##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7946851 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.992867363437416 31.0 31.0 34.0 30.0 34.0 2 32.265627353526575 34.0 31.0 34.0 30.0 34.0 3 32.33513211711155 34.0 31.0 34.0 30.0 34.0 4 35.902887823113836 37.0 35.0 37.0 35.0 37.0 5 35.782063864038726 37.0 35.0 37.0 33.0 37.0 6 35.754813195818066 37.0 35.0 37.0 33.0 37.0 7 35.7493850079736 37.0 35.0 37.0 33.0 37.0 8 35.66733766620263 37.0 35.0 37.0 33.0 37.0 9 37.30322501327885 39.0 37.0 39.0 33.0 39.0 10-11 37.29793222497817 39.0 37.0 39.0 33.5 39.0 12-13 37.2927818830377 39.0 37.0 39.0 33.0 39.0 14-15 38.60474274652941 40.0 38.0 41.0 34.0 41.0 16-17 38.558819273193876 40.0 38.0 41.0 34.0 41.0 18-19 38.49819362411602 40.0 38.0 41.0 34.0 41.0 20-21 38.431059799661526 40.0 38.0 41.0 34.0 41.0 22-23 38.31178205052542 40.0 38.0 41.0 33.0 41.0 24-25 38.22797470343914 40.0 38.0 41.0 33.0 41.0 26-27 38.06297412648104 40.0 37.0 41.0 33.0 41.0 28-29 37.96877335437647 40.0 37.0 41.0 33.0 41.0 30-31 37.871231636279575 40.0 37.0 41.0 33.0 41.0 32-33 37.73771963259409 40.0 37.0 41.0 32.5 41.0 34-35 37.607491759943656 40.0 37.0 41.0 32.0 41.0 36-37 37.48030414814623 40.0 36.5 41.0 31.5 41.0 38-39 37.3534274771227 40.0 36.0 41.0 31.0 41.0 40-41 37.20463350829152 39.5 36.0 41.0 31.0 41.0 42-43 37.04819040900603 39.0 36.0 41.0 31.0 41.0 44-45 36.89168999141924 39.0 35.0 41.0 30.0 41.0 46-47 36.977807939270534 39.0 36.0 41.0 31.0 41.0 48-49 36.95686316504487 39.0 36.0 41.0 31.0 41.0 50-51 36.93391508158389 39.0 35.5 41.0 31.0 41.0 52-53 36.947859347054575 39.0 36.0 41.0 31.0 41.0 54-55 36.820593779850654 39.0 35.0 41.0 31.0 41.0 56-57 36.67055145490963 39.0 35.0 41.0 30.0 41.0 58-59 36.45463945404286 39.0 35.0 41.0 30.0 41.0 60-61 36.23014808003825 38.5 35.0 40.5 30.0 41.0 62-63 35.96199041607802 38.0 35.0 40.0 29.0 41.0 64-65 35.65530227004382 37.5 34.0 40.0 29.0 41.0 66-67 35.340679157064855 37.0 34.0 40.0 29.0 41.0 68-69 35.01124753691745 36.5 34.0 39.5 28.5 41.0 70-71 34.6464960775029 36.0 34.0 39.0 28.0 41.0 72-73 34.22689949767524 35.5 33.5 39.0 28.0 40.5 74-75 33.84639450267785 35.0 33.0 37.5 27.0 40.0 76-77 32.40933547137099 34.5 31.5 36.0 26.0 39.0 78-79 33.01382616837789 35.0 32.5 37.0 26.0 39.0 80-81 32.961185883565705 35.0 33.0 36.0 26.0 39.0 82-83 32.68776676447061 35.0 33.0 36.0 26.0 37.5 84-85 32.40556724921608 35.0 33.0 35.5 26.0 37.0 86-87 32.12551141326294 35.0 32.5 35.0 26.0 37.0 88-89 31.874417300638957 35.0 32.0 35.0 25.0 36.0 90-91 31.633245923448168 35.0 32.0 35.0 25.0 36.0 92-93 31.39609500668881 35.0 32.0 35.0 24.5 36.0 94-95 31.141877141021016 35.0 32.0 35.0 24.0 35.5 96-97 30.543268207746692 34.0 31.5 35.0 19.0 35.0 98-99 30.517779117791434 34.0 31.5 35.0 19.5 35.0 100-101 29.932153692072497 34.0 30.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4216574275517502 1101 2 0.40039354290443185 1101 3 0.3812123029438368 1101 4 0.2896836140203618 1101 5 0.27227185430461986 1101 6 0.30012871818232867 1101 7 0.3076630641418845 1101 8 0.38641212541601533 1101 9 0.4564801735817028 1101 10-11 0.4035817186289492 1101 12-13 0.4171542856999224 1101 14-15 0.5672396040735563 1101 16-17 0.5472206559043471 1101 18-19 0.5352959504034018 1101 20-21 0.6085117433081777 1101 22-23 0.6326293188369405 1101 24-25 0.6725126294025472 1101 26-27 0.6807525104288317 1101 28-29 0.6878529326964298 1101 30-31 0.6898894836253646 1101 32-33 0.6371761324615619 1101 34-35 0.6633521953021742 1101 36-37 0.6322084674800053 1101 38-39 0.6731437976390282 1101 40-41 0.6785559582103033 1101 42-43 0.6942569183885965 1101 44-45 0.7259344569540573 1101 46-47 0.7535004911018603 1101 48-49 0.6805964350485993 1101 50-51 0.6532186730677907 1101 52-53 0.610387532582763 1101 54-55 0.7180259281890429 1101 56-57 0.7195159232489488 1101 58-59 0.7499923367539836 1101 60-61 0.6207610328912097 1101 62-63 0.7097644575706639 1101 64-65 0.6290584307850224 1101 66-67 0.616141351814818 1101 68-69 0.6291175320630984 1101 70-71 0.6117392178124064 1101 72-73 0.5949483736972851 1101 74-75 0.6214246550249882 1101 76-77 0.619999841112346 1101 78-79 0.5132117941438423 1101 80-81 0.4610284503288966 1101 82-83 0.41377453356441407 1101 84-85 0.38972864844760835 1101 86-87 0.3622919562627942 1101 88-89 0.38006935054451674 1101 90-91 