##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578564_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8174530 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39843526172147 31.0 31.0 34.0 30.0 34.0 2 31.77223253202325 31.0 31.0 34.0 30.0 34.0 3 31.843400782674966 31.0 31.0 34.0 30.0 34.0 4 35.55307179739997 37.0 35.0 37.0 33.0 37.0 5 35.3770159262979 37.0 35.0 37.0 33.0 37.0 6 35.3687624854273 37.0 35.0 37.0 32.0 37.0 7 35.36141429537845 37.0 35.0 37.0 32.0 37.0 8 35.31854504173329 37.0 35.0 37.0 32.0 37.0 9 36.86076239245559 39.0 37.0 39.0 32.0 39.0 10-11 36.85622928780003 39.0 37.0 39.0 32.0 39.0 12-13 36.84657062852543 39.0 37.0 39.0 32.0 39.0 14-15 38.01653244896037 40.0 37.0 41.0 33.0 41.0 16-17 37.945363158493514 40.0 37.0 41.0 33.0 41.0 18-19 37.86877300590982 40.0 37.0 41.0 32.5 41.0 20-21 37.82056772682955 40.0 37.0 41.0 32.0 41.0 22-23 37.613759690159554 39.5 36.0 41.0 32.0 41.0 24-25 37.61836839549185 39.0 36.0 41.0 32.0 41.0 26-27 37.445508671446554 39.0 36.0 41.0 32.0 41.0 28-29 37.293928091278644 39.0 36.0 41.0 31.5 41.0 30-31 37.15406286355301 39.0 36.0 41.0 31.0 41.0 32-33 37.014710998675156 39.0 36.0 40.5 31.0 41.0 34-35 36.86479149259958 39.0 35.5 40.0 30.0 41.0 36-37 36.71035778203762 39.0 35.0 40.0 30.0 41.0 38-39 36.5403584670923 38.0 35.0 40.0 30.0 41.0 40-41 36.37765492327999 38.0 35.0 40.0 30.0 41.0 42-43 36.461135624922775 38.0 35.0 40.0 30.0 41.0 44-45 36.38894817194383 38.0 35.0 40.0 30.0 41.0 46-47 36.23622624175335 38.0 35.0 40.0 30.0 41.0 48-49 36.0863529768684 38.0 35.0 40.0 29.0 41.0 50-51 35.88980724274056 38.0 34.5 40.0 28.5 41.0 52-53 35.9337245077087 38.0 35.0 40.0 28.0 41.0 54-55 35.91707945288598 38.0 34.5 40.0 28.0 41.0 56-57 35.75164339723507 38.0 34.0 40.0 28.0 41.0 58-59 35.5172640506549 38.0 34.0 40.0 28.0 41.0 60-61 35.24799566458255 38.0 34.0 40.0 28.0 41.0 62-63 34.95770710976656 37.0 34.0 40.0 27.0 41.0 64-65 34.6260181319293 37.0 33.0 39.5 26.5 41.0 66-67 34.2733554712014 36.0 33.0 39.0 26.0 41.0 68-69 33.88713803729389 36.0 32.5 39.0 26.0 40.5 70-71 33.48202385947572 35.0 32.0 38.5 26.0 40.0 72-73 33.08393075809863 35.0 32.0 38.0 26.0 40.0 74-75 32.66886487663511 35.0 31.5 37.0 25.0 39.0 76-77 31.297711244560848 33.5 29.5 36.0 23.0 38.5 78-79 31.950978833033826 34.5 31.0 36.0 24.0 39.0 80-81 31.828545494358696 34.5 31.0 36.0 24.0 37.5 82-83 31.46960112691494 34.0 31.0 35.5 23.0 37.0 84-85 31.19123943517242 34.0 31.0 35.0 23.0 37.0 86-87 30.878233427487572 34.0 31.0 35.0 21.5 36.0 88-89 30.574646799265523 34.0 30.5 35.0 20.0 36.0 90-91 30.23105634207716 34.0 30.0 35.0 19.5 36.0 92-93 29.857041566915775 34.0 30.0 35.0 17.5 35.0 94-95 29.471197732469022 34.0 29.5 35.0 9.5 35.0 96-97 28.998885318177315 34.0 29.0 35.0 2.0 35.0 98-99 28.17682184786159 34.0 29.0 35.0 2.0 35.0 100-101 27.118644068833316 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37079253095956943 1101 2 0.24940504004952757 1101 3 0.32670599574462855 1101 4 0.27392644981360803 1101 5 0.2561010754078481 1101 6 0.12725928641067696 1101 7 0.20129181349001612 1101 8 0.15827659886647893 1101 9 0.2578458577268634 1101 10-11 0.3001831686201015 1101 12-13 0.3420111833688324 1101 14-15 0.1820130885169462 1101 16-17 0.28536168819496766 1101 18-19 0.3047365513375908 1101 20-21 0.2220404609194162 1101 22-23 0.49055382805058656 1101 24-25 0.37142788925283554 