 0.10382800542866022 1103 7 0.2717455920931826 1103 8 0.08927655151278913 1103 9 0.15696239605162532 1103 10-11 0.17383072721732873 1103 12-13 0.27621318038202247 1103 14-15 0.3538665392437821 1103 16-17 0.3067619644525337 1103 18-19 0.2919346018987383 1103 20-21 0.397443678403981 1103 22-23 0.34094217040858865 1103 24-25 0.27454980660159123 1103 26-27 0.23576584691714686 1103 28-29 0.24857422299616871 1103 30-31 0.3499951870035787 1103 32-33 0.30803730702756127 1103 34-35 0.2793909543622348 1103 36-37 0.33869099470964414 1103 38-39 0.3375608417081395 1103 40-41 0.3166031226619097 1103 42-43 0.27626296830786856 1103 44-45 0.26449167447461974 1103 46-47 0.3576374790271899 1103 48-49 0.2928231285635192 1103 50-51 0.203079775422502 1103 52-53 0.19568584692026292 1103 54-55 0.37992775896598374 1103 56-57 0.3464952220176798 1103 58-59 0.28429912644686084 1103 60-61 0.320217009817533 1103 62-63 0.36215704164610685 1103 64-65 0.2509824158483056 1103 66-67 0.21746079908290739 1103 68-69 0.3841658456345982 1103 70-71 0.3227826047614428 1103 72-73 0.35735988485429715 1103 74-75 0.29403894852852375 1103 76-77 0.2640510965115759 1103 78-79 0.3632227940371777 1103 80-81 0.3687762433397559 1103 82-83 0.5587882450774408 1103 84-85 0.36936097525799383 1103 86-87 0.32102944228654096 1103 88-89 0.45468580150938465 1103 90-91 0.447193872407059 1103 92-93 0.5015457713080487 1103 94-95 0.5968962275873366 1103 96-97 0.5693753594913069 1103 98-99 1.079190957778117 1103 100-101 0.9741128959301228 1104 1 0.22768752448146756 1104 2 0.20833834229884118 1104 3 0.15862131710822425 1104 4 0.17802754510734786 1104 5 0.17458619313371315 1104 6 0.17246634992681464 1104 7 0.18124814846263604 1104 8 0.054156004974757366 1104 9 0.19979413475218166 1104 10-11 0.15921172659496108 1104 12-13 0.2050575629299729 1104 14-15 0.30482940274622194 1104 16-17 0.3421566285133224 1104 18-19 0.1391164038335262 1104 20-21 0.2262080447576622 1104 22-23 0.3287182301971754 1104 24-25 0.2610801169532593 1104 26-27 0.3270394533924019 1104 28-29 0.30274762862248394 1104 30-31 0.2717743838084914 1104 32-33 0.28634755458612915 1104 34-35 0.2789799354821696 1104 36-37 0.24608344562894047 1104 38-39 0.23926456198628898 1104 40-41 0.39191302214982926 1104 42-43 0.23144661204449335 1104 44-45 0.25006997148818044 1104 46-47 0.2368931142170041 1104 48-49 0.257826750397804 1104 50-51 0.26760525190171336 1104 52-53 0.2317597330094614 1104 54-55 0.293478661079547 1104 56-57 0.2262900565892778 1104 58-59 0.21478440294333012 1104 60-61 0.2109946032308585 1104 62-63 0.18573939121848326 1104 64-65 0.31122515517004246 1104 66-67 0.22875423338116008 1104 68-69 0.25650592942366757 1104 70-71 0.29573483099024855 1104 72-73 0.3398230078591169 1104 74-75 0.3053995374335656 1104 76-77 0.21038732509304836 1104 78-79 0.32900463501621857 1104 80-81 0.46997899266247956 1104 82-83 0.4356186920464715 1104 84-85 0.3485903621460764 1104 86-87 0.5219020864616049 1104 88-89 0.4740094023053736 1104 90-91 0.43628862335087604 1104 92-93 0.47545210736328514 1104 94-95 0.48675802399597856 1104 96-97 0.5462882406491261 1104 98-99 0.840168810301325 1104 100-101 