0.39908348467369237 1101 92-93 0.4522520258606306 1101 94-95 0.43926506596317694 1101 96-97 0.7875463057724339 1101 98-99 0.5376650803028689 1101 100-101 0.45967304149368005 1102 1 0.4111999053305695 1102 2 0.388323031560752 1102 3 0.3610457544868524 1102 4 0.25933885061667183 1102 5 0.28419262028403836 1102 6 0.29999566855110515 1102 7 0.27252071175985293 1102 8 0.31471632902512425 1102 9 0.47140681784835436 1102 10-11 0.39285623366984623 1102 12-13 0.403358214946266 1102 14-15 0.4850092806947899 1102 16-17 0.512058199811591 1102 18-19 0.5487415282797556 1102 20-21 0.5851854119559832 1102 22-23 0.588133662579402 1102 24-25 0.6418620881390922 1102 26-27 0.6712456985634532 1102 28-29 0.6598152421756254 1102 30-31 0.716435346604662 1102 32-33 0.7303411243163467 1102 34-35 0.7585281688949763 1102 36-37 0.7788394479756207 1102 38-39 0.8156738037178641 1102 40-41 0.7677637738396825 1102 42-43 0.8033697453698423 1102 44-45 0.852808100234256 1102 46-47 0.890502352365516 1102 48-49 0.8850602554223883 1102 50-51 0.885127699487434 1102 52-53 0.8074779414167352 1102 54-55 0.8415850298731513 1102 56-57 0.7657814321338208 1102 58-59 0.7957410228851955 1102 60-61 0.8153008894021738 1102 62-63 0.7925963078971918 1102 64-65 0.7293333685452552 1102 66-67 0.7838041188750324 1102 68-69 0.8300787341216349 1102 70-71 0.8303444143043279 1102 72-73 0.8114086490523889 1102 74-75 0.7188111323399298 1102 76-77 0.6892073269401422 1102 78-79 0.7334982967000627 1102 80-81 0.7213820281814662 1102 82-83 0.7133267169027349 1102 84-85 0.726857044732931 1102 86-87 0.6868486859146969 1102 88-89 0.6112557031249679 1102 90-91 0.5924036146396894 1102 92-93 0.6147703100071595 1102 94-95 0.6378947181365362 1102 96-97 1.0254128318312006 1102 98-99 0.7962874264771749 1102 100-101 0.7602934537581696 1103 1 0.3303056597199472 1103 2 0.2775543317157698 1103 3 0.24988695824105633 1103 4 0.22783003334662766 1103 5 0.16080301856555224 1103 6 0.24220469087146768 1103 7 0.25443560110352337 1103 8 0.06574277160622444 1103 9 0.1631581071396795 1103 10-11 0.3667260181010974 1103 12-13 0.314463374544971 1103 14-15 0.42156909877944315 1103 16-17 0.4397945273944046 1103 18-19 0.3769795724112228 1103 20-21 0.38898180124643744 1103 22-23 0.4219551334022995 1103 24-25 0.2359250927268448 1103 26-27 0.21458016757023302 1103 28-29 0.38720982074486443 1103 30-31 0.25804591776438457 1103 32-33 0.4737286054341965 1103 34-35 0.47099167441290746 1103 36-37 0.5169573514620751 1103 38-39 0.5445390567149531 1103 40-41 0.43727674646115844 1103 42-43 0.5450136920663127 1103 44-45 0.6153854049290217 1103 46-47 0.47644774361670983 1103 48-49 0.4510550282592547 1103 50-51 0.4579248003588745 1103 52-53 0.5273919665851494 1103 54-55 0.6427456821320945 1103 56-57 0.5751348101159408 1103 58-59 0.2864855899945056 1103 60-61 0.28558846595409904 1103 62-63 0.14783982888999248 1103 64-65 0.3917123948812318 1103 66-67 0.35810432232521805 1103 68-69 0.31192160471059793 1103 70-71 0.3478145312725829 1103 72-73 0.5006685002553013 1103 74-75 0.44127780842595854 1103 76-77 0.27361446938136424 1103 78-79 0.37808528783641293 1103 80-81 0.39890296138384684 1103 82-83 0.4192347272041772 1103 84-85 0.42328930202072 1103 86-87 0.3284769034329642 1103 88-89 0.33428880433064023 1103 90-91 0.46840226848031463 1103 92-93 0.40389925099126955 1103 94-95 0.4214313436840307 1103 96-97 0.5921917936702492 1103 98-99 0.37011807636114114 1103 100-101 0.5706407133787792 1104 1 0.2848585036796223 1104 2 0.2695185293467972 1104 3 0.23294418542104012 1104 4 0.14404491249509022 1104 5 0.18833236132024922 1104 6 0.2008557276064309 1104 7 0.18108133216423994 1104 8 0.25676325348187845 1104 9 0.23132777926186776 1104 10-11 0.29372387226130314 1104 12-13 0.2832471507685881 1104 14-15 0.34152661894244574 1104 16-17 0.36493854598494124 1104 18-19 0.3912963158755147 1104 20-21 0.33456551020409364 1104 22-23 0.4403457026855264 1104 24-25 0.4197111240464153 1104 26-27 0.5118379160647848 1104 28-29 0.5125569634360474 1104 30-31 0.5301305834872778 1104 32-33 0.5356116810222105 1104 34-35 0.5216277820405679 1104 36-37 0.5606459911852966 1104 38-39 0.5283496509143788 1104 40-41 0.5337972817465513 1104 42-43 0.5653991741876254 1104 44-45 0.5513269075720473 1104 46-47 0.39558086625928723 1104 48-49 0.5189774786754668 1104 50-51 