1101 26-27 0.26910388099705784 1101 28-29 0.34892936930271645 1101 30-31 0.3821603245682397 1101 32-33 0.39877452527127843 1101 34-35 0.37279887628690744 1101 36-37 0.3959409321883953 1101 38-39 0.30848234908157224 1101 40-41 0.3020531210516282 1101 42-43 0.3317582763632885 1101 44-45 0.298295732549839 1101 46-47 0.2467137845076124 1101 48-49 0.3259782519235017 1101 50-51 0.33750318133596835 1101 52-53 0.3102961412336853 1101 54-55 0.4908940596087703 1101 56-57 0.5739905596005102 1101 58-59 0.506404326094092 1101 60-61 0.4640772280871843 1101 62-63 0.4194784827152347 1101 64-65 0.47059226933777865 1101 66-67 0.5503436292528434 1101 68-69 0.42809224752986097 1101 70-71 0.42590674602984535 1101 72-73 0.5361094615667739 1101 74-75 0.5223781801776965 1101 76-77 0.41719442323521605 1101 78-79 0.49766568772494324 1101 80-81 0.41181539424338 1101 82-83 0.5507762979646067 1101 84-85 0.4984822981762278 1101 86-87 0.4605190777151229 1101 88-89 0.5577046272226589 1101 90-91 0.4404614357552141 1101 92-93 0.5024091655026659 1101 94-95 0.36410897372667606 1101 96-97 0.4948840087576514 1101 98-99 0.7875639278039301 1101 100-101 0.975611219586046 1102 1 0.31922097065597654 1102 2 0.32430424363384347 1102 3 0.3389995254258089 1102 4 0.3244803269264409 1102 5 0.3395193192535828 1102 6 0.2210652756837348 1102 7 0.2998578830935088 1102 8 0.2283116131464169 1102 9 0.23485099776301865 1102 10-11 0.32857012316664935 1102 12-13 0.3334661308393265 1102 14-15 0.41482546365956097 1102 16-17 0.23794393668044478 1102 18-19 0.32475739439744444 1102 20-21 0.34255404851825944 1102 22-23 0.5147201460899637 1102 24-25 0.38703530251861906 1102 26-27 0.42181763894404867 1102 28-29 0.42940362191948367 1102 30-31 0.42169233047022914 1102 32-33 0.41528949264347403 1102 34-35 0.4080614183932383 1102 36-37 0.4496665014721941 1102 38-39 0.4204360210673883 1102 40-41 0.4632117161579288 1102 42-43 0.4245910444052541 1102 44-45 0.4081396852980248 1102 46-47 0.4299557020151994 1102 48-49 0.3813907400045835 1102 50-51 0.3550984140914011 1102 52-53 0.41881844762963283 1102 54-55 0.4578800661766209 1102 56-57 0.4689835783689986 1102 58-59 0.5529903439474069 1102 60-61 0.5553890122406528 1102 62-63 0.3706548257659179 1102 64-65 0.453103489206228 1102 66-67 0.4560620362825176 1102 68-69 0.4263560971083322 1102 70-71 0.49827512215628644 1102 72-73 0.5677489005619734 1102 74-75 0.4175559878823094 1102 76-77 0.24573215459165354 1102 78-79 0.46745350159583055 1102 80-81 0.5006920137878588 1102 82-83 0.5302022037635972 1102 84-85 0.5549130684640318 1102 86-87 0.6339843284398441 1102 88-89 0.49713834808337865 1102 90-91 0.44968732233776976 1102 92-93 0.47418481383860467 1102 94-95 0.6049055727057358 1102 96-97 0.760739923794663 1102 98-99 1.0939115247256126 1102 100-101 1.1091365944412495 1103 1 0.17452063326386735 1103 2 0.26405937346546793 1103 3 0.26009950494797707 1103 4 0.23062036115954498 1103 5 0.23509710570443332 1103 6 0.09160980637029326 1103 7 0.23094326064697412 1103 8 0.036897442187928675 1103 9 0.22360010730724156 1103 10-11 0.16101598037847253 1103 12-13 0.24589279834994926 1103 14-15 0.31987190311983227 1103 16-17 0.32471253883468165 1103 18-19 0.25101907379981725 1103 20-21 0.3763283304937275 1103 22-23 0.35420536886564946 1103 24-25 0.3570119346704317 1103 26-27 0.147811931927599 1103 28-29 0.24306141529495306 1103 