##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7920275 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.390606765547915 31.0 31.0 34.0 30.0 34.0 2 31.762342595427558 31.0 31.0 34.0 30.0 34.0 3 31.836933187294633 31.0 31.0 34.0 30.0 34.0 4 35.54465293187421 37.0 35.0 37.0 33.0 37.0 5 35.369169757363224 37.0 35.0 37.0 33.0 37.0 6 35.36007865888495 37.0 35.0 37.0 32.0 37.0 7 35.35404351490321 37.0 35.0 37.0 32.0 37.0 8 35.310622169053474 37.0 35.0 37.0 32.0 37.0 9 36.84999498123487 39.0 37.0 39.0 32.0 39.0 10-11 36.842653821994816 39.0 37.0 39.0 32.0 39.0 12-13 36.83402501807071 39.0 37.0 39.0 32.0 39.0 14-15 38.00200864237669 40.0 37.0 41.0 33.0 41.0 16-17 37.928618766393846 40.0 37.0 41.0 33.0 41.0 18-19 37.846297256092754 40.0 37.0 41.0 32.0 41.0 20-21 37.796038142615 40.0 37.0 41.0 32.0 41.0 22-23 37.58539570911364 39.0 36.0 41.0 32.0 41.0 24-25 37.586559178816394 39.0 36.0 41.0 32.0 41.0 26-27 37.41113591384138 39.0 36.0 41.0 32.0 41.0 28-29 37.25664550788956 39.0 36.0 41.0 31.0 41.0 30-31 37.11318350688581 39.0 36.0 41.0 31.0 41.0 32-33 36.97122221134998 39.0 36.0 40.0 30.5 41.0 34-35 36.82002474661549 39.0 35.0 40.0 30.0 41.0 36-37 36.6647940885891 38.5 35.0 40.0 30.0 41.0 38-39 36.49228776778584 38.0 35.0 40.0 30.0 41.0 40-41 36.325055001246795 38.0 35.0 40.0 30.0 41.0 42-43 36.403589269311986 38.0 35.0 40.0 30.0 41.0 44-45 36.33285428851902 38.0 35.0 40.0 30.0 41.0 46-47 36.1770483348116 38.0 35.0 40.0 29.5 41.0 48-49 36.02101246484497 38.0 35.0 40.0 29.0 41.0 50-51 35.8261001038474 38.0 34.0 40.0 28.0 41.0 52-53 35.87227880345064 38.0 34.0 40.0 28.0 41.0 54-55 35.85455669152902 38.0 34.0 40.0 28.0 41.0 56-57 35.68516061626649 38.0 34.0 40.0 28.0 41.0 58-59 35.44492419770778 38.0 34.0 40.0 28.0 41.0 60-61 35.174358276701255 37.5 34.0 40.0 27.5 41.0 62-63 34.88030093652051 37.0 33.5 40.0 27.0 41.0 64-65 34.54223660163315 36.5 33.0 39.5 26.0 41.0 66-67 34.18715069868155 36.0 33.0 39.0 26.0 41.0 68-69 33.79770462515506 36.0 32.0 39.0 26.0 40.5 70-71 33.39049370129194 35.0 32.0 38.5 26.0 40.0 72-73 32.991953309702 35.0 32.0 37.5 25.5 40.0 74-75 32.57478774411242 35.0 31.0 37.0 25.0 39.0 76-77 31.209700483884713 33.5 29.5 35.5 22.5 38.5 78-79 31.861824305342935 34.0 31.0 36.0 24.0 39.0 80-81 31.73985720192796 34.0 31.0 36.0 24.0 37.5 82-83 31.37647209219377 34.0 31.0 35.0 23.0 37.0 84-85 31.100829137877156 34.0 31.0 35.0 22.5 37.0 86-87 30.789152965016996 34.0 31.0 35.0 20.5 36.0 88-89 30.48073677997292 34.0 30.0 35.0 20.0 36.0 90-91 30.14158914936666 34.0 30.0 35.0 18.5 36.0 92-93 29.76513511210154 34.0 30.0 35.0 17.0 35.0 94-95 29.376488947669117 34.0 29.0 35.0 8.5 35.0 96-97 28.89698703643497 34.0 29.0 35.0 2.0 35.0 98-99 28.07059773808359 34.0 29.0 35.0 2.0 35.0 100-101 27.001944124414873 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36573707727535165 1101 2 0.28262782861962776 1101 3 0.396878894411131 1101 4 0.3190383570346853 1101 5 0.29731262610037135 1101 6 0.2334520377331728 1101 7 0.30552711945269806 1101 8 0.2662773160169749 1101 9 0.3467952087018631 1101 10-11 0.36013842476722857 1101 12-13 0.3048313402965803 1101 14-15 0.2694175471961202 1101 16-17 0.3115138809318623 1101 18-19 0.42064889626007584 1101 20-21 0.34458982909124813 1101 22-23 0.4960945459883561 1101 24-25 0.4297031714265742 1101 26-27 0.38995890018865254 1101 28-29 0.4155228639516224 1101 30-31 0.4330976533345563 1101 32-33 0.49394622515956854 1101 34-35 0.4405423652926501 1101 