##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7920275 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.638599038543486 31.0 31.0 33.0 27.0 34.0 2 30.93563796711604 31.0 31.0 34.0 27.0 34.0 3 31.039470599190054 31.0 31.0 34.0 27.0 34.0 4 34.48361363715275 37.0 35.0 37.0 30.0 37.0 5 34.5232415541127 37.0 35.0 37.0 30.0 37.0 6 34.525837297316066 37.0 35.0 37.0 30.0 37.0 7 34.41745621206334 37.0 35.0 37.0 30.0 37.0 8 34.390907260164575 37.0 35.0 37.0 30.0 37.0 9 35.82066923181329 39.0 35.0 39.0 30.0 39.0 10-11 35.777335067279864 39.0 35.0 39.0 30.0 39.0 12-13 35.85578417416062 39.0 35.0 39.0 30.0 39.0 14-15 36.89947665958569 40.0 36.0 41.0 31.0 41.0 16-17 36.86411683937742 40.0 36.0 41.0 30.5 41.0 18-19 36.837415809425806 40.0 36.0 41.0 30.0 41.0 20-21 36.712973223783266 39.5 36.0 41.0 30.0 41.0 22-23 36.39116735214371 39.0 36.0 40.5 29.0 41.0 24-25 36.29071982980389 39.0 36.0 40.0 29.0 41.0 26-27 36.24037624956204 39.0 36.0 40.0 29.0 41.0 28-29 35.944381666040634 39.0 35.0 41.0 27.0 41.0 30-31 35.66016609271774 38.5 34.5 40.5 26.5 41.0 32-33 35.28604859048455 38.0 34.0 40.0 25.0 41.0 34-35 35.08687734958698 38.0 34.0 40.0 25.0 41.0 36-37 35.09269210980679 38.0 34.0 40.0 25.0 41.0 38-39 34.33682100684635 37.0 33.5 40.0 25.0 41.0 40-41 34.29312568061084 37.0 33.0 40.0 24.5 41.0 42-43 34.10002335777482 37.0 33.0 40.0 23.5 41.0 44-45 33.93837505642165 37.0 33.0 40.0 23.0 41.0 46-47 33.721867864436526 37.0 33.0 40.0 23.0 41.0 48-49 33.3311273282809 36.0 33.0 40.0 21.0 41.0 50-51 31.986649390330513 35.5 31.0 39.0 14.5 40.5 52-53 31.513524277881764 35.5 31.0 39.0 5.0 40.0 54-55 31.09312568061084 36.0 29.5 40.0 2.0 41.0 56-57 30.526829813358752 36.0 28.5 40.0 2.0 41.0 58-59 30.186525215349214 36.0 28.0 40.0 2.0 41.0 60-61 29.875890546729757 35.0 28.0 39.0 2.0 41.0 62-63 29.526951968208174 35.0 27.0 39.0 2.0 41.0 64-65 29.183892604739103 35.0 26.5 39.0 2.0 40.5 66-67 28.862086669970424 35.0 26.0 38.0 2.0 40.0 68-69 28.514637812449692 35.0 26.0 37.5 2.0 40.0 70-71 28.120383825056578 34.5 26.0 37.0 2.0 39.5 72-73 27.732534728907773 34.0 25.0 36.5 2.0 39.0 74-75 27.340582113626105 34.0 24.5 36.0 2.0 39.0 76-77 26.938694110999933 34.0 23.5 35.5 2.0 38.5 78-79 26.551730968937314 34.0 22.0 35.0 2.0 37.0 80-81 26.170020990432782 33.0 20.0 35.0 2.0 37.0 82-83 25.803510547297915 33.0 19.5 35.0 2.0 36.0 84-85 25.39416598539823 33.0 18.0 35.0 2.0 36.0 86-87 25.04048394784272 32.5 15.0 35.0 2.0 35.5 88-89 24.660246961121928 32.0 8.5 35.0 2.0 35.0 90-91 24.282466833537974 32.0 4.5 35.0 2.0 35.0 92-93 23.90732304118228 32.0 2.0 35.0 2.0 35.0 94-95 23.46501150528233 31.0 2.0 35.0 2.0 35.0 96-97 22.982765308022763 31.0 2.0 34.5 2.0 35.0 98-99 22.377512838885014 31.0 2.0 34.0 2.0 35.0 100-101 20.827394377089178 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.527682479885172 1101 2 0.5561208382022471 1101 3 0.6234779555016914 1101 4 0.6838017138518637 1101 5 0.685733912892978 1101 6 0.5733932786702169 1101 7 0.5811594372416025 1101 8 0.7167248320982793 1101 9 0.6990183866541884 1101 10-11 0.7341938296669568 1101 12-13 0.6910630161723077 1101 14-15 0.8288037923069069 1101 16-17 0.8429934934519423 1101 18-19 0.7522137838959893 1101 20-21 0.781820941432926 1101 22-23 0.8626091435540602 1101 24-25 1.021976271576996 1101 26-27 0.912030834933482 1101 28-29 0.8786702446438639 1101 30-31 1.0507081103816986 1101 32-33 1.176759528595852 1101 34-35 1.1043305259065193 1101 36-37 0.9868734529029126 1101 38-39 1.0682793501977343 1101 40-41 1.061560142910679 1101 42-43 1.2716149669902777 1101 44-45 1.23752174345843 1101 46-47 1.1875793189032038 1101 48-49 1.1918031527865125 1101 50-51 1.2225321223846635 1101 52-53 1.25725283304104 1101 54-55 1.1186415658361426 1101 56-57 1.108041326011282 1101 58-59 1.2289863063311905 1101 60-61 1.201857224272441 1101 62-63 1.1974610208770038 1101 64-65 1.1706687658223984 1101 66-67 1.1124789943620712 1101 68-69 