30-31 0.2558582234713569 1103 32-33 0.210830123836665 1103 34-35 0.22235778758480507 1103 36-37 0.3332599626829733 1103 38-39 0.41748294839848654 1103 40-41 0.1966448791155102 1103 42-43 0.25765772920782837 1103 44-45 0.2705999245533306 1103 46-47 0.347825972907593 1103 48-49 0.28684737028112295 1103 50-51 0.2500088370037261 1103 52-53 0.22790359773487268 1103 54-55 0.37603415735009804 1103 56-57 0.31771062563688446 1103 58-59 0.24961508545779765 1103 60-61 0.2624790600490243 1103 62-63 0.3775791413232312 1103 64-65 0.24934672818082504 1103 66-67 0.22900532861996936 1103 68-69 0.3843436481009732 1103 70-71 0.29050205230605286 1103 72-73 0.19367350347767598 1103 74-75 0.2511424081710345 1103 76-77 0.20894122916200075 1103 78-79 0.3265142747333165 1103 80-81 0.2901281489931016 1103 82-83 0.5675618794698742 1103 84-85 0.41687428209906585 1103 86-87 0.38466187036582866 1103 88-89 0.41782221350822013 1103 90-91 0.3920245487367673 1103 92-93 0.33808892130831225 1103 94-95 0.4014368258173491 1103 96-97 0.4389072891707002 1103 98-99 0.9959908327318914 1103 100-101 0.8402955129158478 1104 1 0.2539997556572935 1104 2 0.197510141498892 1104 3 0.1369934961477206 1104 4 0.18279118682934836 1104 5 0.15525944688364035 1104 6 0.05667371962346124 1104 7 0.10502264426670394 1104 8 0.06948823576195906 1104 9 0.22928742548929648 1104 10-11 0.14432572744674133 1104 12-13 0.09483222352676535 1104 14-15 0.1974020648617909 1104 16-17 0.24900196367117644 1104 18-19 0.1013241189282752 1104 20-21 0.19870686643881896 1104 22-23 0.2776732519264442 1104 24-25 0.1502793148452355 1104 26-27 0.2516403342222375 1104 28-29 0.2578280589084443 1104 30-31 0.17004042229972782 1104 32-33 0.2068170721558218 1104 34-35 0.13886507354604305 1104 36-37 0.11038504021583151 1104 38-39 0.11812844895885632 1104 40-41 0.21179832203501547 1104 42-43 0.11878598616533509 1104 44-45 0.07439958226414234 1104 46-47 0.0996764347982051 1104 48-49 0.10745110686102777 1104 50-51 0.07360509946608573 1104 52-53 0.08146094850818741 1104 54-55 0.1875339432754899 1104 56-57 0.07947934017054337 1104 58-59 0.09085019059038046 1104 60-61 0.09352359991593318 1104 62-63 0.015783140109903115 1104 64-65 0.13074516717472306 1104 66-67 0.10633950456316654 1104 68-69 0.1385051296282782 1104 70-71 0.09608318078553424 1104 72-73 0.08665671028335709 1104 74-75 0.04853471214513405 1104 76-77 -0.032098553759766446 1104 78-79 0.1285595664723722 1104 80-81 0.21155672113215118 1104 82-83 0.22410285239524086 1104 84-85 0.10043309631826958 1104 86-87 0.23609546261166514 1104 88-89 0.22497587491441706 1104 90-91 0.14329192712117234 1104 92-93 0.2081035161224314 1104 94-95 0.28045041213409405 1104 96-97 0.22255427106684422 1104 98-99 0.611761189354425 1104 100-101 0.7477096972461119 1105 1 0.24707971055779865 1105 2 0.181012050045684 1105 3 0.12448022157740724 1105 4 0.1426496361463947 1105 5 0.18564134260125087 1105 6 0.11482681854470655 1105 7 0.2278350344088551 1105 8 0.08498803667335864 1105 9 0.2621245405098378 1105 10-11 0.13671914174305044 1105 12-13 0.15523730165484295 1105 14-15 0.20076467877175475 1105 16-17 0.15665033359327651 1105 18-19 0.248133557433583 1105 20-21 0.1819672903685543 1105 22-23 0.24642354531534494 1105 24-25 0.30604351128048535 1105 26-27 0.2549786523593056 1105 28-29 0.3369195326132015 1105 30-31 0.2455641600804057 1105 32-33 