36-37 0.42076633955912257 1101 38-39 0.3528456953955086 1101 40-41 0.34315610351258385 1101 42-43 0.32675304620687484 1101 44-45 0.3379514463405968 1101 46-47 0.36388271414325146 1101 48-49 0.4374159102182986 1101 50-51 0.4686871334154361 1101 52-53 0.4602022882398913 1101 54-55 0.5613536321206567 1101 56-57 0.5183843631478666 1101 58-59 0.5447658209159485 1101 60-61 0.41851703445284016 1101 62-63 0.4379370084364922 1101 64-65 0.4087318429271889 1101 66-67 0.42519656856087806 1101 68-69 0.29666178036315216 1101 70-71 0.3845295821614769 1101 72-73 0.5849466034605939 1101 74-75 0.5649421606827616 1101 76-77 0.4858916378386624 1101 78-79 0.4653386069199428 1101 80-81 0.44970030978374 1101 82-83 0.5910383730770654 1101 84-85 0.601186273961293 1101 86-87 0.5555411655832927 1101 88-89 0.6352309149462378 1101 90-91 0.5520795049122142 1101 92-93 0.6674091775462934 1101 94-95 0.5126719542082014 1101 96-97 0.45163322472636835 1101 98-99 0.7614978141933086 1101 100-101 0.9669349326232641 1102 1 0.30047271618494165 1102 2 0.33036773459333446 1102 3 0.3191304322286541 1102 4 0.2872458846583612 1102 5 0.34054701285940325 1102 6 0.22562544356427594 1102 7 0.31156202510439357 1102 8 0.1554110780807676 1102 9 0.281903331580061 1102 10-11 0.28672595478295904 1102 12-13 0.35526449790561543 1102 14-15 0.37593398523720367 1102 16-17 0.25914207409977763 1102 18-19 0.3342123308878797 1102 20-21 0.27663669135468894 1102 22-23 0.43194540666935666 1102 24-25 0.3919813457340311 1102 26-27 0.4730147878723088 1102 28-29 0.3993974759321546 1102 30-31 0.4388713241321227 1102 32-33 0.3762634231391999 1102 34-35 0.3615933915776921 1102 36-37 0.4432216218680196 1102 38-39 0.431382955384656 1102 40-41 0.3770766025327603 1102 42-43 0.28407032698852674 1102 44-45 0.4293222834690198 1102 46-47 0.2964817297192326 1102 48-49 0.26969045227712485 1102 50-51 0.3784272270737148 1102 52-53 0.3465795968062224 1102 54-55 0.33801631325007975 1102 56-57 0.38832204082720523 1102 58-59 0.4574537106604595 1102 60-61 0.4527762783783942 1102 62-63 0.2511796275564038 1102 64-65 0.25991631294824913 1102 66-67 0.29357920957732375 1102 68-69 0.29687538013499193 1102 70-71 0.3801202678542097 1102 72-73 0.41786873092050314 1102 74-75 0.24492413560294324 1102 76-77 0.11879718281396023 1102 78-79 0.273077497188865 1102 80-81 0.32600077439508013 1102 82-83 0.34908292792420426 1102 84-85 0.30735009315848316 1102 86-87 0.37875944826938834 1102 88-89 0.2579285679689711 1102 90-91 0.34104998875201886 1102 92-93 0.2605347256496664 1102 94-95 0.31670064418823785 1102 96-97 0.5142578353268696 1102 98-99 0.8619876709458154 1102 100-101 0.9621416451070495 1103 1 0.2177442281050972 1103 2 0.2466608375581636 1103 3 0.25237525165743335 1103 4 0.2401539602706393 1103 5 0.24073108431801415 1103 6 0.09826239130812553 1103 7 0.23512989418126296 1103 8 0.0671903935857685 1103 9 0.21018163946740032 1103 10-11 0.1972421320923985 1103 12-13 0.29016354947126644 1103 14-15 0.34772936100888074 1103 16-17 0.2926229310854467 1103 18-19 0.2717879334071185 1103 20-21 0.4193511489322148 1103 22-23 0.3802420862763398 1103 24-25 0.3329854170380244 1103 26-27 0.18778861967171423 1103 28-29 0.25698842244876374 1103 30-31 0.2810794478681231 1103 32-33 0.25797748513355145 1103 34-35 0.22320806769194235 1103 36-37 0.22572974598122642 1103 38-39 0.31263546542881926 1103 40-41 0.3090562686310676 1103 42-43 0.2822047495101643 1103 44-45 0.25601494408520864 1103 46-47 