FastQCFastQC Report
Sat 18 Jun 2016
SRR578565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8160745
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1184701.451705695987315No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167860.20569200483534283No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85890.10524774392534014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3200.032.654843
CGCGTAG1900.026.2500754-55
CAACGCA57800.020.2151412-13
ATCAACG58550.020.11843910-11
ACGTACG2950.019.32096120-21
CGTACGC2850.017.49902720-21
TCAACGC56250.017.3945910-11
CGTAGCG3050.017.13040556-57
CTCGCGT2850.016.66747752-53
GGTATCA72850.016.6256058
AACGCAG60050.016.53113712-13
AGTGGTA73600.016.520825
TCGCGTA3200.015.58669752-53
GTATCAA79300.015.5129159
ACGCAGA75950.015.259191514-15
ACTCGCG3200.014.84501850-51
AGAGTAC81650.014.339375518-19
TGGTATC86250.014.3179587
CGCTAGT3400.013.97011290-91
AAGCAGT93700.013.8073791
Produced by FastQC (version 0.11.5)