0.25392904272471384 1105 34-35 0.23369091831647637 1105 36-37 0.22965940336420942 1105 38-39 0.2738433631068915 1105 40-41 0.25894888954395867 1105 42-43 0.2465912316445582 1105 44-45 0.280781804801137 1105 46-47 0.263630329583286 1105 48-49 0.31381250847746855 1105 50-51 0.3097641211709643 1105 52-53 0.33149053056760636 1105 54-55 0.32833878758899715 1105 56-57 0.31389094109690063 1105 58-59 0.24509734966246555 1105 60-61 0.19051172618335244 1105 62-63 0.24092889571661402 1105 64-65 0.2291073261821026 1105 66-67 0.278250620913262 1105 68-69 0.21870336847442928 1105 70-71 0.30975153433677605 1105 72-73 0.3674747528331963 1105 74-75 0.3992204337273364 1105 76-77 0.28588074390166796 1105 78-79 0.39969594440413303 1105 80-81 0.48140949001713906 1105 82-83 0.46958637605509423 1105 84-85 0.45484401467931335 1105 86-87 0.46096236307974436 1105 88-89 0.47837545053979014 1105 90-91 0.6260193242075331 1105 92-93 0.7818478123249903 1105 94-95 0.7963322846907204 1105 96-97 0.8211597049803245 1105 98-99 1.29279415962748 1105 100-101 1.427532418032662 1106 1 0.11286721581258874 1106 2 0.0990021520772828 1106 3 0.06110086649368185 1106 4 0.04234932627809229 1106 5 0.02784602918158896 1106 6 -0.11049894415022266 1106 7 -0.058293010500854336 1106 8 0.005818182555209717 1106 9 0.053921145429789874 1106 10-11 -0.09151228286600599 1106 12-13 -0.05190696372678616 1106 14-15 -0.09335932264030333 1106 16-17 -0.050422941735305926 1106 18-19 -0.04665698386428829 1106 20-21 0.02462645150664855 1106 22-23 0.15177158993837025 1106 24-25 0.05320668064921108 1106 26-27 0.027927590562143223 1106 28-29 0.024056394339311282 1106 30-31 0.11595866613996719 1106 32-33 0.08420472864865047 1106 34-35 0.034145075114665246 1106 36-37 -0.0051355874226146625 1106 38-39 0.007179944332520449 1106 40-41 0.0551464431486437 1106 42-43 0.09599993380531657 1106 44-45 -0.05049729602648512 1106 46-47 -0.07452706901514716 1106 48-49 -0.030932288266555474 1106 50-51 -0.020426496383507242 1106 52-53 0.003629824508124102 1106 54-55 0.012391251204597609 1106 56-57 -0.009845509342873981 1106 58-59 -0.11059836810410673 1106 60-61 0.11917171725816189 1106 62-63 0.07574897098545108 1106 64-65 -0.07163740250722128 1106 66-67 -0.05873335428632487 1106 68-69 0.04078787525016736 1106 70-71 0.04227182257292128 1106 72-73 0.122768762654907 1106 74-75 0.20400814710751547 1106 76-77 9.494271342198601E-4 1106 78-79 0.16140760748847427 1106 80-81 0.21724429556524782 1106 82-83 0.4062284733047399 1106 84-85 0.41371588042643737 1106 86-87 0.37062395825615724 1106 88-89 0.3470802095208647 1106 90-91 0.36742345758413464 1106 92-93 0.35942244927471023 1106 94-95 0.23274008056698392 1106 96-97 0.43702196210549715 1106 98-99 0.9296601747796807 1106 100-101 0.9961574136616882 1107 1 0.23742259902786955 1107 2 0.1239679180008828 1107 3 0.006075988815521072 1107 4 0.0016076348763789383 1107 5 -0.005969746006435628 1107 6 -0.007520436499106609 1107 7 0.027108924867093265 1107 8 0.051580259621786695 1107 9 0.047510254523913886 1107 10-11 -0.008018618960129231 1107 12-13 0.07987852800118134 1107 14-15 0.03577382029747156 1107 16-17 0.03093482872988318 1107 18-19 -0.001434558264591601 1107 20-21 0.10039987413141205 1107 22-23 0.212966158157748 1107 24-25 0.1881500829640217 1107 26-27 0.122499051371598 1107 28-29 0.10136057845061686 1107 30-31 0.046640956413980916 1107 