0.3836192182435454 1103 48-49 0.2728743510938898 1103 50-51 0.17404398306535995 1103 52-53 0.14120859964045707 1103 54-55 0.2914694290710429 1103 56-57 0.228440577229982 1103 58-59 0.12741984879237833 1103 60-61 0.25205877991208325 1103 62-63 0.30846400392572093 1103 64-65 0.18502051801274177 1103 66-67 0.15495632208232735 1103 68-69 0.30168676214476875 1103 70-71 0.2062868635178532 1103 72-73 0.11736383850999488 1103 74-75 0.18617030277690105 1103 76-77 0.15389626484614283 1103 78-79 0.31043142195956364 1103 80-81 0.25859506381253894 1103 82-83 0.49237152684019847 1103 84-85 0.3217277150121518 1103 86-87 0.3129276369366174 1103 88-89 0.39470054329126825 1103 90-91 0.34320269068435394 1103 92-93 0.2703623521567877 1103 94-95 0.4492313055416055 1103 96-97 0.33252286469426906 1103 98-99 0.8645839248688212 1103 100-101 0.63349739445691 1104 1 0.24151804059064474 1104 2 0.24730134018269112 1104 3 0.17399241836644563 1104 4 0.18698778207068045 1104 5 0.21290366723170706 1104 6 0.12688494644966397 1104 7 0.16623223854587366 1104 8 0.08208428848066518 1104 9 0.26653677923746244 1104 10-11 0.15295570102330913 1104 12-13 0.18194291064993706 1104 14-15 0.3105389220070478 1104 16-17 0.37233182782741636 1104 18-19 0.16594447696461145 1104 20-21 0.2408186775230874 1104 22-23 0.32494778164159044 1104 24-25 0.2544807050041129 1104 26-27 0.3027006784766968 1104 28-29 0.297738438095557 1104 30-31 0.2127613057683675 1104 32-33 0.2974276313155997 1104 34-35 0.2223793561624774 1104 36-37 0.19575680846004673 1104 38-39 0.20571239486519488 1104 40-41 0.23922391099532092 1104 42-43 0.1814859603905319 1104 44-45 0.1725918778825246 1104 46-47 0.205369106357999 1104 48-49 0.23822659671257185 1104 50-51 0.18669380336240238 1104 52-53 0.14738718427255293 1104 54-55 0.24206754971218913 1104 56-57 0.19376653653004183 1104 58-59 0.16995366922021304 1104 60-61 0.19963689303538956 1104 62-63 0.09062284664472031 1104 64-65 0.18301208275369163 1104 66-67 0.10271845364096777 1104 68-69 0.11577928441796814 1104 70-71 0.15727797208001704 1104 72-73 0.19926334940121393 1104 74-75 0.15111183975989917 1104 76-77 0.11879992424545449 1104 78-79 0.13845006792991654 1104 80-81 0.22547146135190843 1104 82-83 0.3288885872078744 1104 84-85 0.28570241122370277 1104 86-87 0.3300160949656856 1104 88-89 0.3619250268045313 1104 90-91 0.29852687975959213 1104 92-93 0.32566546388018836 1104 94-95 0.36646789027876636 1104 96-97 0.3592375984620908 1104 98-99 0.6541542025810436 1104 100-101 0.7645081507815554 1105 1 0.22082462622975996 1105 2 0.1728211782095208 1105 3 0.07849556477246722 1105 4 0.1305997107626098 1105 5 0.20803650107265526 1105 6 0.06104562378209266 1105 7 0.1867960177014254 1105 8 0.04523955646624955 1105 9 0.22082854942453878 1105 10-11 0.06070429338468131 1105 12-13 0.08313250781301207 1105 14-15 0.10533509688946197 1105 16-17 0.08848829163162719 1105 18-19 0.1453812098638565 1105 20-21 0.11986361169673643 1105 22-23 0.2515322242562945 1105 24-25 0.19953411061226944 1105 26-27 0.17243223204410896 1105 28-29 0.18778320533934334 1105 30-31 0.13078538702258413 1105 32-33 0.17566548179150487 1105 34-35 0.15392134260736867 1105 36-37 0.15563791275058492 1105 38-39 0.22531871689850647 1105 40-41 0.27944423622520986 1105 42-43 0.2643674480394296 1105 44-45 0.22137141234961177 1105 46-47 0.16847491838473871 1105 48-49 0.1724072868531934 1105 50-51 0.2373335902247149 1105 52-53 0.27595842509055757 1105 54-55 0.17825626272732364 