##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578566_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8119591
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.062766215687464	31.0	31.0	34.0	28.0	34.0
2	31.4147240913982	33.0	31.0	34.0	28.0	34.0
3	31.53910621852751	34.0	31.0	34.0	28.0	34.0
4	34.92736813960211	37.0	35.0	37.0	32.0	37.0
5	34.954228975326465	37.0	35.0	37.0	32.0	37.0
6	35.00401682794121	37.0	35.0	37.0	32.0	37.0
7	34.92207181371574	37.0	35.0	37.0	32.0	37.0
8	34.96897848672427	37.0	35.0	37.0	32.0	37.0
9	36.470456085780675	39.0	37.0	39.0	32.0	39.0
10-11	36.3477494125012	39.0	37.0	39.0	32.0	39.0
12-13	36.418680448313225	39.0	37.0	39.0	32.0	39.0
14-15	37.69273969587877	40.0	38.0	41.0	32.0	41.0
16-17	37.66115306793162	40.0	38.0	41.0	32.0	41.0
18-19	37.58136647523256	40.0	38.0	41.0	32.0	41.0
20-21	37.500531861764955	40.0	38.0	41.0	31.5	41.0
22-23	37.229149411589816	39.5	37.5	41.0	31.5	41.0
24-25	37.19156882409471	40.0	37.0	41.0	31.0	41.0
26-27	37.086960291472806	40.0	37.0	41.0	30.0	41.0
28-29	36.9347707292153	40.0	37.0	41.0	30.0	41.0
30-31	36.69383464019308	40.0	36.0	41.0	30.0	41.0
32-33	36.39328661997877	39.5	36.0	41.0	29.0	41.0
34-35	36.233037045831495	39.0	36.0	41.0	28.5	41.0
36-37	35.946483511300016	39.0	35.0	40.5	27.5	41.0
38-39	35.021626828247875	38.0	35.0	40.0	26.5	41.0
40-41	34.78589592751655	37.5	34.0	40.0	26.0	41.0
42-43	34.483643695846254	37.0	34.0	40.0	24.5	41.0
44-45	34.20110532661066	37.0	33.5	40.0	24.0	41.0
46-47	34.048549797643744	37.0	33.0	40.0	23.5	41.0
48-49	34.040163907270696	37.0	33.0	40.0	23.0	41.0
50-51	33.10945452794358	36.5	32.0	39.5	20.0	40.5
52-53	32.64265829399535	37.0	33.0	39.5	11.0	40.5
54-55	32.17749742567082	38.0	31.5	40.0	2.0	41.0
56-57	31.618783076635264	37.0	31.0	40.0	2.0	41.0
58-59	31.308133993448685	37.0	31.0	40.0	2.0	41.0
60-61	31.03932537981285	36.5	30.5	40.0	2.0	41.0
62-63	30.696618277940352	36.0	30.0	40.0	2.0	41.0
64-65	30.392986666446625	35.5	29.5	39.0	2.0	41.0
66-67	30.04180653927027	35.0	29.0	39.0	2.0	41.0
68-69	29.696353547857278	35.0	29.0	38.5	2.0	40.5
70-71	29.390299708445905	35.0	29.0	37.5	2.0	40.0
72-73	29.06605954659539	35.0	28.5	37.0	2.0	39.5
74-75	28.709627184423454	35.0	28.0	36.5	2.0	39.0
76-77	28.339433291652252	35.0	27.0	36.0	2.0	39.0
78-79	27.99904102312543	35.0	26.5	36.0	2.0	37.5
80-81	27.682702613961713	34.0	26.0	35.0	2.0	37.0
82-83	27.414273452936236	34.0	26.0	35.0	2.0	37.0
84-85	27.144729888488225	34.0	25.5	35.0	2.0	36.0
86-87	26.90905065292082	34.0	25.0	35.0	2.0	36.0
88-89	26.663941324138122	34.0	25.0	35.0	2.0	36.0
90-91	26.429308200376102	34.0	24.0	35.0	2.0	35.0
92-93	26.14456442448887	34.0	23.0	35.0	2.0	35.0
94-95	25.864571257345354	34.0	20.0	35.0	2.0	35.0
96-97	25.593256421413344	34.0	18.5	35.0	2.0	35.0
98-99	25.286105359247777	34.0	8.5	35.0	2.0	35.0
100-101	24.44275099570902	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6145280072061965
1101	2	0.6290239781728957
1101	3	0.5197244171415214
1101	4	0.61843617339931
1101	5	0.5766336842072448
1101	6	0.5197700135199526
1101	7	0.5161696713411104
1101	8	0.5271657176249818
1101	9	0.6250760256685481
1101	10-11	0.7341288875078789
1101	12-13	0.7457023287862583
1101	14-15	0.8808726362966794
1101	16-17	0.822893620443061
1101	18-19	0.8347139644927353
1101	20-21	0.890099044200241
1101	22-23	0.8711582067479
1101	24-25	0.8460230996493863
1101	26-27	0.8922782636880342
1101	28-29	0.9502016584621984
1101	30-31	1.0339237448349579
1101	32-33	1.0824514891180215
1101	34-35	1.1022735480037866
1101	36-37	1.1064274232639661
1101	38-39	1.145027472725566
1101	40-41	1.0751480272749987
1101	42-43	1.1556767990517187
