##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8038250 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477335862905484 31.0 31.0 34.0 30.0 34.0 2 31.79097241314963 31.0 31.0 34.0 30.0 34.0 3 31.861621621621623 31.0 31.0 34.0 30.0 34.0 4 35.53714751345131 37.0 35.0 37.0 33.0 37.0 5 35.359157030448166 37.0 35.0 37.0 33.0 37.0 6 35.356035953099244 37.0 35.0 37.0 32.0 37.0 7 35.334104562560256 37.0 35.0 37.0 32.0 37.0 8 35.32097745155973 37.0 35.0 37.0 32.0 37.0 9 36.88849451062109 39.0 37.0 39.0 33.0 39.0 10-11 36.854157621372806 39.0 37.0 39.0 32.5 39.0 12-13 36.83269013777874 39.0 37.0 39.0 32.0 39.0 14-15 38.01099256679004 40.0 37.0 41.0 33.0 41.0 16-17 37.95702746244518 40.0 37.0 41.0 33.0 41.0 18-19 37.882714770005904 40.0 37.0 41.0 32.5 41.0 20-21 37.82609541877896 40.0 37.0 41.0 32.0 41.0 22-23 37.732523621435014 40.0 37.0 41.0 32.0 41.0 24-25 37.64211358193637 39.0 36.0 41.0 32.0 41.0 26-27 37.45535645196405 39.0 36.0 41.0 32.0 41.0 28-29 37.28258352253289 39.0 36.0 41.0 31.0 41.0 30-31 37.147456784747924 39.0 36.0 41.0 31.0 41.0 32-33 37.017044879171465 39.0 36.0 40.5 31.0 41.0 34-35 36.8619555251454 39.0 35.5 40.0 30.0 41.0 36-37 36.71144770316922 39.0 35.0 40.0 30.0 41.0 38-39 36.541531676670914 38.0 35.0 40.0 30.0 41.0 40-41 36.36943899480609 38.0 35.0 40.0 30.0 41.0 42-43 36.3078088514291 38.0 35.0 40.0 30.0 41.0 44-45 36.34175672565546 38.0 35.0 40.0 30.0 41.0 46-47 36.21021957515629 38.0 35.0 40.0 30.0 41.0 48-49 36.06119951481976 38.0 35.0 40.0 29.0 41.0 50-51 35.8566481510279 38.0 34.5 40.0 28.5 41.0 52-53 35.920009828009825 38.0 34.5 40.0 28.0 41.0 54-55 35.90503312288122 38.0 34.5 40.0 28.0 41.0 56-57 35.7364833141542 38.0 34.0 40.0 28.0 41.0 58-59 35.51273753615526 38.0 34.0 40.0 28.0 41.0 60-61 35.237588965259846 37.5 34.0 40.0 28.0 41.0 62-63 34.93966510123472 37.0 34.0 40.0 27.0 41.0 64-65 34.602279414051566 37.0 33.0 39.5 26.5 41.0 66-67 34.242685596989396 36.0 33.0 39.0 26.0 41.0 68-69 33.8622639256057 36.0 32.5 39.0 26.0 40.5 70-71 33.463776941498466 35.0 32.0 38.5 26.0 40.0 72-73 33.06116349951793 35.0 32.0 37.5 25.5 40.0 74-75 32.64572183000031 35.0 31.5 37.0 25.0 39.0 76-77 31.30215594190278 34.0 29.5 36.0 23.0 38.5 78-79 31.94309980406183 34.5 31.0 36.0 24.0 39.0 80-81 31.82429595994153 34.5 31.0 36.0 24.0 37.5 82-83 31.522608963393772 34.0 31.0 35.5 24.0 37.0 84-85 31.21043224582465 34.0 31.0 35.0 23.0 37.0 86-87 30.87071352595403 34.0 31.0 35.0 21.5 36.0 88-89 30.55599664105993 34.0 30.5 35.0 20.0 36.0 90-91 30.234113706341553 34.0 30.0 35.0 19.5 36.0 92-93 29.87459092464156 34.0 30.0 35.0 17.5 35.0 94-95 29.50113669019998 34.0 29.5 35.0 11.0 35.0 96-97 29.103111684757256 34.0 29.0 35.0 2.0 35.0 98-99 28.62006661897801 34.0 29.0 35.0 2.0 35.0 100-101 27.490453830124714 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3674041048537404 1101 2 0.40638168468911573 1101 3 0.3550507499154456 1101 4 0.31945852780500417 1101 5 0.333652600864653 1101 6 0.33376453388248706 1101 7 0.32373133613310046 1101 8 0.30526472300895335 1101 9 0.4010473841363762 1101 10-11 0.40547574095386807 1101 12-13 0.3901456658939608 1101 14-15 0.5203870665020531 1101 16-17 0.48022646301245686 1101 18-19 0.4694277815719161 1101 20-21 0.4982227281230607 1101 22-23 0.5298381145406168 1101 24-25 0.5323528847112229 1101 26-27 0.4155489151274665 1101 28-29 0.543273655925006 1101 30-31 0.541032843037442 1101 32-33 0.5355871501832397 1101 34-35 0.4959419749497158 1101 36-37 0.5129367517342871 