##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8038250 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.65920940503219 31.0 31.0 34.0 27.0 34.0 2 30.959554505022858 31.0 31.0 34.0 27.0 34.0 3 31.04066196000373 31.0 31.0 34.0 27.0 34.0 4 34.44676179516686 37.0 35.0 37.0 30.0 37.0 5 34.54246073461263 37.0 35.0 37.0 30.0 37.0 6 34.5332982925388 37.0 35.0 37.0 30.0 37.0 7 34.49968401082325 37.0 35.0 37.0 31.0 37.0 8 34.46458283830436 37.0 35.0 37.0 30.0 37.0 9 35.8611001150748 39.0 35.0 39.0 30.0 39.0 10-11 35.81851205175256 39.0 35.0 39.0 30.0 39.0 12-13 35.88607514073337 39.0 35.0 39.0 30.0 39.0 14-15 36.963145460765716 40.0 36.0 41.0 31.0 41.0 16-17 36.91632077877647 40.0 36.5 41.0 30.5 41.0 18-19 36.904959226199736 40.0 36.5 41.0 30.5 41.0 20-21 36.7785511149815 39.5 36.0 41.0 30.0 41.0 22-23 36.461208658601066 39.0 36.0 40.5 29.5 41.0 24-25 36.37600827294498 39.0 36.0 40.5 30.0 41.0 26-27 36.28030317544241 39.0 36.0 40.5 29.0 41.0 28-29 36.02874612011321 39.0 35.0 41.0 27.0 41.0 30-31 35.788259136005976 39.0 35.0 41.0 26.5 41.0 32-33 35.37331670450658 38.0 34.0 40.0 25.0 41.0 34-35 35.16422001057444 38.0 34.0 40.0 25.0 41.0 36-37 34.82778533884863 38.0 34.0 40.0 25.0 41.0 38-39 34.07196267844369 36.5 33.0 39.5 24.0 41.0 40-41 34.23179815258297 37.0 33.0 40.0 24.0 41.0 42-43 34.14680415513327 37.0 33.0 40.0 24.0 41.0 44-45 34.003180916244204 37.0 33.0 40.0 23.0 41.0 46-47 33.78143097067148 37.0 33.0 40.0 23.0 41.0 48-49 33.409005442726965 37.0 33.0 40.0 21.0 41.0 50-51 32.13868541038161 35.5 31.5 39.0 15.5 40.5 52-53 31.68552508319597 35.5 31.0 39.0 7.0 40.0 54-55 31.199749696762353 36.0 30.0 40.0 2.0 41.0 56-57 30.658165956520385 36.0 28.5 40.0 2.0 41.0 58-59 30.310684850558268 36.0 28.0 40.0 2.0 41.0 60-61 30.006565110565113 35.5 28.0 39.0 2.0 41.0 62-63 29.666675893384756 35.0 27.5 39.0 2.0 41.0 64-65 29.320276925947812 35.0 27.0 39.0 2.0 40.5 66-67 28.989144714334586 35.0 26.0 38.0 2.0 40.0 68-69 28.653217304761608 35.0 26.0 38.0 2.0 40.0 70-71 28.27506117625105 34.5 26.0 37.0 2.0 39.5 72-73 27.87976674027307 34.0 25.5 36.5 2.0 39.0 74-75 27.515921438123968 34.0 25.0 36.0 2.0 39.0 76-77 27.12592902684042 34.0 24.0 35.5 2.0 38.5 78-79 26.76669019998134 34.0 23.5 35.0 2.0 37.0 80-81 26.409801511523032 34.0 21.5 35.0 2.0 37.0 82-83 26.031049793176376 33.0 20.0 35.0 2.0 36.5 84-85 25.684013995583616 33.0 19.5 35.0 2.0 36.0 86-87 25.359971511212017 33.0 18.0 35.0 2.0 36.0 88-89 25.009609678723603 33.0 14.5 35.0 2.0 35.0 90-91 24.649973999315772 32.0 8.5 35.0 2.0 35.0 92-93 24.30158031909931 32.0 3.5 35.0 2.0 35.0 94-95 23.87110173234224 32.0 2.0 35.0 2.0 35.0 96-97 23.38558604173794 32.0 2.0 35.0 2.0 35.0 98-99 22.85984567536466 31.0 2.0 35.0 2.0 35.0 100-101 21.549382639256056 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5992837133656863 1101 2 0.6948028569653992 1101 3 0.6820680487196817 1101 4 0.7550325039281063 1101 5 0.7093539368022519 1101 6 0.6235355561623592 1101 7 0.5249096214027276 1101 8 0.5387134162562006 1101 9 0.7761659778686365 1101 10-11 0.8135501391791635 1101 12-13 0.8340709367887911 1101 14-15 0.915193692498157 1101 16-17 0.871337259553556 1101 18-19 0.9429190445167492 1101 20-21 0.9005333008364644 1101 22-23 0.8683672368009638 1101 24-25 0.9271287064958145 1101 26-27 1.1210466766235285 1101 28-29 1.1049991373851142 1101 30-31 1.1138367932343343 1101 32-33 1.093591721714894 1101 34-35 1.2307468643132324 1101 36-37 1.1700020010926195 1101 38-39 1.0960930005575733 1101 40-41 1.2247303951967723 1101 42-43 1.4343830842291183 1101 44-45 1.3687229135850885 1101 46-47 1.3768726201050612 1101 48-49 1.23874884028681 1101 50-51 1.2307984247405557 1101 52-53 1.3224016005139383 1101 54-55 1.1276207161870389 1101 56-57 0.9886736562553509 1101 58-59 0.9448453339323812 1101 60-61 1.0662447809200586 1101 62-63 1.0079273435073581 1101 64-65 0.9099962578590208 1101 66-67 