1101	44-45	1.3285832841558758
1101	46-47	1.2221510382435454
1101	48-49	1.1571405762296436
1101	50-51	1.2874398589627063
1101	52-53	1.050728774907867
1101	54-55	1.0041184650631507
1101	56-57	0.9924884440469448
1101	58-59	1.0332608047755976
1101	60-61	0.9702004851461474
1101	62-63	0.9718052891452587
1101	64-65	1.0410073633464485
1101	66-67	1.0227250344678929
1101	68-69	0.982627143411861
1101	70-71	1.035428632389145
1101	72-73	0.958928721011258
1101	74-75	0.9398810816026497
1101	76-77	0.9176019053433428
1101	78-79	0.9240014915861217
1101	80-81	0.896484089295928
1101	82-83	0.955569551352184
1101	84-85	0.9204131800470208
1101	86-87	0.9344723375177715
1101	88-89	0.905067890181396
1101	90-91	0.9700578833402922
1101	92-93	1.0566782289923928
1101	94-95	1.1120726697932852
1101	96-97	1.1234741339115324
1101	98-99	1.0581989092288602
1101	100-101	1.1931743278797917
1102	1	0.5309213687805503
1102	2	0.46265330265767446
1102	3	0.5038680519514678
1102	4	0.5131979051072975
1102	5	0.5765078708348526
1102	6	0.5824477986046048
1102	7	0.5954855432717778
1102	8	0.5195068314764129
1102	9	0.6639759096176263
1102	10-11	0.675426854797216
1102	12-13	0.6674638839516618
1102	14-15	0.8126295032865656
1102	16-17	0.8094589715538163
1102	18-19	0.8833228775087889
1102	20-21	0.8917650482289687
1102	22-23	0.9540926783706425
1102	24-25	0.9631473050268013
1102	26-27	1.0140756331553646
1102	28-29	1.0967765558064073
1102	30-31	1.064455006722497
1102	32-33	1.130773264173051
1102	34-35	1.065866246418956
1102	36-37	1.0776548864552211
1102	38-39	1.125919951924864
1102	40-41	1.1703182798602256
1102	42-43	1.1590296659670827
1102	44-45	1.2172243821677
1102	46-47	1.3224772858392484
1102	48-49	1.2158828865165958
1102	50-51	1.1998986858840865
1102	52-53	1.1113966232225607
1102	54-55	1.1215732384549781
1102	56-57	1.0071276982554203
1102	58-59	1.0710719895442544
1102	60-61	1.046267286386719
1102	62-63	1.0801369424766385
1102	64-65	1.0898259884822714
1102	66-67	1.0413821815417599
1102	68-69	0.9554728118977138
1102	70-71	0.9833463826594837
1102	72-73	1.0048555516897473
1102	74-75	0.9025990844186431
1102	76-77	1.0072108505019237
1102	78-79	1.0101843852570056
1102	80-81	1.0156629047270833
1102	82-83	1.03297047587931
1102	84-85	1.0432716061231204
1102	86-87	1.0102906451858296
1102	88-89	1.0717870736801807
1102	90-91	1.049721550906657
1102	92-93	1.0166788853562707
1102	94-95	1.10481762117265
1102	96-97	1.2072264194815538
1102	98-99	1.2405316785718803
1102	100-101	1.3164192538250497
1103	1	0.4460179095973942
1103	2	0.47739243547377797
1103	3	0.3530748466137865
1103	4	0.46512420877241567
1103	5	0.4765326391595295
1103	6	0.5060634526345424
1103	7	0.3845240373857024
1103	8	0.3844240850053424
1103	9	0.5229972748546032
1103	10-11	0.5456452939030001
1103	12-13	0.5757018093876027
1103	14-15	0.6532105024837378
1103	16-17	0.763784468374574
1103	18-19	0.7412623021675913
1103	20-21	0.7284303953087488
1103	22-23	0.8257448637191303
1103	24-25	0.7830082457280625
1103	26-27	0.8403196113426858
1103	28-29	0.8650466649135993
1103	30-31	0.9318669672253179
1103	32-33	0.9989860788679081
1103	34-35	0.9512106617377114
1103	36-37	1.0116920539465752
1103	38-39	0.8874848622279004
1103	40-41	0.9519475455547521
1103	42-43	0.8584299229998962
1103	44-45	0.7769479442710789
1103	46-47	1.0319606184784575
1103	48-49	1.0941514340091985
1103	50-51	0.9806566826667691
1103	52-53	0.8478165623781564
1103	54-55	0.9003013309523666
1103	56-57	0.9334034249421741
1103	58-59	0.9547448118452913
1103	60-61	0.9815269559613746
1103	62-63	0.79461253032553
1103	64-65	0.7204374098839814
1103	66-67	0.7351397599187486
1103	68-69	0.9425106455204109