0.8941174388126747 1105 1 0.2093208422456172 1105 2 0.1715230518573101 1105 3 0.10530819730756846 1105 4 0.15594331818666518 1105 5 0.17823919163830482 1105 6 0.07013134187045722 1105 7 0.17426766363366397 1105 8 0.016684665926824493 1105 9 0.2455291536106614 1105 10-11 0.08923546436275132 1105 12-13 0.08837887737832517 1105 14-15 0.13596203517502659 1105 16-17 0.09664784299123141 1105 18-19 0.14402147134696008 1105 20-21 0.0981566580516855 1105 22-23 0.261838422285372 1105 24-25 0.23864484007155085 1105 26-27 0.16094834058883123 1105 28-29 0.1863416237667579 1105 30-31 0.10508277748554207 1105 32-33 0.19739363873880933 1105 34-35 0.17431137665713692 1105 36-37 0.08258296605697524 1105 38-39 0.14385163189961503 1105 40-41 0.15413910173403877 1105 42-43 0.22376309165582597 1105 44-45 0.2435207451182393 1105 46-47 0.2445988527953844 1105 48-49 0.23153988912654455 1105 50-51 0.24439121048772705 1105 52-53 0.2824155201500034 1105 54-55 0.37419180742155334 1105 56-57 0.3243463808986107 1105 58-59 0.1962096887274427 1105 60-61 0.14867126731815716 1105 62-63 0.16740303389575217 1105 64-65 0.19508362175120908 1105 66-67 0.3428604729588969 1105 68-69 0.2767112806096179 1105 70-71 0.3403417848696435 1105 72-73 0.3849032469896443 1105 74-75 0.4122013757128826 1105 76-77 0.20511251409120845 1105 78-79 0.37511342829512984 1105 80-81 0.5056423145057529 1105 82-83 0.5202460912745188 1105 84-85 0.4167233413247544 1105 86-87 0.49584740431168584 1105 88-89 0.4846590798602932 1105 90-91 0.6896785602835145 1105 92-93 0.8099603217362734 1105 94-95 0.7629613507696753 1105 96-97 0.7485401012799286 1105 98-99 1.194216080099153 1105 100-101 1.3001563235541447 1106 1 0.13974099933712125 1106 2 0.11149099901554393 1106 3 0.08407455544126918 1106 4 0.06573321434828472 1106 5 0.1071296505811361 1106 6 0.00367529029049507 1106 7 0.04887037967747432 1106 8 0.03764838486583244 1106 9 0.053908611423722164 1106 10-11 -0.004256294742567945 1106 12-13 0.05328772072316923 1106 14-15 0.006924446485164992 1106 16-17 0.04431257716622383 1106 18-19 0.07202512016266382 1106 20-21 0.09580550592693982 1106 22-23 0.18581423271935193 1106 24-25 0.09770780333764861 1106 26-27 0.12756860961538052 1106 28-29 0.1648897823976725 1106 30-31 0.19499145642830484 1106 32-33 0.12734853793605794 1106 34-35 0.18089163325185353 1106 36-37 0.12088204996810248 1106 38-39 0.176342023483123 1106 40-41 0.22249141752200785 1106 42-43 0.18294864323189586 1106 44-45 0.05369734532970227 1106 46-47 0.09970709866765048 1106 48-49 0.0712596447835736 1106 50-51 0.12771963030650824 1106 52-53 0.16238732999840266 1106 54-55 0.1872182671527085 1106 56-57 0.1389311700334659 1106 58-59 0.07367112935708775 1106 60-61 0.2598486184171662 1106 62-63 0.22081157974869825 1106 64-65 0.11512045945236338 1106 66-67 0.08512884534690812 1106 68-69 0.14361585648107678 1106 70-71 0.27186164013915004 1106 72-73 0.3571815907433731 1106 74-75 0.38778733761631656 1106 76-77 0.13672291696938998 1106 78-79 0.33701346059887527 1106 80-81 0.41810523677756706 1106 82-83 0.6008028842016451 1106 84-85 0.5375003450244584 1106 86-87 0.6120728351791911 1106 88-89 0.6212010545448017 