1.0093440971087482 1101 68-69 0.9584664048726417 1101 70-71 1.0341893372753326 1101 72-73 1.1040069476946464 1101 74-75 1.0627378460152244 1101 76-77 1.1176790586071057 1101 78-79 1.0934242654916453 1101 80-81 1.1598925246330758 1101 82-83 1.197280392242373 1101 84-85 1.1144514962405871 1101 86-87 1.1658866554213994 1101 88-89 1.2791908005597108 1101 90-91 1.2938697396307575 1101 92-93 1.342318254048383 1101 94-95 1.4208381447904692 1101 96-97 1.5414959105753958 1101 98-99 1.8482898499112785 1101 100-101 2.132621339851795 1102 1 0.5361798139086602 1102 2 0.5161705626937483 1102 3 0.47657246965188094 1102 4 0.6134295806825492 1102 5 0.5352958905635248 1102 6 0.5000838151186642 1102 7 0.38528659832719114 1102 8 0.5776685620483661 1102 9 0.6236252076416378 1102 10-11 0.6995061365285551 1102 12-13 0.5965193947773457 1102 14-15 0.6417556530576718 1102 16-17 0.636439668489345 1102 18-19 0.629885244564008 1102 20-21 0.7954270442003235 1102 22-23 0.7796612292364316 1102 24-25 0.6931115415181566 1102 26-27 0.9023206710842615 1102 28-29 0.8333857428873088 1102 30-31 1.0166261021050431 1102 32-33 0.8797959320080722 1102 34-35 0.9147435546099203 1102 36-37 0.8626354462693371 1102 38-39 0.8918067822829627 1102 40-41 0.9923454679374046 1102 42-43 1.2114211514429272 1102 44-45 1.1215671843163335 1102 46-47 1.2601908330666163 1102 48-49 1.1158790361627382 1102 50-51 1.126583845642557 1102 52-53 1.1085281165730088 1102 54-55 0.9621929108300478 1102 56-57 1.0793198211980801 1102 58-59 0.8458918447684205 1102 60-61 1.0105053192368771 1102 62-63 1.10832431248474 1102 64-65 0.9502304864328508 1102 66-67 1.0691487156519734 1102 68-69 1.0327113658508296 1102 70-71 1.0194439916751534 1102 72-73 1.1008183307289947 1102 74-75 1.1133952934990745 1102 76-77 1.0475503079505266 1102 78-79 0.9569888674774667 1102 80-81 0.9985431187985441 1102 82-83 1.0038305728281713 1102 84-85 0.9826382167444656 1102 86-87 1.0929739921495134 1102 88-89 1.1475086226813644 1102 90-91 1.2677301230328517 1102 92-93 1.4631248454448134 1102 94-95 1.3655588748793974 1102 96-97 1.4517817063283864 1102 98-99 1.6414973485892332 1102 100-101 1.8767603846983008 1103 1 0.4371895503090997 1103 2 0.5019612467271024 1103 3 0.4960805532386168 1103 4 0.6106148045502309 1103 5 0.5249454822234867 1103 6 0.3929609240371974 1103 7 0.35312792525292735 1103 8 0.3904939377870278 1103 9 0.4326637205309183 1103 10-11 0.5629590563021836 1103 12-13 0.47613623343181644 1103 14-15 0.5800763736237897 1103 16-17 0.5443659993760548 1103 18-19 0.563496056310413 1103 20-21 0.6382562958592501 1103 22-23 0.46812426412637365 1103 24-25 0.5409579278512453 1103 26-27 0.7488003517202699 1103 28-29 0.7553301456220467 1103 30-31 0.8279015165659231 1103 32-33 0.8821468551649971 1103 34-35 0.8090023847181271 1103 36-37 0.8905941825122241 1103 38-39 0.8740856162818886 1103 40-41 0.773597663860734 1103 42-43 0.9061523038560964 1103 44-45 0.8206083748451789 1103 46-47 0.7705903561213177 1103 48-49 0.9420920919486235 1103 50-51 0.9602421817928288 1103 52-53 0.8048823105924079 1103 54-55 0.6022917467965172 1103 56-57 0.5640288719896489 1103 58-59 0.46222958831711836 1103 60-61 0.5402960935111238 1103 62-63 0.5435569453663014 1103 64-65 0.6161778271743259 1103 66-67 0.7020073843621226 1103 68-69 0.6717627417655336 1103 70-71 0.6517149111040936 1103 72-73 0.647479565682989 1103 74-75 0.5806185442866472 1103 76-77 0.5441918674314756 1103 78-79 0.5699133232768148 1103 80-81 0.5929474438521538 1103 82-83 0.662339068416351 1103 84-85 0.7544240691917317 1103 86-87 0.7965062943203982 1103 88-89 0.82503024536053 1103 90-91 0.8326294119790099 1103 92-93 0.813479151895482 1103 94-95 0.9437992070842256 1103 96-97 1.0233004235467185 1103 98-99 1.1125007311432995 1103 100-101 1.3153471679581727 1104 1 0.2742858440262701 1104 2 0.2603362966826559 1104 3 0.23646184164631023 1104 4 0.3976875871780763 