1103	70-71	0.9054431374036866
1103	72-73	0.9930211041326196
1103	74-75	1.0066297721751525
1103	76-77	1.022544103902117
1103	78-79	0.9830634282623265
1103	80-81	1.0853207096598183
1103	82-83	1.0516539779903482
1103	84-85	1.0519880231930259
1103	86-87	1.0687490631464591
1103	88-89	1.0785741216448095
1103	90-91	0.9911196222341836
1103	92-93	1.0334204138765983
1103	94-95	1.028294317449081
1103	96-97	0.9673225727957693
1103	98-99	1.0745583530556004
1103	100-101	1.070205791407293
1104	1	0.3666132367067263
1104	2	0.3540748760445034
1104	3	0.38610956513687
1104	4	0.36416331279603753
1104	5	0.4401516902223932
1104	6	0.4372966341641984
1104	7	0.42843095432183986
1104	8	0.34417607781387716
1104	9	0.5293488519779217
1104	10-11	0.5008655480359323
1104	12-13	0.5098048915698286
1104	14-15	0.6134873922783939
1104	16-17	0.6052181128075276
1104	18-19	0.6724825229255487
1104	20-21	0.6541981147210905
1104	22-23	0.6475249332002804
1104	24-25	0.6814536767815227
1104	26-27	0.7100074584328411
1104	28-29	0.7070375026809899
1104	30-31	0.8439754543289979
1104	32-33	0.7897627268879575
1104	34-35	0.8815785248496795
1104	36-37	0.8973685966413711
1104	38-39	0.8872735006339454
1104	40-41	0.8488061067476949
1104	42-43	0.9997040066933067
1104	44-45	0.9476240664684497
1104	46-47	1.1142686469066447
1104	48-49	0.9985564801426889
1104	50-51	0.9940776901703927
1104	52-53	0.9558201910289768
1104	54-55	0.9325826753716697
1104	56-57	0.9268270403516645
1104	58-59	0.9013421064808007
1104	60-61	0.904200692052715
1104	62-63	0.7522681482738953
1104	64-65	0.69210912936828
1104	66-67	0.7743335843958192
1104	68-69	0.7450799578555376
1104	70-71	0.7323079841428779
1104	72-73	0.7246758423236663
1104	74-75	0.7922349695598889
1104	76-77	0.8084514111110046
1104	78-79	0.6766282618661066
1104	80-81	0.8063427183856184
1104	82-83	0.7753054139753672
1104	84-85	0.8123427609411955
1104	86-87	0.8491476885444733
1104	88-89	0.8802007104779044
1104	90-91	0.8658301992420299
1104	92-93	0.7527050628304153
1104	94-95	0.6293956189319942
1104	96-97	0.7598412147342657
1104	98-99	1.0154779907466178
1104	100-101	0.9632930590967312
1105	1	0.193006670913217
1105	2	0.15929159035458795
1105	3	0.18427663982209452
1105	4	0.1891752335154706
1105	5	0.13354255725024444
1105	6	0.11765556953385925
1105	7	0.09052264179049274
1105	8	0.10504538053971402
1105	9	0.04290906762086877
1105	10-11	0.0916761796288057
1105	12-13	0.07693064709219044
1105	14-15	0.004938874731472254
1105	16-17	-0.018904167680375394
1105	18-19	-0.12439368933352313
1105	20-21	7.18670839667368E-4
1105	22-23	-0.03489269653324101
1105	24-25	-0.09407747590996962
1105	26-27	-0.3606873402564119
1105	28-29	-0.15502054411636124
1105	30-31	-0.3411659939697955
1105	32-33	-0.3372106398622705
1105	34-35	-0.17967929410699668
1105	36-37	-0.1309248727529564
1105	38-39	-0.09695221572592772
1105	40-41	-0.09823677245784523
1105	42-43	-0.037599305740684485
1105	44-45	-0.04009175071162474
1105	46-47	0.005350336309952297
1105	48-49	-0.005856915929257411
1105	50-51	-0.06649155845860832
1105	52-53	-0.10689722282656078
1105	54-55	-0.1981486756706019
1105	56-57	0.0022036763987500763
1105	58-59	6.245595964813333E-4
1105	60-61	-0.035842560669422596
1105	62-63	0.01412140808109541
1105	64-65	-0.08399483019602272
1105	66-67	-0.1057110024883876
1105	68-69	0.003565062885932946
1105	70-71	3.938958434162032E-4
1105	72-73	-0.027467125008609372
1105	74-75	-0.0031453522211215557
1105	76-77	0.03877240579842223
1105	78-79	0.07700926392914909
1105	80-81	0.11666232280670741
1105	82-83	0.1375882652160243
1105	84-85	0.10242036962802104
1105	86-87	0.1303284411315495
