##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578565_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8160745 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.485909558502318 31.0 31.0 34.0 30.0 34.0 2 31.800590754888187 31.0 31.0 34.0 30.0 34.0 3 31.86918951639832 31.0 31.0 34.0 30.0 34.0 4 35.54482292976928 37.0 35.0 37.0 33.0 37.0 5 35.368288924601856 37.0 35.0 37.0 33.0 37.0 6 35.36411859456459 37.0 35.0 37.0 32.0 37.0 7 35.3429897638022 37.0 35.0 37.0 32.0 37.0 8 35.329820255381094 37.0 35.0 37.0 32.0 37.0 9 36.89866746234566 39.0 37.0 39.0 33.0 39.0 10-11 36.86458025584674 39.0 37.0 39.0 32.5 39.0 12-13 36.84116143807949 39.0 37.0 39.0 32.0 39.0 14-15 38.02281427737296 40.0 37.0 41.0 33.0 41.0 16-17 37.971795344665225 40.0 37.0 41.0 33.0 41.0 18-19 37.902692646320894 40.0 37.0 41.0 33.0 41.0 20-21 37.847418281051546 40.0 37.0 41.0 32.0 41.0 22-23 37.754412679234555 40.0 37.0 41.0 32.0 41.0 24-25 37.668864484798874 39.5 36.5 41.0 32.0 41.0 26-27 37.48235430711289 39.0 36.0 41.0 32.0 41.0 28-29 37.31265356287937 39.0 36.0 41.0 31.5 41.0 30-31 37.18195759088171 39.0 36.0 41.0 31.0 41.0 32-33 37.054844870168104 39.0 36.0 40.5 31.0 41.0 34-35 36.89912863347648 39.0 35.5 40.0 30.0 41.0 36-37 36.75018598179456 39.0 35.0 40.0 30.0 41.0 38-39 36.58663712687996 38.0 35.0 40.0 30.0 41.0 40-41 36.419920423931885 38.0 35.0 40.0 30.0 41.0 42-43 36.35746479028569 38.0 35.0 40.0 30.0 41.0 44-45 36.39109505320899 38.0 35.0 40.0 30.0 41.0 46-47 36.26223108306901 38.0 35.0 40.0 30.0 41.0 48-49 36.11717110386368 38.0 35.0 40.0 29.0 41.0 50-51 35.91349956407166 38.0 34.5 40.0 28.5 41.0 52-53 35.97446961031132 38.0 35.0 40.0 28.5 41.0 54-55 35.96056947006676 38.0 35.0 40.0 28.5 41.0 56-57 35.792130007738265 38.0 34.0 40.0 28.0 41.0 58-59 35.576279432821394 38.0 34.0 40.0 28.0 41.0 60-61 35.30406231293834 38.0 34.0 40.0 28.0 41.0 62-63 35.009746230276775 37.0 34.0 40.0 27.5 41.0 64-65 34.67416988277418 37.0 33.0 39.5 26.5 41.0 66-67 34.31597611492578 36.0 33.0 39.0 26.0 41.0 68-69 33.94018278968403 36.0 32.5 39.0 26.0 40.5 70-71 33.543528623918526 35.0 32.0 38.5 26.0 40.0 72-73 33.14330450222376 35.0 32.0 38.0 26.0 40.0 74-75 32.72982815907126 35.0 31.5 37.0 25.0 39.0 76-77 31.379107238371006 34.0 29.5 36.0 23.0 38.5 78-79 32.02266153396535 34.5 31.0 36.0 25.0 39.0 80-81 31.903588275335157 35.0 31.0 36.0 24.5 37.5 82-83 31.604255677637276 34.0 31.0 35.5 24.0 37.0 84-85 31.28623691832057 34.0 31.0 35.0 23.0 37.0 86-87 30.947099805226117 34.0 31.0 35.0 22.0 36.0 88-89 30.632046338906555 34.0 31.0 35.0 20.0 36.0 90-91 30.316110906541006 34.0 30.0 35.0 19.5 36.0 92-93 29.957405727050656 34.0 30.0 35.0 18.0 35.0 94-95 29.58817203576389 34.0 30.0 35.0 13.0 35.0 96-97 29.19435094467478 34.0 29.0 35.0 4.5 35.0 98-99 28.713109955034742 34.0 29.0 35.0 2.0 35.0 100-101 27.588660226486674 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4102810571887616 1101 2 0.41213960214619405 1101 3 0.3753003762875906 1101 4 0.2671913093996139 1101 5 0.3147926083668011 1101 6 0.25907108386854105 1101 7 0.2694943603556226 1101 8 0.25885907313013945 1101 9 0.3353721814105697 1101 10-11 0.33927174046284136 1101 12-13 0.3350852299127638 1101 14-15 0.4550718885259144 1101 16-17 0.3854423269734184 1101 18-19 0.37989516289550807 1101 20-21 0.4247861126071939 1101 22-23 0.4690949509484952 1101 24-25 0.46352049341847845 1101 26-27 0.42182006635075453 1101 28-29 0.5530847983036011 1101 30-31 0.5132324374237456 1101 32-33 