1106 90-91 0.6650566926616897 1106 92-93 0.6380098817305431 1106 94-95 0.6584436877640023 1106 96-97 0.7440862465164706 1106 98-99 1.2034101527616343 1106 100-101 1.223803329822566 1107 1 0.17692563486835056 1107 2 0.0637747312796293 1107 3 0.0253156231831575 1107 4 -0.0018923383726345833 1107 5 0.028111553468221473 1107 6 0.0171024482841986 1107 7 -0.029752484662502354 1107 8 -0.004641867165851465 1107 9 0.0566521678965799 1107 10-11 0.012483716325284888 1107 12-13 0.0487076722352171 1107 14-15 -0.022113568302906117 1107 16-17 0.027052337859437614 1107 18-19 -0.030890669907158497 1107 20-21 0.02074032487779931 1107 22-23 0.1535915769771492 1107 24-25 0.15094860080386496 1107 26-27 0.07267142078426048 1107 28-29 0.038036471694574914 1107 30-31 0.05759371724878548 1107 32-33 0.06418437562105339 1107 34-35 0.16194394236435272 1107 36-37 0.22929925206590696 1107 38-39 0.18911974350750427 1107 40-41 0.18577159169353052 1107 42-43 0.11992075065928987 1107 44-45 0.1749660842258507 1107 46-47 0.12777633784239129 1107 48-49 0.17254765444288722 1107 50-51 0.14657852107936975 1107 52-53 0.09470479837632695 1107 54-55 0.08186455390102765 1107 56-57 0.1394136233712615 1107 58-59 0.13046962929895045 1107 60-61 0.2006616025875445 1107 62-63 0.2698558587626252 1107 64-65 0.21339645951968578 1107 66-67 0.20004373971608658 1107 68-69 0.25853185077966145 1107 70-71 0.19796706535421293 1107 72-73 0.17831814370479293 1107 74-75 0.2648830676443268 1107 76-77 0.14665605967898898 1107 78-79 0.24590664691479702 1107 80-81 0.2453826444475773 1107 82-83 0.3872463335823042 1107 84-85 0.4027278367725593 1107 86-87 0.47392042931895517 1107 88-89 0.34362412330793646 1107 90-91 0.3252116322988421 1107 92-93 0.46571392132103995 1107 94-95 0.4344594354788711 1107 96-97 0.422467809366772 1107 98-99 0.9622403732350691 1107 100-101 1.07112005076009 1108 1 0.05249427143867891 1108 2 -0.04321587565521412 1108 3 -0.05093389285924843 1108 4 -0.06725976263517452 1108 5 0.02036100578101241 1108 6 0.017740702051149526 1108 7 -0.09630587166046212 1108 8 0.01522087562588581 1108 9 0.01697273827308976 1108 10-11 -0.05297137967962584 1108 12-13 -0.1191236849433821 1108 14-15 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-1.2898266914503012 2316 90-91 -1.3899308327727908 2316 92-93 -1.3708784307209037 2316 94-95 -1.5184596951557374 2316 96-97 -1.7142837405350662 2316 98-99 -1.2864356007907887 2316 100-101 -1.6594870107905386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115.0 3 91.0 4 243.0 5 625.0 6 1227.0 7 2033.0 8 3643.0 9 5536.0 10 7780.0 11 10026.0 12 12476.0 13 15073.0 14 17479.0 15 20099.0 16 23477.0 17 27481.0 18 32001.0 19 36468.0 20 38919.0 21 41610.0 22 46544.0 23 55426.0 24 69333.0 25 86692.0 26 110516.0 27 139808.0 28 176538.0 29 217138.0 30 265486.0 31 323128.0 32 392883.0 33 489428.0 34 630578.0 35 883625.0 36 1123412.0 37 1549381.0 38 1410938.0 39 306716.0 40 498.