1.2485593928648484 1101 70-71 1.1328867952732686 1101 72-73 1.2264821865500117 1101 74-75 1.3073250522206692 1101 76-77 1.1781463649925286 1101 78-79 1.1660472821103802 1101 80-81 1.2263437664059076 1101 82-83 1.3044978729433545 1101 84-85 1.391099583919015 1101 86-87 1.4358955852189297 1101 88-89 1.536732347438324 1101 90-91 1.727866892321817 1101 92-93 1.655149103529297 1101 94-95 1.7948517886434452 1101 96-97 2.10010494759079 1101 98-99 2.3154469950062406 1101 100-101 2.713121417568832 1102 1 0.5453220923717161 1102 2 0.5237523728916962 1102 3 0.5133263174017237 1102 4 0.6101232543374522 1102 5 0.5103229775061564 1102 6 0.4353486355009224 1102 7 0.5429098351513062 1102 8 0.5844211290079215 1102 9 0.5511899156394975 1102 10-11 0.6912739949029927 1102 12-13 0.6450238121757295 1102 14-15 0.7986496002246284 1102 16-17 0.8553750230812582 1102 18-19 0.7418695740572687 1102 20-21 0.7418363462888067 1102 22-23 0.8036705844932257 1102 24-25 0.9371893138152245 1102 26-27 0.9625741597106057 1102 28-29 0.8796455199605759 1102 30-31 0.9787427841877374 1102 32-33 1.0036980950168726 1102 34-35 1.0091270077406804 1102 36-37 0.9495756692369923 1102 38-39 1.0276977856075575 1102 40-41 1.0151684304728974 1102 42-43 1.0749459045851495 1102 44-45 1.018135413151981 1102 46-47 1.0179444610926751 1102 48-49 1.1980872593987115 1102 50-51 1.1434012859922547 1102 52-53 1.1861179927109369 1102 54-55 1.1704357310537787 1102 56-57 1.069996616600374 1102 58-59 1.0969340564162984 1102 60-61 1.1195850190706373 1102 62-63 1.0619150703558056 1102 64-65 1.1303700970948825 1102 66-67 1.121065107543231 1102 68-69 1.2844125940829585 1102 70-71 1.1946839082428262 1102 72-73 1.2221216628732314 1102 74-75 1.344121983025783 1102 76-77 1.2234648970047104 1102 78-79 1.2355992495783852 1102 80-81 1.316918960760649 1102 82-83 1.474438185566985 1102 84-85 1.4343113381258163 1102 86-87 1.4671181662499073 1102 88-89 1.6358086301796213 1102 90-91 1.614263226952385 1102 92-93 1.5097057281509372 1102 94-95 1.6649348593103674 1102 96-97 1.8683824484418103 1102 98-99 1.9197949916573585 1102 100-101 2.2137062647991073 1103 1 0.3823503297472648 1103 2 0.3364478060179792 1103 3 0.26390995712520393 1103 4 0.3791735330148285 1103 5 0.34672610712398466 1103 6 0.23125122511279272 1103 7 0.48857006570655415 1103 8 0.3279344993706843 1103 9 0.5486860280426598 1103 10-11 0.4651407758973676 1103 12-13 0.36080666003294226 1103 14-15 0.5759412428405497 1103 16-17 0.5734816943983745 1103 18-19 0.5656880355587006 1103 20-21 0.7154666063807582 1103 22-23 0.6720388741065122 1103 24-25 0.5452163649288977 1103 26-27 0.7851443276046197 1103 28-29 0.6560629812752623 1103 30-31 0.5987703868029612 1103 32-33 0.8430494028659581 1103 34-35 0.8702566745869618 1103 36-37 0.8379015968419594 1103 38-39 0.49253269177162196 1103 40-41 0.6295241526148558 1103 42-43 0.5107938982401024 1103 44-45 0.587690813721423 1103 46-47 0.7027084304919384 1103 48-49 0.7175205105217088 1103 50-51 0.6710397648800779 1103 52-53 0.5654166896185622 1103 54-55 0.5705023940114486 1103 56-57 0.48742894489198463 1103 58-59 0.5785552013287507 1103 60-61 0.5893483715230836 1103 62-63 0.535171648419503 1103 64-65 0.5121886233961703 1103 66-67 0.49564271626194767 1103 68-69 0.6868097641508335 1103 70-71 0.6893453855856997 1103 72-73 0.682250526232874 1103 74-75 0.8660139855921294 1103 76-77 0.9078965914539197 1103 78-79 0.9462098915359682 1103 80-81 0.9571197347474332 1103 82-83 1.0726443727081296 1103 84-85 1.0349752081868182 1103 86-87 1.1892050947524488 1103 88-89 1.2329888769982205 1103 90-91 1.0477067845796988 1103 92-93 1.1598233109144296 1103 94-95 1.241919789787282 1103 96-97 1.3967296452579134 1103 98-99 1.4933419944066486 1103 100-101 1.8163231591537858 1104 1 0.1608577464096932 1104 2 0.2979687123844492 1104 3 0.22434118514104284 1104 4 0.2957700625843884 1104 5 0.15035861356544444 1104 6 0.1943589557791796 1104 7 0.4000757713221148 