FastQCFastQC Report
Sat 18 Jun 2016
SRR578567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920275
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109870213.872018332696781No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT257230.3247740766576918No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG92030.11619546038489825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83560.10550138726243723No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT79210.10000915372256645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2383950.091.885465
GGTATCA2396600.091.757018
GTATCAA2402600.091.66639
TGGTATC2395400.091.5242847
GTGGTAT2401950.091.392336
AAGCAGT2415450.091.3080751
CAGTGGT2418200.090.61774
GCAGTGG2427400.090.557553
AGCAGTG2432150.090.542862
TCAACGC2396050.046.51070812-13
AACGCAG2407100.046.35622814-15
CAACGCA2396200.046.3263812-13
ATCAACG2396150.046.30093810-11
CGCAGAG2413350.046.19909316-17
ACGCAGA2409050.046.11360514-15
TATCAAC2411250.046.11147710-11
CAGAGTA2394950.045.9564418-19
AGAGTAC2352050.045.86060318-19
GAGTACT1386500.045.72399520-21
GCAGAGT2427700.045.50337616-17
Produced by FastQC (version 0.11.5)