1104 5 0.3202565564317794 1104 6 0.2618455338457508 1104 7 0.26053696203932475 1104 8 0.2743658598413745 1104 9 0.32115102086677894 1104 10-11 0.4486201296135519 1104 12-13 0.4279809202638063 1104 14-15 0.35745836751120663 1104 16-17 0.5035653490055125 1104 18-19 0.45005469743708204 1104 20-21 0.48810473093820406 1104 22-23 0.35893319263531254 1104 24-25 0.4888726015646725 1104 26-27 0.48819052787846573 1104 28-29 0.4767699947276114 1104 30-31 0.46162484383058455 1104 32-33 0.5286096345668696 1104 34-35 0.5488552250018159 1104 36-37 0.6405030494718034 1104 38-39 0.5917339897820781 1104 40-41 0.6440884677753118 1104 42-43 0.7016467910276205 1104 44-45 0.6961950419549083 1104 46-47 0.5906085415592628 1104 48-49 0.6874546876434593 1104 50-51 0.4908839095505897 1104 52-53 0.49950299099951323 1104 54-55 0.6308232844452846 1104 56-57 0.6223664277126915 1104 58-59 0.6770008602655828 1104 60-61 0.6143176190235238 1104 62-63 0.808420467830345 1104 64-65 0.8158189117957164 1104 66-67 0.5873270690106374 1104 68-69 0.5585933704658856 1104 70-71 0.5803046456831567 1104 72-73 0.609248755304705 1104 74-75 0.6859891887433989 1104 76-77 0.7707779543363422 1104 78-79 0.7004877231558915 1104 80-81 0.7013358299915815 1104 82-83 0.6845705898617318 1104 84-85 0.7974166323945084 1104 86-87 0.7396152219765249 1104 88-89 0.861723733391635 1104 90-91 0.851468631710798 1104 92-93 0.9193394122142635 1104 94-95 0.9457687570622753 1104 96-97 0.8937408675607728 1104 98-99 1.1207393609070166 1104 100-101 1.3244415043293714 1105 1 -0.04045544588830907 1105 2 -0.011795566917943034 1105 3 0.14194351516938752 1105 4 0.14341884561532936 1105 5 0.22657680477897912 1105 6 0.25231979262266435 1105 7 0.31285191250922395 1105 8 0.29079706997864463 1105 9 0.19448510589531054 1105 10-11 0.11557334260184149 1105 12-13 0.10605087052320528 1105 14-15 0.010170591328567014 1105 16-17 0.07507472017331906 1105 18-19 0.011090033537868749 1105 20-21 0.30986618324030246 1105 22-23 0.27692772455863235 1105 24-25 0.28548765738619153 1105 26-27 0.5170015930191241 1105 28-29 0.44421412163767116 1105 30-31 0.44585019129615944 1105 32-33 0.6012626803741625 1105 34-35 0.5475660827274424 1105 36-37 0.6119257555419111 1105 38-39 0.6630401952751228 1105 40-41 0.5508513722243791 1105 42-43 0.5837757660726695 1105 44-45 0.6502296441411204 1105 46-47 0.6671236322244383 1105 48-49 0.7855408214582837 1105 50-51 0.682686794272712 1105 52-53 0.546513656396467 1105 54-55 0.5899576289210771 1105 56-57 0.5394232329927391 1105 58-59 0.5616409928412303 1105 60-61 0.5226130626658048 1105 62-63 0.5424001840886419 1105 64-65 0.5001963004819245 1105 66-67 0.6540474747641589 1105 68-69 0.550094137234332 1105 70-71 0.46367276552915015 1105 72-73 0.6240996490116508 1105 74-75 0.5251418223455566 1105 76-77 0.40971323242431623 1105 78-79 0.47182458758303625 1105 80-81 0.4300983703218897 1105 82-83 0.3900700842942513 1105 84-85 0.4764561795247566 1105 86-87 0.530943881084756 1105 88-89 0.5723308112014749 1105 90-91 0.5714100045441768 1105 92-93 0.5855008439665319 1105 94-95 0.6550244557203371 1105 96-97 0.7404317514784395 1105 98-99 0.8551166680908757 1105 100-101 1.0713296042505718 1106 1 0.05713912514017849 1106 2 0.14800693221184957 1106 3 0.19873518132658674 1106 4 0.3444688321373519 1106 5 0.21046908058044522 1106 6 0.2306258314179246 1106 7 0.2465329211711378 1106 8 0.08726190278050439 1106 9 0.015556107515919848 1106 10-11 0.1553892507196153 1106 12-13 0.17854711346016927 1106 14-15 0.12407365399817394 1106 16-17 0.19495149063705242 1106 18-19 0.1086708629757851 1106 20-21 0.1727458760075038 1106 22-23 0.3014912944466204 1106 24-25 0.23945013196284748 1106 26-27 0.15449684936597663 1106 28-29 0.18231039869864674 1106 30-31 0.3764195289503718 1106 32-33 0.3368219232876868 1106 34-35 0.3201820766151826 1106 36-37 0.37961998843753264 1106 38-39 0.5865743994401598 1106 