FastQCFastQC Report
Sat 18 Jun 2016
SRR578567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920275
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1222771.5438479093213302No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214010.27020526433741254No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87770.11081685926309377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3050.031.13223
CAACGCA55650.020.09079712-13
ATCAACG59200.019.28700410-11
TCAACGC55250.018.7754610-11
CGTACGC2750.018.12731620-21
CGCGTAG2650.017.91660554-55
ACTCGCG2800.017.80597350-51
AACGCAG58500.017.52949112-13
CTCGCGT2850.017.49303852-53
CGTAGCG3150.017.33305556-57
ACGTACG3050.016.34430120-21
GGTATCA71900.016.1773957
ACGCAGA72400.015.57386714-15
GTATCAA75100.015.4248638
AGTGGTA76450.015.0285644
TATCAAC80150.014.5714589
TGGTATC83650.014.0185296
CGCTAGT3700.013.47368590-91
CGCAGAG77150.013.4150414-15
GTGGTAT90100.013.067845
Produced by FastQC (version 0.11.5)