1101 38-39 0.5697700571207562 1101 40-41 0.5505831551569784 1101 42-43 0.539645390378773 1101 44-45 0.529517574455987 1101 46-47 0.5356171334275004 1101 48-49 0.5079058051895018 1101 50-51 0.49402969937467844 1101 52-53 0.5185792361060138 1101 54-55 0.4862006465876405 1101 56-57 0.5970760907296722 1101 58-59 0.5760449776624625 1101 60-61 0.6646367457998394 1101 62-63 0.5905677332461678 1101 64-65 0.6393025153709573 1101 66-67 0.6072416580175428 1101 68-69 0.6509128313993813 1101 70-71 0.5681334304945764 1101 72-73 0.6523615634615538 1101 74-75 0.6171120663177234 1101 76-77 0.48668523103328454 1101 78-79 0.5815848778569688 1101 80-81 0.572708492135618 1101 82-83 0.5605578529788495 1101 84-85 0.5896256389717358 1101 86-87 0.5401765037537487 1101 88-89 0.4438925371313083 1101 90-91 0.5643894351016101 1101 92-93 0.6551542951342348 1101 94-95 0.7182911900692126 1101 96-97 0.5327467504946561 1101 98-99 0.6098550071215882 1101 100-101 0.7245690203534494 1102 1 0.29293732002709305 1102 2 0.29356588161083863 1102 3 0.3459161405095905 1102 4 0.24279169927535094 1102 5 0.3119240936539498 1102 6 0.19533329120703513 1102 7 0.2372990322681119 1102 8 0.1805783424173555 1102 9 0.3429465462585455 1102 10-11 0.2758877543063747 1102 12-13 0.19862387153614947 1102 14-15 0.41428592986856927 1102 16-17 0.4109090234401549 1102 18-19 0.46748991149538455 1102 20-21 0.42859775380894405 1102 22-23 0.34826446875644734 1102 24-25 0.320815942346492 1102 26-27 0.3895814827501667 1102 28-29 0.37228490944922044 1102 30-31 0.37156030368549864 1102 32-33 0.4006812093575789 1102 34-35 0.41461489140294816 1102 36-37 0.37531011368578504 1102 38-39 0.5087771150378941 1102 40-41 0.3943681864034474 1102 42-43 0.3962525314382219 1102 44-45 0.38898188840465053 1102 46-47 0.3613892420857283 1102 48-49 0.3880787784921793 1102 50-51 0.4146411817178901 1102 52-53 0.49593801410362204 1102 54-55 0.4399931556080432 1102 56-57 0.4473568514580819 1102 58-59 0.5070021179224682 1102 60-61 0.49286124897423633 1102 62-63 0.4529442485650179 1102 64-65 0.48096275439915814 1102 66-67 0.4924394185641461 1102 68-69 0.38486915711350633 1102 70-71 0.47246235987157803 1102 72-73 0.523739543541744 1102 74-75 0.4302350932424446 1102 76-77 0.3959376623831474 1102 78-79 0.5113273572979011 1102 80-81 0.44182073335963423 1102 82-83 0.4309110182786604 1102 84-85 0.42985324251321444 1102 86-87 0.44749667594833653 1102 88-89 0.5836997657152061 1102 90-91 0.6492857307684403 1102 92-93 0.5012571032004587 1102 94-95 0.4382003079494865 1102 96-97 0.46482295864887746 1102 98-99 0.5727522869943407 1102 100-101 0.726810746243217 1103 1 0.2174523676957314 1103 2 0.2566649017574818 1103 3 0.3139498580570752 1103 4 0.14446847859272793 1103 5 0.24608359960726034 1103 6 0.17406647182122015 1103 7 0.14679437117813166 1103 8 0.1806350018622851 1103 9 0.2834459926084847 1103 10-11 0.31231156695445605 1103 12-13 0.306439213523241 1103 14-15 0.38133602169976655 1103 16-17 0.3578290352454303 1103 18-19 0.34567835518766543 1103 20-21 0.36882155861641763 1103 22-23 0.34271650313378643 1103 24-25 0.34017420386311414 1103 26-27 0.32581301572840715 1103 28-29 0.30985910854282395 1103 30-31 0.40920247931941844 1103 32-33 0.41476888504069365 1103 34-35 0.37341851908678336 1103 36-37 0.4908184176969925 1103 38-39 0.5125275116909904 1103 40-41 0.5492778826392879 1103 42-43 0.4409867535210523 1103 44-45 0.3555000397682164 1103 46-47 0.4099651742482493 1103 48-49 0.38781193913501966 