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.49669392014221 30.740450916468347 29.182980963217798 22.57987420017164 2 22.28165705868701 29.590540289953783 27.673103993599607 20.454698657759604 3 21.43147039991976 28.565404042465598 28.150637881991543 21.8524876756231 4 21.794144525963553 28.482329500685992 28.154073449303297 21.569452524047154 5 22.305333684610368 28.574474171982768 28.28295621784261 20.837235925564258 6 22.247772277978285 28.635852498596414 27.348458572405892 21.767916651019412 7 22.394817537716964 28.756911370597578 27.81783149650176 21.030439595183694 8 21.623680530691693 28.876746900974638 27.86217690422848 21.637395664105185 9 21.358173784806148 28.565010837423486 28.3916587118365 21.685156665933867 10-11 21.399695770538724 28.983629152147266 27.841130083110883 21.775544994203123 12-13 21.384575373171753 29.10158878624568 27.698983144147686 21.81485269643488 14-15 21.549069505170582 29.138623145220638 27.507303658418536 21.80500369119024 16-17 21.700070091795762 28.929998005707642 27.538127721013662 21.831804181482937 18-19 21.67135694684336 28.956617726809935 27.498043610858748 21.873981715487957 20-21 21.7692522103407 28.876735238446226 27.550490001131266 21.803522550081812 22-23 21.83336216909794 28.8360036884121 27.513838688709203 21.81679545378076 24-25 21.719324926205353 28.835834751302414 27.46812339421562 21.976716928276616 26-27 21.89360502375367 28.75358987412043 27.567924062900715 21.784881039225187 28-29 21.935244550625775 28.791737170416837 27.49478890395768 21.778229374999707 30-31 21.89942554038916 28.808481778649647 27.45289472011495 21.839197960846242 32-33 21.910654209051742 28.852973965360622 27.372373610422855 21.863998215164777 34-35 21.919094383122033 28.857703546202103 27.386725727758048 21.836476342917816 36-37 21.94493025547103 28.870476145603046 27.319371209954802 21.865222388971123 38-39 21.945352770778243 28.85850145631555 27.308878848121694 21.887266924784516 40-41 21.93657194160447 28.897556605162126 27.314887491780592 21.850983961452812 42-43 21.965493769246688 28.92789897140482 27.276038941428254 21.83056831792024 44-45 21.975158871007903 28.90801570114045 27.280888980220098 21.83593644763155 46-47 21.9758173744353 28.95932831936672 27.22536052768242 21.83949377851556 48-49 21.971889139298433 28.943077008759023 27.225027139580106 21.86000671236244 50-51 22.041453935393456 28.938933304532434 27.19537274344673 21.82424001662739 52-53 21.966518155648405 29.013292390303825 27.1746119468505 21.845577507197273 54-55 21.98430409852719 29.01178195647798 27.11316057298158 21.890753372013254 56-57 22.03152505613813 29.053987072660366 27.06459098062532 21.849896890576186 58-59 21.981850161991936 29.140978848942016 27.071684121932794 21.805486867133254 60-61 21.998667089549972 29.107334278814566 27.041008192985 21.852990438650462 62-63 21.99376275626904 29.150632859058597 27.03427813330063 21.821326251371733 64-65 21.961376523700533 29.195477489290354 27.05393901483329 21.789206972175826 66-67 21.97523646313361 29.227969925759073 27.024493080025252 21.77230053108207 68-69 21.94464222714006 29.265782038609906 27.009094116377863 21.78048161787217 70-71 21.98345112238058 29.303018702115246 26.974932931823275 21.738597243680896 72-73 21.95035657654559 29.293656476690977 26.973627313989073 21.782359632774355 74-75 21.990576462733 29.321651101696645 