1104 8 0.33401839359482466 1104 9 0.4293116091862146 1104 10-11 0.44461076331374016 1104 12-13 0.308056233237167 1104 14-15 0.42078171223109706 1104 16-17 0.5351142377159661 1104 18-19 0.659755285180772 1104 20-21 0.4986834858536753 1104 22-23 0.39749703377329837 1104 24-25 0.4393438877087945 1104 26-27 0.4992068887304342 1104 28-29 0.6140166189991945 1104 30-31 0.5049552045810302 1104 32-33 0.6778414741792886 1104 34-35 0.6822237338594306 1104 36-37 0.7373320026450472 1104 38-39 0.553039578360945 1104 40-41 0.6305030036983155 1104 42-43 0.71963668258811 1104 44-45 0.9105896930875801 1104 46-47 0.7645900965169687 1104 48-49 0.7265118875012107 1104 50-51 0.8312167578155751 1104 52-53 0.7037720464975656 1104 54-55 0.8495610562959364 1104 56-57 0.9151462847341527 1104 58-59 1.0107613651032672 1104 60-61 0.9738290355457444 1104 62-63 0.8494281814113265 1104 64-65 0.9756417908073622 1104 66-67 0.8285352406033901 1104 68-69 0.9374849482381649 1104 70-71 1.124948457657318 1104 72-73 0.8427836025469482 1104 74-75 0.9370574670695042 1104 76-77 0.9496793447822256 1104 78-79 1.057367822266741 1104 80-81 0.9596987059936843 1104 82-83 0.9429438554848595 1104 84-85 0.9141103032163009 1104 86-87 1.0071043035321274 1104 88-89 1.095295436753652 1104 90-91 1.2441206929159172 1104 92-93 1.2137435889775112 1104 94-95 1.2766239024334674 1104 96-97 1.5181457035181118 1104 98-99 1.6714265259230814 1104 100-101 2.0059759507636663 1105 1 -0.01496036353461605 1105 2 0.20964344220727327 1105 3 0.12033585840570282 1105 4 0.21004705487010966 1105 5 0.24280959456001483 1105 6 0.205467216451936 1105 7 0.39140779311243534 1105 8 0.373894831359344 1105 9 0.2854814768780827 1105 10-11 0.336016145487541 1105 12-13 0.20708790348480477 1105 14-15 0.14687276444907837 1105 16-17 0.374849962823113 1105 18-19 0.16799027839092417 1105 20-21 0.18715984633206517 1105 22-23 0.33584934897783825 1105 24-25 0.49197514100727346 1105 26-27 0.4684722078737096 1105 28-29 0.5833039991698357 1105 30-31 0.4879124848736822 1105 32-33 0.6452718022971737 1105 34-35 0.48288306414638527 1105 36-37 0.7229456098525873 1105 38-39 0.595377591533726 1105 40-41 0.5499208981016963 1105 42-43 0.7716683623941023 1105 44-45 0.7949766388992074 1105 46-47 0.5914158961588001 1105 48-49 0.7549233330865164 1105 50-51 0.7116008432891192 1105 52-53 0.6420747350583333 1105 54-55 0.8676600170295252 1105 56-57 0.9125701370657389 1105 58-59 0.8927263344107033 1105 60-61 0.8570111590984233 1105 62-63 0.8929645308156715 1105 64-65 0.9884365039440262 1105 66-67 0.9168028619450155 1105 68-69 0.994773425230175 1105 70-71 0.981604308019886 1105 72-73 0.8251502276347651 1105 74-75 0.8260007191090146 1105 76-77 0.7811274630491347 1105 78-79 0.8892283579989488 1105 80-81 0.9465961199121509 1105 82-83 1.0025256125428506 1105 84-85 1.0297609080895604 1105 86-87 1.059454182721332 1105 88-89 0.922921876737071 1105 90-91 1.1007220094429293 1105 92-93 1.2612362017336842 1105 94-95 1.2737504216147002 1105 96-97 1.5860914915943347 1105 98-99 1.8079989642665666 1105 100-101 2.159741738266387 1106 1 0.21314357282107466 1106 2 0.29661325774199554 1106 3 0.19762091517137037 1106 4 0.09047700768440592 1106 5 0.3017635300951156 1106 6 0.24054026298361464 1106 7 0.311043470087462 1106 8 0.28560451967109657 1106 9 0.26436842253857407 1106 10-11 0.20289902032023832 1106 12-13 0.32351329905312554 1106 14-15 0.2030820426401263 1106 16-17 0.327811963192552 1106 18-19 0.2404694895135222 1106 20-21 0.2178566133098201 1106 22-23 0.33786697613708583 1106 24-25 0.5205224188987145 1106 26-27 0.2982293156724225 1106 28-29 0.33833758053104646 1106 30-31 0.4061372261344758 1106 32-33 0.3743118666733807 1106 34-35 0.40305930518491806 1106 36-37 0.5135743821687271 1106 38-39 0.4626409716127924 1106 40-41 0.3712765984653075 1106 42-43 0.5792910642602678 1106 44-45 0.5437262639591438 1106 46-47 0.5900705640448365 1106 48-49 0.7296780812507464 