0.3806294808165305 1104 52-53 0.44530427590219546 1104 54-55 0.49948540197663505 1104 56-57 0.5315199874527181 1104 58-59 0.5553301379012012 1104 60-61 0.49193920438484184 1104 62-63 0.2740019721824538 1104 64-65 0.4807235981162705 1104 66-67 0.5194036866505911 1104 68-69 0.587131237916779 1104 70-71 0.5246487975420351 1104 72-73 0.6354291456187511 1104 74-75 0.5454188047236315 1104 76-77 0.43025188340716625 1104 78-79 0.4095720753811918 1104 80-81 0.5452011424300736 1104 82-83 0.5975864168682037 1104 84-85 0.5876780877086034 1104 86-87 0.5978658758244109 1104 88-89 0.552371874149614 1104 90-91 0.5445110860059224 1104 92-93 0.5716988631351825 1104 94-95 0.6076790303049471 1104 96-97 0.9562638352681994 1104 98-99 0.702346226906517 1104 100-101 0.7382509629899303 1105 1 0.135195023936447 1105 2 0.14524739474518356 1105 3 0.09669536885790819 1105 4 0.07759315667276923 1105 5 0.11010021163125572 1105 6 0.0787057056483107 1105 7 0.10833947721693704 1105 8 0.19144809004865238 1105 9 0.20139280454938557 1105 10-11 0.17340752304406237 1105 12-13 0.16595733259840983 1105 14-15 0.15619305717635967 1105 16-17 0.15541279769595917 1105 18-19 0.09131056645634317 1105 20-21 0.18283977042897703 1105 22-23 0.16225700384662645 1105 24-25 0.1565919602830519 1105 26-27 0.12520396793425448 1105 28-29 0.19850597882654597 1105 30-31 0.13856163958753598 1105 32-33 0.20809744819471376 1105 34-35 0.12397761024735132 1105 36-37 0.17348808191473353 1105 38-39 0.13982121084816157 1105 40-41 0.2337735239527703 1105 42-43 0.09743069663933568 1105 44-45 0.2123441910260624 1105 46-47 0.22987762254287958 1105 48-49 0.22269926799051376 1105 50-51 0.160615076695386 1105 52-53 0.20283209963580617 1105 54-55 0.29482089292610425 1105 56-57 0.28067755573552233 1105 58-59 0.2951752525865743 1105 60-61 0.25002580241828554 1105 62-63 0.28097374933288677 1105 64-65 0.2692448549377602 1105 66-67 0.28216936383689273 1105 68-69 0.2865521096735364 1105 70-71 0.28951272246093396 1105 72-73 0.2920858050425963 1105 74-75 0.35033060756050105 1105 76-77 0.354280952861167 1105 78-79 0.32050967744115866 1105 80-81 0.272119329762063 1105 82-83 0.2790540372218757 1105 84-85 0.33120702042517536 1105 86-87 0.1967923191896901 1105 88-89 0.19188273652387977 1105 90-91 0.22398041244204236 1105 92-93 0.26174273756875266 1105 94-95 0.32847175900673875 1105 96-97 0.6683820039095352 1105 98-99 0.4499018199546576 1105 100-101 0.4127427957542551 1106 1 -0.04484858493412602 1106 2 -0.10085604111223034 1106 3 -0.07403854909004792 1106 4 -0.05645200998773703 1106 5 -0.0293076052886434 1106 6 -0.0716306428880884 1106 7 -0.02579930659376828 1106 8 -0.022191319020819833 1106 9 0.015246938532776255 1106 10-11 -0.07907431829634959 1106 12-13 -0.07444854852267468 1106 14-15 -0.20630477026463723 1106 16-17 -0.13194326433973202 1106 18-19 -0.11344693317246168 1106 20-21 -0.15013696993095493 1106 22-23 -0.13094345669274787 1106 24-25 -0.02810178230880922 1106 26-27 -0.04798245296557013 1106 28-29 -0.10184428236878773 1106 30-31 -0.22039685158736688 1106 32-33 -0.301900919345222 1106 34-35 -0.31831087066800023 1106 36-37 -0.2362214375012428 1106 38-39 -0.27900011848638684 1106 40-41 -0.2108026870360149 1106 42-43 -0.18964446046208394 1106 44-45 -0.19877017396336782 1106 46-47 -0.20899976217813077 1106 48-49 -0.21122920934867295 1106 50-51 -0.23306637814086884 1106 52-53 -0.1141074252586165 1106 54-55 -0.16591021833613695 1106 56-57 -0.1681647778493982 1106 58-59 -0.1306210979007716 1106 60-61 -0.25847937140351007 1106 62-63 -0.1974456773765425 1106 64-65 -0.08862348865978475 1106 66-67 -0.14178675801080942 1106 68-69 -0.14437868654565733 1106 70-71 -0.11378566290805736 1106 72-73 -0.10838745482347889 1106 74-75 -0.10443314620875555 1106 76-77 -0.15970214063580812 1106 78-79 -0.10815976133660143 1106 80-81 -0.15126566742969771 1106 82-83 -0.19378988106733175 1106 84-85 -0.13628441295515614 1106 86-87 -0.19871810165754766 1106 88-89 -0.23061185049599686 1106 90-91 -0.1799284080905572 1106 92-93 -0.1371359217892092 1106 94-95 -0.0982377684490423 1106 96-97 0.2123835327147603 1106 98-99 -0.015506016771457354 1106 100-101 -7.779433723129614E-4 1107 1 -0.21791326114668053 1107 2 -0.26368678962304415 1107 3 -0.21259592422624962 1107 4 -0.15410149054968514 1107 5 -0.17542291265004906 