40-41 0.2574145269480681 1106 42-43 0.2420840074485895 1106 44-45 0.38211588609429725 1106 46-47 0.39442877843328006 1106 48-49 0.33557041916171926 1106 50-51 0.38970412258668574 1106 52-53 0.4535210599167563 1106 54-55 0.15012225261519063 1106 56-57 0.17478026980326433 1106 58-59 0.463088450663804 1106 60-61 0.3870809607958563 1106 62-63 0.31275879921899374 1106 64-65 0.41947107429814423 1106 66-67 0.5483092305266268 1106 68-69 0.5723603375319826 1106 70-71 0.44983905021699044 1106 72-73 0.4620086842813187 1106 74-75 0.5162865310535629 1106 76-77 0.45304840395245805 1106 78-79 0.4371073844689768 1106 80-81 0.4242630319315772 1106 82-83 0.4332743461391928 1106 84-85 0.5295964666937145 1106 86-87 0.6311726589368583 1106 88-89 0.5053800993141664 1106 90-91 0.5171023832785657 1106 92-93 0.5567690009544997 1106 94-95 0.5662106769321227 1106 96-97 0.6206522658671219 1106 98-99 0.6260162111184897 1106 100-101 0.9115746117624575 1107 1 0.0785816436655864 1107 2 0.05899346680806872 1107 3 0.2510140705066668 1107 4 0.3980723712584293 1107 5 0.17066304767627827 1107 6 0.232712900770224 1107 7 0.248491558271418 1107 8 0.1582092824589978 1107 9 0.1419303938834986 1107 10-11 0.2306553894164196 1107 12-13 0.21274177932325955 1107 14-15 0.27521042494706904 1107 16-17 0.20350067407640893 1107 18-19 0.35528277477125414 1107 20-21 0.19744511922780816 1107 22-23 0.2941329048252541 1107 24-25 0.47416105426042776 1107 26-27 0.20288394052516168 1107 28-29 0.2446783702600257 1107 30-31 0.3474846115032193 1107 32-33 0.5189149192112197 1107 34-35 0.32962830147564404 1107 36-37 0.37271592285979693 1107 38-39 0.5197300215087282 1107 40-41 0.25884927659638635 1107 42-43 0.36695245594855663 1107 44-45 0.5467972465543198 1107 46-47 0.3705808323049169 1107 48-49 0.45897846776851736 1107 50-51 0.3109016339280757 1107 52-53 0.5642405785276701 1107 54-55 0.4497425746831176 1107 56-57 0.3851761455446798 1107 58-59 0.615265845919037 1107 60-61 0.5670495399421824 1107 62-63 0.47249975124134025 1107 64-65 0.5913292883225623 1107 66-67 0.6873365584812987 1107 68-69 0.5764882863005596 1107 70-71 0.43433347720695537 1107 72-73 0.4986383688278728 1107 74-75 0.6494161408217103 1107 76-77 0.5781398717229642 1107 78-79 0.43762906477446606 1107 80-81 0.44670253314153996 1107 82-83 0.3732898699681577 1107 84-85 0.42744753457662554 1107 86-87 0.582113390718014 1107 88-89 0.5490510058372706 1107 90-91 0.4904243670980257 1107 92-93 0.5741431567035669 1107 94-95 0.5319844077950506 1107 96-97 0.6363400433444255 1107 98-99 0.6333270809458647 1107 100-101 0.7185926969953513 1108 1 0.12923133184009217 1108 2 0.15555230164089906 1108 3 0.1439089813656267 1108 4 0.34118594127799895 1108 5 0.15810040869929765 1108 6 0.2591311429811398 1108 7 0.2709092826035757 1108 8 0.20043670135591896 1108 9 0.22586739385116772 1108 10-11 0.22294616523999622 1108 12-13 0.07915311284604343 1108 14-15 0.2577193360496821 1108 16-17 0.1777936965878979 1108 18-19 0.14855971671084944 1108 20-21 0.25169274139896913 1108 22-23 0.19756166873352043 1108 24-25 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0.1361535353353247 2313 1 -0.693886846257211 2313 2 -0.4846646591766408 2313 3 -0.23008760960381025 2313 4 0.027037427347963217 2313 5 -0.3444700472596267 2313 6 -0.20709573173861884 2313 7 -0.23161857676262088 2313 8 -0.15397843412296197 2313 9 -0.03261372622040426 2313 10-11 -0.26068410534854536 2313 12-13 -0.22914935130575742 2313 14-15 -0.6158555377921857 2313 16-17 -0.3737851845372617 2313 18-19 -0.3062714970732259 2313 20-21 -0.2874652827837352 2313 22-23 -0.28195348321930425 2313 24-25 -0.5060141899261552 2313 26-27 -0.3971796151649656 2313 28-29 -0.4088906551167426 2313 30-31 -0.5624476616238141 2313 32-33 -0.8592853155523841 2313 34-35 -0.4578166562170338 2313 36-37 -0.32671329553330253 2313 38-39 -0.27204209209678964 2313 40-41 -0.1899420615152536 2313 