32-33 0.12082204663294505 1107 34-35 0.20043690495563027 1107 36-37 0.2547578054931705 1107 38-39 0.2669529754062552 1107 40-41 0.219070102924789 1107 42-43 0.19789494653772977 1107 44-45 0.20991689143392733 1107 46-47 0.1724897658842437 1107 48-49 0.1270496814874491 1107 50-51 0.1155092146623744 1107 52-53 0.13986920210238196 1107 54-55 0.2010047910187538 1107 56-57 0.18705486969107454 1107 58-59 0.13558246021865727 1107 60-61 0.20093212857852194 1107 62-63 0.2407425192542334 1107 64-65 0.23478871974462834 1107 66-67 0.148497793109577 1107 68-69 0.22633257364259407 1107 70-71 0.2133883116377575 1107 72-73 0.1574646163415636 1107 74-75 0.2893588953950541 1107 76-77 0.16440998473383317 1107 78-79 0.20966339685802993 1107 80-81 0.30220724318511927 1107 82-83 0.40363765836716325 1107 84-85 0.39964495019048485 1107 86-87 0.39374715051522813 1107 88-89 0.29432307652900747 1107 90-91 0.4413870923530574 1107 92-93 0.4764987705435004 1107 94-95 0.3975152881080959 1107 96-97 0.412213402627426 1107 98-99 0.9058075841498052 1107 100-101 1.0123968058367687 1108 1 0.05735086105946863 1108 2 -0.030781830663428877 1108 3 -0.08193708985265502 1108 4 -0.053707540510195884 1108 5 0.01626338296356522 1108 6 -0.02730829899041254 1108 7 -0.08574035207947617 1108 8 0.04093107492918335 1108 9 -0.005038290980529325 1108 10-11 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-1.269391753703914 2316 94-95 -1.2862137670876237 2316 96-97 -1.453577720674783 2316 98-99 -1.1689472248963995 2316 100-101 -1.4826648224784762 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135.0 3 77.0 4 219.0 5 584.0 6 1153.0 7 2022.0 8 3429.0 9 5340.0 10 7460.0 11 9581.0 12 11951.0 13 14646.0 14 16829.0 15 19417.0 16 22616.0 17 26393.0 18 30720.0 19 35087.0 20 38078.0 21 39917.0 22 44686.0 23 53073.0 24 66230.0 25 83061.0 26 105595.0 27 134432.0 28 169118.0 29 208407.0 30 254270.0 31 309037.0 32 376696.0 33 467343.0 34 603498.0 35 840220.0 36 1081665.0 37 1498407.0 38 1324620.0 39 268100.0 40 418.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.291581377533955 31.387336129077 27.71640935548984 22.604673137899205 2 23.02499348586402 28.94911389400981 27.943771690849506 20.082120929276666 3 21.088967806100168 28.229782018048745 28.487582772342872 22.193667403508215 4 21.70419981053192 28.795209080375177 28.141404745314187 21.359186363778715 5 22.459405028311917 28.558293604982243 27.981106549621 21.001194817084844 6 22.1384117119955 28.415742810744355 27.80300996082341 21.642835516436733 7 22.343280151329004 28.770872665470836 27.68196296119142 21.20388422200874 8 21.5292744659326 28.53315114141119 28.08700928371417 21.850565108942043 9 21.305600498720292 28.35256911657954 28.561865758022304 21.779964626677867 10-11 21.471950593757047 28.895572806871016 27.890797415650486 21.741679183721455 12-13 21.471216082147844 28.931993643671255 27.744818356529365 21.851971917651536 14-15 21.630326147191337 28.95161556688886 27.53954661613573 21.878511669784075 16-17 21.828120564536675 28.778052159963025 27.47181136424232 21.92201591125798 18-19 21.80364631383372 28.787210322013028 27.458149120386683 21.950994243766566 20-21 21.92776354896022 28.718869997349692 27.46186187229491 21.891504581395175 22-23 21.97548150751552 28.732465404845787 27.43214463838263 21.859908449256057 24-25 21.84714595212202 28.709338640875988 27.403031122278588 