1105 56-57 0.2275538747121928 1105 58-59 0.15783452704172163 1105 60-61 -0.015754560906650283 1105 62-63 0.03720768012792064 1105 64-65 0.09344968819430477 1105 66-67 0.1427969823641746 1105 68-69 0.18762744207896276 1105 70-71 0.2677109092877643 1105 72-73 0.33557220753951356 1105 74-75 0.39695075449049 1105 76-77 0.18001078363043277 1105 78-79 0.23231039440239698 1105 80-81 0.38311000460381095 1105 82-83 0.5085240229699188 1105 84-85 0.3779964047526967 1105 86-87 0.3847984477823623 1105 88-89 0.27168484238287505 1105 90-91 0.5213666400633272 1105 92-93 0.6290720534791951 1105 94-95 0.5854638259291818 1105 96-97 0.5872066969793828 1105 98-99 1.0753612591057475 1105 100-101 1.1192806209407102 1106 1 0.1097857532812796 1106 2 0.1413046062948773 1106 3 0.12893779327308152 1106 4 0.11310781802323078 1106 5 0.09951387319932792 1106 6 0.0022427991346205545 1106 7 0.07332527210581219 1106 8 0.03481246873533195 1106 9 0.07680206494993769 1106 10-11 -0.026103564625906017 1106 12-13 0.08078747373057382 1106 14-15 -0.0064416965287747985 1106 16-17 0.021362212074841125 1106 18-19 0.047026616250008146 1106 20-21 0.08662508713261019 1106 22-23 0.15110190741403784 1106 24-25 0.06866948484174173 1106 26-27 0.0836075761945736 1106 28-29 0.11943149950605658 1106 30-31 0.09636084713821447 1106 32-33 0.12318886513197214 1106 34-35 0.2209972264620177 1106 36-37 0.1104897594810268 1106 38-39 0.055456766427958826 1106 40-41 0.10347951411385736 1106 42-43 0.18360459296376064 1106 44-45 0.008193495385697247 1106 46-47 -0.052696707791888286 1106 48-49 0.02700987045653136 1106 50-51 0.0791806602448375 1106 52-53 0.07899323978199391 1106 54-55 0.108746061913088 1106 56-57 0.0999521659981184 1106 58-59 0.0531684567872901 1106 60-61 0.1852780257939841 1106 62-63 0.17814280772626034 1106 64-65 -0.013301424229744896 1106 66-67 0.024787497733484543 1106 68-69 0.10857023696168255 1106 70-71 0.15644155076962818 1106 72-73 0.27036142138228314 1106 74-75 0.29776769172508466 1106 76-77 0.09193452387544099 1106 78-79 0.2924885812882394 1106 80-81 0.3753030015788035 1106 82-83 0.5016303853057202 1106 84-85 0.5466771630552394 1106 86-87 0.5812096907815878 1106 88-89 0.5569409504578005 1106 90-91 0.5252020281904635 1106 92-93 0.5459123754510138 1106 94-95 0.47106712239878945 1106 96-97 0.4641234744257794 1106 98-99 1.0354850076614213 1106 100-101 1.0621936599858088 1107 1 0.1588878087524641 1107 2 0.03137195320669761 1107 3 0.003486350758461043 1107 4 0.0028356198945260758 1107 5 -0.022799879798640177 1107 6 -0.0175946824178439 1107 7 0.009947128891113266 1107 8 0.07011308760734636 1107 9 0.10042314640832473 1107 10-11 0.0019681557724240406 1107 12-13 0.01588825393508131 1107 14-15 -0.03969779022719422 1107 16-17 -0.05745044186190995 1107 18-19 -0.09719527551906992 1107 20-21 -0.03475335165438054 1107 22-23 0.09637335674814551 1107 24-25 0.10817311007688346 1107 26-27 0.04853077371389958 1107 28-29 -0.015897334255079443 1107 30-31 0.026514736864527322 1107 32-33 -0.008918050442140668 1107 34-35 0.09284231810057264 1107 36-37 0.18794708519385495 1107 38-39 0.19000388321996553 1107 40-41 0.03730966651400536 1107 42-43 0.05830217315354247 1107 44-45 0.08921298480213125 1107 46-47 0.027178325280580395 1107 48-49 0.04903903433345391 1107 50-51 0.07996131935219353 1107 52-53 0.012262410939278823 1107 54-55 0.012837539624200645 1107 56-57 0.09677652926396263 1107 58-59 0.020071814309062574 1107 60-61 0.11733487305595958 