1106 50-51 0.5838006214852243 1106 52-53 0.5962055532639887 1106 54-55 0.7581935571866083 1106 56-57 0.6155754366843276 1106 58-59 0.7419875497019 1106 60-61 0.5967807075745526 1106 62-63 0.6970480428457044 1106 64-65 0.8104002052057453 1106 66-67 0.7966436263373851 1106 68-69 0.767188665204042 1106 70-71 0.8307890294258833 1106 72-73 0.8455510267852624 1106 74-75 0.8260580295063171 1106 76-77 0.8563333701841138 1106 78-79 0.921330451866833 1106 80-81 1.0072381805583355 1106 82-83 1.1163462003580946 1106 84-85 1.1029832774943138 1106 86-87 1.1819389633844466 1106 88-89 1.2572008316258874 1106 90-91 1.075993227733651 1106 92-93 1.1322490261681502 1106 94-95 1.213845735832809 1106 96-97 1.455776705180103 1106 98-99 1.6215360292967986 1106 100-101 1.7925316311042359 1107 1 0.10056081872424727 1107 2 0.20595811993891644 1107 3 0.18518347697408544 1107 4 0.15023286061920516 1107 5 0.24781678217094338 1107 6 0.25708785158196434 1107 7 0.2363920941552422 1107 8 0.24433162775660833 1107 9 0.17053325215677972 1107 10-11 0.2408008140935749 1107 12-13 0.2813444696881149 1107 14-15 0.2776302198670848 1107 16-17 0.1604072735368831 1107 18-19 0.19432062674638217 1107 20-21 0.17567190887039885 1107 22-23 0.41745086134547904 1107 24-25 0.5162021328322979 1107 26-27 0.44587217518832034 1107 28-29 0.5143402776783148 1107 30-31 0.5151736129772573 1107 32-33 0.5007844460097317 1107 34-35 0.5234324607814571 1107 36-37 0.4330678842384472 1107 38-39 0.4144893549169453 1107 40-41 0.37523631091445253 1107 42-43 0.41012926618800805 1107 44-45 0.42258441452969464 1107 46-47 0.5608979597715376 1107 48-49 0.504376158921481 1107 50-51 0.2832593522100595 1107 52-53 0.382038769803664 1107 54-55 0.5408268419097659 1107 56-57 0.3714609579131256 1107 58-59 0.37175572586871297 1107 60-61 0.29965222191284013 1107 62-63 0.417815905256429 1107 64-65 0.4631319088143755 1107 66-67 0.5666263280100168 1107 68-69 0.4927127670979736 1107 70-71 0.5478579137253696 1107 72-73 0.49579450368073097 1107 74-75 0.5784059394316188 1107 76-77 0.6231027595182503 1107 78-79 0.6425909347767309 1107 80-81 0.6513823695205829 1107 82-83 0.6521685666668127 1107 84-85 0.7571928354527557 1107 86-87 0.6995496673331338 1107 88-89 0.8408131402651264 1107 90-91 0.8165570291788242 1107 92-93 0.9158336717218383 1107 94-95 0.9298062954862942 1107 96-97 0.849609997142462 1107 98-99 1.058837462207748 1107 100-101 1.4129422764645376 1108 1 0.1634448663961976 1108 2 0.19325925983249803 1108 3 0.03731021403433843 1108 4 0.140778768444477 1108 5 0.25761969559277276 1108 6 0.21241285727578685 1108 7 0.253228732408175 1108 8 0.15046201475997378 1108 9 0.23515509041735072 1108 10-11 0.24503582398910595 1108 12-13 0.23575292755793242 1108 14-15 0.2670913527361023 1108 16-17 0.36650158484174256 1108 18-19 0.2027304392250926 1108 20-21 0.17355361446676199 1108 22-23 0.3344946722925144 1108 24-25 0.44368774609469597 1108 26-27 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2314 72-73 -1.4782911127141354 2314 74-75 -1.67299530114812 2314 76-77 -1.3427962977522192 2314 78-79 -1.5684283197951174 2314 80-81 -2.4871720826992814 2314 82-83 -1.4010713221720756 2314 84-85 -1.6350497619327662 2314 86-87 -1.0731756134420714 2314 88-89 -1.0537852784146438 2314 90-91 -1.6904081609112893 2314 92-93 -1.0275127459126203 2314 94-95 -0.9607393969444971 2314 96-97 -1.1852474724109925 2314 98-99 -0.9776134916681514 2314 100-101 -0.8837571554867978 2315 1 -0.7817802467103085 2315 2 -0.6239402723782561 2315 3 -0.7748426878480039 2315 4 -0.6665780229248952 2315 5 -0.5283304908433379 2315 6 -0.6739774458622634 2315 7 -1.250596364939831 2315 8 -0.7658085445325398 2315 9 -3.304371042392056 2315 10-11 -1.2307667097687727 2315 12-13 -0.9060950973307769 2315 14-15 -1.0783688920735202 2315 16-17 -1.474291769484772 2315 18-19 -0.7251123875238576 2315 20-21 -1.021886135795782 2315 22-23 -1.2039246685985248 2315 24-25 -2.3572598767296284 