0.46879824573081663 1101 34-35 0.5038573570511602 1101 36-37 0.44911499833658297 1101 38-39 0.4653751540661162 1101 40-41 0.4504151600857327 1101 42-43 0.5030936919241071 1101 44-45 0.429906415103801 1101 46-47 0.3971802677204579 1101 48-49 0.41478937334891697 1101 50-51 0.46074000421830874 1101 52-53 0.4223368752929346 1101 54-55 0.4797716701880148 1101 56-57 0.5344688802330566 1101 58-59 0.4531348728745499 1101 60-61 0.42979167752164216 1101 62-63 0.43999490895678406 1101 64-65 0.4028468749940117 1101 66-67 0.43445921623523986 1101 68-69 0.5223312227249153 1101 70-71 0.4957853629002358 1101 72-73 0.4259711765914389 1101 74-75 0.36332049951910506 1101 76-77 0.2645735790871342 1101 78-79 0.4316854642171819 1101 80-81 0.41180669733731534 1101 82-83 0.4048023500333109 1101 84-85 0.4518338779955471 1101 86-87 0.4526218324822828 1101 88-89 0.32868376061498594 1101 90-91 0.4744635515990119 1101 92-93 0.4906105620708594 1101 94-95 0.4948988572379349 1101 96-97 0.4413280335475065 1101 98-99 0.5473765613619328 1101 100-101 0.6249288911626998 1102 1 0.27270048909888445 1102 2 0.23186998299049222 1102 3 0.27353715461380546 1102 4 0.21392021327538657 1102 5 0.27354170109906306 1102 6 0.13841221291685457 1102 7 0.15080631927562393 1102 8 0.06504075654844854 1102 9 0.3411381054299554 1102 10-11 0.2791506598142135 1102 12-13 0.1682915683883408 1102 14-15 0.3557615930055036 1102 16-17 0.3554074887973968 1102 18-19 0.41635160137415284 1102 20-21 0.4015128173202811 1102 22-23 0.3293418499481007 1102 24-25 0.29771579897539624 1102 26-27 0.29827038246907733 1102 28-29 0.3206879643738674 1102 30-31 0.29490841206911966 1102 32-33 0.34171067018550616 1102 34-35 0.3611610659435627 1102 36-37 0.28350562664550694 1102 38-39 0.3757133454674815 1102 40-41 0.2607533967373641 1102 42-43 0.28389545415027584 1102 44-45 0.28676649043643465 1102 46-47 0.28071123156911426 1102 48-49 0.28568019713896575 1102 50-51 0.25779323934047227 1102 52-53 0.33110359300189884 1102 54-55 0.2770386901687161 1102 56-57 0.3641220777070018 1102 58-59 0.4362868884116793 1102 60-61 0.4234501023057504 1102 62-63 0.30721756558151014 1102 64-65 0.27572821251933277 1102 66-67 0.3465546562759343 1102 68-69 0.33282414739409205 1102 70-71 0.4001885753067711 1102 72-73 0.49564723449729087 1102 74-75 0.35102799459135525 1102 76-77 0.3413667111835075 1102 78-79 0.38355968478143865 1102 80-81 0.4624833697417081 1102 82-83 0.41478734052170196 1102 84-85 0.29610508181539785 1102 86-87 0.3178869628120822 1102 88-89 0.4363838391694408 1102 90-91 0.5396461002023187 1102 92-93 0.44488791087616164 1102 94-95 0.2727448724094401 1102 96-97 0.19089992082729879 1102 98-99 0.3504016996919823 1102 100-101 0.569959869491445 1103 1 0.21891319755721028 1103 2 0.27601126359372685 1103 3 0.2996216629356212 1103 4 0.16330522840408435 1103 5 0.25861501141341847 1103 6 0.19910808867336272 1103 7 0.1664913905064438 1103 8 0.169730106524824 1103 9 0.31455090497311033 1103 10-11 0.3113234726425418 1103 12-13 0.31360914268825013 1103 14-15 0.4171316233808895 1103 16-17 0.36238794832748056 1103 18-19 0.32147201888957966 1103 20-21 0.3207072356604215 1103 22-23 0.35071210597082114 1103 24-25 0.3458331184855439 1103 26-27 0.3143798919264995 1103 28-29 0.39195435625222075 1103 30-31 0.3180948194861557 1103 32-33 0.37501104130902263 1103 34-35 0.33254826026515616 1103 36-37 0.42723641343796714 1103 38-39 0.45622468594248033 1103 40-41 0.4099829216744908 1103 42-43 0.3921553267376936 1103 44-45 