22.0404842847234 26-27 21.98987708765953 28.596040748545022 27.543526993923194 21.870555169872254 28-29 22.0269833410151 28.67927332828284 27.449825623377695 21.843917707324366 30-31 21.98468443180255 28.68006613221072 27.401780827959175 21.933468608027557 32-33 22.001161291640603 28.726756199768833 27.304739722590387 21.96734278600018 34-35 22.004865030542383 28.75484565617511 27.30884197189527 21.93144734138724 36-37 22.010408959769507 28.753020369696188 27.28215293028463 21.954417740249674 38-39 22.025208338481495 28.752416985730395 27.276541824648987 21.945832851139123 40-41 22.00785315142658 28.820890796983424 27.26441463230237 21.906841419287627 42-43 22.032002977089405 28.816405113165732 27.224770616949666 21.9268212927952 44-45 21.997134005197154 28.851360956068856 27.203261029394675 21.948244009339316 46-47 22.039554815636947 28.868741766448018 27.175443902228956 21.91625951568608 48-49 22.044133594588804 28.866188622663653 27.16816061534667 21.92151716740088 50-51 22.058409371846682 28.868348240894708 27.155995257115062 21.91724713014355 52-53 22.027135545161237 28.946804169219924 27.09812488120728 21.927935404411556 54-55 22.053258220943274 28.94965807697568 27.037065537305686 21.96001816477536 56-57 22.07790078893882 28.97586587202105 27.015797921367664 21.930435417672467 58-59 22.02873046995063 29.074543186384116 27.011021267863267 21.885705075801994 60-61 22.01367675044784 29.049176469422488 27.013175498357427 21.923971281772243 62-63 22.021384123659825 29.104189266100867 26.97199744694568 21.902429163293636 64-65 22.01370965093048 29.171197291857975 26.94802305301983 21.867070004191714 66-67 21.985277296943526 29.195474924918496 26.93723125103218 21.8820165271058 68-69 21.973355516680325 29.224110933046255 26.94461911111655 21.85791443915687 70-71 21.991564977388776 29.326405011859936 26.863206163703985 21.818823847047305 72-73 21.975670399932664 29.289524589690625 26.8508697496818 21.88393526069491 74-75 22.03744640246625 29.32458849939139 26.830500284426286 21.807464813716077 76-77 21.977536342560043 29.392474937162085 26.827339756350415 21.80264896392746 78-79 21.966740166504074 29.391167749488968 26.81210210737933 21.829989976627626 80-81 21.931901728666308 29.449740718403916 26.7831527516634 21.835204801266375 82-83 21.985261456294563 29.51182412201877 26.72395073907414 21.778963682612524 84-85 21.9173346463556 29.552958734605372 26.754349641110647 21.775356977928382 86-87 21.89078064072177 29.59831179753804 26.716660295129596 21.794247266610597 88-89 21.89290530867678 29.6532570409236 26.700365715953616 21.753471934446004 90-91 21.865986204942608 29.649550101202532 26.671257801519232 21.813205892335624 92-93 21.887753781968577 29.681383257649003 26.651618492262475 21.779244468119945 94-95 21.853308761666142 29.79752596365593 26.62564497415149 21.723520300526438 96-97 21.856935512392184 29.795442419251263 26.589812533484686 21.75780953487187 98-99 21.86868870720865 29.81667781295342 26.53992162098411 21.77471185885382 100-101 21.909145563428194 29.802634786122802 26.4997417889782 21.788477861470813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 131272.0 1 75496.5 2 14687.0 3 9037.0 4 7780.5 5 6714.0 6 6159.0 7 6097.0 8 5924.0 9 5436.5 10 5214.0 11 5722.5 12 8922.5 13 8843.0 14 6120.0 15 6347.0 16 6679.0 17 7560.5 18 9310.0 19 10931.5 20 13016.0 