1103 50-51 0.4287801171860437 1103 52-53 0.4554249946359974 1103 54-55 0.41111973177246597 1103 56-57 0.441453210977798 1103 58-59 0.43071542704856114 1103 60-61 0.49404969192681847 1103 62-63 0.39016709992569787 1103 64-65 0.4565002609545701 1103 66-67 0.39785168999395637 1103 68-69 0.38758000828482864 1103 70-71 0.35120733837279516 1103 72-73 0.4158215710326729 1103 74-75 0.32757095282762094 1103 76-77 0.32172972961704716 1103 78-79 0.37792306405050624 1103 80-81 0.38621610829204656 1103 82-83 0.26517422728913687 1103 84-85 0.39352669771120574 1103 86-87 0.45120990370968883 1103 88-89 0.5052879388327369 1103 90-91 0.5177366275188042 1103 92-93 0.3971067248914082 1103 94-95 0.46182792521271665 1103 96-97 0.4678976951195857 1103 98-99 0.5048627699532346 1103 100-101 0.7085504278999792 1104 1 0.09849119613476987 1104 2 0.15232180512205673 1104 3 0.1575278606172681 1104 4 0.17112117289887152 1104 5 0.1967963870226086 1104 6 0.23036478240462088 1104 7 0.10168710385484303 1104 8 0.07725246335276381 1104 9 0.21810886652523465 1104 10-11 0.20807671837381037 1104 12-13 0.20556559790588125 1104 14-15 0.1729524436442773 1104 16-17 0.19251747875838987 1104 18-19 0.2008767614328164 1104 20-21 0.2394171258276927 1104 22-23 0.23844238118255134 1104 24-25 0.21375667926469788 1104 26-27 0.2037515416551514 1104 28-29 0.21837226960657574 1104 30-31 0.16907007909745175 1104 32-33 0.16940564238654332 1104 34-35 0.2048895457494524 1104 36-37 0.24882449499745718 1104 38-39 0.2303780893815386 1104 40-41 0.274583784113851 1104 42-43 0.1425296485404388 1104 44-45 0.20000842862486934 1104 46-47 0.17075220612540676 1104 48-49 0.0387096539865297 1104 50-51 0.10681490771678881 1104 52-53 0.10759638121388804 1104 54-55 0.1264233630080156 1104 56-57 0.16066146217914934 1104 58-59 0.2548233216283009 1104 60-61 0.28988509724536016 1104 62-63 0.208656285265711 1104 64-65 0.30967750751340617 1104 66-67 0.2819764408043497 1104 68-69 0.3198763197639707 1104 70-71 0.1555149551717463 1104 72-73 0.27423743823897695 1104 74-75 0.3107221034006358 1104 76-77 0.2855060663746585 1104 78-79 0.30305237504178706 1104 80-81 0.3478930915090359 1104 82-83 0.2507705164481884 1104 84-85 0.2657485294706099 1104 86-87 0.38200825443397335 1104 88-89 0.34212593404700087 1104 90-91 0.523052603914202 1104 92-93 0.515159977288171 1104 94-95 0.48831233658120254 1104 96-97 0.5703076332379275 1104 98-99 0.6644828342786369 1104 100-101 0.7154697017881446 1105 1 0.1037643697950763 1105 2 0.12243188781096137 1105 3 0.07817540630903252 1105 4 0.07999210741431995 1105 5 0.10499132984140402 1105 6 0.14294455142022855 1105 7 0.09912107276220183 1105 8 0.11948090835714709 1105 9 0.10818339283219558 1105 10-11 0.11141347499302157 1105 12-13 0.09830688096301543 1105 14-15 0.13498919906623996 1105 16-17 0.09750434873049585 1105 18-19 0.15594679471219308 1105 20-21 0.1522133959880776 1105 22-23 0.11243504732044585 1105 24-25 0.1395805372945773 1105 26-27 0.12097331892453411 1105 28-29 0.0660968140492102 1105 30-31 0.09578766655607751 1105 32-33 0.09664061516672717 1105 34-35 0.10242880163527701 1105 36-37 0.20768808306009134 1105 38-39 0.17400938086377238 1105 40-41 0.19680908874854453 1105 42-43 0.14498693021013054 1105 44-45 0.08345341361410163 1105 46-47 0.12006440783033412 1105 48-49 0.19917772533019473 1105 50-51 0.15713146282735835 1105 52-53 0.06033225757403926 1105 54-55 0.19941111646555498 1105 56-57 0.08614055123474174 1105 58-59 0.021408824137196802 1105 