42-43 -0.3564799097048805 2313 44-45 -0.34852037246201917 2313 46-47 -0.37860777656404565 2313 48-49 -0.3998735562158586 2313 50-51 -0.38754863061021894 2313 52-53 -0.3832422741089836 2313 54-55 -0.25852436630139053 2313 56-57 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-1.5776648385243845 2315 34-35 -1.595944373476101 2315 36-37 -1.2981631283441004 2315 38-39 -1.462684098645326 2315 40-41 -2.4344144457515 2315 42-43 -1.9674555508285678 2315 44-45 -1.66593607921007 2315 46-47 -1.3619178795192255 2315 48-49 -1.7376127132485735 2315 50-51 -1.5714546362133177 2315 52-53 -1.3514107493851384 2315 54-55 -1.4632601239361307 2315 56-57 -1.5132080015955722 2315 58-59 -1.6900643550363945 2315 60-61 -1.357522078268694 2315 62-63 -1.2190160343712613 2315 64-65 -1.2192293171288213 2315 66-67 -1.2796151196406704 2315 68-69 -1.4994575317129417 2315 70-71 -1.2679423758274666 2315 72-73 -1.139235748007497 2315 74-75 -1.250757129747619 2315 76-77 -1.259741511297566 2315 78-79 -1.4513137227507542 2315 80-81 -1.4840093259363876 2315 82-83 -1.277652760355938 2315 84-85 -1.40552620410271 2315 86-87 -1.486339243710777 2315 88-89 -1.4796969570982021 2315 90-91 -1.4100228875590588 2315 92-93 -1.4207238773479425 2315 94-95 -1.4184045951131736 2315 96-97 -1.563802066469293 2315 98-99 -1.56143518729165 2315 100-101 -1.7625895602380837 2316 1 -0.9017213662693422 2316 2 -1.0664090940585673 2316 3 -1.5388091901938843 2316 4 -1.3528650674819858 2316 5 -0.84857404587585 2316 6 -0.8287330521567071 2316 7 -1.052275871506538 2316 8 -1.1068534732062858 2316 9 -1.340677372387674 2316 10-11 -1.2727191078949147 2316 12-13 -1.112348720516536 2316 14-15 -1.1524267525717775 2316 16-17 -1.9000984164712946 2316 18-19 -1.2771870092508237 2316 20-21 -1.6617172906287436 2316 22-23 -1.2004572913664404 2316 24-25 -1.11787691719001 2316 26-27 -1.1248714315202122 2316 28-29 -1.5258721656925545 2316 30-31 -1.2993335537295039 2316 32-33 -1.4536216939845303 2316 34-35 -1.8730508557077101 2316 36-37 -1.6519594484351359 2316 38-39 -1.555166375471245 2316 40-41 -1.9548342327041368 2316 42-43 -2.105291315202855 2316 44-45 -2.397560401426304 2316 46-47 -2.1528179612866083 2316 48-49 -2.795853245000316 2316 50-51 -2.3530098367318075 2316 52-53 -3.0968144579315258 2316 54-55 -4.20188224182435 2316 56-57 -3.007935060569821 2316 58-59 -2.5523251439974253 2316 60-61 -2.2890362594456164 2316 62-63 -2.8677144333131857 2316 64-65 -2.825868421531073 2316 66-67 -2.520822217200088 2316 68-69 -2.5079280069057717 2316 70-71 -2.723049943266716 2316 72-73 -5.639670019467669 2316 74-75 -3.4590636406166553 2316 76-77 -2.803057204982636 2316 78-79 -2.6669977464595256 2316 80-81 -2.672488226560759 2316 82-83 -2.6228177985831316 2316 84-85 -2.740663313305493 2316 86-87 -2.541914392455819 2316 88-89 -2.3670041273815983 2316 90-91 -2.41256069837873 2316 92-93 -2.748776344122877 2316 94-95 -2.9780297161628084 2316 96-97 -2.9873077845338543 2316 98-99 -3.3293282792667647 2316 100-101 -3.348674982658924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75614.0 3 13628.0 4 11951.0 5 12171.0 6 13814.0 7 17265.0 8 21774.0 9 25647.0 10 30415.0 11 36042.0 12 37083.0 13 42332.0 14 48505.0 15 58390.0 16 73862.0 17 98968.0 18 143171.0 19 211738.0 20 278053.0 21 200349.0 22 116575.0 23 102213.0 24 107978.0 25 115484.0 26 127410.0 27 143605.0 28 166130.0 29 193657.0 30 227645.0 31 270872.0 32 328964.0 33 403617.0 34 518793.0 35 716511.0 36 1030960.0 37 1113515.0 38 789427.0 39 114039.0 40 83.