1105	88-89	0.19331076444230533
1105	90-91	0.2295333975131939
1105	92-93	0.2905626325780162
1105	94-95	0.2917811578204734
1105	96-97	0.2927921713785473
1105	98-99	0.282139952045501
1105	100-101	0.23316791094956812
1106	1	-0.1753991844326528
1106	2	-0.11681478905844145
1106	3	-0.16779788456763356
1106	4	-0.17028188078281659
1106	5	-0.24221503842111503
1106	6	-0.19207382845233667
1106	7	-0.23922198993407306
1106	8	-0.25362830438690054
1106	9	-0.1776369276742642
1106	10-11	-0.3772371194181048
1106	12-13	-0.3949122042995512
1106	14-15	-0.3941868640709245
1106	16-17	-0.4023650339719822
1106	18-19	-0.4372720526968692
1106	20-21	-0.21083518695179748
1106	22-23	-0.22158852571448762
1106	24-25	-0.2903652044185421
1106	26-27	-0.2864697870899917
1106	28-29	-0.34593998817614136
1106	30-31	-0.3357560116428999
1106	32-33	-0.2937744465541954
1106	34-35	-0.29755691872814793
1106	36-37	-0.3433005212432505
1106	38-39	-0.391560603921171
1106	40-41	-0.4264506283457976
1106	42-43	-0.4912154654002734
1106	44-45	-0.4487741724223113
1106	46-47	-0.500001024602021
1106	48-49	-0.7038630435271997
1106	50-51	-0.8224819720710599
1106	52-53	-0.9149689462956019
1106	54-55	-0.9423215859817766
1106	56-57	-0.946915864150089
1106	58-59	-0.9149904992076827
1106	60-61	-1.068352278492835
1106	62-63	-0.9670313934353203
1106	64-65	-0.9147214906019414
1106	66-67	-1.075317960159932
1106	68-69	-1.1601666783385411
1106	70-71	-1.0542715538923169
1106	72-73	-1.1147846324195605
1106	74-75	-1.2174063043333412
1106	76-77	-1.261444889990024
1106	78-79	-1.139718242896027
1106	80-81	-1.1450506966552503
1106	82-83	-1.2388130112484532
1106	84-85	-1.2558733612587396
1106	86-87	-1.2165394536385499
1106	88-89	-1.3250695986387129
1106	90-91	-1.2378120747483372
1106	92-93	-1.2388379963203988
1106	94-95	-1.2522330548579532
1106	96-97	-1.1919973953337362
1106	98-99	-1.2885389816279336
1106	100-101	-1.4193098306274408
1107	1	-0.4375683315672667
1107	2	-0.5301878236768616
1107	3	-0.46177369881209174
1107	4	-0.3587864954774673
1107	5	-0.46135573295296695
1107	6	-0.44790840146757915
1107	7	-0.4216706276266393
1107	8	-0.37022782578748803
1107	9	-0.4569886015020117
1107	10-11	-0.4447104061823808
1107	12-13	-0.5214052094366579
1107	14-15	-0.7504144340725745
1107	16-17	-0.6371736075978589
1107	18-19	-0.6018498636624585
1107	20-21	-0.4799977866768401
1107	22-23	-0.5474848845683127
1107	24-25	-0.5996000252503748
1107	26-27	-0.4534437312018227
1107	28-29	-0.5448075419251452
1107	30-31	-0.5067413908426772
1107	32-33	-0.4409094166451766
1107	34-35	-0.44486138677333287
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2108	10-11	-1.7580206571992179
2108	12-13	-1.2960242799044934
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2108	18-19	-1.2472739293885269
2108	20-21	-1.269310246750301
2108	22-23	-1.2393539986693938
2108	24-25	-1.3281993655834796
2108	26-27	-1.4575775223123841
2108	28-29	-1.6997446083815433
2108	30-31	-1.9407520020526619
2108	32-33	-1.891847361979643
2108	34-35	-2.604435791765546
2108	36-37	-2.4170196123311385
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2208	8	-0.6251721951470373
2208	9	-0.8178456643103331
2208	10-11	-1.0147061646333384
2208	12-13	-0.930478865754857
2208	14-15	-1.2205335823867003
2208	16-17	-1.0503168910085705
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2208	22-23	-1.0599070188734672
2208	24-25	-1.0251769228226593
2208	26-27	-1.0592066527098751
2208	28-29	-1.060451392371185
2208	30-31	-1.5415040034862244
2208	32-33	-1.4492256999326187
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2308	54-55	-2.2275326306601997
2308	56-57	-2.164627634064235