0.29489208073282924 1103 46-47 0.33884073751057997 1103 48-49 0.2679847998138172 1103 50-51 0.3822078909616238 1103 52-53 0.3403438295511094 1103 54-55 0.3029589478727317 1103 56-57 0.3881977421278222 1103 58-59 0.43860307726641423 1103 60-61 0.47228703773757275 1103 62-63 0.32179599145384685 1103 64-65 0.38700916381312567 1103 66-67 0.3462636891897617 1103 68-69 0.380423901217263 1103 70-71 0.26520845881964306 1103 72-73 0.30142750088504755 1103 74-75 0.37835840070347615 1103 76-77 0.32960774148042304 1103 78-79 0.39328179639221617 1103 80-81 0.42425045095057 1103 82-83 0.37381978978939756 1103 84-85 0.42688704424526236 1103 86-87 0.47951931928414737 1103 88-89 0.4613826371917682 1103 90-91 0.46397887723383846 1103 92-93 0.3365556467867705 1103 94-95 0.4020967247528979 1103 96-97 0.5204345770144876 1103 98-99 0.6913133990199967 1103 100-101 0.7858037640052977 1104 1 0.1406850932617516 1104 2 0.161830357307025 1104 3 0.2219231822910004 1104 4 0.20137943702879824 1104 5 0.20428163620441353 1104 6 0.20169745415882545 1104 7 0.12474751175108878 1104 8 0.14162202103903354 1104 9 0.2851869443019055 1104 10-11 0.21838175077961353 1104 12-13 0.22374773410416537 1104 14-15 0.2376019440502759 1104 16-17 0.23568969515777383 1104 18-19 0.2581190327673042 1104 20-21 0.28306010039932517 1104 22-23 0.32795513193303094 1104 24-25 0.30561336557057217 1104 26-27 0.2978063943712499 1104 28-29 0.2797823337060805 1104 30-31 0.2818832053295708 1104 32-33 0.2640339827652838 1104 34-35 0.31391380630994803 1104 36-37 0.37176109073490693 1104 38-39 0.3759438199047196 1104 40-41 0.3648841354423027 1104 42-43 0.27171193137201755 1104 44-45 0.3030259735369114 1104 46-47 0.34701076729157165 1104 48-49 0.17795166925191808 1104 50-51 0.28304089986212233 1104 52-53 0.34428785243000704 1104 54-55 0.36496465179346416 1104 56-57 0.4399197652922169 1104 58-59 0.42347620018679066 1104 60-61 0.421436435931426 1104 62-63 0.4066370015595524 1104 64-65 0.4584141912795019 1104 66-67 0.4541454060378527 1104 68-69 0.4747316725111048 1104 70-71 0.31763719072344543 1104 72-73 0.3855013300678465 1104 74-75 0.3454036946981631 1104 76-77 0.3158676272922598 1104 78-79 0.34173700464955914 1104 80-81 0.41426710439725056 1104 82-83 0.27237385707968187 1104 84-85 0.23898161264325068 1104 86-87 0.35766207475681 1104 88-89 0.35284235521798735 1104 90-91 0.49549125032548247 1104 92-93 0.5047640480522304 1104 94-95 0.48657160194461113 1104 96-97 0.525380850424412 1104 98-99 0.6298225756829865 1104 100-101 0.8130955664721959 1105 1 0.18553975128597244 1105 2 0.16897845905548792 1105 3 0.1259596403406391 1105 4 0.12263738499255794 1105 5 0.11419299666340521 1105 6 0.18594888397526432 1105 7 0.21541018400992584 1105 8 0.1627337736762513 1105 9 0.158149523963111 1105 10-11 0.14211289081992362 1105 12-13 0.17463478314459735 1105 14-15 0.14055649858301678 1105 16-17 0.1043703785646386 1105 18-19 0.23415770589765117 1105 20-21 0.1594328875881743 1105 22-23 0.17968155130953534 1105 24-25 0.18181900494817427 1105 26-27 0.22350797579363046 1105 28-29 0.24042358141383602 1105 30-31 0.1915300633646808 1105 32-33 0.18917707334756528 1105 34-35 0.22752162808673404 1105 36-37 0.321221700426797 1105 38-39 0.27560574775119306 1105 40-41 0.27551492506410824 1105 42-43 0.22704257755826518 1105 44-45 0.2245789629880406 1105 46-47 0.24034649726959856 1105 48-49 0.24740483482012365 1105 50-51 0.2269107184797008 1105 52-53 0.18358251263299508 1105 54-55 0.27146337176731805 1105 56-57 