1107 62-63 0.18533672528406697 1107 64-65 0.1972470859663531 1107 66-67 0.1896374368949978 1107 68-69 0.22000033628576432 1107 70-71 0.16452826078347016 1107 72-73 0.18373537174752386 1107 74-75 0.19072856936610094 1107 76-77 0.12893278932521213 1107 78-79 0.24728084382414295 1107 80-81 0.18521055046331014 1107 82-83 0.27994323386045394 1107 84-85 0.31578608519300744 1107 86-87 0.34525961768333957 1107 88-89 0.36011671013264746 1107 90-91 0.4984789386073558 1107 92-93 0.5135000694764464 1107 94-95 0.48053802310862537 1107 96-97 0.5436810774871077 1107 98-99 1.0020367476524896 1107 100-101 1.0185303078002335 1108 1 0.018488084504181046 1108 2 -0.034770286624400626 1108 3 -0.0656302944544791 1108 4 -0.016942370927360173 1108 5 -0.04387483183728591 1108 6 -0.041733363117600675 1108 7 -0.08035088840196636 1108 8 0.03215521503106089 1108 9 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2316 90-91 -1.1632399506812305 2316 92-93 -1.1537861589858842 2316 94-95 -1.2960966850771882 2316 96-97 -1.3832205278764604 2316 98-99 -1.143806548502969 2316 100-101 -1.6528469184797352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93.0 3 74.0 4 221.0 5 618.0 6 1216.0 7 2118.0 8 3652.0 9 5581.0 10 7709.0 11 9608.0 12 11807.0 13 14546.0 14 17001.0 15 19584.0 16 22706.0 17 26280.0 18 30299.0 19 34926.0 20 37474.0 21 39802.0 22 44429.0 23 53166.0 24 65074.0 25 82171.0 26 104170.0 27 132060.0 28 166290.0 29 204100.0 30 249469.0 31 302313.0 32 368264.0 33 456344.0 34 587696.0 35 818552.0 36 1048116.0 37 1447956.0 38 1257761.0 39 246744.0 40 285.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.793283830388276 31.119286989650263 27.690054926037078 22.397374253924383 2 23.184687400374358 28.99262967510598 27.717017401542243 20.10566552297742 3 21.44279081218771 28.158555100675166 28.230421292189984 22.168232794947144 4 21.96018732879203 28.59515274377187 27.923350848060245 21.521309079375854 5 22.601797194602735 28.406904190823678 27.78483915814093 21.206459456432658 6 22.302786295308767 28.300804543685782 27.542155781131594 21.85425337987386 7 22.456967631992843 28.610592153615176 27.549970272436795 21.382469941955183 8 21.703933475028766 28.43859947269501 27.843039268590957 22.01442778368526 9 21.513291803536173 28.271607853129012 28.254323060476445 21.96077728285837 10-11 21.607131234592117 28.75093486567447 27.707180658502743 21.934753241230666 12-13 21.58235667322157 28.79021877396934 27.5789540135917 22.048470539217387 14-15 21.777450909217166 28.776721767867908 27.412987554093764 22.03283976882116 16-17 21.943517603496748 28.631639338694516 27.33662715919009 22.088215898618646 18-19 21.913479376690717 28.66648481267724 27.313128770111284 22.10690704052076 20-21 22.00142179415625 28.578296118398423 27.37209423739038 22.048187850054944 22-23 22.10064753137344 28.580885362730445 27.29127700014924 22.027190105746875 24-25 21.951441080013005 28.537544466574712 27.314083159991288 22.196931293421 26-27 22.114768559093662 28.414907765355977 27.442957533662245 22.02736614188812 28-29 22.121327443159643 28.518829317246514 27.333365993855697 22.02647724573815 30-31 22.105006179931266 28.540070310685962 27.28748681658855 22.06743669279422 32-33 22.104185376578844 28.56475040551032 27.20987815183503 22.1211860660758 34-35 22.11074998846306 28.60717618734406 27.190452120042043 22.091621704150842 36-37 22.123034016454422 28.56510480963299 27.18914280443883 22.122718369473755 38-39 