1107 6 -0.14698439695845167 1107 7 -0.16769576345414805 1107 8 -0.19454927055878812 1107 9 -0.2672251659324303 1107 10-11 -0.253845326431275 1107 12-13 -0.2240353879731387 1107 14-15 -0.28246504221274193 1107 16-17 -0.346175464976362 1107 18-19 -0.3147747776991494 1107 20-21 -0.32386414319913825 1107 22-23 -0.21986288554466427 1107 24-25 -0.1649181173357448 1107 26-27 -0.18848667775894512 1107 28-29 -0.22051141410435093 1107 30-31 -0.3040150236958965 1107 32-33 -0.33724620115290094 1107 34-35 -0.31031915298791546 1107 36-37 -0.350092536683853 1107 38-39 -0.3886836994438525 1107 40-41 -0.3162890328773287 1107 42-43 -0.39981084446521464 1107 44-45 -0.2853508672175238 1107 46-47 -0.31211545732342216 1107 48-49 -0.38916194285693706 1107 50-51 -0.3697745693621428 1107 52-53 -0.3282646902254669 1107 54-55 -0.3057203144247538 1107 56-57 -0.321745472887045 1107 58-59 -0.31616487372091484 1107 60-61 -0.3909809800569519 1107 62-63 -0.4879170730278304 1107 64-65 -0.46843495804352386 1107 66-67 -0.33534155790017905 1107 68-69 -0.34709695734543544 1107 70-71 -0.35254539206062674 1107 72-73 -0.3281225177867739 1107 74-75 -0.3071791440662679 1107 76-77 -0.37456288812874305 1107 78-79 -0.19416786505172468 1107 80-81 -0.23737819189391018 1107 82-83 -0.22890023131746062 1107 84-85 -0.2515604866763894 1107 86-87 -0.2438630575433116 1107 88-89 -0.24226467996165724 1107 90-91 -0.22236880701877482 1107 92-93 -0.2097793288961789 1107 94-95 -0.18464585710632164 1107 96-97 0.0724151064001326 1107 98-99 -0.15730762964160405 1107 100-101 -0.27236945801364243 1108 1 -0.348894804018272 1108 2 -0.34347749649130677 1108 3 -0.3670266811034075 1108 4 -0.2452101865779639 1108 5 -0.23026090306115066 1108 6 -0.2457796524915281 1108 7 -0.2563263363751034 1108 8 -0.22519755826930776 1108 9 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-0.16951541856283114 2305 16-17 -0.15619430400270318 2305 18-19 -0.2043070249948542 2305 20-21 -0.16297555732217717 2305 22-23 -0.17723059121634321 2305 24-25 -0.1210689629066195 2305 26-27 -0.19472291862972213 2305 28-29 -0.1642408067702732 2305 30-31 -0.2365311383401547 2305 32-33 -0.225819750707565 2305 34-35 -0.23921056469336577 2305 36-37 -0.1861662705128495 2305 38-39 -0.2361944447898594 2305 40-41 -0.2196081078512364 2305 42-43 -0.25566402139806854 2305 44-45 -0.20121903722996848 2305 46-47 -0.0936973851330194 2305 48-49 -0.12124014297626928 2305 50-51 -0.18702028618727695 2305 52-53 -0.23994952716521567 2305 54-55 -0.14299947239368294 2305 56-57 -0.16258410551900226 2305 58-59 -0.09306640530935795 2305 60-61 -0.10442389349763204 2305 62-63 -0.13540780034832522 2305 64-65 -0.11138435450240536 2305 66-67 -0.17720852919979535 2305 68-69 -0.13187324320717408 2305 70-71 -0.1207493214262243 2305 72-73 -0.1557441214019022 2305 74-75 -0.18071896415847988 2305 76-77 -0.19482903579466182 2305 78-79 -0.16428999729350835 2305 80-81 -0.14282152843966855 2305 82-83 -0.06697442416403021 2305 84-85 -0.06302773217417013 2305 86-87 -0.0754174600902573 2305 88-89 -0.11609133509286096 2305 90-91 -0.13173946011198368 2305 92-93 -0.09858550092247498 2305 94-95 -0.20431205490708138 2305 96-97 0.06681107804852715 2305 98-99 -0.1810564463762283 2305 100-101 -0.0965110083132501 2306 1 -0.2524120691030447 2306 2 -0.2728254782654176 2306 3 -0.2541080802948059 2306 4 -0.19876738209210743 2306 5 -0.21082886656128608 2306 6 -0.1663959623436213 2306 7 -0.21553719603156196 2306 8 -0.18552099420212187 2306 9 -0.3624446896589504 2306 10-11 -0.29909856476477614 2306 12-13 -0.3050351088241996 2306 14-15 -0.3713286885337652 2306 16-17 -0.4180535719148395 2306 18-19 -0.4148598190728876 2306 20-21 -0.4782848418734815 2306 22-23 -0.45503776438282983 2306 24-25 -0.47106664745402327 2306 26-27 -0.516969807158155 2306 28-29 -0.5665247546522565 2306 30-31 -0.5925595734767555 2306 32-33 -0.685815035469389 2306 34-35 -0.6302645996903493 2306 36-37 -0.6119504999407326 2306 38-39 -0.7748197648992488 2306 40-41 -0.6123920996907444 2306 42-43 -0.6997528699915208 2306 44-45 -0.7196750579395186 2306 46-47 -0.6352061894514662 2306 48-49 -0.6197480112694578 2306 50-51 -0.6670059591149524 2306 52-53 -0.6190943983082207 2306 54-55 -0.5798884262893367 2306 56-57 -0.6371789116225273 2306 58-59 -0.6459862258245437 2306 60-61 -0.5928347762176074 