0.2789501409481119 1105 58-59 0.27721398546920994 1105 60-61 0.19691233331145241 1105 62-63 0.2438662260997333 1105 64-65 0.2351353271438299 1105 66-67 0.17342114727285463 1105 68-69 0.13266275081965517 1105 70-71 0.07268132892995993 1105 72-73 0.1639456534696322 1105 74-75 0.2028145558561789 1105 76-77 0.2164365399055086 1105 78-79 0.163891261417362 1105 80-81 0.15721239916638652 1105 82-83 0.20062550477291197 1105 84-85 0.2818176422018688 1105 86-87 0.26159754691397197 1105 88-89 0.2935812480407627 1105 90-91 0.22992892848514757 1105 92-93 0.18739142427098088 1105 94-95 0.28397280389824076 1105 96-97 0.3136741852102567 1105 98-99 0.4634590119449449 1105 100-101 0.4896726494650956 1106 1 -0.06981927139256427 1106 2 -0.006556360187694565 1106 3 -0.0963752742087145 1106 4 0.020677023695533592 1106 5 0.0044663954114270155 1106 6 0.06756700947616423 1106 7 0.0642050374766896 1106 8 0.10405956915786874 1106 9 -0.009458507747851286 1106 10-11 -0.027302275857572056 1106 12-13 -0.061844217007028135 1106 14-15 0.19261883748310282 1106 16-17 0.09840408229240438 1106 18-19 -0.00217608230604327 1106 20-21 0.08637174001523107 1106 22-23 0.06032235534705421 1106 24-25 0.16124504519336114 1106 26-27 0.19008613768415472 1106 28-29 0.16600466747097897 1106 30-31 0.12882683378511217 1106 32-33 0.1086308823453308 1106 34-35 0.06919017921632076 1106 36-37 0.07563108375254757 1106 38-39 0.01591744225159175 1106 40-41 -0.041661889543725295 1106 42-43 -0.03144435033394899 1106 44-45 0.03209398454023216 1106 46-47 0.0828716342722231 1106 48-49 0.10793807284728274 1106 50-51 0.027410469384022917 1106 52-53 -0.12087569344494398 1106 54-55 -0.13700869702640972 1106 56-57 -0.15492245202955957 1106 58-59 0.0022310755985230912 1106 60-61 0.09376926575237832 1106 62-63 0.01916234670536454 1106 64-65 -0.052567360923397644 1106 66-67 -0.05278588372330972 1106 68-69 0.0196909311215947 1106 70-71 -0.11280258815367006 1106 72-73 -0.01512896693022725 1106 74-75 -0.056931106630017325 1106 76-77 -0.1474871285156567 1106 78-79 -0.06253215790813016 1106 80-81 0.092653125226132 1106 82-83 0.08687139469428473 1106 84-85 0.1844790670291232 1106 86-87 0.3309526273827039 1106 88-89 0.18888100760928594 1106 90-91 0.2360165235205649 1106 92-93 0.14072003561674506 1106 94-95 0.3594978325983931 1106 96-97 0.40150592649732175 1106 98-99 0.44355791375793174 1106 100-101 0.4172799013982953 1107 1 0.04171126469091746 1107 2 0.08903714388650741 1107 3 0.05266577954245477 1107 4 0.12887858663433605 1107 5 -0.025718277461123762 1107 6 0.013358891834840847 1107 7 0.05556003991770808 1107 8 0.007683909035606007 1107 9 -0.07185309589542044 1107 10-11 -0.03611608481539719 1107 12-13 -0.11520872397268533 1107 14-15 -0.0335963264977579 1107 16-17 0.1522458479559532 1107 18-19 -0.028646066698627237 1107 20-21 -0.019494018358905407 1107 22-23 -0.11370138467858482 1107 24-25 -0.07219322105829207 1107 26-27 -0.07748564921728729 1107 28-29 -0.048633618830685066 1107 30-31 0.0057890800444511115 1107 32-33 0.02830570840126967 1107 34-35 0.06704009808935041 1107 36-37 0.06393884303443542 1107 38-39 0.06023067133865112 1107 40-41 0.09065107037893227 1107 42-43 0.10052105105403086 1107 44-45 0.037856231348875724 1107 46-47 -0.0012058562366377146 1107 48-49 -0.021179218974303637 1107 50-51 0.04704747509484264 1107 52-53 -0.05667039104354643 1107 54-55 0.002883335089293837 1107 56-57 0.044800459697569295 1107 58-59 0.0685590208373057 1107 60-61 0.20869488505366718 1107 62-63 