2315 26-27 -1.4820401627551902 2315 28-29 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-1.8967102229401114 2315 94-95 -2.0180710896205802 2315 96-97 -1.9403975299168046 2315 98-99 -2.1181325431114644 2315 100-101 -2.0879352652070438 2316 1 -1.2641300479035031 2316 2 -1.0671032637582982 2316 3 -2.292577853211629 2316 4 -1.4053745905812534 2316 5 -1.2006943656677436 2316 6 -2.7864933817597155 2316 7 -6.956890959595771 2316 8 -7.809109258696502 2316 9 -4.4538403513124365 2316 10-11 -2.202217142961821 2316 12-13 -2.189266508729361 2316 14-15 -2.2183255917652716 2316 16-17 -2.1121930432492277 2316 18-19 -1.6642848570994815 2316 20-21 -2.7468023618232564 2316 22-23 -3.2417973869103704 2316 24-25 -2.0314910268105066 2316 26-27 -2.3220851964916065 2316 28-29 -3.035857411249353 2316 30-31 -3.7261155248297015 2316 32-33 -3.7036898462218275 2316 34-35 -2.8944142005322036 2316 36-37 -3.912679071465618 2316 38-39 -3.4119790115529796 2316 40-41 -4.170250509833984 2316 42-43 -3.5236191700770476 2316 44-45 -3.131822059541232 2316 46-47 -2.931018453979071 2316 48-49 -2.9149013688459036 2316 50-51 -2.596741760787218 2316 52-53 -2.7596605709082205 2316 54-55 -4.4246918037104805 2316 56-57 -3.109370904023624 2316 58-59 -2.6440133657372904 2316 60-61 -3.247114877406446 2316 62-63 -2.9376305887034277 2316 64-65 -4.377975010017302 2316 66-67 -3.3556440423240126 2316 68-69 -3.1451185301014277 2316 70-71 -3.496881467796211 2316 72-73 -3.192155960133956 2316 74-75 -2.9719259602427393 2316 76-77 -3.436295956279764 2316 78-79 -4.4371633887409345 2316 80-81 -3.4891317926368544 2316 82-83 -3.249201769953306 2316 84-85 -3.4164660738049797 2316 86-87 -4.547600687829327 2316 88-89 -4.9938818019012885 2316 90-91 -4.349068320079223 2316 92-93 -4.4825015950855445 2316 94-95 -4.631665011308268 2316 96-97 -4.7775243610370275 2316 98-99 -4.7958793813505025 2316 100-101 -4.913671617407175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74545.0 3 13702.0 4 12455.0 5 11922.0 6 13665.0 7 17045.0 8 21484.0 9 25865.0 10 30463.0 11 36714.0 12 37734.0 13 43230.0 14 49299.0 15 59414.0 16 75107.0 17 100186.0 18 144172.0 19 213038.0 20 275585.0 21 199213.0 22 116618.0 23 104294.0 24 109609.0 25 117448.0 26 129389.0 27 146145.0 28 167084.0 29 195501.0 30 229656.0 31 273304.0 32 329667.0 33 407101.0 34 519957.0 35 715218.0 36 1015592.0 37 1061611.0 38 726177.0 39 101003.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.41806746617002 49.46025370067053 23.179478933555618 16.94219989960384 2 16.23224372210414 51.07279618907402 17.149217449688862 15.545742639132975 3 46.539679313708135 19.650241298973743 18.036824967337463 15.773254419980665 4 16.341662163480567 18.083839130858966 18.9393139014745 46.63518480418596 5 16.238305620859588 48.475714945424755 19.98675018281057 15.29922925090508 6 46.6865943922248 19.18442493317864 17.91197891438715 16.21700176020941 7 17.12836405797277 18.62448249879748 49.03253594104145 15.214617502188299 8 46.23193442556654 19.357714802776247 18.80821550795922 15.602135263697988 9 45.9019092817873 19.02282077317793 19.2953279874033 15.779941957631472 10-11 15.950710512173522 33.960806369622006 34.02222963811068 16.066253480093796 12-13 15.729845240089748 19.360043272026243 33.99291333389433 30.917198153989688 14-15 15.940040216049914 49.171629861974424 18.820017097538926 16.06831282443673 16-17 30.915382600650155 19.151997778330053 18.96615528922077 30.966464331799028 18-19 16.066079676668892 34.04341953900101 18.96655026209505 30.923950522235046 20-21 31.075466090681093 34.089163855536604 18.978227783176678 15.857142270605628 22-23 31.01688792872201 19.094393135912842 33.93493596341131 15.953782971953837 24-25 15.991946380955785 33.84339629386488 19.455668273154565 30.708989052024766 26-27 16.212606157784666 24.97839849699897 42.624128037276165 16.184867307940202 28-29 28.177664209336466 19.00278512581004 36.76008588876537 