26.95185307212563 21.735919363444722 76-77 21.939211612329117 29.399632741198005 26.957886529975795 21.703269116497083 78-79 21.969331757851755 29.41683525652999 26.889255630438814 21.72457735517945 80-81 21.93531292043523 29.38543682345499 26.93467847726174 21.744571778848037 82-83 22.00225336980602 29.457016717284194 26.839376812217065 21.701353100692717 84-85 21.923382032976054 29.471358209679344 26.883406356362332 21.721853400982265 86-87 21.905073466510153 29.555801250595525 26.83170025827136 21.70742502462296 88-89 21.937208800564466 29.593431648677175 26.803573407040687 21.66578614371767 90-91 21.904037432422268 29.555589118053366 26.822186080984235 21.718187368540136 92-93 21.88711608159594 29.613120437845534 26.799233014568568 21.70053046598995 94-95 21.900898904067237 29.65996222119941 26.794643184634047 21.64449569009931 96-97 21.88817478486202 29.683396387447676 26.758456383032197 21.669972444658104 98-99 21.930126724159663 29.69394975234944 26.692972516034708 21.68295100745619 100-101 21.95436513027305 29.66227462699657 26.680713822877543 21.702646419852837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 153567.0 1 86898.0 2 14828.5 3 8452.5 4 7288.0 5 6431.5 6 5781.0 7 5683.0 8 5607.5 9 5281.5 10 4804.0 11 5237.5 12 9337.0 13 9188.5 14 5471.0 15 5818.5 16 6546.5 17 7723.5 18 10367.0 19 12512.0 20 14854.0 21 19079.0 22 24306.5 23 30174.5 24 36719.5 25 44956.0 26 57716.0 27 71803.5 28 81379.5 29 90069.0 30 102135.0 31 118858.0 32 137439.0 33 157481.5 34 178090.0 35 194797.5 36 210120.0 37 224055.5 38 236627.0 39 246368.5 40 257185.5 41 268258.0 42 273325.0 43 279507.5 44 289336.0 45 300829.5 46 309432.5 47 311476.0 48 313325.5 49 318611.5 50 319911.5 51 314691.0 52 306098.5 53 292648.5 54 272756.0 55 252616.5 56 231764.5 57 209354.5 58 191975.5 59 170675.5 60 140602.5 61 108911.5 62 80685.0 63 58906.0 64 42694.5 65 29483.0 66 19779.0 67 14046.5 68 9005.0 69 4822.0 70 2361.0 71 1061.0 72 497.5 73 251.0 74 138.5 75 97.0 76 71.0 77 51.0 78 36.0 79 23.5 80 18.5 81 18.0 82 15.0 83 13.5 84 11.5 85 5.5 86 4.0 87 3.5 88 1.0 89 0.5 90 1.5 91 3.0 92 2.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16009152752298392 2 0.0 3 0.0 4 7.697268752470998E-4 5 4.7816366492622864E-4 6 1.8660045460535752E-4 7 1.51612869366853E-4 8 0.0 9 1.7493792619252267E-4 10-11 2.449130966695318E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.6563262802249E-4 24-25 0.0 26-27 5.8312642064174234E-5 28-29 0.005341438013078359 30-31 0.003790321734171325 32-33 1.0496275571551362E-4 34-35 8.338707815176915E-4 36-37 3.965259660363848E-4 38-39 0.001072952613980806 40-41 0.002938957160034381 42-43 0.0012303967475540763 44-45 7.114142331829256E-4 46-47 0.008776052630658221 48-49 0.008601114704465698 50-51 0.007743918866122338 52-53 0.014653966950726982 54-55 0.005971214547371441 56-57 9.796523866781271E-4 58-59 0.0014403222589851035 60-61 5.8312642064174224E-6 62-63 1.1079401992193104E-4 64-65 8.571958383433611E-4 66-67 0.0010379650287423011 68-69 0.002857319461144537 70-71 0.002011786151214011 72-73 0.00588374558427518 74-75 0.002274193040502795 76-77 0.0015744413357327042 78-79 0.0018718358102599926 80-81 2.565756250823666E-4 82-83 0.0023383369467733866 84-85 6.997517047700908E-5 86-87 0.001807691903989401 88-89 2.3325056825669694E-4 90-91 9.913149150909619E-4 92-93 0.0014228284663658512 94-95 9.854836508845443E-4 96-97 0.0010379650287423011 98-99 0.5284874750276111 100-101 2.0992551143102724E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8574470.