60-61 -0.08002317720440288 1105 62-63 0.06607307460277667 1105 64-65 0.08059719234776708 1105 66-67 0.11884294766271353 1105 68-69 0.05985397631747702 1105 70-71 -0.007488611989195704 1105 72-73 0.015928470828171726 1105 74-75 0.09179270865784872 1105 76-77 0.10318215739175685 1105 78-79 0.1285372620056222 1105 80-81 0.1451585079447142 1105 82-83 0.2467786861308987 1105 84-85 0.2788109244625687 1105 86-87 0.23020898335722606 1105 88-89 0.2643258464853808 1105 90-91 0.2463885916009403 1105 92-93 0.20041445768289634 1105 94-95 0.21384614743584152 1105 96-97 0.26189533771536944 1105 98-99 0.3626625608905414 1105 100-101 0.5030488735882876 1106 1 -0.08076693483826247 1106 2 0.027852950898548556 1106 3 -0.08907248289852276 1106 4 0.03965546312419832 1106 5 0.013420567324473609 1106 6 0.09083686065021368 1106 7 0.11598034978852212 1106 8 0.028687863778188216 1106 9 -0.04426011997603041 1106 10-11 0.0067284114962191666 1106 12-13 -0.036492415534844724 1106 14-15 0.1438760399284149 1106 16-17 0.09158332170758143 1106 18-19 -0.01996921971501564 1106 20-21 0.07780694576398872 1106 22-23 0.02765898252384602 1106 24-25 0.18573757248976364 1106 26-27 0.25884886677931007 1106 28-29 0.2210938649356109 1106 30-31 0.1486943747294447 1106 32-33 0.14781864526877797 1106 34-35 0.0882478587647384 1106 36-37 0.03120371364261132 1106 38-39 0.09703657642500474 1106 40-41 0.03359267372872665 1106 42-43 0.0267256800260256 1106 44-45 -0.004407974793579683 1106 46-47 0.041820197779408375 1106 48-49 0.12284361039015579 1106 50-51 0.07622798858307789 1106 52-53 -0.10160697475676983 1106 54-55 -0.17227281337806488 1106 56-57 -0.02115067502329282 1106 58-59 0.04788781750792026 1106 60-61 0.15475450573458716 1106 62-63 0.015906046971636556 1106 64-65 -0.10573724734251755 1106 66-67 -0.09119147305857922 1106 68-69 -0.09545315776668417 1106 70-71 -0.13195347519081935 1106 72-73 -0.14094720973110952 1106 74-75 -0.13180767669000204 1106 76-77 -0.181681716168562 1106 78-79 -0.10319207341852632 1106 80-81 0.046749228859962244 1106 82-83 0.011764395437591446 1106 84-85 0.15386639561796045 1106 86-87 0.368001787205948 1106 88-89 0.16894434459571528 1106 90-91 0.24148207255672105 1106 92-93 0.13542681614030627 1106 94-95 0.28619291552031 1106 96-97 0.2787853089679615 1106 98-99 0.4511981566019543 1106 100-101 0.346470586288536 1107 1 0.07665147245699089 1107 2 0.08696348061648962 1107 3 0.05866395958240389 1107 4 0.09816613638324867 1107 5 0.04686873725887608 1107 6 0.03275583595496556 1107 7 0.14573592885602693 1107 8 0.04565005598569627 1107 9 -0.006726222522985381 1107 10-11 -0.05201596863539493 1107 12-13 -0.05548460460361326 1107 14-15 0.039463480666185546 1107 16-17 0.10324919788624243 1107 18-19 0.007470282887467761 1107 20-21 -0.052256631194687486 1107 22-23 -0.06915377960090296 1107 24-25 -0.026287135575081777 1107 26-27 -0.03225372890849343 1107 28-29 0.04246494014876134 1107 30-31 0.031717178103477295 1107 32-33 0.04790949336724992 1107 34-35 0.024655352590990276 1107 36-37 0.13009214733172314 1107 38-39 0.07062755361021544 1107 40-41 0.09446222259912673 1107 42-43 0.00688546629069009 1107 44-45 0.010355238076442674 1107 46-47 0.10126978557914157 1107 48-49 0.05671772478132908 1107 50-51 0.1576578797241055 1107 52-53 0.12126548225538869 1107 54-55 0.0538007510228411 1107 56-57 0.17431842228025118 1107 58-59 0.15178925177714575 1107 60-61 0.31087618314363397 1107 62-63 0.28672788388008286 1107 64-65 0.2186843967295573 