2306 62-63 -0.5560464437291515 2306 64-65 -0.6226254113888743 2306 66-67 -0.551671930791386 2306 68-69 -0.5910660551712752 2306 70-71 -0.6267280661383268 2306 72-73 -0.6218150742139912 2306 74-75 -0.5726746823967304 2306 76-77 -0.5949436738914144 2306 78-79 -0.5291122559622323 2306 80-81 -0.5510446202873567 2306 82-83 -0.5471226249201351 2306 84-85 -0.46803847660867604 2306 86-87 -0.4727795845841136 2306 88-89 -0.4689508536588569 2306 90-91 -0.5165387306013933 2306 92-93 -0.5513239485051784 2306 94-95 -0.5652383133211032 2306 96-97 -0.30274215794981174 2306 98-99 -0.5755120561541496 2306 100-101 -0.5282305741595721 2307 1 -0.4501251995846296 2307 2 -0.4756027567795371 2307 3 -0.4799310905642429 2307 4 -0.3938792365193464 2307 5 -0.3821298598656213 2307 6 -0.3774531575293878 2307 7 -0.44637061048845084 2307 8 -0.4266334637422844 2307 9 -0.5036108730965267 2307 10-11 -0.588340717937264 2307 12-13 -0.5588090730112825 2307 14-15 -0.8598758696463236 2307 16-17 -0.7394341930192567 2307 18-19 -0.7051580233690018 2307 20-21 -0.6236546682850488 2307 22-23 -0.6152483896500982 2307 24-25 -0.6032040635504288 2307 26-27 -0.6844184069933164 2307 28-29 -0.6771024011511244 2307 30-31 -0.7044305666617845 2307 32-33 -0.7449846375374563 2307 34-35 -0.7338041249559666 2307 36-37 -0.813028061742699 2307 38-39 -0.760034309644297 2307 40-41 -0.6815015826658524 2307 42-43 -0.8075431859489797 2307 44-45 -0.7200381520536254 2307 46-47 -0.7289470447163566 2307 48-49 -0.6201325149121217 2307 50-51 -0.7169327061960402 2307 52-53 -0.8193874762965478 2307 54-55 -0.8456959894280729 2307 56-57 -0.8755186562224395 2307 58-59 -0.8473687542288744 2307 60-61 -0.9501342452710801 2307 62-63 -0.9203271120506642 2307 64-65 -0.9179406923967903 2307 66-67 -0.8373939568434707 2307 68-69 -0.8431866405545989 2307 70-71 -0.938449623808836 2307 72-73 -1.2693168370927808 2307 74-75 -0.9534831660290806 2307 76-77 -0.8654510899858678 2307 78-79 -0.9149013596013162 2307 80-81 -0.8115885319577245 2307 82-83 -0.7879391793885695 2307 84-85 -0.8351806830488968 2307 86-87 -0.8088127727392873 2307 88-89 -0.8419000707949671 2307 90-91 -0.9092210622262975 2307 92-93 -0.8389276065605742 2307 94-95 -0.8816842076959368 2307 96-97 -0.7176257520267164 2307 98-99 -1.0482037349359707 2307 100-101 -1.0545202589790428 2308 1 -1.458047420081698 2308 2 -0.9089353741862958 2308 3 -0.8979362909508914 2308 4 -0.6020876486645932 2308 5 -0.6212392008116154 2308 6 -0.6661800407002474 2308 7 -0.6449390283115193 2308 8 -0.6484192243946723 2308 9 -0.9332075506612796 2308 10-11 -0.8835774854543814 2308 12-13 -0.794333708104709 2308 14-15 -1.0405035609703006 2308 16-17 -0.9642958892037043 2308 18-19 -0.9902716886114931 2308 20-21 -0.9818362196851425 2308 22-23 -1.0426823040278421 2308 24-25 -1.2101765424529916 2308 26-27 -1.2284474038794002 2308 28-29 -1.2480590813340058 2308 30-31 -1.1829090909043458 2308 32-33 -1.2032295109432383 2308 34-35 -1.2903553375636037 2308 36-37 -1.2939604183202107 2308 38-39 -1.3222302638272367 2308 40-41 -1.2675412109415731 2308 42-43 -1.1680092070538635 2308 44-45 -1.1944028472104975 2308 46-47 -1.134714173739603 2308 48-49 -1.2383738120846814 2308 50-51 -1.283125235990184 2308 52-53 -1.2414072504485105 2308 54-55 -1.2095313654719035 2308 56-57 -1.1366838786172337 2308 58-59 -1.0978660207292208 2308 60-61 -1.0452883412669962 2308 62-63 -1.0944019919309795 2308 64-65 -1.0685850203145222 2308 66-67 -1.0656861446966843 2308 68-69 -1.1260992320392234 2308 70-71 -1.0826688551844654 2308 72-73 -1.1185661460836585 2308 74-75 -1.1924115550334946 2308 76-77 -1.1517779193062516 2308 78-79 -1.5570481680246502 2308 80-81 -1.23097019517434 2308 82-83 -1.159600358318059 2308 84-85 -1.133253016250407 2308 86-87 -1.1280308447338285 2308 88-89 -1.1808007681394486 2308 90-91 -1.165165194096133 2308 92-93 -1.077625179737474 2308 94-95 -1.2332232283539781 2308 96-97 -1.0671390204459037 2308 98-99 -1.3154536037894253 2308 100-101 -1.3800809914703187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 67.0 4 156.0 5 423.0 6 878.0 7 1635.0 8 3071.0 9 4860.0 10 6543.0 11 8162.0 12 9692.0 13 11340.0 14 12724.0 15 14181.0 16 16367.0 17 19055.0 18 21968.0 19 25354.0 20 27136.0 21 28409.0 22 30975.0 23 36071.0 24 44426.0 25 55731.0 26 71310.0 27 91739.0 28 117542.0 29 146316.0 30 181291.0 31 224714.0 32 276589.0 33 348242.0 34 460989.0 35 685815.0 36 936076.0 37 1579488.0 38 1888297.0 39 556934.0 40 2247.