FastQCFastQC Report
Sat 18 Jun 2016
SRR578566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8119591
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA986121.2144946709754223No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223820.275654278645316No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97850.12051099618195056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3850.023.4034213
CAACGCA66050.020.52923212-13
ATCAACG68450.019.77480110-11
CGCGTAG2400.019.75460254-55
CTCGCGT2350.019.16616652-53
TCAACGC64900.017.7882710-11
GGTATCA80750.017.5584328
AGTGGTA81950.017.0699735
AACGCAG68500.017.06105412-13
CGTAGCG2650.016.99641256-57
GTATCAA84600.016.9826419
ACGCAGA84550.016.17751114-15
CGTACGC2650.016.10320320-21
TGGTATC93800.015.4694787
ACTCGCG3100.015.29388550-51
TCGCGTA3000.015.01349652-53
TATCAAC88250.014.7757999
GTGGTAT97950.014.7656466
AGAGTAC90650.014.30512718-19
ACGTACG3600.013.8293720-21
Produced by FastQC (version 0.11.5)