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.438867874153337 49.417310142629006 23.189616783540032 16.95420519967762 2 16.268485585485866 51.0220897587332 17.14673960071621 15.562685055064717 3 46.47616218203044 19.693206754795504 18.045348233754254 15.785282829419797 4 16.33636531448791 18.10982313782566 18.97217845583718 46.58163309184925 5 16.253639360393848 48.42802538445316 20.02121927941598 15.297115975737016 6 46.59699306596924 19.218647408887417 17.921743211375063 16.262616313768284 7 17.14494736075302 18.62456191034175 49.051821126801784 15.178669602103453 8 46.219671917642856 19.409985159607988 18.792687605078978 15.577655317670178 9 45.814273196552705 19.075417260434904 19.313215058601592 15.797094484410797 10-11 15.933737960368246 33.96121882621889 34.00698478698877 16.098058426424092 12-13 15.761009246580096 19.35916380226166 33.974454617722145 30.905372333436105 14-15 15.910938167724858 49.109509289049754 18.890300226993382 16.089252316232002 16-17 30.876851492566022 19.172651109915197 18.98535288141497 30.965144516103816 18-19 16.08354082235672 34.0443446132053 18.98281676579772 30.889297798640264 20-21 31.0725455261053 34.07479398085279 18.990493109128472 15.862167383913443 22-23 30.981698610632723 19.128774336796717 33.94825429095846 15.941272761612105 24-25 15.997457990694983 33.82307421076548 19.496006578556788 30.68346121998275 26-27 16.25275571744862 25.014838837590993 42.54922330866811 16.18318213629228 28-29 28.19146364222852 19.031967049531804 36.716414678209645 16.060154630030034 30-31 25.924527273433227 20.218363185818152 37.13241919121022 16.7246903495384 32-33 19.88817954494613 22.69332353478335 38.707275890060025 18.711221030210503 34-35 18.959369676442197 21.789927123055 39.45356076139638 19.79714243910642 36-37 18.761558684793894 21.995538514138264 39.59400055485421 19.64890224621363 38-39 19.200657885934035 22.125858025163488 39.31286666987378 19.360617419028696 40-41 19.396128087931867 21.812978625355154 39.28576518291186 19.505128103801123 42-43 19.74375181598432 22.102059298994114 39.31142044029946 18.842768444722104 44-45 19.2165134204961 22.224688057029905 39.14876339905661 19.410035123417384 46-47 19.427738012642962 22.0479949180724 38.9870296467972 19.53723742248744 48-49 19.489954026478955 22.09974785502535 39.11816464345008 19.29213347504561 50-51 19.606382448447235 22.14064812318138 38.27918927444382 19.97378015392756 52-53 19.969356788130415 22.90858243284725 37.31472521326385 19.807335565758482 54-55 20.4933390675572 23.959742114254638 35.534799036964024 20.012119781224133 56-57 21.058773534283343 24.51722955374494 34.373269463635545 20.05072744833617 58-59 20.902539758305842 25.480545820418698 33.32924877657775 20.287665644697704 60-61 21.03768865830358 25.833210632287308 32.69693704583758 20.432163663571533 62-63 20.986964049209792 25.637164511579247 33.06636134956317 20.309510089647794 64-65 20.72312493247721 25.64776451634263 32.91545125638448 20.71365929479568 66-67 20.713138911429837 25.154901951022808 33.719022098979934 20.412937038567424 68-69 20.523412317898163 24.89008532991452 34.01018861121536 20.576313740971955 70-71 20.42050779255316 24.668484388661582 34.550880542979044 20.360127275806214 72-73 20.143954371885364 24.541496090999352 34.34048168485905 20.97406785225623 74-75 20.583627011518466 24.618018179597065 33.75913325205414 21.03922155683033 76-77 21.04637601413051 25.146055516249515 33.16862136499451 20.638947104625466 78-79 20.833953561311713 25.123390103899595 33.03654283680246 21.006113497986227 80-81 20.80673154770461 25.380969183062724 32.84523202780936 20.9670672414233 82-83 21.82705822854255 25.599613530153505 32.142163951107804 20.43116429019614 84-85 21.261329377988407 26.2117148778781 31.702444304841592 20.824511439291896 86-87 21.18927149158419 25.988810369423597 31.70716452625355 21.114753612738664 88-89 21.116602546678653 25.75200350071239 31.55020774278725 21.58118620982171 90-91 21.130947787761734 25.78710159523704 31.71797515466725 21.363975462333975 92-93 21.173021330800243 26.111523025422283 31.680318955466895 21.035136688310576 94-95 21.31684625048905 25.869828057168537 31.202680063666072 21.61064562867634 96-97 21.097844104720977 25.759274112836483 31.90430582354734 21.238575958895197 