2308	58-59	-2.0633018898611937
2308	60-61	-2.1074402945001403
2308	62-63	-2.140245652700539
2308	64-65	-2.102694831240619
2308	66-67	-2.3187138515423307
2308	68-69	-2.0882662623590704
2308	70-71	-2.030254526392607
2308	72-73	-2.004049647153881
2308	74-75	-1.8641911257028703
2308	76-77	-1.7741483471938047
2308	78-79	-1.8198745480033267
2308	80-81	-2.376560828295837
2308	82-83	-2.185836831949487
2308	84-85	-1.9432004302659145
2308	86-87	-1.8746543811741638
2308	88-89	-1.8060061123622937
2308	90-91	-2.023746268213401
2308	92-93	-2.0825683467085625
2308	94-95	-2.173586177381175
2308	96-97	-2.084006225764174
2308	98-99	-2.094812825243377
2308	100-101	-2.2789843805955137
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67845.0
3	12400.0
4	10830.0
5	10615.0
6	12288.0
7	15330.0
8	18501.0
9	21661.0
10	25056.0
11	30061.0
12	29675.0
13	33226.0
14	36748.0
15	43616.0
16	55509.0
17	75993.0
18	118394.0
19	195895.0
20	285448.0
21	200671.0
22	103988.0
23	88105.0
24	91111.0
25	95122.0
26	102391.0
27	113774.0
28	129893.0
29	151277.0
30	177288.0
31	212721.0
32	261244.0
33	327212.0
34	432415.0
35	634322.0
36	1054915.0
37	1393442.0
38	1230808.0
39	219575.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.49273572914722	49.11869463076704	23.306008757418763	17.08256088266698
2	16.375166096707947	50.72227027865293	17.234042789248658	15.668520835390472
3	46.157609467385605	19.74647024135877	18.184210604166093	15.911709687089534
4	16.444095138669134	18.14581478775977	19.076999742703272	46.33309033086782
5	16.3534248405748	48.09624112273996	20.131161694267004	15.419172342418234
6	46.25862170254479	19.32348877963998	18.04671998124018	16.37116953657506
7	17.20091695199796	18.699837637965548	48.83366715893781	15.26557825109868
8	45.90217278582491	19.45783442666038	18.93605003612401	15.703942751390706
9	45.51087663945269	19.128294732713968	19.45644392789734	15.904384699936
10-11	16.01909702431617	33.827011229968164	33.95715270986746	16.19673903584821
12-13	15.806825678924266	19.43689085033241	33.916644207076274	30.839639263667053
14-15	16.00508135945765	48.85126529287009	18.966284415097086	16.177368932575174
16-17	30.74461282438702	19.261619111005796	19.103646938444736	30.89012112616245
18-19	16.157861535608024	33.936343238895375	19.080204730658586	30.82559049483801
20-21	30.942484687180148	33.96214622039512	19.10014372039065	15.995225372034078
22-23	30.8286944365383	19.213684670623447	33.900188273586515	16.057432619251742
24-25	16.081815779082888	33.712913819931046	19.57637593487508	30.628894466110985
26-27	16.31572202713951	25.301858534103832	42.083157804051865	16.29926163470479
28-29	28.663921254109255	19.09410745806014	36.06246406791367	16.179507219916932
30-31	26.356862113338575	20.306566568726563	36.48470892870867	16.851862389226195
32-33	20.061455164295484	22.893400371300086	38.10502220681912	18.94012225758531
34-35	19.111859467379738	21.97974745623905	38.88980678371971	20.018586292661507
36-37	18.964973216522377	22.153028526922277	39.01738247328241	19.86461578327293
38-39	19.38525152468125	22.270174576896178	38.727544220621176	19.617029677801394
40-41	19.670598279555776	21.944343742019456	38.623861386059346	19.76119659236542
42-43	20.015290336267316	22.26506964862093	38.61007579585338	19.109564219258374
44-45	19.495237850091883	22.39606470656553	38.41710011654591	19.691597326796682
46-47	19.749553852387645	22.16625141172312	38.27221716581419	19.811977570075044
48-49	19.759892696656646	22.260080056294722	38.44965241631121	19.530374830737422
50-51	19.844026704602733	22.30637109263888	37.558244889983705	20.291357312774682