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.536769781593154 30.42943500507623 29.244490746689568 22.789304466641052 2 22.528623025109123 29.190444983593732 27.569452109568772 20.711479881728373 3 21.631381943329334 28.097426206872946 28.066103862829024 22.205087986968696 4 21.935991114917535 28.037765627259166 28.11124171569372 21.915001542129584 5 22.512069659356627 28.120601209092584 28.344830224445456 21.022498907105337 6 22.489486716184814 28.177676918819795 27.381487333787934 21.951349031207457 7 22.653539118828327 28.29858015457947 27.828972759146986 21.21890796744522 8 21.86086161832909 28.47510260522187 27.856406928918393 21.807628847530648 9 21.54352534785204 28.159097827970598 28.407147816524557 21.8902290076528 10-11 21.570783194500564 28.548383504359148 27.881031115343674 21.999802185796614 12-13 21.58712929184151 28.652613469159043 27.713040045673438 22.04721719332601 14-15 21.731368815144513 28.697096497719667 27.547181896325977 22.024352790809846 16-17 21.92550860711998 28.48039430964542 27.528124033028934 22.065973050205674 18-19 21.891916684986292 28.514577661013146 27.52866512786008 22.06484052614048 20-21 21.992107313953664 28.4239820275981 27.573865421661992 22.010045236786244 22-23 21.926614249621785 28.428737577220137 27.592204073504668 22.052444099653414 24-25 21.923112812301778 28.37346262527826 27.525783334605386 22.177641227814572 26-27 22.080765039150492 28.28065656044479 27.646867834679462 21.99171056572526 28-29 22.125581566836978 28.316599870816756 27.571078154101542 21.986740408244724 30-31 22.09239231992647 28.336437917358715 27.521838524467114 22.049331238247703 32-33 22.104057317797956 28.389666548422767 27.432866175545506 22.073409958233768 34-35 22.112167448464806 28.38563979619097 27.45807112779641 22.044121627547817 36-37 22.143777453484407 28.39452381830237 27.395417379789805 22.06628134842342 38-39 22.120346788935645 28.38331183005696 27.402143314376975 22.09419806663042 40-41 22.122039283233068 28.42827932724547 27.400337567673034 22.049343821848428 42-43 22.16100440287606 28.432847174308414 27.33792290808019 22.068225514735335 44-45 22.16603155136544 28.441385147399895 27.33737552144869 22.05520777978598 46-47 22.146169731014822 28.460042126122797 27.31856475719532 22.075223385667066 48-49 22.149307946002764 28.46946545241631 27.337702695067517 22.04352390651341 50-51 22.22911314179667 28.440693049360057 27.297038789326738 22.033155019516535 52-53 22.19447678080286 28.52459420718974 27.224639042559122 22.056289969448276 54-55 22.20635569988666 28.541129058541554 27.1604249280627 22.092090313509086 56-57 22.209105216644932 28.54983061844245 27.164546057299933 22.07651810761269 58-59 22.208960505236604 28.629000342399774 27.139982868685976 22.02205628367765 60-61 22.205852346588046 28.60301882010682 27.10878459776466 22.082344235540475 62-63 22.21506564210054 28.62102418400463 27.114910467281756 22.048999706613074 64-65 22.19325190044332 28.675188723050578 27.134316450710532 21.997242925795568 66-67 22.193140766966877 28.700000855686465 27.10434064576141 22.00251773158525 68-69 22.191881680612376 28.750082029569153 27.08275042266856 21.975285867149914 70-71 22.209486207290116 28.778075771724698 27.04828194038815 21.964156080597036 72-73 22.147950986353333 28.782203963131042 27.049089148301313 22.02075590221431 74-75 22.22961509136894 28.76543363676185 27.04105162714219 21.96389964472702 76-77 22.19799596320011 28.864624832114988 26.99828524199381 21.9390939626911 78-79 22.20963235935681 28.847710475486366 26.978918488321685 21.96373867683514 80-81 22.15968448363036 28.877009822513234 26.987763447284618 21.975542246571784 82-83 22.193408673754064 28.928656549409403 26.93415846555407 21.943776311282466 84-85 22.187913195169166 28.924827510828138 26.94783974258497 21.93941955141772 86-87 22.16120933266664 28.987068870488013 26.915458912549497 21.93626288429585 88-89 22.17406046670919 29.039516230037698 26.878486968920413 21.907936334332703 90-91 22.158163698980445 29.003859158818436 26.88684461421234 21.95113252798878 92-93 22.158606746092655 29.068093027757257 26.867080304830054 21.906219921320034 94-95 