FastQCFastQC Report
Sat 18 Jun 2016
SRR578566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8119591
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108440313.355389452498285No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT251080.3092273982765881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACACACAGCAGCAGCAG114970.14159580205456163No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG101040.12443976550050365No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA85560.10537476579793242No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC84690.10430328325650887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84240.10374906814887598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2413250.091.779795
GGTATCA2420000.091.7148068
GTATCAA2425450.091.560179
TGGTATC2426350.091.394577
AAGCAGT2435150.091.318931
GTGGTAT2433450.091.173616
AGCAGTG2454500.090.568052
GCAGTGG2449750.090.505143
CAGTGGT2447650.090.467724
TCAACGC2417100.046.4306112-13
AACGCAG2423400.046.2835514-15
CAACGCA2416650.046.28309612-13
ATCAACG2420800.046.2117410-11
CGCAGAG2429650.046.14517216-17
ACGCAGA2426200.046.11666514-15
TATCAAC2437400.045.9788110-11
CAGAGTA2416100.045.84970518-19
AGAGTAC2378600.045.72151618-19
GAGTACT1348900.045.46935320-21
GCAGAGT2455200.045.3991516-17
Produced by FastQC (version 0.11.5)