1107 66-67 0.09393339712361382 1107 68-69 0.20492150708821555 1107 70-71 0.258608606339088 1107 72-73 0.19841874462370868 1107 74-75 0.19108928869943753 1107 76-77 0.1267086293132209 1107 78-79 0.19831954341304936 1107 80-81 0.3262705022706882 1107 82-83 0.353916715559329 1107 84-85 0.3183690677273425 1107 86-87 0.3319078335061718 1107 88-89 0.404966618254587 1107 90-91 0.3692122779341389 1107 92-93 0.3885029308049788 1107 94-95 0.3719886468294007 1107 96-97 0.39581881916080164 1107 98-99 0.37529705598085883 1107 100-101 0.5989759621175388 1108 1 -0.014687114517101918 1108 2 0.007332295966108404 1108 3 0.010339814413185877 1108 4 0.031119214467111078 1108 5 -0.07758351659830254 1108 6 -0.029251481742782914 1108 7 0.05271896996394787 1108 8 -0.06521977704478843 1108 9 -0.13206470935929815 1108 10-11 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-0.9661273315320003 2316 92-93 -0.9349665785968888 2316 94-95 -0.7988150079976748 2316 96-97 -0.9437637536981249 2316 98-99 -1.1868999809868015 2316 100-101 -1.4897337055329238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136.0 3 75.0 4 233.0 5 622.0 6 1165.0 7 2106.0 8 3586.0 9 5410.0 10 7593.0 11 9669.0 12 11938.0 13 14574.0 14 16626.0 15 19203.0 16 22276.0 17 26137.0 18 30043.0 19 34648.0 20 37095.0 21 39347.0 22 44310.0 23 52830.0 24 64978.0 25 81651.0 26 102953.0 27 131763.0 28 165024.0 29 204192.0 30 249178.0 31 301401.0 32 367497.0 33 454430.0 34 583749.0 35 820348.0 36 1055234.0 37 1484882.0 38 1324888.0 39 266144.0 40 316.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.76537961796565 31.11020051078615 27.66419429957275 22.46022557167545 2 23.189313594376888 28.96326936833266 27.727913413989363 20.119503623301092 3 21.42553416477467 28.14233197524337 28.284850558268282 22.14728330171368 4 21.990113973455127 28.578459449923688 27.907373116636304 21.52405345998488 5 22.612229745860436 28.391282100852912 27.818842004966026 21.17764614832063 6 22.318865244627105 28.28000840489499 27.56725937598009 21.833866974497813 7 22.48157248157248 28.51208907411439 27.59146580412403 21.414872640189095 8 21.726901919862236 28.355137254667785 27.899415207297423 22.01854561817255 9 21.527917302120134 28.21670909105159 28.28937440526345 21.965999201564824 10-11 21.621415176089155 28.681060731738224 27.763559290036437 21.933964802136188 12-13 21.59472218225704 28.76375463230693 27.586171934388926 22.055351251047103 14-15 21.767393400304794 28.75760271203309 27.438067987435076 22.03693590022704 16-17 21.95175703366759 28.574088176785256 27.372296667327877 22.101858122219273 18-19 21.919266486926006 28.63655736921777 27.35993589647893 22.08424024737729 20-21 22.020564693055437 28.54239415255299 27.36683874821898 22.070202406172594 22-23 21.948763264181142 28.535618478008207 27.426021864372863 22.089596393437787 24-25 21.971355705532922 28.47361054955992 27.35229061051846 22.202743134388705 26-27 22.116990638509627 28.411072061704974 27.44193698877243 22.03000031101297 28-29 22.117765484106688 28.478387296174013 27.350072883472727 22.05377433624658 30-31 22.091628959487117 28.475706433068865 27.339915986850947 22.09274862059307 32-33 22.11674912888128 28.492962440597907 27.259755167401966 22.130533263118846 34-35 22.11469727569649 28.55808698373947 27.236074622323947 22.091141118240092 36-37 22.106614986977235 28.576694477093966 27.187377771629386 22.129312764299414 38-39 22.155250741633864 28.52019263850597 27.213918053058006 22.11063856680216 40-41 