22.136526062915017 29.10524555103251 26.865359615121882 21.892868770930587 96-97 22.145010245251683 29.124532225220772 26.831120598446002 21.899336931081542 98-99 22.111711260631125 29.173437264569095 26.807179017620374 21.907672457179398 100-101 22.169115073087024 29.193739665040276 26.743337398060717 21.89380786381199 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 116540.0 1 65067.0 2 10176.5 3 6202.5 4 5432.0 5 4738.0 6 4269.5 7 4315.5 8 4328.0 9 4144.5 10 3872.5 11 4171.5 12 7763.5 13 7697.0 14 4436.0 15 4732.5 16 5394.0 17 6588.5 18 8881.0 19 10869.0 20 13156.5 21 16761.0 22 21431.0 23 26728.5 24 32736.0 25 40287.5 26 52141.5 27 65294.0 28 73799.5 29 81353.0 30 92770.0 31 108645.5 32 125679.0 33 143671.5 34 162301.5 35 177676.0 36 191658.0 37 204790.5 38 216228.5 39 225638.0 40 236385.0 41 246464.0 42 251487.0 43 258470.5 44 268904.5 45 279924.5 46 288003.0 47 290779.5 48 293541.5 49 299495.0 50 301503.5 51 297258.5 52 289995.0 53 278230.5 54 260286.5 55 241892.5 56 222834.0 57 201616.5 58 184902.0 59 163852.0 60 134738.0 61 104097.0 62 76533.5 63 55788.0 64 40298.5 65 27797.5 66 18632.5 67 13200.0 68 8183.5 69 4104.5 70 1945.5 71 794.5 72 355.5 73 180.0 74 93.0 75 53.5 76 42.0 77 28.0 78 17.5 79 13.0 80 9.5 81 7.0 82 5.5 83 3.5 84 1.5 85 3.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012482931918567492 2 0.017768044222799696 3 0.005624869523789989 4 6.040128347693948E-4 5 8.179340470835555E-4 6 0.0 7 0.0 8 0.0077766652476559586 9 0.010972899831644005 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0696060615708033E-4 24-25 0.004913896082863515 26-27 0.007436908028098174 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.291800362181196E-6 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 5.662620325963076E-5 62-63 9.437700543271794E-5 64-65 2.6425561521161025E-4 66-67 1.8875401086543587E-4 68-69 2.7054741557379147E-4 70-71 1.0066880579489914E-4 72-73 3.7750802173087175E-5 74-75 5.033440289744957E-5 76-77 0.12421272275018116 78-79 0.05529234158284835 80-81 6.291800362181197E-5 82-83 0.0020762941195197945 84-85 0.009720831559569949 86-87 0.11556778905254421 88-89 0.1323417288181193 90-91 0.1456237193826838 92-93 0.14361663506714797 94-95 0.11145924341603988 96-97 1.2128703558176692 98-99 0.07499196851683768 100-101 0.06743551628185807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7946851.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.35193349997577 #Duplication Level Percentage of deduplicated Percentage of total 1 91.14656730443784 65.03483809057315 2 5.223618289775352 7.4543052968261625 3 1.2821678701378663 2.744554698176478 4 0.5457954680502621 1.5577424776364182 5 0.32922130168197494 1.1745288212193867 6 0.2194571933022534 0.9395217037556228 7 0.16046421046979695 0.8014602172196948 8 0.12679318805914808 0.7237551298117004 9 0.09319120777307174 0.5984435573825976 >10 0.8420329569009406 12.195156500376676 >50 0.028999122836954428 1.2837760846771629 >100 0.0012706848978204029 0.13400737800912846 >500 1.0530041918110721E-4 0.05263128648889205 >1k 1.5795062874839934E-4 0.44103590529183545 >5k 0.0 0.0 >10k+ 1.5795062874839934E-4 4.864242852555148 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104580 1.315992963753819 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14816 0.1864386283321532 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10086 0.12691819690591907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2583600724362393E-5 0.0 0.0 0.0 2.5167201448724785E-5 2 2.5167201448724785E-5 0.0 0.0 0.0 5.033440289744957E-5 3 3.7750802173087175E-5 0.0 0.0 0.0 5.033440289744957E-5 4 5.033440289744957E-5 0.0 0.0 0.0 8.808520507053675E-5 5 6.291800362181197E-5 0.0 0.0 0.0 1.0066880579489914E-4 6 6.291800362181197E-5 0.0 0.0 1.2583600724362393E-5 1.2583600724362393E-4 7 8.808520507053675E-5 0.0 0.0 1.2583600724362393E-5 1.3841960796798632E-4 8 1.0066880579489914E-4 0.0 0.0 1.2583600724362393E-5 1.635868094167111E-4 9 1.510032086923487E-4 0.0 0.0 1.2583600724362393E-5 1.635868094167111E-4 10-11 1.887540108654359E-4 0.0 0.0 1.2583600724362393E-5 1.698786097788923E-4 12-13 2.1392121231416066E-4 0.0 0.0 1.2583600724362393E-5 1.887540108654359E-4 14-15 2.2650481303852305E-4 0.0 0.0 1.2583600724362393E-5 2.1392121231416066E-4 16-17 