FastQCFastQC Report
Sat 18 Jun 2016
SRR578564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174530
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116834914.292552599354336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC290040.35480938965298314No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT255960.31311891937518116No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG95440.1167528897685861No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT91320.11171284465284242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2507600.092.07385
GGTATCA2522150.091.891668
GTATCAA2529400.091.727679
TGGTATC2522100.091.6698157
AAGCAGT2532700.091.649371
GTGGTAT2537950.091.225566
GCAGTGG2547250.090.8766253
CAGTGGT2546200.090.70994
AGCAGTG2562150.090.503192
TCAACGC2518000.046.53130312-13
AACGCAG2528400.046.41868214-15
CAACGCA2518100.046.37475212-13
ATCAACG2517200.046.35547310-11
CGCAGAG2536350.046.2471516-17
ACGCAGA2530250.046.2242114-15
TATCAAC2535150.046.1247110-11
CAGAGTA2517600.046.01550318-19
AGAGTAC2473750.045.94405718-19
GAGTACT1445200.045.791820-21
GCAGAGT2550050.045.6391716-17
Produced by FastQC (version 0.11.5)