22.12513359949351 28.629355971752734 27.154483500097097 22.091026928656657 42-43 22.1331733144303 28.605367334571834 27.125841464593453 22.13561788640441 44-45 22.142530000280537 28.62768127730822 27.13010404733438 22.099684675076865 46-47 22.153223173727312 28.650231307304296 27.102884722570575 22.093660796397817 48-49 22.163457100265223 28.66071093100525 27.06500158875465 22.110830379974875 50-51 22.190608726506746 28.634020164831846 27.068405645316858 22.10696546334455 52-53 22.14280053354587 28.69962066784804 27.021502953575265 22.136075845030827 54-55 22.16557252689525 28.72585150532213 26.969291409849404 22.139284557933216 56-57 22.1612042422169 28.74583398127702 26.93104842471931 22.16191335178677 58-59 22.14066076844864 28.827243054362473 26.910245480140134 22.121850697048746 60-61 22.150629200071037 28.80783062589087 26.904228661143236 22.13731151289486 62-63 22.117380317391202 28.86545007814262 26.903061168951336 22.114108435514847 64-65 22.127212243506648 28.904682877552496 26.889858700060458 22.0782461788804 66-67 22.098806102056578 28.954084044602972 26.847215200203827 22.09989465313662 68-69 22.089473268759498 28.986101781514577 26.865412524227317 22.059012425498604 70-71 22.117304662769342 29.052888079029998 26.78792405677765 22.041883201423012 72-73 22.063544630817333 29.00687441396047 26.80797510147651 22.12160585374568 74-75 22.152303368151628 29.02625548209943 26.779118776115123 22.042322373633823 76-77 22.064740459677168 29.14776226168631 26.766317295431218 22.0211799832053 78-79 22.104189344695673 29.116804030728083 26.75439305819053 22.024613566385717 80-81 22.044893519945436 29.151521616821093 26.728523057123493 22.07506180610998 82-83 22.058811546241902 29.224483311602818 26.689778707003388 22.026926435151896 84-85 22.044113780429328 29.24139517417782 26.681567530390527 22.032923515002327 86-87 22.02224271918822 29.26478649164237 26.675942138176367 22.03702865099304 88-89 22.009026333492884 29.34969780039493 26.65097897245603 21.990296893656154 90-91 21.99393471703329 29.341419050293183 26.634521852945564 22.030124379727965 92-93 22.009594794862664 29.379324072360614 26.59887381678451 22.012207315992207 94-95 22.00464044974083 29.468697055738456 26.57631882742338 21.95034366709734 96-97 21.9709500323114 29.462106772990843 26.548339366930428 22.01860382776733 98-99 21.933480546138775 29.51214505644885 26.53029577333375 22.024078624078626 100-101 22.0332638385091 29.511690264365427 26.441730434186006 22.01331546293947 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 137115.0 1 77423.0 2 13302.0 3 8076.5 4 6891.5 5 6099.5 6 5640.0 7 5561.5 8 5250.5 9 4850.0 10 4669.0 11 5073.5 12 8267.5 13 8110.5 14 5281.0 15 5558.5 16 5983.5 17 6645.5 18 7885.5 19 9634.0 20 12285.0 21 15731.0 22 19620.5 23 24510.5 24 30414.5 25 37662.0 26 48185.0 27 59961.0 28 68480.5 29 76353.5 30 88610.5 31 103732.0 32 120045.0 33 138732.5 34 156798.0 35 172188.0 36 188347.0 37 204771.5 38 217081.0 39 222972.0 40 230879.0 41 242583.5 42 251453.0 43 262171.0 44 273425.5 45 283278.0 46 291240.0 47 296955.5 48 305644.0 49 311344.5 50 310386.5 51 305022.5 52 296780.0 53 288294.5 54 275177.5 55 256731.0 56 233737.5 57 210533.5 58 190986.5 59 165686.0 60 135044.0 61 105100.0 62 78182.5 63 57060.0 64 40817.0 65 26771.5 66 15984.5 67 10121.5 68 5834.5 69 2777.5 70 1366.5 71 680.5 72 346.0 73 196.0 74 118.0 75 74.5 76 51.5 77 34.5 78 27.5 79 24.5 80 19.0 81 15.5 82 13.0 83 8.0 84 6.5 85 5.5 86 4.5 87 5.0 88 2.0 89 1.0 90 2.0 91 2.