98-99 21.274473335061536 26.034677866874617 31.254104531153494 21.436744266910356 100-101 21.81191386365759 25.741969880390247 31.01842343443489 21.427692821517276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9787.0 1 6088.0 2 1716.5 3 952.5 4 802.0 5 745.0 6 843.0 7 984.5 8 943.5 9 966.5 10 1379.5 11 9687.0 12 178573.5 13 199413.0 14 51721.0 15 45131.5 16 48332.0 17 51925.5 18 54168.0 19 55495.5 20 56778.0 21 58064.5 22 59565.0 23 61830.0 24 64732.5 25 68741.0 26 74070.0 27 80143.5 28 86076.5 29 91755.0 30 97863.0 31 105052.0 32 113604.5 33 124128.5 34 142711.0 35 154137.0 36 157393.0 37 166091.0 38 174585.5 39 183525.5 40 193081.5 41 200720.5 42 206568.0 43 216628.5 44 229363.0 45 236258.0 46 239495.5 47 247092.5 48 254067.5 49 257001.0 50 258469.5 51 257868.5 52 257376.0 53 262034.5 54 259080.0 55 244272.0 56 239879.5 57 227710.0 58 213599.0 59 182951.5 60 133724.0 61 103316.0 62 79622.0 63 60960.0 64 44990.5 65 30393.5 66 18922.5 67 11275.5 68 7048.0 69 4549.0 70 3110.5 71 2334.0 72 2012.0 73 1727.5 74 1516.5 75 1430.0 76 1347.0 77 1252.0 78 1185.0 79 1120.5 80 1025.0 81 922.0 82 842.0 83 764.5 84 702.0 85 606.5 86 507.5 87 437.5 88 353.0 89 281.5 90 240.0 91 195.5 92 133.0 93 91.0 94 63.5 95 44.5 96 33.0 97 21.5 98 15.0 99 8.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09835474139271608 2 0.08109974185923553 3 0.08680993997449693 4 0.1893820172301185 5 0.07379715734146114 6 0.09055453612415638 7 0.09510776599384194 8 0.08448356296457563 9 0.07733026467203682 10-11 0.07563213385998196 12-13 0.06972288744440643 14-15 0.0756072528224427 16-17 0.06641992971106896 18-19 0.05837713432650141 20-21 0.053344944484184996 22-23 0.04326190402139769 24-25 0.045544739215625296 26-27 0.04467390290175101 28-29 0.043834167884800795 30-31 0.03406214039125432 32-33 0.03791248095045563 34-35 0.05088794202718253 36-37 0.0461170030790284 38-39 0.05014151090100458 40-41 0.04786489596616179 42-43 0.04597393711317762 44-45 0.049874039747457466 46-47 0.043585357509408146 48-49 0.03968525487512829 50-51 0.043498273878020716 52-53 0.03970391565328274 54-55 0.04293845053338724 56-57 0.041669517618884704 58-59 0.048797934873884244 60-61 0.04748546014368799 62-63 0.052231518054302865 64-65 0.04884147668957796 66-67 0.0434174105060181 68-69 0.04347339284048145 70-71 0.05226883961061176 72-73 0.039859422137903146 74-75 0.04731751314029795 76-77 0.046272509563648805 78-79 0.04441265200758872 80-81 0.0441016390383479 82-83 0.04317482039001026 84-85 0.04419494292912014 86-87 0.04897210213665909 88-89 0.041227879202562746 90-91 0.04456193823282431 92-93 0.04071159767362299 94-95 0.04373464373464374 96-97 0.02986346530650328 98-99 0.03636363636363636 100-101 0.024762852610953876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8038250.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.47117018805504 #Duplication Level Percentage of deduplicated Percentage of total 1 91.5857371411257 40.72924903201475 2 4.5427476520047065 4.040426079073775 3 1.284661548702248 1.7139120709916407 4 0.6317374980604634 1.1237642316169185 5 0.37935537514950646 0.8435188725013584 6 0.24210684866755647 0.6460064922473153 7 0.17487287874622437 0.544376108639892 8 0.13536282042143624 0.48157944192774593 9 0.10943007507941986 0.4379835143293669 >10 0.8608498995759807 7.201282485071825 >50 0.031470393048330526 0.9847832083726672 >100 0.013215439229926173 1.3430842638619407 >500 0.0035658689857675668 1.1139249258114508 >1k 0.0040941458725475945 3.481529304533594 >5k 6.603461084754184E-4 2.0541478075392887 >10k+ 1.320692216950837E-4 33.26043216146651 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1106708 13.76802164650266 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 25895 0.3221472335396386 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9066 0.11278574316549 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8138 