0.18073794036049406 1107 64-65 0.16856035552563498 1107 66-67 0.02542621233267539 1107 68-69 0.12128121208940712 1107 70-71 0.12778411471285267 1107 72-73 0.006915350439321344 1107 74-75 0.05395995800445519 1107 76-77 -0.009800644619861743 1107 78-79 0.019916936030931254 1107 80-81 0.16052093117233213 1107 82-83 0.15129985635792664 1107 84-85 0.18998286794634112 1107 86-87 0.1730094760031342 1107 88-89 0.22937305661643137 1107 90-91 0.26850617064740234 1107 92-93 0.29485592518792814 1107 94-95 0.1646122240362189 1107 96-97 0.18945656229522712 1107 98-99 0.14248424991543018 1107 100-101 0.37780224513359784 1108 1 0.024438556193196348 1108 2 0.05219737095919186 1108 3 -0.049680128982672045 1108 4 -0.01271466583076375 1108 5 -0.09967977266335026 1108 6 -0.08198638039635142 1108 7 -0.01950325018413679 1108 8 -0.05950077060629866 1108 9 -0.13720295429461515 1108 10-11 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-0.9651307638681033 2316 96-97 -1.0602569346228456 2316 98-99 -1.152650107251688 2316 100-101 -1.6066166613796433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 130.0 3 62.0 4 212.0 5 575.0 6 1086.0 7 1985.0 8 3497.0 9 5222.0 10 7302.0 11 9569.0 12 11848.0 13 14336.0 14 16470.0 15 18884.0 16 21757.0 17 25648.0 18 29943.0 19 34002.0 20 36942.0 21 38913.0 22 44366.0 23 52395.0 24 64181.0 25 81856.0 26 103755.0 27 131334.0 28 165304.0 29 204199.0 30 251152.0 31 304558.0 32 370131.0 33 458931.0 34 591966.0 35 824817.0 36 1071833.0 37 1509248.0 38 1369487.0 39 282419.0 40 430.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.260212871519805 31.363114762447108 27.716837412452467 22.659834953580617 2 23.034673917638646 28.939183861277375 27.954249275035554 20.07189294604843 3 21.082033564337568 28.19999889715951 28.497606529795995 22.220361008706927 4 21.688638109570753 28.739086016241373 28.15157422011375 21.420701654074122 5 22.468765010930408 28.513415482947586 27.99572831808958 21.02209118803243 6 22.139153052280395 28.388873510247752 27.84057287460133 21.63140056287052 7 22.375334604867568 28.711177717230473 27.69537585110183 21.218111826800126 8 21.507362514461022 28.52533676211114 28.0987450756556 21.86855564777224 9 21.34217944063072 28.312134445864935 28.55388986790025 21.791796245604097 10-11 21.484195333547326 28.83958970134872 27.911058539616235 21.765156425487717 12-13 21.48191390392837 28.903541147718887 27.749424562270303 21.865120386082445 14-15 21.63243326833926 28.891556946278428 27.581443554656293 21.894566230726024 16-17 21.83215024070414 28.72258162230174 27.508470581329924 21.936797555664192 18-19 21.83333941938123 28.733208328360337 27.486232256821747 21.94721999543669 20-21 21.919753718397704 28.667126306384365 27.514816518772594 21.89830345644534 22-23 21.8597936080133 28.704503093992717 27.50988008614667 21.925823211847316 24-25 21.839654345283428 28.656194992001343 27.457536046035013 22.04661461668022 26-27 22.015042744259254 28.527573156617443 27.587867284175648 21.869516814947655 28-29 22.027983853979467 28.608992072205393 27.487657588094127 21.875366485721013 30-31 21.97115820868344 28.640624089568647 27.419026254550282 21.96919144719763 32-33 22.002867884909147 28.681967689607703 27.341497402556982 21.97366702292617 34-35 22.02597392730692 28.696210948001905 27.3465877161359 21.931227408555273 36-37 22.00880155020607 28.695140497074924 27.34134475732323 21.954713195395776 38-39 22.028514264024842 28.676971602454582 27.32072588558329 21.973788247937282 40-41 