22.11834067007273 28.558726847920852 27.19718668187675 22.125745800129668 40-41 22.12773060351685 28.649701946100297 27.15011431754672 22.07245313283613 42-43 22.13918542033688 28.6313397051558 27.112889942063205 22.116584932444113 44-45 22.128823441773083 28.651072823734697 27.123507932860452 22.09659580163177 46-47 22.14654153499711 28.69708329500545 27.063586319241068 22.092788850756374 48-49 22.160237446425622 28.682930457193716 27.050341071647964 22.106491024732698 50-51 22.17985090405649 28.670465943290157 27.034746606406546 22.114936546246806 52-53 22.136274456693652 28.73344629998847 26.986927007705752 22.143352235612127 54-55 22.154790057490008 28.761222963527793 26.924449333450003 22.159537645532197 56-57 22.160106901145166 28.774744673897406 26.92955474880131 22.13559367615612 58-59 22.11932950934134 28.88490398564677 26.904820224818405 22.090946280193478 60-61 22.165557519502638 28.828331644313593 26.870110373068506 22.136000463115263 62-63 22.11269166969897 28.903707274477902 26.88367410892513 22.099926946897995 64-65 22.112199178569707 28.966329741656455 26.84720019085354 22.0742708889203 66-67 22.101460551806102 28.96667334369377 26.836680653491772 22.09518545100835 68-69 22.06281339716785 29.013996355079037 26.85658268836784 22.066607559385268 70-71 22.11197709734129 29.08142210066415 26.75859362635341 22.048007175641153 72-73 22.08945927369605 29.05417200110836 26.772888108766356 22.083480616429235 74-75 22.13831770603302 29.049461914310832 26.759008771843433 22.053211607812717 76-77 22.070268173328625 29.193769189564726 26.729758846336072 22.006203790770577 78-79 22.087953462456422 29.136191101600062 26.738879588411475 22.036975847532045 80-81 22.0618899421521 29.194521942670264 26.685252352418544 22.058335762759093 82-83 22.106996389817855 29.273990135729367 26.634042074474067 21.98497139997871 84-85 22.017776407454644 29.28943232133539 26.635704350089952 22.057086921120018 86-87 21.996567986059826 29.331415099068437 26.629692109890605 22.04232480498113 88-89 22.008895797756047 29.39332836000955 26.63159330761573 21.96618253461867 90-91 21.990084205655354 29.352948176448994 26.61362081792835 22.0433467999673 92-93 22.005691540187996 29.423954720198136 26.584920587623305 21.98543315199056 94-95 21.998330725130806 29.485162979672687 26.55289689137485 21.963609403821657 96-97 21.970319802040265 29.51452025211748 26.509436981771856 22.005722964070397 98-99 21.995553118916114 29.542436601923793 26.44871761835448 22.013292660805615 100-101 22.01176893405689 29.538726545352024 26.416726171248072 22.032778349343015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 136547.0 1 77794.5 2 14097.5 3 8279.0 4 7003.0 5 6211.5 6 5689.0 7 5508.5 8 5265.0 9 4876.0 10 4684.5 11 5112.0 12 8259.5 13 8165.0 14 5288.5 15 5507.0 16 5941.0 17 6523.0 18 7733.5 19 9480.0 20 12145.0 21 15691.0 22 19644.5 23 24140.0 24 29843.0 25 37036.0 26 47052.0 27 58593.5 28 67281.5 29 75284.5 30 87270.0 31 102098.5 32 118025.0 33 136325.5 34 153708.5 35 168741.0 36 185490.5 37 201948.0 38 213060.5 39 218775.0 40 226825.5 41 238252.0 42 247290.5 43 258486.0 44 269636.0 45 278443.0 46 286364.5 47 291898.0 48 300721.0 49 307344.0 50 306098.0 51 300317.0 52 292457.5 53 284167.0 54 271451.5 55 253173.5 56 230418.5 57 207539.5 58 188564.0 59 163565.0 60 132957.5 61 104147.0 62 77680.0 63 56699.5 64 40450.5 65 26283.0 66 15777.0 67 9864.5 68 5664.0 69 2805.5 70 1413.0 71 668.5 72 344.5 73 213.5 74 147.5 75 95.5 76 56.0 77 35.0 78 29.0 79 22.0 80 14.5 81 12.5 82 9.5 83 7.5 84 4.0 85 2.5 86 3.0 87 2.5 88 2.5 89 1.5 90 1.