52-53	20.325239145160793	23.186391839079285	36.396469744001315	20.091899271758606
54-55	20.900434327029902	24.325662146455628	34.46127711090632	20.312626415608147
56-57	21.47829007391475	24.905742184458056	33.26549742520123	20.350470316425966
58-59	21.280269936521538	25.885407068533546	32.26908668118785	20.56523631375706
60-61	21.370733161277357	26.199649661729076	31.720020355744687	20.709596821248883
62-63	21.278939608040805	25.98272616645415	32.152865726852184	20.585468498652865
64-65	21.004771712822283	25.927763349085325	32.062089047106845	21.005375890985544
66-67	21.02188668514824	25.440477700312574	32.856792563357175	20.68084305118201
68-69	20.81854128286265	25.179249683234506	33.160124619505254	20.842084414397593
70-71	20.684692932138944	24.98688423979796	33.71660105344109	20.61182177462201
72-73	20.41893861059742	24.837013120083174	33.47516362805084	21.26888464126857
74-75	20.909713503306264	24.8816858003971	32.87588464371704	21.332716052579595
76-77	21.358021151561736	25.488726371131815	32.27870264090841	20.87454983639804
78-79	21.16371516600187	25.47240661392694	32.05363023481266	21.31024798525853
80-81	21.135531686660777	25.715916796015666	31.922757127843017	21.22579438948054
82-83	22.19896200779354	25.950002444459745	31.198963425765147	20.65207212198156
84-85	21.577272202925244	26.58320556882473	30.757931310569457	21.08159091768057
86-87	21.491039804853244	26.33500908615714	30.796301760792073	21.377649348197547
88-89	21.422415807458865	26.080091315631414	30.634016996278497	21.863475880631224
90-91	21.46533459102294	26.094706049994272	30.83960276969593	21.600356589286857
92-93	21.489245762787125	26.489240829198163	30.786483150313625	21.235030257701084
94-95	21.66593889425467	26.250275998435907	30.226207701312337	21.85757740599709
96-97	21.43410022718913	26.17013256111891	30.930446673710872	21.465320537981093
98-99	21.646598089040083	26.442441992328586	30.309075376149398	21.60188454248194
100-101	22.01185270776999	26.267870702329816	30.107215735867683	21.613060854032515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9328.0
1	5558.0
2	1288.0
3	694.5
4	560.0
5	515.5
6	645.0
7	831.5
8	776.0
9	806.0
10	1342.5
11	8654.0
12	156772.0
13	173267.5
14	43554.5
15	40092.0
16	44180.5
17	48970.5
18	52230.0
19	54285.5
20	56245.5
21	58051.5
22	59909.0
23	61923.0
24	64703.0
25	68546.5
26	73928.5
27	80507.5
28	87198.0
29	93376.5
30	99394.5
31	106281.5
32	114236.0
33	125147.0
34	144498.5
35	155357.0
36	158400.0
37	167183.0
38	175918.0
39	185416.0
40	194959.0
41	202177.5
42	207859.5
43	218237.5
44	231638.0
45	239529.0
46	243294.5
47	252053.0
48	259567.0
49	262533.0
50	265225.0
51	265695.5
52	266462.5
53	272852.5
54	270011.0
55	255851.5
56	253955.0
57	241270.5
58	227544.0
59	194519.5
60	139211.5
61	106824.0
62	82136.0
63	63053.0
64	46182.0
65	29767.0
66	17766.5
67	10318.5
68	6119.0
69	3647.5
70	2395.5
71	1724.5
72	1365.5
73	1127.0
74	987.5
75	878.5
76	745.0
77	657.0
78	580.5
79	514.5
80	456.0
81	377.0
82	312.0
83	260.5
84	214.5
85	178.5
86	139.0
87	113.0
88	98.0
89	79.0
90	62.5
91	49.5
92	33.0
93	23.0
94	21.5
95	11.5
96	7.0
97	4.5
98	3.5
99	3.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.051554320901138984
2	0.09360077373355383
3	0.0844254347294094
4	0.05476876852540971
5	0.08632208198664194
6	0.0013547480408803842
7	0.0010099030850199227
8	0.004150455361606268
9	0.008904389395968344
10-11	0.009046022145696749
12-13	0.01609686990391511
14-15	0.011053512424455863
16-17	0.0015148546275298841
18-19	5.542151076328844E-4
20-21	6.77374020440192E-5
22-23	2.9558139073753835E-4
24-25	7.020058030016536E-4
26-27	0.001237747073713442
28-29	0.001317800367038192