22.017647267865698 28.766345565880396 27.30929713607182 21.906710030182087 42-43 22.039890435909747 28.770487052402228 27.25491909214194 21.934703419546086 44-45 22.022997269354573 28.778232231811256 27.267971112310352 21.930799386523816 46-47 22.04802500910667 28.82058741622093 27.19040002254839 21.940987552124007 48-49 22.053661456170907 28.846200507928977 27.175603380114886 21.92453465578523 50-51 22.066374548784243 28.808220557987962 27.18946258021886 21.935942313008937 52-53 22.022067870521695 28.90003906858915 27.137099620490567 21.940793440398586 54-55 22.062145277777116 28.90260848604897 27.058869375215867 21.976376860958048 56-57 22.070919995662162 28.90131354429038 27.073894601534544 21.95387185851292 58-59 22.042582397215547 29.017848007221893 27.02163883852242 21.91793075704014 60-61 22.029938220773975 28.997789642813732 27.024673948419935 21.947598187992362 62-63 22.04295422677037 29.039050266233712 27.01400016934849 21.90399533764743 64-65 22.025166198815104 29.13018698867794 26.97002514977867 21.874621662728284 66-67 22.00207077938394 29.130170339035445 26.978797334124277 21.88896154745634 68-69 21.999781880520768 29.18740078167649 26.960725627139453 21.852091710663284 70-71 22.012678420486242 29.2543303599547 26.90398103677678 21.829010182782277 72-73 21.971723028934097 29.226486890706514 26.889824217858465 21.911965862500924 74-75 22.024682868052487 29.258191152073653 26.8756508724977 21.84147510737616 76-77 21.992894031120933 29.345237475255015 26.834835545039088 21.827032948584964 78-79 22.010018693146275 29.342419105118466 26.8164671240187 21.831095077716554 80-81 21.931456520600324 29.378508608142713 26.826200956838125 21.863833914418834 82-83 21.9452018486333 29.491216565573808 26.760493222942085 21.803088362850804 84-85 21.938627089180947 29.46826229111254 26.787510780280304 21.80559983942621 86-87 21.890027927701556 29.542178941565783 26.74690282273755 21.820890307995107 88-89 21.888361397322463 29.611730017121214 26.741690231006505 21.758218354549815 90-91 21.876822567160996 29.61114584734228 26.696269900618297 21.815761684878428 92-93 21.889000780079567 29.638998145503585 26.686404338056047 21.785596736360798 94-95 21.873084191009195 29.7147113421529 26.653662907315617 21.758541559522282 96-97 21.862747050254804 29.762981865749254 26.623972012076223 21.750299071919713 98-99 21.808345929201316 29.80827118112378 26.618721697590107 21.76466119208479 100-101 21.9005522045539 29.783289366753817 26.51789469195456 21.798263736737727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 128964.0 1 73494.5 2 13760.5 3 8829.0 4 7589.5 5 6695.5 6 6088.5 7 5830.5 8 5665.0 9 5271.5 10 5057.0 11 5493.0 12 8778.0 13 8741.5 14 5970.5 15 6219.5 16 6609.5 17 7515.0 18 9232.0 19 10855.0 20 13018.0 21 16513.0 22 20527.5 23 25903.0 24 32324.0 25 39566.5 26 50455.0 27 62563.0 28 71718.5 29 81307.5 30 95566.0 31 112831.5 32 130570.5 33 148516.5 34 166361.0 35 182642.0 36 198462.0 37 216138.5 38 230096.5 39 235635.0 40 245068.0 41 256355.0 42 261778.5 43 272575.0 44 285613.5 45 294562.5 46 298438.5 47 297735.5 48 300209.5 49 303248.0 50 302474.0 51 298711.0 52 294962.5 53 285766.5 54 267035.5 55 247645.0 56 225766.5 57 203367.5 58 187264.0 59 164424.5 60 133584.0 61 104510.0 62 76690.5 63 54258.0 64 37562.5 65 24035.5 66 14662.5 67 9155.5 68 5143.5 69 2558.5 70 1270.5 71 567.5 72 287.0 73 177.0 74 118.5 75 76.5 76 47.5 77 32.0 78 26.0 79 21.0 80 15.0 81 9.5 82 7.0 83 6.0 84 5.0 85 5.0 86 4.0 87 4.0 88 3.0 89 3.0 90 2.