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.1260617089601 #Duplication Level Percentage of deduplicated Percentage of total 1 91.48045098276995 64.15163750781225 2 4.937576275050223 6.925055571137386 3 1.2131975519997638 2.552302991900843 4 0.5541389458363919 1.5543832764424366 5 0.33025248782141853 1.1579653170251194 6 0.21867587903953473 0.9200926912672505 7 0.16436341910904062 0.8068311489795378 8 0.12307836900653686 0.6904801039992452 9 0.09551710807259345 0.6028414753464089 >10 0.8417863125222534 12.262073046978896 >50 0.038690095298156674 1.6779940962143156 >100 0.0019127172284461283 0.2052997493127283 >500 5.140803511587004E-5 0.019323030709793326 >1k 1.5422410532538273E-4 0.420131716155739 >5k 0.0 0.0 >10k+ 1.5422410532538273E-4 6.053588276718011 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141333 1.6483001281711873 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16274 0.1897959873904743 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10313 0.12027565552156577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1662528412834847E-5 0.0 0.0 0.0 0.0 2 3.498758523850454E-5 0.0 0.0 0.0 0.0 3 9.330022730267877E-5 0.0 0.0 0.0 2.3325056825669693E-5 4 9.330022730267877E-5 0.0 0.0 0.0 3.498758523850454E-5 5 1.0496275571551361E-4 0.0 0.0 0.0 5.831264206417423E-5 6 1.282878125411833E-4 0.0 0.0 0.0 6.997517047700908E-5 7 1.6327539777968785E-4 0.0 0.0 0.0 8.163769888984392E-5 8 1.749379261925227E-4 0.0 0.0 0.0 8.163769888984392E-5 9 1.8660045460535754E-4 0.0 0.0 0.0 1.51612869366853E-4 10-11 2.2158803984386206E-4 0.0 0.0 0.0 1.6910666198610529E-4 12-13 2.332505682566969E-4 0.0 0.0 0.0 1.9243171881177496E-4 14-15 2.449130966695318E-4 0.0 0.0 0.0 2.2158803984386206E-4 16-17 2.6823815349520143E-4 5.831264206417423E-6 0.0 0.0 2.2158803984386206E-4 18-19 2.857319461144537E-4 1.1662528412834847E-5 0.0 0.0 2.2158803984386206E-4 20-21 3.090570029401234E-4 1.1662528412834847E-5 0.0 0.0 2.332505682566969E-4 22-23 3.148882671465408E-4 1.1662528412834847E-5 0.0 0.0 2.332505682566969E-4 24-25 3.3821332397221057E-4 1.1662528412834847E-5 0.0 0.0 2.3908183246311435E-4 26-27 3.498758523850454E-4 1.1662528412834847E-5 0.0 5.831264206417423E-6 2.507443608759492E-4 28-29 3.965259660363848E-4 1.1662528412834847E-5 0.0 1.1662528412834847E-5 2.6823815349520143E-4 30-31 4.081884944492196E-4 1.1662528412834847E-5 0.0 1.749379261925227E-5 2.857319461144537E-4 32-33 4.256822870684719E-4 1.1662528412834847E-5 0.0 2.3325056825669693E-5 3.207195313529583E-4 34-35 4.723324007198113E-4 1.1662528412834847E-5 0.0 2.3325056825669693E-5 3.323820597657931E-4 36-37 5.423075711968203E-4 1.1662528412834847E-5 0.0 2.9156321032087114E-5 3.382133239722105E-4 38-39 5.889576848481597E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 