21 16439.0 22 20645.0 23 26345.0 24 32570.5 25 39776.0 26 50800.0 27 62969.0 28 71974.0 29 81004.5 30 95228.5 31 112583.5 32 130093.0 33 148091.0 34 166137.5 35 182531.5 36 198794.0 37 216273.5 38 229346.0 39 235263.0 40 244342.0 41 255133.5 42 261678.0 43 273064.0 44 286529.5 45 295906.0 46 299183.5 47 297925.5 48 300493.5 49 303451.5 50 303475.5 51 299878.5 52 295588.0 53 286763.0 54 267624.5 55 247422.5 56 225515.0 57 203698.0 58 187680.5 59 164545.0 60 133781.5 61 104605.0 62 76808.0 63 54415.5 64 37732.0 65 24270.5 66 14679.5 67 9165.0 68 5310.0 69 2620.5 70 1252.0 71 588.5 72 325.5 73 193.0 74 113.5 75 62.5 76 44.5 77 41.0 78 27.0 79 17.5 80 14.0 81 11.0 82 11.0 83 10.5 84 8.5 85 6.0 86 3.0 87 4.0 88 4.5 89 2.5 90 2.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15996026682879627 2 0.0 3 0.0 4 8.073858680560229E-4 5 6.972877951392924E-4 6 6.116559606485022E-5 7 2.8136174189831095E-4 8 0.0 9 9.786495370376034E-5 10-11 2.0184646701400572E-4 12-13 0.0 14-15 0.0 16-17 4.2815917245395146E-5 18-19 6.1165596064850215E-6 20-21 6.1165596064850215E-6 22-23 5.74956603009592E-4 24-25 0.0 26-27 6.116559606485022E-5 28-29 0.0050461616753501425 30-31 0.0036515860850715575 32-33 1.4068087094915548E-4 34-35 8.502017853014179E-4 36-37 4.1592605324098145E-4 38-39 0.0011254469675932438 40-41 0.00283808365740905 42-43 0.0012600112789359144 44-45 6.850546759263223E-4 46-47 0.008049392442134288 48-49 0.008147257395838048 50-51 0.0073643377662079654 52-53 0.014123136131373915 54-55 0.0057250997916699796 56-57 8.37968666088448E-4 58-59 0.0013150603153942795 60-61 6.1165596064850215E-6 62-63 9.174839409727531E-5 64-65 8.502017853014179E-4 66-67 9.786495370376035E-4 68-69 0.0028136174189831095 70-71 0.0019511825144687216 72-73 0.005553836122688399 74-75 0.0022019614583346074 76-77 0.0015474895804407105 78-79 0.0016575876533574407 80-81 2.4466238425940087E-4 82-83 0.0022141945775475775 84-85 6.116559606485022E-5 86-87 0.0018961334780103565 88-89 2.0184646701400572E-4 90-91 9.847660966440883E-4 92-93 0.00142515838831101 94-95 9.113673813662682E-4 96-97 0.0010214654542829984 98-99 0.5293515345836396 100-101 1.7738022858806562E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8174530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.30230038107389 #Duplication Level Percentage of deduplicated Percentage of total 1 91.38595145371688 58.7632690098714 2 4.566648186634713 5.8729196686334335 3 1.3099391600062404 2.526963040429587 4 0.6330777519007152 1.6283342306917907 5 0.36432400318896263 1.1713435744546 6 0.24434554413782475 0.9427188345556414 7 0.15587884907402166 0.7016358003349676 8 0.11524700604012743 0.592851808032937 9 0.09818000372423565 0.5681880081801679 >10 1.0107853094055004 14.879251415686548 >50 0.10241099764300807 4.266713980405198 >100 0.012797308928389324 1.0570859857708996 >500 0.0 0.0 >1k 1.3814186646961474E-4 0.2845173585675917 >5k 1.3814186646961474E-4 0.6203775311383267 >10k+ 1.3814186646961474E-4 6.123829753246884 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121491 1.4862138863029435 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16389 0.20048859078136602 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8612 0.10535162266209801 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2233119212970043E-5 0.0 0.0 0.0 0.0 2 4.893247685188017E-5 0.0 0.0 