0.10124094174727087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.2440518769632694E-5 0.0 6 0.0 0.0 0.0 1.2440518769632694E-5 0.0 7 0.0 0.0 0.0 1.2440518769632694E-5 0.0 8 0.0 0.0 0.0 1.2440518769632694E-5 0.0 9 0.0 0.0 0.0 1.2440518769632694E-5 0.0 10-11 0.0 1.2440518769632694E-5 0.0 1.2440518769632694E-5 0.0 12-13 0.0 2.488103753926539E-5 0.0 1.2440518769632694E-5 0.0 14-15 0.0 2.488103753926539E-5 0.0 1.2440518769632694E-5 0.0 16-17 0.0 3.1101296924081736E-5 0.0 1.2440518769632694E-5 0.0 18-19 0.0 3.732155630889808E-5 0.0 1.2440518769632694E-5 0.0 20-21 0.0 3.732155630889808E-5 0.0 1.2440518769632694E-5 0.0 22-23 0.0 3.732155630889808E-5 0.0 1.866077815444904E-5 0.0 24-25 0.0 3.732155630889808E-5 0.0 2.488103753926539E-5 0.0 26-27 0.0 3.732155630889808E-5 0.0 3.1101296924081736E-5 0.0 28-29 0.0 3.732155630889808E-5 0.0 4.976207507853078E-5 0.0 30-31 0.0 4.354181569371443E-5 0.0 4.976207507853078E-5 0.0 32-33 0.0 4.976207507853078E-5 0.0 4.976207507853078E-5 0.0 34-35 0.0 4.976207507853078E-5 0.0 5.598233446334712E-5 0.0 36-37 1.2440518769632694E-5 4.976207507853078E-5 0.0 6.220259384816347E-5 0.0 38-39 4.354181569371443E-5 4.976207507853078E-5 0.0 9.33038907722452E-5 0.0 40-41 6.220259384816347E-5 5.598233446334712E-5 0.0 9.952415015706155E-5 0.0 42-43 6.220259384816347E-5 6.220259384816347E-5 0.0 1.057444095418779E-4 0.0 44-45 9.952415015706155E-5 6.220259384816347E-5 0.0 1.2440518769632695E-4 0.0 46-47 1.3684570646595962E-4 7.464311261779616E-5 0.0 1.3062544708114328E-4 0.0 48-49 1.61726744005225E-4 8.08633720026125E-5 0.0 1.3684570646595962E-4 0.0 50-51 1.9282804092930674E-4 8.708363138742885E-5 0.0 1.3684570646595962E-4 0.0 52-53 2.114888190837558E-4 8.708363138742885E-5 0.0 1.3684570646595962E-4 0.0 54-55 2.6125089416228657E-4 8.708363138742885E-5 0.0 1.4928622523559232E-4 0.0 56-57 3.296737473952664E-4 8.708363138742885E-5 0.0 1.6172674400522503E-4 0.0 58-59 3.856560818586135E-4 8.708363138742885E-5 0.0 1.6794700339004136E-4 0.0 60-61 4.789599726308587E-4 9.952415015706155E-5 0.0 1.741672627748577E-4 0.0 62-63 5.971449009423693E-4 9.952415015706155E-5 0.0 1.866077815444904E-4 0.0 64-65 7.650919043324107E-4 9.952415015706155E-5 0.0 1.866077815444904E-4 0.0 66-67 9.205983889528193E-4 1.057444095418779E-4 0.0 1.9282804092930674E-4 0.0 68-69 0.0010698846141884116 1.4306596585077599E-4 0.0 1.990483003141231E-4 0.0 70-71 0.0011942898018847386 1.6172674400522503E-4 0.0 2.1148881908375578E-4 0.0 72-73 0.001418219139738127 1.6172674400522503E-4 0.0 2.2392933785338848E-4 0.0 74-75 0.0016483687369763318 1.6172674400522503E-4 0.0 2.3014959723820482E-4 0.0 76-77 0.001934500668677884 1.741672627748577E-4 0.0 2.363698566230212E-4 0.0 78-79 0.0021833110440705374 1.8038752215967407E-4 0.0 2.363698566230212E-4 0.0 80-81 0.0026498304979317637 1.9282804092930674E-4 0.0 2.6125089416228657E-4 0.0 82-83 0.003103909433023357 1.990483003141231E-4 0.0 2.6125089416228657E-4 0.0 84-85 0.0036886138151960937 1.990483003141231E-4 0.0 2.674711535471029E-4 0.0 86-87 0.00461543246353373 2.0526855969893944E-4 0.0 2.7369141293191924E-4 0.0 88-89 0.006058532640811121 2.1148881908375578E-4 0.0 2.7369141293191924E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 241800 0.0 91.94153 5 GGTATCA 242625 0.0 91.78446 8 GTATCAA 242660 0.0 91.76167 9 TGGTATC 242995 0.0 91.56779 7 GTGGTAT 243495 0.0 91.41807 6 AAGCAGT 244415 0.0 91.38229 1 CAGTGGT 244250 0.0 90.84224 4 GCAGTGG 245385 0.0 90.75419 3 AGCAGTG 246160 0.0 90.65006 2 TCAACGC 242310 0.0 46.476353 12-13 CAACGCA 242570 0.0 46.35505 12-13 AACGCAG 243405 0.0 46.345284 14-15 ATCAACG 242625 0.0 46.299126 10-11 ACGCAGA 243615 0.0 46.181496 14-15 CGCAGAG 244440 0.0 46.169147 16-17 TATCAAC 243795 0.0 46.0896 10-11 CAGAGTA 242770 0.0 45.91857 18-19 AGAGTAC 238180 0.0 45.86621 18-19 GTATCGA 425 0.0 45.835907 9 GAGTACT 139605 0.0 45.668297 20-21 >>END_MODULE