FastQCFastQC Report
Sat 18 Jun 2016
SRR578564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174530
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1214911.4862138863029435No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163890.20048859078136602No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86120.10535162266209801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.029.1191043
CAACGCA58350.020.58510812-13
ATCAACG61150.019.95308910-11
CGTACGC2950.019.3123620-21
CGCGTAG2850.019.15847654-55
CGTAGCG3050.018.67968256-57
ACGTACG3300.017.98341820-21
CTCGCGT3300.017.2663552-53
TCAACGC57750.017.0584510-11
GGTATCA74050.017.054158
AGTGGTA75450.016.7379075
TCGCGTA3450.016.5156452-53
ACTCGCG3500.016.2803550-51
AACGCAG61500.016.13409612-13
GTATCAA79800.016.0632849
CGCTAGT3750.015.19331890-91
ACGCAGA79800.015.14113314-15
TGGTATC86450.014.6079787
AAGCAGT94450.014.3985281
AGAGTAC85300.013.97014118-19
Produced by FastQC (version 0.11.5)