2.5167201448724787E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 2.2650481303852305E-4 18-19 2.7683921593597263E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 2.3279661340070427E-4 20-21 2.89422816660335E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 2.579638148494291E-4 22-23 3.082982177468786E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 2.89422816660335E-4 24-25 3.397572195577846E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 3.145900181090598E-4 26-27 3.837998220930529E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 3.20881818471241E-4 28-29 4.1525882390395895E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 3.397572195577846E-4 30-31 4.404260253526837E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 3.52340820282147E-4 32-33 4.6559322680140853E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 3.7750802173087175E-4 34-35 4.844686278879521E-4 1.2583600724362393E-5 0.0 1.2583600724362393E-5 4.1525882390395895E-4 36-37 5.410948311475828E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 4.3413422499050255E-4 38-39 5.47386631509764E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 4.404260253526837E-4 40-41 5.914292340450325E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 4.404260253526837E-4 42-43 6.41763636942482E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 4.781768275257709E-4 44-45 6.543472376668444E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 5.285112304232204E-4 46-47 7.361406423751999E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 5.7884563332067E-4 48-49 7.801832449104683E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 5.977210344072137E-4 50-51 8.179340470835555E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 6.41763636942482E-4 52-53 9.123110525162734E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 6.858062394777503E-4 54-55 9.87812656862448E-4 1.2583600724362393E-5 0.0 2.5167201448724785E-5 7.109734409264752E-4 56-57 0.0010444388601220786 1.2583600724362393E-5 0.0 2.5167201448724785E-5 7.801832449104683E-4 58-59 0.001088481462657347 1.2583600724362393E-5 0.0 2.5167201448724785E-5 8.116422467213742E-4 60-61 0.0012269010706253331 2.5167201448724785E-5 0.0 2.5167201448724785E-5 8.305176478079179E-4 62-63 0.001409363281128588 2.5167201448724785E-5 0.0 2.5167201448724785E-5 8.368094481700991E-4 64-65 0.0014534058836638563 2.5167201448724785E-5 0.0 2.5167201448724785E-5 8.745602503431862E-4 66-67 0.0015603664898209365 2.5167201448724785E-5 0.0 2.5167201448724785E-5 9.248946532406358E-4 68-69 0.001698786097788923 2.5167201448724785E-5 0.0 2.5167201448724785E-5 9.62645455413723E-4 70-71 0.0018812483082921777 2.5167201448724785E-5 0.0 2.5167201448724785E-5 9.87812656862448E-4 72-73 0.0020070843155358016 2.5167201448724785E-5 0.0 2.5167201448724785E-5 0.0010129798583111726 74-75 0.0021643793245903313 2.5167201448724785E-5 0.0 2.5167201448724785E-5 0.0010444388601220786 76-77 0.00231538253328268 3.145900181090598E-5 0.0 2.5167201448724785E-5 0.0010696060615708034 78-79 0.002504136544148116 3.7750802173087175E-5 0.0 2.5167201448724785E-5 0.001088481462657347 80-81 0.0027306413571866394 4.404260253526837E-5 0.0 2.5167201448724785E-5 0.0011136486641060719 82-83 0.0030703985767444237 5.033440289744957E-5 0.0 2.5167201448724785E-5 0.001126232264830434 84-85 0.0034919492010105635 5.033440289744957E-5 0.0 2.5167201448724785E-5 0.0011702748673657025 86-87 0.004058211233606872 5.033440289744957E-5 0.0 2.5167201448724785E-5 0.0011765666677278838 88-89 0.004939063284312239 5.662620325963077E-5 0.0 2.5167201448724785E-5 0.0011954420688144271 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 350 0.0 25.742952 3 CAACGCA 6000 0.0 19.713 12-13 ATCAACG 6080 0.0 19.570572 10-11 AGTGGTA 7475 0.0 18.840425 5 AAGCAGT 7975 0.0 18.612185 1 CGTAGCG 275 0.0 18.100477 56-57 ACTCGCG 250 0.0 18.014284 50-51 CGCGTAG 265 0.0 17.889061 54-55 GGTATCA 7885 0.0 17.740383 8 CTCGCGT 255 0.0 17.661062 52-53 CGTACGC 275 0.0 17.24126 20-21 GTATCAA 8280 0.0 16.892265 9 TCAACGC 6120 0.0 16.38296 10-11 TGGTATC 8640 0.0 16.299917 7 ACGCAGA 7715 0.0 15.515251 14-15 AACGCAG 6340 0.0 15.477987 12-13 GTGGTAT 10160 0.0 14.188046 6 CGCTAGT 350 0.0 13.887846 90-91 AGAGTAC 8430 0.0 13.750424 18-19 ACGTACG 335 1.8189894E-12 13.44561 20-21 >>END_MODULE