16.059464776088124 30-31 25.9145899004918 20.198214351799752 37.19305106061622 16.694144687092226 32-33 19.83920882546308 22.657595682692666 38.783294650149465 18.719900841694795 34-35 18.932935424376737 21.770449764341652 39.49594450852856 19.80067030275305 36-37 18.74918165748049 21.936506157946532 39.69235184739659 19.621960337176382 38-39 19.175087569302537 22.087019531036827 39.39455781797308 19.343335081687556 40-41 19.37298979010284 21.7831035906981 39.37056305805126 19.473343561147804 42-43 19.71303535860348 22.059841523444565 39.378403703917044 18.848719414034907 44-45 19.196848756506615 22.189193603581035 39.20451136234588 19.409446277566463 46-47 19.38107241454013 22.000698546757473 39.0702570033102 19.547972035392196 48-49 19.493262647906768 22.061308508209994 39.17011278753482 19.27531605634842 50-51 19.597422707988276 22.109412694211 38.31933467348072 19.97382992432001 52-53 19.957650853990376 22.879667401433487 37.36371071286487 19.798971031711268 54-55 20.492640444325172 23.90592259034229 35.602079922622806 19.999357042709732 56-57 21.05789440508993 24.476665758436905 34.39490107937865 20.070538757094518 58-59 20.9066535258085 25.421819210079384 33.39722911396539 20.274298150146727 60-61 21.05157750077827 25.78591458312294 32.72446241423344 20.43804550186535 62-63 20.950240783442958 25.644388868750173 33.09399318248183 20.311377165325037 64-65 20.72485163280185 25.5986274822266 32.96717532262259 20.709345562348965 66-67 20.72585989644251 25.144247915994427 33.71223023127604 20.417661956287027 68-69 20.524964870744743 24.896080624143398 34.02601759579539 20.552936909316468 70-71 20.393107062431994 24.654469173584083 34.575612420597054 20.376811343386866 72-73 20.146510036620246 24.513143739044462 34.36124380246768 20.97910242186761 74-75 20.597657379221346 24.603398317849905 33.78005148882227 21.01889281410648 76-77 21.04371690374061 25.12056218184234 33.19580000629037 20.639920908126687 78-79 20.832407786655516 25.11303459202418 33.0401121456251 21.0144454756952 80-81 20.79945525162558 25.352318259675222 32.85978811733818 20.98843837136102 82-83 21.835534471381102 25.58404032660977 32.142438296486446 20.437986905522685 84-85 21.261739781320806 26.203316334860748 31.679599792807235 20.85534409101121 86-87 21.17335943370151 25.96683243836491 31.72447874637385 21.135329381559725 88-89 21.123900331410088 25.754578173419585 31.532976288944724 21.5885452062256 90-91 21.15974621567474 25.737308541669794 31.728067940573474 21.37487730208199 92-93 21.18553215456046 26.080626185223643 31.697058684402467 21.036782975813434 94-95 21.325490411557606 25.852936690417117 31.212662358680728 21.608910539344556 96-97 21.084694335321792 25.775358134487607 31.877152141228965 21.262795388961635 98-99 21.309962289863797 26.00802070568443 31.26891150858543 21.413105495866347 100-101 21.882982872817898 25.711932942337068 30.9100182178807 21.495065966964326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10197.0 1 6275.5 2 1737.5 3 1032.0 4 858.5 5 752.0 6 838.0 7 1027.5 8 961.0 9 919.5 10 1275.0 11 9467.5 12 178399.5 13 198269.5 14 50074.5 15 43961.5 16 46855.5 17 50462.0 18 53012.5 19 54483.5 20 56027.5 21 57391.5 22 58963.5 23 61003.5 24 63654.0 25 67576.0 26 72690.0 27 78703.0 28 85123.0 29 91212.5 30 97196.5 31 103926.5 32 111740.0 33 122212.0 34 140769.0 35 151793.5 36 154580.5 37 163400.0 38 172330.5 39 180951.0 40 189770.5 41 197288.5 42 202487.5 43 211625.0 44 224946.0 45 232754.5 46 235551.0 47 242566.5 48 249916.5 49 252849.0 50 254415.5 51 253958.0 52 254249.0 53 258420.5 54 253869.0 55 239947.5 56 236792.0 57 225009.5 58 210939.0 59 181235.0 60 132590.0 61 102016.0 62 78843.5 63 60609.5 64 44854.0 65 30155.5 66 18761.5 67 11350.5 68 7158.0 69 4568.0 70 3108.0 71 2328.0 72 1948.0 73 1699.0 74 1540.0 75 1434.5 76 1357.0 77 1218.5 78 1107.0 79 1049.0 80 984.0 81 907.0 82 846.0 83 752.0 84 628.5 85 570.5 86 518.0 87 411.0 88 328.5 89 269.0 90 212.0 91 163.5 92 124.5 93 86.5 94 58.0 95 42.0 96 24.5 97 19.5 98 19.0 99 13.