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14678568096289615 2 0.0 3 0.0 4 0.002960843467172581 5 3.6077504431934813E-4 6 9.579199452617173E-4 7 0.0 8 2.114888190837558E-4 9 3.3589400678008273E-4 10-11 8.08633720026125E-5 12-13 4.3541815693714425E-5 14-15 0.0 16-17 6.220259384816347E-6 18-19 2.488103753926539E-5 20-21 8.708363138742885E-5 22-23 1.6794700339004136E-4 24-25 0.0 26-27 0.0 28-29 0.005218797623860915 30-31 0.0012875936926569837 32-33 2.8613193170155197E-4 34-35 1.4928622523559232E-4 36-37 2.2392933785338846E-4 38-39 0.0010636643548035953 40-41 0.0028986408733244176 42-43 4.1053711939787883E-4 44-45 4.167573787826953E-4 46-47 0.005697757596491774 48-49 0.006319783534973408 50-51 0.006761421951295369 52-53 0.008745684695051784 54-55 0.004385282866295524 56-57 0.0 58-59 3.732155630889808E-5 60-61 8.397350169502068E-4 62-63 7.775324231020434E-4 64-65 3.732155630889808E-4 66-67 5.225017883245731E-4 68-69 7.650919043324108E-4 70-71 0.0010823251329580444 72-73 0.0022766149348427828 74-75 5.349423070942058E-4 76-77 0.0 78-79 0.0 80-81 9.33038907722452E-5 82-83 1.9282804092930677E-4 84-85 1.6794700339004136E-4 86-87 0.0025378658290050693 88-89 0.0012751531738873511 90-91 5.349423070942058E-4 92-93 4.6651945386122604E-4 94-95 2.5503063477747023E-4 96-97 8.148539794109414E-4 98-99 0.0 100-101 1.866077815444904E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8038250.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.89465692166448 #Duplication Level Percentage of deduplicated Percentage of total 1 90.73158669812791 58.87995190733268 2 5.188496139015358 6.734113537615649 3 1.2720927263266306 2.476560631425346 4 0.6170998979761083 1.6018594466221483 5 0.3953723053458158 1.2828775055872144 6 0.22523714158697397 0.8770012217581826 7 0.16236076862615462 0.7375442464272436 8 0.13007218337371676 0.6752791772071345 9 0.105519943127093 0.6162912456863624 >10 1.088377820929325 15.10956085292921 >50 0.07697811877489145 3.2751166339169724 >100 0.0064026991317123845 0.5506661977205421 >500 0.0 0.0 >1k 1.345192194594955E-4 0.27995758557994327 >5k 1.345192194594955E-4 0.6036503599268187 >10k+ 1.345192194594955E-4 6.299569450264496 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122060 1.5184897210213666 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22267 0.2770130314434112 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8760 0.1089789444219824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 1.2440518769632694E-5 0.0 1.2440518769632694E-5 0.0 2 0.0 1.2440518769632694E-5 0.0 1.2440518769632694E-5 0.0 3 0.0 1.2440518769632694E-5 0.0 1.2440518769632694E-5 0.0 4 0.0 2.488103753926539E-5 0.0 2.488103753926539E-5 0.0 5 0.0 2.488103753926539E-5 0.0 3.732155630889808E-5 0.0 6 0.0 2.488103753926539E-5 0.0 3.732155630889808E-5 0.0 7 0.0 2.488103753926539E-5 0.0 3.732155630889808E-5 0.0 8 1.2440518769632694E-5 2.488103753926539E-5 0.0 3.732155630889808E-5 0.0 9 1.2440518769632694E-5 2.488103753926539E-5 0.0 3.732155630889808E-5 0.0 10-11 1.2440518769632694E-5 3.732155630889808E-5 0.0 3.732155630889808E-5 0.0 12-13 1.2440518769632694E-5 4.354181569371443E-5 0.0 3.732155630889808E-5 0.0 14-15 1.2440518769632694E-5 4.976207507853078E-5 0.0 4.354181569371443E-5 0.0 16-17 1.2440518769632694E-5 6.220259384816347E-5 0.0 4.976207507853078E-5 0.0 18-19 1.2440518769632694E-5 8.08633720026125E-5 0.0 4.976207507853078E-5 0.0 20-21 1.2440518769632694E-5 8.708363138742885E-5 