FastQCFastQC Report
Sat 18 Jun 2016
SRR578568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8038250
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110670813.76802164650266No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT258950.3221472335396386No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG90660.11278574316549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81380.10124094174727087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2418000.091.941535
GGTATCA2426250.091.784468
GTATCAA2426600.091.761679
TGGTATC2429950.091.567797
GTGGTAT2434950.091.418076
AAGCAGT2444150.091.382291
CAGTGGT2442500.090.842244
GCAGTGG2453850.090.754193
AGCAGTG2461600.090.650062
TCAACGC2423100.046.47635312-13
CAACGCA2425700.046.3550512-13
AACGCAG2434050.046.34528414-15
ATCAACG2426250.046.29912610-11
ACGCAGA2436150.046.18149614-15
CGCAGAG2444400.046.16914716-17
TATCAAC2437950.046.089610-11
CAGAGTA2427700.045.9185718-19
AGAGTAC2381800.045.8662118-19
GTATCGA4250.045.8359079
GAGTACT1396050.045.66829720-21
Produced by FastQC (version 0.11.5)