30-31	0.004224350709290652
32-33	1.6010658664949995E-4
34-35	0.0020505958982416726
36-37	4.0642441226411527E-4
38-39	0.002327703452058115
40-41	0.0028880765053313647
42-43	0.001028376921941019
44-45	5.418992163521536E-4
46-47	9.97587193739192E-4
48-49	0.0022107024848911725
50-51	0.0038979795903512876
52-53	0.13059771114086904
54-55	0.14058589896954168
56-57	0.12705689239765894
58-59	0.12109600101778525
60-61	0.10910032291035349
62-63	0.11369415035806607
64-65	0.11577553598450957
66-67	0.12403949903387991
68-69	0.13632460058640886
70-71	0.12405181492516064
72-73	0.11612653888601039
74-75	0.12105289539830269
76-77	0.1321926190617237
78-79	0.1459556275679403
80-81	0.13593049206542546
82-83	0.11594795846243978
84-85	0.1224753808412271
86-87	0.12318354458986912
88-89	0.11943951364052696
90-91	0.12983412588146373
92-93	0.1465960539145383
94-95	0.15593149950533222
96-97	0.15627634446119268
98-99	0.13929273038506496
100-101	0.12899048732873367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8119591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.64102522810285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.35669544310062	40.78256546031563
2	4.7961226815083755	4.282076672445833
3	1.2935710174561315	1.732390092738055
4	0.6441483886720439	1.1502177787740204
5	0.3514289964363457	0.7844075347900886
6	0.25051784269588395	0.6710024001526115
7	0.1711247542285004	0.534742912946717
8	0.13513746368490953	0.48261399404959027
9	0.10398746249831482	0.41778962431143035
>10	0.8416350030600468	7.127115685261723
>50	0.03293477647863438	1.0451368337730877
>100	0.015266998610305539	1.5205924631893073
>500	0.0028522712277198638	0.8555574218187627
>1k	0.0037145857849371824	3.225166916285196
>5k	5.969870011506185E-4	1.895000614913993
>10k+	2.653275560669416E-4	33.49362359423396
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1084403	13.355389452498285	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	25108	0.3092273982765881	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACACACAGCAGCAGCAG	11497	0.14159580205456163	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	10104	0.12443976550050365	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	8556	0.10537476579793242	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	8469	0.10430328325650887	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8424	0.10374906814887598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	2.4631782561461533E-5	0.0	0.0	0.0
2	0.0	2.4631782561461533E-5	0.0	0.0	0.0
3	0.0	3.6947673842192294E-5	0.0	0.0	0.0
4	0.0	3.6947673842192294E-5	0.0	0.0	0.0
5	0.0	3.6947673842192294E-5	0.0	1.2315891280730766E-5	0.0
6	0.0	4.9263565122923066E-5	0.0	1.2315891280730766E-5	0.0
7	0.0	4.9263565122923066E-5	0.0	1.2315891280730766E-5	0.0
8	0.0	4.9263565122923066E-5	0.0	2.4631782561461533E-5	0.0
9	0.0	4.9263565122923066E-5	0.0	2.4631782561461533E-5	0.0
10-11	1.2315891280730766E-5	6.157945640365383E-5	0.0	2.4631782561461533E-5	0.0
12-13	1.2315891280730766E-5	6.157945640365383E-5	0.0	2.4631782561461533E-5	0.0
14-15	1.2315891280730766E-5	6.157945640365383E-5	0.0	2.4631782561461533E-5	0.0
16-17	1.847383692109615E-5	6.157945640365383E-5	0.0	2.4631782561461533E-5	0.0
18-19	2.4631782561461533E-5	6.157945640365383E-5	0.0	2.4631782561461533E-5	0.0
20-21	2.4631782561461533E-5	7.38953476843846E-5	0.0	2.4631782561461533E-5	0.0
22-23	3.6947673842192294E-5	9.852713024584613E-5	0.0	4.310561948255768E-5	0.0
24-25	3.6947673842192294E-5	9.852713024584613E-5	0.0	5.5421510763288445E-5	0.0
26-27	3.6947673842192294E-5	1.0468507588621152E-4	0.0	6.157945640365383E-5	0.0