0.0 4.976207507853078E-5 0.0 22-23 1.866077815444904E-5 8.708363138742885E-5 0.0 4.976207507853078E-5 0.0 24-25 2.488103753926539E-5 8.708363138742885E-5 0.0 4.976207507853078E-5 0.0 26-27 2.488103753926539E-5 8.708363138742885E-5 0.0 4.976207507853078E-5 0.0 28-29 2.488103753926539E-5 9.33038907722452E-5 0.0 5.598233446334712E-5 0.0 30-31 2.488103753926539E-5 1.057444095418779E-4 0.0 6.220259384816347E-5 0.0 32-33 2.488103753926539E-5 1.1196466892669424E-4 0.0 6.220259384816347E-5 0.0 34-35 2.488103753926539E-5 1.181849283115106E-4 0.0 6.220259384816347E-5 0.0 36-37 3.732155630889808E-5 1.2440518769632695E-4 0.0 6.220259384816347E-5 0.0 38-39 6.842285323297982E-5 1.2440518769632695E-4 0.0 6.220259384816347E-5 0.0 40-41 9.952415015706155E-5 1.2440518769632695E-4 0.0 6.220259384816347E-5 0.0 42-43 9.952415015706155E-5 1.3684570646595962E-4 0.0 6.220259384816347E-5 0.0 44-45 1.3062544708114328E-4 1.3684570646595962E-4 0.0 6.220259384816347E-5 0.0 46-47 1.741672627748577E-4 1.3684570646595962E-4 0.0 6.842285323297982E-5 0.0 48-49 1.8038752215967407E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 50-51 2.0526855969893944E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 52-53 2.2392933785338848E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 54-55 2.736914129319193E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 56-57 3.3589400678008273E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 58-59 3.732155630889808E-4 1.3684570646595962E-4 0.0 7.464311261779616E-5 0.0 60-61 4.789599726308587E-4 1.4306596585077599E-4 0.0 7.464311261779616E-5 0.0 62-63 5.660436040182875E-4 1.6172674400522503E-4 0.0 7.464311261779616E-5 0.0 64-65 6.469069760209E-4 1.6172674400522503E-4 0.0 7.464311261779616E-5 0.0 66-67 7.961932012564923E-4 1.6172674400522503E-4 0.0 7.464311261779616E-5 0.0 68-69 9.454794264920848E-4 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 70-71 0.0010761048735732281 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 72-73 0.0012627126551177184 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 74-75 0.0014742014742014742 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 76-77 0.0017727739246726588 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 78-79 0.002027804559450129 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 80-81 0.0024881037539265386 1.6172674400522503E-4 0.0 1.1196466892669424E-4 0.0 82-83 0.0029421826890181323 1.741672627748577E-4 0.0 1.1196466892669424E-4 6.220259384816347E-6 84-85 0.0035455478493453176 1.741672627748577E-4 0.0 1.2440518769632695E-4 1.2440518769632694E-5 86-87 0.004491027275837403 1.741672627748577E-4 0.0 1.2440518769632695E-4 1.2440518769632694E-5 88-89 0.005915466674960346 1.866077815444904E-4 0.0 1.2440518769632695E-4 1.2440518769632694E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 375 0.0 27.865831 3 CGCGTAG 220 0.0 24.829496 54-55 CTCGCGT 230 0.0 23.75106 52-53 CGTACGC 285 0.0 19.998777 20-21 CAACGCA 5910 0.0 19.85073 12-13 ATCAACG 6090 0.0 19.57598 10-11 ACGTACG 340 0.0 18.160656 20-21 TCAACGC 5720 0.0 18.102041 10-11 ACTCGCG 305 0.0 17.911083 50-51 CGTAGCG 310 0.0 16.854334 56-57 AACGCAG 6155 0.0 16.70694 12-13 GGTATCA 7215 0.0 16.655357 8 GTATCAA 7595 0.0 16.009655 9 AGTGGTA 7580 0.0 15.916113 5 TCGCGTA 345 0.0 15.83404 52-53 ACGCAGA 7555 0.0 15.654238 14-15 TGGTATC 8500 0.0 14.584495 7 TATCAAC 8235 0.0 14.36169 9 CGCTAGT 390 0.0 14.00586 90-91 GTGGTAT 8975 0.0 13.6009245 6 >>END_MODULE