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16075199409111426 2 0.0 3 0.0 4 0.001047943411055803 5 5.302846176426955E-4 6 1.136324180662919E-4 7 2.020131876734078E-4 8 1.2625824229587988E-5 9 2.272648361325838E-4 10-11 2.4620357247696575E-4 12-13 0.0 14-15 0.0 16-17 2.5251648459175977E-5 18-19 1.893873634438198E-5 20-21 6.312912114793994E-6 22-23 5.176587934131075E-4 24-25 0.0 26-27 1.7044862709943783E-4 28-29 0.005277594527967779 30-31 0.003585734081202988 32-33 1.010065938367039E-4 34-35 8.396173112676011E-4 36-37 4.3559093592078556E-4 38-39 0.001136324180662919 40-41 0.002916565397034825 42-43 0.001161575829122095 44-45 6.944203326273394E-4 46-47 0.008465615145938745 48-49 0.008560308827660654 50-51 0.007695439867933879 52-53 0.014191426434056898 54-55 0.005858382442528827 56-57 7.638623658900733E-4 58-59 0.0013257115441067386 60-61 6.312912114793994E-6 62-63 1.136324180662919E-4 64-65 8.269914870380131E-4 66-67 0.001054256323170597 68-69 0.0026893005609022413 70-71 0.0018496832496346402 72-73 0.005511172276215157 74-75 0.002032757700963666 76-77 0.0014835343469765886 78-79 0.0016666087983056143 80-81 2.7776813305093573E-4 82-83 0.002222145064407486 84-85 2.5251648459175977E-5 86-87 0.0018181186890606704 88-89 2.3357774824737777E-4 90-91 0.001041630498941009 92-93 0.001249956598729211 94-95 8.774947839563651E-4 96-97 9.911272020226571E-4 98-99 0.5352591923891531 100-101 2.083260997882018E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7920275.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.44763403599279 #Duplication Level Percentage of deduplicated Percentage of total 1 90.93198888523673 59.51283530725938 2 5.024835993467788 6.577272543827281 3 1.2985434812391985 2.5495979561990145 4 0.6129164440819526 1.6045572450767067 5 0.36734762908520757 1.2021016596179144 6 0.22197419293624102 0.8716611446835575 7 0.16196669525747942 0.7420235898061478 8 0.13167478544085404 0.689424253544071 9 0.09752215531455423 0.5744334898285375 >10 1.0741407530263871 14.981608259569382 >50 0.07055236810067454 3.0160317499522393 >100 0.006137228357429876 0.5479433859655025 >500 0.0 0.0 >1k 1.3312948515682504E-4 0.2677943569389194 >5k 1.3312948515682504E-4 0.6033759303081233 >10k+ 1.3312948515682504E-4 6.259339127423144 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122277 1.5438479093213302 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21401 0.27020526433741254 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8777 0.11081685926309377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2625824229587988E-5 0.0 0.0 0.0 0.0 2 1.2625824229587988E-5 0.0 0.0 0.0 0.0 3 1.2625824229587988E-5 0.0 0.0 0.0 0.0 4 1.2625824229587988E-5 0.0 0.0 0.0 0.0 5 1.2625824229587988E-5 0.0 0.0 0.0 0.0 6 2.5251648459175977E-5 0.0 0.0 1.2625824229587988E-5 0.0 7 2.5251648459175977E-5 0.0 0.0 1.2625824229587988E-5 0.0 8 2.5251648459175977E-5 0.0 0.0 1.2625824229587988E-5 0.0 9 2.5251648459175977E-5 0.0 0.0 1.2625824229587988E-5 0.0 10-11 2.5251648459175977E-5 6.312912114793994E-6 0.0 1.2625824229587988E-5 0.0 12-13 3.156456057396997E-5 2.5251648459175977E-5 0.0 1.2625824229587988E-5 0.0 14-15 3.787747268876396E-5 3.156456057396997E-5 0.0 2.5251648459175977E-5 0.0 16-17 3.787747268876396E-5 3.787747268876396E-5 0.0 2.5251648459175977E-5 0.0 18-19 3.787747268876396E-5 