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12138467414325892 2 0.1099330515670226 3 0.10487009605095782 4 0.092913440505538 5 0.09738298228281214 6 0.08853227949787097 7 0.09763549876740392 8 0.09387300314698668 9 0.08951709378777883 10-11 0.0799972223186695 12-13 0.07187881733904442 14-15 0.07917023083163148 16-17 0.06954303985657063 18-19 0.06213799394591728 20-21 0.05766845216864313 22-23 0.051241907635782846 24-25 0.04840741009624035 26-27 0.04520676365403979 28-29 0.04742890871844728 30-31 0.038357254009488306 32-33 0.05106514609656862 34-35 0.06182234834017759 36-37 0.052037334562246886 38-39 0.05377338539381524 40-41 0.05403221479052179 42-43 0.049228088671163565 44-45 0.04934172108922985 46-47 0.04848316504161787 48-49 0.04906395295617892 50-51 0.050269719170104574 52-53 0.0467723658585087 54-55 0.04710063728847799 56-57 0.04767511229092424 58-59 0.05048435818200757 60-61 0.0461410746470293 62-63 0.054852893365445014 64-65 0.050762126315058505 66-67 0.04557291255669784 68-69 0.04343914826189747 70-71 0.05158280488998172 72-73 0.045654980414190166 74-75 0.04797181916031956 76-77 0.04300355732597669 78-79 0.04775718014841657 80-81 0.04999195103705363 82-83 0.039398884508429315 84-85 0.05019396422472704 86-87 0.05261180956469315 88-89 0.04796550624820477 90-91 0.043862113373588675 92-93 0.04111599660365328 94-95 0.04523201530249897 96-97 0.03256200068810742 98-99 0.03336374052668626 100-101 0.02468979928095931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7920275.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.5488809846713 #Duplication Level Percentage of deduplicated Percentage of total 1 91.48471511344333 40.75541685505346 2 4.637755782920313 4.132136608185761 3 1.2996467065398638 1.7369341935529323 4 0.6141287463257288 1.0943499371732113 5 0.3714046115024126 0.8272829917489529 6 0.2493246437622718 0.666428032890661 7 0.18574126446326805 0.5792195839161539 8 0.13918938974845554 0.4960585246586378 9 0.10852590998634774 0.435123706296146 >10 0.8562999610729699 7.130112995543902 >50 0.03179511051512731 0.9902164988069001 >100 0.01312480341361358 1.3743583072658514 >500 0.003643949181342949 1.1607461180098837 >1k 0.003975217288737573 3.5283411698133795 >5k 5.96282593310636E-4 1.905592890814696 >10k+ 1.3250724295791913E-4 33.18768158626945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1098702 13.872018332696781 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 25723 0.3247740766576918 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9203 0.11619546038489825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8356 0.10550138726243723 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 7921 0.10000915372256645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.5251648459175977E-5 0.0 0.0 0.0 0.0 2 2.5251648459175977E-5 0.0 0.0 0.0 0.0 3 2.5251648459175977E-5 1.2625824229587988E-5 0.0 1.2625824229587988E-5 0.0 4 2.5251648459175977E-5 2.5251648459175977E-5 0.0 1.2625824229587988E-5 0.0 5 2.5251648459175977E-5 2.5251648459175977E-5 0.0 1.2625824229587988E-5 0.0 6 3.787747268876396E-5 2.5251648459175977E-5 0.0 1.2625824229587988E-5 0.0 7 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 8 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 9 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 10-11 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 12-13 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 14-15 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 16-17 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 18-19 3.787747268876396E-5 2.5251648459175977E-5 0.0 2.5251648459175977E-5 0.0 20-21 3.787747268876396E-5 2.5251648459175977E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 22-23 3.787747268876396E-5 2.5251648459175977E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 