3.498758523850454E-4 40-41 6.239452700866643E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 3.673696450042977E-4 42-43 6.705953837380036E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 3.9652596603638473E-4 44-45 7.114142331829256E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 4.373448154813067E-4 46-47 7.347392900085953E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 4.723324007198113E-4 48-49 7.930519320727696E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 5.014887217518984E-4 50-51 8.805208951690308E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 5.24813778577568E-4 52-53 9.09677216201118E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 5.423075711968203E-4 54-55 9.271710088203703E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 5.481388354032378E-4 56-57 9.796523866781271E-4 1.1662528412834847E-5 0.0 3.498758523850454E-5 5.598013638160726E-4 58-59 0.0010379650287423011 1.1662528412834847E-5 0.0 3.498758523850454E-5 6.122827416738294E-4 60-61 0.0011196027276321452 1.1662528412834847E-5 0.0 3.498758523850454E-5 6.589328553251689E-4 62-63 0.0012187342191412414 1.1662528412834847E-5 0.0 3.498758523850454E-5 6.880891763572559E-4 64-65 0.0013062031822375026 1.1662528412834847E-5 0.0 3.498758523850454E-5 7.055829689765081E-4 66-67 0.0013703470885080944 1.749379261925227E-5 0.0 3.498758523850454E-5 7.230767615957604E-4 68-69 0.0014461535231915209 2.3325056825669693E-5 0.0 3.498758523850454E-5 7.347392900085953E-4 70-71 0.0015102974294621124 2.3325056825669693E-5 0.0 3.498758523850454E-5 7.813894036599346E-4 72-73 0.0016152601851776261 2.3325056825669693E-5 0.0 3.498758523850454E-5 8.163769888984392E-4 74-75 0.0017143916766867223 2.3325056825669693E-5 0.0 3.498758523850454E-5 8.28039517311274E-4 76-77 0.0019184859239113321 3.498758523850454E-5 0.0 3.498758523850454E-5 8.397020457241089E-4 78-79 0.002099255114310272 3.498758523850454E-5 0.0 3.498758523850454E-5 8.513645741369438E-4 80-81 0.0023616620035990564 3.498758523850454E-5 0.0 3.498758523850454E-5 8.630271025497786E-4 82-83 0.0026182376286814227 4.6650113651339386E-5 0.0 3.498758523850454E-5 8.863521593754483E-4 84-85 0.003131388878846156 5.831264206417423E-5 0.0 3.498758523850454E-5 9.213397446139528E-4 86-87 0.0038369718478226643 5.831264206417423E-5 0.0 3.498758523850454E-5 9.621585940588749E-4 88-89 0.004892430669184218 5.831264206417423E-5 0.0 3.498758523850454E-5 9.679898582652922E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 520 0.0 34.694065 3 ACTCGCG 310 0.0 29.10351 50-51 CGCGTAG 350 0.0 25.09582 54-55 CGTAGCG 380 0.0 24.987488 56-57 CGTACGC 385 0.0 23.429758 20-21 CTCGCGT 380 0.0 23.116592 52-53 CGCTAGT 425 0.0 22.901367 90-91 TCGCGTA 460 0.0 19.096317 52-53 ATCAACG 6220 0.0 18.585625 10-11 ACGCTAG 490 0.0 18.410007 90-91 CAACGCA 6300 0.0 18.311884 12-13 ACGTACG 480 0.0 18.298075 20-21 AGTGGTA 7930 0.0 17.421766 5 AAGCAGT 8275 0.0 17.297188 1 GTGTACG 1090 0.0 16.986658 9 GGTATCA 8190 0.0 16.926565 8 GTATCAA 8655 0.0 16.126871 9 TCAACGC 6345 0.0 15.9373665 10-11 AACGCAG 6460 0.0 15.543369 12-13 TGGTATC 9025 0.0 15.518318 7 >>END_MODULE