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14564846714362475 2 0.0 3 0.0 4 0.0027938625701452503 5 3.063445800597862E-4 6 9.312875233817501E-4 7 0.0 8 1.7155296483348028E-4 9 2.2056809764304608E-4 10-11 7.96495908155444E-5 12-13 1.2253783202391448E-5 14-15 1.2253783202391448E-5 16-17 6.126891601195724E-6 18-19 3.676134960717434E-5 20-21 6.739580761315298E-5 22-23 1.4091850682750165E-4 24-25 0.0 26-27 0.0 28-29 0.005734770538719198 30-31 0.001488834659090561 32-33 3.308521464645691E-4 34-35 2.818370136550033E-4 36-37 2.87963905256199E-4 38-39 0.001133474946221209 40-41 0.0033697903806576483 42-43 5.146588945004408E-4 44-45 4.533899784884836E-4 46-47 0.006341332807237574 48-49 0.006727326978112905 50-51 0.006972402642160734 52-53 0.009031038220162498 54-55 0.0048831326061529925 56-57 0.0 58-59 2.4507566404782896E-5 60-61 8.945261737745757E-4 62-63 7.10719425738704E-4 64-65 4.533899784884836E-4 66-67 6.065622685183767E-4 68-69 9.803026561913159E-4 70-71 0.001311154802655885 72-73 0.00266519784652014 74-75 7.903690165542484E-4 76-77 0.0 78-79 0.0 80-81 1.531722900298931E-4 82-83 1.6542607323228456E-4 84-85 1.3479161522630595E-4 86-87 0.0025671675809010083 88-89 0.001335662369060668 90-91 4.5951687008967927E-4 92-93 4.778975448932665E-4 94-95 3.4310592966696056E-4 96-97 0.0010109371141972946 98-99 0.0 100-101 1.2253783202391448E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8160745.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.11657521367886 #Duplication Level Percentage of deduplicated Percentage of total 1 91.34143820003072 58.56500192477869 2 4.6312705010355435 5.938824068290752 3 1.2646613036732801 2.4325725479039106 4 0.6481076533287157 1.66217772404846 5 0.37773772758339075 1.2109624710822304 6 0.22780866254187723 0.8763786747716313 7 0.1640010264929802 0.7360628905180393 8 0.11813808326719157 0.6059687441120595 9 0.09368614999687738 0.540615157447931 >10 1.0139344472795258 14.921457150204546 >50 0.10455908201432078 4.333671379012943 >100 0.014169265175766629 1.1690224783283 >500 0.0 0.0 >1k 2.0909896280669842E-4 0.3444630528980705 >5k 1.3939930853779897E-4 0.618362125447665 >10k+ 1.3939930853779897E-4 6.04445961115483 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118470 1.451705695987315 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16786 0.20569200483534283 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8589 0.10524774392534014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2253783202391448E-5 0.0 2 1.2253783202391448E-5 0.0 0.0 2.4507566404782896E-5 2.4507566404782896E-5 3 2.4507566404782896E-5 0.0 0.0 2.4507566404782896E-5 2.4507566404782896E-5 4 3.676134960717434E-5 0.0 0.0 2.4507566404782896E-5 2.4507566404782896E-5 5 6.126891601195724E-5 0.0 0.0 2.4507566404782896E-5 2.4507566404782896E-5 6 8.577648241674014E-5 0.0 0.0 2.4507566404782896E-5 2.4507566404782896E-5 7 1.1028404882152304E-4 0.0 0.0 2.4507566404782896E-5 3.676134960717434E-5 8 1.3479161522630593E-4 0.0 0.0 2.4507566404782896E-5 4.901513280956579E-5 9 1.5929918163108884E-4 0.0 0.0 2.4507566404782896E-5 4.901513280956579E-5 10-11 2.1444120604185036E-4 0.0 0.0 2.4507566404782896E-5 7.352269921434868E-5 12-13 2.4507566404782897E-4 0.0 0.0 3.063445800597862E-5 9.803026561913158E-5 14-15 2.9409079685739474E-4 0.0 0.0 4.901513280956579E-5 1.2253783202391448E-4 16-17 3.6148660447054773E-4 0.0 0.0 6.126891601195724E-5 1.531722900298931E-4 18-19 4.288824120837007E-4 0.0 