24-25 3.787747268876396E-5 2.5251648459175977E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 26-27 3.787747268876396E-5 3.787747268876396E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 28-29 4.419038480355796E-5 3.787747268876396E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 30-31 5.050329691835195E-5 3.787747268876396E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 32-33 5.050329691835195E-5 3.787747268876396E-5 0.0 3.787747268876396E-5 1.2625824229587988E-5 34-35 5.050329691835195E-5 3.787747268876396E-5 0.0 4.419038480355796E-5 1.2625824229587988E-5 36-37 5.050329691835195E-5 3.787747268876396E-5 0.0 6.312912114793994E-5 1.2625824229587988E-5 38-39 5.050329691835195E-5 3.787747268876396E-5 0.0 8.838076960711591E-5 1.2625824229587988E-5 40-41 6.944203326273393E-5 3.787747268876396E-5 0.0 1.073195059514979E-4 1.2625824229587988E-5 42-43 9.46936817219099E-5 3.787747268876396E-5 0.0 1.1994533018108589E-4 1.2625824229587988E-5 44-45 1.010065938367039E-4 3.787747268876396E-5 0.0 1.262582422958799E-4 1.2625824229587988E-5 46-47 1.073195059514979E-4 3.787747268876396E-5 0.0 1.5150989075505585E-4 1.2625824229587988E-5 48-49 1.1994533018108589E-4 3.787747268876396E-5 0.0 1.5150989075505585E-4 1.2625824229587988E-5 50-51 1.6413571498464385E-4 3.787747268876396E-5 0.0 1.5150989075505585E-4 1.2625824229587988E-5 52-53 1.957002755586138E-4 3.787747268876396E-5 0.0 1.5150989075505585E-4 1.2625824229587988E-5 54-55 2.5882939670655375E-4 3.787747268876396E-5 0.0 1.5150989075505585E-4 1.2625824229587988E-5 56-57 3.6614890265805166E-4 6.312912114793994E-5 0.0 1.5782280286984985E-4 1.2625824229587988E-5 58-59 4.860942328391375E-4 7.575494537752792E-5 0.0 1.6413571498464385E-4 1.2625824229587988E-5 60-61 5.934137387906354E-4 7.575494537752792E-5 0.0 1.7044862709943783E-4 1.2625824229587988E-5 62-63 7.322978053161033E-4 7.575494537752792E-5 0.0 1.7676153921423183E-4 1.2625824229587988E-5 64-65 8.459302233823952E-4 7.575494537752792E-5 0.0 1.7676153921423183E-4 1.2625824229587988E-5 66-67 9.84814289907863E-4 7.575494537752792E-5 0.0 1.7676153921423183E-4 1.2625824229587988E-5 68-69 0.001155262917007301 7.575494537752792E-5 0.0 1.7676153921423183E-4 1.2625824229587988E-5 70-71 0.0013193986319919447 8.838076960711591E-5 0.0 1.7676153921423183E-4 1.2625824229587988E-5 72-73 0.0015656022044689105 8.838076960711591E-5 0.0 1.8307445132902583E-4 1.2625824229587988E-5 74-75 0.0017991799527162882 8.838076960711591E-5 0.0 1.893873634438198E-4 1.2625824229587988E-5 76-77 0.00213376429480037 8.838076960711591E-5 0.0 1.893873634438198E-4 1.2625824229587988E-5 78-79 0.0024999131974584215 8.838076960711591E-5 0.0 1.893873634438198E-4 1.2625824229587988E-5 80-81 0.0028660621001164732 8.838076960711591E-5 0.0 2.020131876734078E-4 1.2625824229587988E-5 82-83 0.0034279112783331387 9.46936817219099E-5 0.0 2.209519240177898E-4 1.2625824229587988E-5 84-85 0.004147583259419654 1.010065938367039E-4 0.0 2.3989066036217177E-4 1.2625824229587988E-5 86-87 0.005189213758360662 1.010065938367039E-4 0.0 2.3989066036217177E-4 1.893873634438198E-5 88-89 0.006533864038811784 1.010065938367039E-4 0.0 2.4620357247696575E-4 2.5251648459175977E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 238395 0.0 91.88546 5 GGTATCA 239660 0.0 91.75701 8 GTATCAA 240260 0.0 91.6663 9 TGGTATC 239540 0.0 91.524284 7 GTGGTAT 240195 0.0 91.39233 6 AAGCAGT 241545 0.0 91.308075 1 CAGTGGT 241820 0.0 90.6177 4 GCAGTGG 242740 0.0 90.55755 3 AGCAGTG 243215 0.0 90.54286 2 TCAACGC 239605 0.0 46.510708 12-13 AACGCAG 240710 0.0 46.356228 14-15 CAACGCA 239620 0.0 46.32638 12-13 ATCAACG 239615 0.0 46.300938 10-11 CGCAGAG 241335 0.0 46.199093 16-17 ACGCAGA 240905 0.0 46.113605 14-15 TATCAAC 241125 0.0 46.111477 10-11 CAGAGTA 239495 0.0 45.95644 18-19 AGAGTAC 235205 0.0 45.860603 18-19 GAGTACT 138650 0.0 45.723995 20-21 GCAGAGT 242770 0.0 45.503376 16-17 >>END_MODULE