FastQCFastQC Report
Sat 18 Jun 2016
SRR578569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7946851
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1045801.315992963753819No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148160.1864386283321532No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100860.12691819690591907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3500.025.7429523
CAACGCA60000.019.71312-13
ATCAACG60800.019.57057210-11
AGTGGTA74750.018.8404255
AAGCAGT79750.018.6121851
CGTAGCG2750.018.10047756-57
ACTCGCG2500.018.01428450-51
CGCGTAG2650.017.88906154-55
GGTATCA78850.017.7403838
CTCGCGT2550.017.66106252-53
CGTACGC2750.017.2412620-21
GTATCAA82800.016.8922659
TCAACGC61200.016.3829610-11
TGGTATC86400.016.2999177
ACGCAGA77150.015.51525114-15
AACGCAG63400.015.47798712-13
GTGGTAT101600.014.1880466
CGCTAGT3500.013.88784690-91
AGAGTAC84300.013.75042418-19
ACGTACG3351.8189894E-1213.4456120-21
Produced by FastQC (version 0.11.5)