28-29	3.6947673842192294E-5	1.1084302152657689E-4	0.0	6.157945640365383E-5	0.0
30-31	3.6947673842192294E-5	1.1084302152657689E-4	0.0	7.389534768438459E-5	0.0
32-33	3.6947673842192294E-5	1.1700096716694228E-4	0.0	8.005329332474997E-5	0.0
34-35	4.310561948255768E-5	1.2931685844767306E-4	0.0	8.621123896511536E-5	0.0
36-37	4.9263565122923066E-5	1.3547480408803843E-4	0.0	8.621123896511536E-5	0.0
38-39	4.9263565122923066E-5	1.3547480408803843E-4	0.0	8.621123896511536E-5	6.157945640365383E-6
40-41	4.9263565122923066E-5	1.3547480408803843E-4	0.0	8.621123896511536E-5	1.2315891280730766E-5
42-43	7.38953476843846E-5	1.3547480408803843E-4	0.0	8.621123896511536E-5	1.2315891280730766E-5
44-45	9.852713024584613E-5	1.4779069536876918E-4	0.0	8.621123896511536E-5	1.2315891280730766E-5
46-47	1.1084302152657689E-4	1.5394864100913455E-4	0.0	8.621123896511536E-5	1.2315891280730766E-5
48-49	1.477906953687692E-4	1.7242247793023072E-4	0.0	9.852713024584613E-5	2.4631782561461533E-5
50-51	1.7242247793023072E-4	1.9705426049169226E-4	0.0	9.852713024584613E-5	2.4631782561461533E-5
52-53	2.0321220613205764E-4	1.9705426049169226E-4	0.0	9.852713024584613E-5	2.4631782561461533E-5
54-55	2.4015987997424992E-4	1.9705426049169226E-4	0.0	9.852713024584613E-5	2.4631782561461533E-5
56-57	3.140552276586345E-4	2.0321220613205764E-4	0.0	9.852713024584613E-5	2.4631782561461533E-5
58-59	4.0642441226411527E-4	2.09370151772423E-4	0.0	1.1084302152657689E-4	2.4631782561461533E-5
60-61	4.8647770558886525E-4	2.09370151772423E-4	0.0	1.1084302152657689E-4	2.4631782561461533E-5
62-63	5.973207271154421E-4	2.09370151772423E-4	0.0	1.1084302152657689E-4	2.4631782561461533E-5
64-65	7.08163748642019E-4	2.09370151772423E-4	0.0	1.1084302152657689E-4	2.4631782561461533E-5
66-67	8.744282809318843E-4	2.09370151772423E-4	0.0	1.1700096716694228E-4	2.4631782561461533E-5
68-69	0.001016061030660288	2.2168604305315378E-4	0.0	1.2315891280730766E-4	2.4631782561461533E-5
70-71	0.001176167617309788	2.2168604305315378E-4	0.0	1.2931685844767306E-4	2.4631782561461533E-5
72-73	0.0013793798234418458	2.3400193433388455E-4	0.0	1.3547480408803843E-4	2.4631782561461533E-5
74-75	0.0015702761382931727	2.3400193433388455E-4	0.0	1.3547480408803843E-4	2.4631782561461533E-5
76-77	0.0017611724531444994	2.3400193433388455E-4	0.0	1.3547480408803843E-4	2.4631782561461533E-5
78-79	0.0020567538438820377	2.463178256146153E-4	0.0	1.3547480408803843E-4	2.4631782561461533E-5
80-81	0.0023092296151370185	2.463178256146153E-4	0.0	1.3547480408803843E-4	2.4631782561461533E-5
82-83	0.002752601701243326	2.463178256146153E-4	0.0	1.4779069536876918E-4	2.4631782561461533E-5
84-85	0.003442291612964249	2.463178256146153E-4	0.0	1.4779069536876918E-4	2.4631782561461533E-5
86-87	0.004230508654931018	2.463178256146153E-4	0.0	1.4779069536876918E-4	2.4631782561461533E-5
88-89	0.00546825572864446	2.463178256146153E-4	0.0	1.4779069536876918E-4	2.4631782561461533E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	241325	0.0	91.77979	5
GGTATCA	242000	0.0	91.714806	8
GTATCAA	242545	0.0	91.56017	9
TGGTATC	242635	0.0	91.39457	7
AAGCAGT	243515	0.0	91.31893	1
GTGGTAT	243345	0.0	91.17361	6
AGCAGTG	245450	0.0	90.56805	2
GCAGTGG	244975	0.0	90.50514	3
CAGTGGT	244765	0.0	90.46772	4
TCAACGC	241710	0.0	46.43061	12-13
AACGCAG	242340	0.0	46.28355	14-15
CAACGCA	241665	0.0	46.283096	12-13
ATCAACG	242080	0.0	46.21174	10-11
CGCAGAG	242965	0.0	46.145172	16-17
ACGCAGA	242620	0.0	46.116665	14-15
TATCAAC	243740	0.0	45.97881	10-11
CAGAGTA	241610	0.0	45.849705	18-19
AGAGTAC	237860	0.0	45.721516	18-19
GAGTACT	134890	0.0	45.469353	20-21
GCAGAGT	245520	0.0	45.39915	16-17
>>END_MODULE