FastQCFastQC Report
Sat 18 Jun 2016
SRR578568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8038250
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220601.5184897210213666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222670.2770130314434112No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87600.1089789444219824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.027.8658313
CGCGTAG2200.024.82949654-55
CTCGCGT2300.023.7510652-53
CGTACGC2850.019.99877720-21
CAACGCA59100.019.8507312-13
ATCAACG60900.019.5759810-11
ACGTACG3400.018.16065620-21
TCAACGC57200.018.10204110-11
ACTCGCG3050.017.91108350-51
CGTAGCG3100.016.85433456-57
AACGCAG61550.016.7069412-13
GGTATCA72150.016.6553578
GTATCAA75950.016.0096559
AGTGGTA75800.015.9161135
TCGCGTA3450.015.8340452-53
ACGCAGA75550.015.65423814-15
TGGTATC85000.014.5844957
TATCAAC82350.014.361699
CGCTAGT3900.014.0058690-91
GTGGTAT89750.013.60092456
Produced by FastQC (version 0.11.5)