0.0 6.126891601195724E-5 1.7155296483348028E-4 20-21 4.778975448932665E-4 0.0 0.0 6.739580761315296E-5 2.021874228394589E-4 22-23 5.269126777028323E-4 0.0 0.0 7.352269921434868E-5 2.2056809764304608E-4 24-25 5.943084853159853E-4 0.0 0.0 7.352269921434868E-5 2.266949892442418E-4 26-27 6.433236181255511E-4 0.0 0.0 8.577648241674013E-5 2.4507566404782897E-4 28-29 6.739580761315297E-4 0.0 0.0 1.1028404882152304E-4 2.573294472502204E-4 30-31 7.291001005422912E-4 0.0 0.0 1.2253783202391448E-4 2.87963905256199E-4 32-33 8.026227997566398E-4 0.0 0.0 1.2253783202391448E-4 3.492328212681563E-4 34-35 8.8839928217338E-4 0.0 0.0 1.2253783202391448E-4 3.6761349607174345E-4 36-37 9.803026561913159E-4 0.0 0.0 1.2253783202391448E-4 3.9824795407772206E-4 38-39 0.0010599522470068601 0.0 0.0 1.3479161522630593E-4 4.166286288813092E-4 40-41 0.001219251428637949 0.0 0.0 1.3479161522630593E-4 4.288824120837007E-4 42-43 0.0013662968270666464 0.0 0.0 1.3479161522630593E-4 4.533899784884836E-4 44-45 0.001439819526280995 0.0 0.0 1.3479161522630593E-4 5.146588945004408E-4 46-47 0.0015010884422929525 0.0 0.0 1.3479161522630593E-4 5.146588945004408E-4 48-49 0.0015807380331084968 0.0 0.0 1.3479161522630593E-4 5.146588945004408E-4 50-51 0.0016481338407216498 0.0 0.0 1.3479161522630593E-4 5.269126777028323E-4 52-53 0.001727783431537194 0.0 0.0 1.3479161522630593E-4 5.391664609052237E-4 54-55 0.00181968680555513 0.0 0.0 1.4704539842869737E-4 5.636740273100066E-4 56-57 0.0018625750467635002 0.0 0.0 1.4704539842869737E-4 5.636740273100066E-4 58-59 0.001978985987186219 0.0 0.0 1.4704539842869737E-4 5.881815937147895E-4 60-61 0.0020892700360077418 0.0 0.0 1.4704539842869737E-4 6.004353769171809E-4 62-63 0.002199554084829265 0.0 0.0 1.5929918163108884E-4 6.065622685183767E-4 64-65 0.0023404725916567667 0.0 0.0 1.6542607323228456E-4 6.249429433219639E-4 66-67 0.0025058986648890513 0.0 0.0 1.8380674803587172E-4 6.617042929291382E-4 68-69 0.002689705412924923 0.0 0.0 1.8380674803587172E-4 7.413538837446826E-4 70-71 0.0028735121609607945 0.0 0.0 1.8380674803587172E-4 7.78115233351857E-4 72-73 0.003106334041806232 0.0 0.0 1.9606053123826317E-4 8.087496913578356E-4 74-75 0.003247252548633734 0.0 0.0 1.9606053123826317E-4 8.577648241674014E-4 76-77 0.0035229626706875412 0.0 0.0 2.021874228394589E-4 8.700186073697929E-4 78-79 0.003835434142348523 0.0 0.0 2.083143144406546E-4 9.006530653757715E-4 80-81 0.004264316554432225 0.0 0.0 2.1444120604185036E-4 9.619219813877287E-4 82-83 0.004748340990926686 0.0 0.0 2.2056809764304608E-4 9.741757645901201E-4 84-85 0.005361030151046258 0.0 0.0 2.2056809764304608E-4 9.986833309949031E-4 86-87 0.006310698349231596 0.0 0.0 2.2056809764304608E-4 0.0010354446806020772 88-89 0.0076953758511018305 0.0 0.0 2.266949892442418E-4 0.0010722060302092518 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 320 0.0 32.65484 3 CGCGTAG 190 0.0 26.25007 54-55 CAACGCA 5780 0.0 20.21514 12-13 ATCAACG 5855 0.0 20.118439 10-11 ACGTACG 295 0.0 19.320961 20-21 CGTACGC 285 0.0 17.499027 20-21 TCAACGC 5625 0.0 17.39459 10-11 CGTAGCG 305 0.0 17.130405 56-57 CTCGCGT 285 0.0 16.667477 52-53 GGTATCA 7285 0.0 16.625605 8 AACGCAG 6005 0.0 16.531137 12-13 AGTGGTA 7360 0.0 16.52082 5 TCGCGTA 320 0.0 15.586697 52-53 GTATCAA 7930 0.0 15.512915 9 ACGCAGA 7595 0.0 15.2591915 14-15 ACTCGCG 320 0.0 14.845018 50-51 AGAGTAC 8165 0.0 14.3393755 18-19 TGGTATC 8625 0.0 14.317958 7 CGCTAGT 340 0.0 13.970112 90-91 AAGCAGT 9370 0.0 13.807379 1 >>END_MODULE