FastQCFastQC Report
Sat 18 Jun 2016
SRR578569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7946851
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100114712.598034114393236No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT215940.2717302740418815No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG91350.11495119261705045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88170.1109496075867032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2237500.091.711
AGTGGTA2235550.091.603215
GGTATCA2243950.091.366178
GTATCAA2243400.091.3574149
TGGTATC2249100.091.1865167
GTGGTAT2261100.090.7854166
AGCAGTG2265250.090.64012
GCAGTGG2261100.090.634563
CAGTGGT2262700.090.420084
TCAACGC2239750.046.26787612-13
AACGCAG2240200.046.25114414-15
ATCAACG2235800.046.18111810-11
CAACGCA2236500.046.17486212-13
ACGCAGA2244900.046.03484714-15
CGCAGAG2252750.046.00454316-17
TATCAAC2253650.045.91223510-11
CAGAGTA2243350.045.72813418-19
AGAGTAC2203850.045.66048418-19
GAGTACT1247950.045.39949420-21
GCAGAGT2269700.045.3922916-17
Produced by FastQC (version 0.11.5)