FastQCFastQC Report
Sat 18 Jun 2016
SRR578565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8160745
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116030914.21817493378362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC283400.3472722159557737No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT248940.3050456790403327No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG94770.11612910340906375No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT89300.10942628399735563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2521700.092.044925
GGTATCA2529600.091.830768
AAGCAGT2538050.091.797771
GTATCAA2530900.091.7161949
TGGTATC2535100.091.650277
GTGGTAT2550800.091.11286
GCAGTGG2554400.090.985343
AGCAGTG2566350.090.73822
CAGTGGT2553800.090.721634
TCAACGC2524850.046.49611712-13
AACGCAG2535200.046.37401214-15
CAACGCA2526050.046.36587512-13
ATCAACG2525950.046.33960310-11
CGCAGAG2545350.046.21254716-17
ACGCAGA2538350.046.1985514-15
GTATCGA6700.046.097489
TATCAAC2540500.046.0863710-11
CAGAGTA2527750.045.96289418-19
AGAGTAC2483700.045.88403318-19
GAGTACT1450150.045.82113620-21
Produced by FastQC (version 0.11.5)