3.787747268876396E-5 0.0 3.787747268876396E-5 0.0 20-21 3.787747268876396E-5 3.787747268876396E-5 0.0 5.050329691835195E-5 0.0 22-23 3.787747268876396E-5 3.787747268876396E-5 0.0 5.050329691835195E-5 0.0 24-25 3.787747268876396E-5 3.787747268876396E-5 0.0 5.050329691835195E-5 0.0 26-27 3.787747268876396E-5 6.312912114793994E-5 0.0 5.050329691835195E-5 0.0 28-29 5.050329691835195E-5 6.312912114793994E-5 0.0 6.312912114793994E-5 0.0 30-31 5.050329691835195E-5 6.312912114793994E-5 0.0 7.575494537752792E-5 0.0 32-33 5.050329691835195E-5 6.312912114793994E-5 0.0 7.575494537752792E-5 0.0 34-35 5.050329691835195E-5 7.575494537752792E-5 0.0 7.575494537752792E-5 0.0 36-37 5.050329691835195E-5 8.838076960711591E-5 0.0 7.575494537752792E-5 0.0 38-39 5.050329691835195E-5 8.838076960711591E-5 0.0 8.206785749232193E-5 0.0 40-41 9.46936817219099E-5 8.838076960711591E-5 0.0 8.838076960711591E-5 0.0 42-43 1.1994533018108589E-4 8.838076960711591E-5 0.0 8.838076960711591E-5 0.0 44-45 1.262582422958799E-4 8.838076960711591E-5 0.0 8.838076960711591E-5 0.0 46-47 1.3257115441067386E-4 1.010065938367039E-4 0.0 8.838076960711591E-5 0.0 48-49 1.5150989075505585E-4 1.1363241806629188E-4 0.0 8.838076960711591E-5 0.0 50-51 2.020131876734078E-4 1.1363241806629188E-4 0.0 8.838076960711591E-5 0.0 52-53 2.209519240177898E-4 1.1363241806629188E-4 0.0 8.838076960711591E-5 0.0 54-55 2.9039395728052374E-4 1.1363241806629188E-4 0.0 1.010065938367039E-4 0.0 56-57 3.8508763900243364E-4 1.262582422958799E-4 0.0 1.073195059514979E-4 0.0 58-59 4.987200570687255E-4 1.262582422958799E-4 0.0 1.1363241806629188E-4 0.0 60-61 5.934137387906354E-4 1.3257115441067386E-4 0.0 1.1994533018108589E-4 0.0 62-63 7.322978053161033E-4 1.3888406652546787E-4 0.0 1.262582422958799E-4 0.0 64-65 8.459302233823952E-4 1.4519697864026187E-4 0.0 1.262582422958799E-4 0.0 66-67 9.72188465678275E-4 1.5150989075505585E-4 0.0 1.262582422958799E-4 0.0 68-69 0.001117385444318537 1.7044862709943783E-4 0.0 1.6413571498464385E-4 0.0 70-71 0.0012752082471883868 1.7676153921423183E-4 0.0 1.6413571498464385E-4 0.0 72-73 0.0015150989075505585 1.7676153921423183E-4 0.0 1.7044862709943783E-4 0.0 74-75 0.0017423637436831422 1.957002755586138E-4 0.0 1.7676153921423183E-4 0.0 76-77 0.0020580093494228418 2.020131876734078E-4 0.0 1.893873634438198E-4 0.0 78-79 0.002398906603621718 2.020131876734078E-4 0.0 1.893873634438198E-4 0.0 80-81 0.0027966200668537392 2.083260997882018E-4 0.0 1.893873634438198E-4 0.0 82-83 0.0033205917723816408 2.146390119029958E-4 0.0 1.893873634438198E-4 0.0 84-85 0.004065515401927332 2.146390119029958E-4 0.0 2.020131876734078E-4 0.0 86-87 0.005145023373557106 2.146390119029958E-4 0.0 2.020131876734078E-4 0.0 88-89 0.006458109093434256 2.146390119029958E-4 0.0 2.020131876734078E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 305 0.0 31.1322 3 CAACGCA 5565 0.0 20.090797 12-13 ATCAACG 5920 0.0 19.287004 10-11 TCAACGC 5525 0.0 18.77546 10-11 CGTACGC 275 0.0 18.127316 20-21 CGCGTAG 265 0.0 17.916605 54-55 ACTCGCG 280 0.0 17.805973 50-51 AACGCAG 5850 0.0 17.529491 12-13 CTCGCGT 285 0.0 17.493038 52-53 CGTAGCG 315 0.0 17.333055 56-57 ACGTACG 305 0.0 16.344301 20-21 GGTATCA 7190 0.0 16.177395 7 ACGCAGA 7240 0.0 15.573867 14-15 GTATCAA 7510 0.0 15.424863 8 AGTGGTA 7645 0.0 15.028564 4 TATCAAC 8015 0.0 14.571458 9 TGGTATC 8365 0.0 14.018529 6 CGCTAGT 370 0.0 13.473685 90-91 CGCAGAG 7715 0.0 13.41504 14-15 GTGGTAT 9010 0.0 13.06784 5 >>END_MODULE