1.2233119212970043E-5 1.2233119212970043E-5 3 9.786495370376034E-5 0.0 0.0 1.2233119212970043E-5 1.2233119212970043E-5 4 1.3456431134267047E-4 0.0 0.0 2.4466238425940086E-5 1.2233119212970043E-5 5 1.3456431134267047E-4 0.0 0.0 2.4466238425940086E-5 4.893247685188017E-5 6 1.5903054976861055E-4 0.0 0.0 3.669935763891013E-5 9.786495370376034E-5 7 1.5903054976861055E-4 0.0 0.0 4.893247685188017E-5 1.3456431134267047E-4 8 1.7126366898158058E-4 0.0 0.0 4.893247685188017E-5 1.467974305556405E-4 9 2.324292650464308E-4 0.0 0.0 4.893247685188017E-5 1.467974305556405E-4 10-11 2.8747830150479604E-4 0.0 0.0 5.50490364583652E-5 1.7126366898158058E-4 12-13 3.731101359955863E-4 0.0 0.0 6.116559606485022E-5 1.8349678819455062E-4 14-15 4.403922916669215E-4 0.0 0.0 7.339871527782025E-5 2.2631270543994577E-4 16-17 4.954413281252867E-4 0.0 0.0 8.563183449079029E-5 2.691286226853409E-4 18-19 6.055394010420171E-4 6.1165596064850215E-6 0.0 9.174839409727532E-5 2.93594861111281E-4 20-21 6.544718778938972E-4 1.2233119212970043E-5 0.0 9.786495370376034E-5 3.058279803242511E-4 22-23 6.850546759263223E-4 1.2233119212970043E-5 0.0 9.786495370376034E-5 3.058279803242511E-4 24-25 7.401037123846876E-4 1.2233119212970043E-5 0.0 1.0398151331024536E-4 3.1194453993073606E-4 26-27 7.706865104171127E-4 1.2233119212970043E-5 0.0 1.1009807291673038E-4 3.241776591437061E-4 28-29 8.012693084495377E-4 1.2233119212970043E-5 0.0 1.1009807291673038E-4 3.3029421875019116E-4 30-31 8.257355468754778E-4 1.2233119212970043E-5 0.0 1.1009807291673038E-4 3.4252733796316117E-4 32-33 9.174839409727532E-4 1.2233119212970043E-5 0.0 1.162146325232154E-4 3.5476045717613123E-4 34-35 9.969992158570585E-4 1.2233119212970043E-5 0.0 1.2233119212970043E-4 3.608770167826162E-4 36-37 0.0010826310503478489 1.2233119212970043E-5 0.0 1.2233119212970043E-4 3.792266956020713E-4 38-39 0.0011866125636580942 1.2233119212970043E-5 0.0 1.2233119212970043E-4 3.792266956020713E-4 40-41 0.0012538947193294293 1.2233119212970043E-5 0.0 1.2233119212970043E-4 3.8534325520855634E-4 42-43 0.0013150603153942795 1.2233119212970043E-5 0.0 1.2233119212970043E-4 4.220426128474665E-4 44-45 0.0014251583883110098 1.2233119212970043E-5 0.0 1.2233119212970043E-4 4.893247685188017E-4 46-47 0.0015046736631953153 1.2233119212970043E-5 0.0 1.2233119212970043E-4 5.443738049771669E-4 48-49 0.0015903054976861056 1.2233119212970043E-5 0.0 1.2233119212970043E-4 5.50490364583652E-4 50-51 0.0016453545341444708 1.2233119212970043E-5 0.0 1.3456431134267047E-4 5.74956603009592E-4 52-53 0.001712636689815806 1.2233119212970043E-5 0.0 1.3456431134267047E-4 6.116559606485022E-4 54-55 0.0017860354050936262 1.2233119212970043E-5 0.0 1.3456431134267047E-4 6.116559606485022E-4 56-57 0.0018839003587973865 1.2233119212970043E-5 0.0 1.3456431134267047E-4 6.300056394679572E-4 58-59 0.002000114991320602 1.2233119212970043E-5 0.0 1.4068087094915548E-4 6.728215567133524E-4 60-61 0.0021469124218762422 1.2233119212970043E-5 0.0 1.467974305556405E-4 6.972877951392924E-4 62-63 0.0022508939351864876 1.2233119212970043E-5 0.0 1.5903054976861055E-4 7.034043547457774E-4 64-65 0.0024221576041680683 1.2233119212970043E-5 0.0 1.5903054976861055E-4 7.156374739587476E-4 66-67 0.0026240040711820742 1.2233119212970043E-5 0.0 1.5903054976861055E-4 7.278705931717175E-4 68-69 0.00282585053819608 1.2233119212970043E-5 0.0 1.5903054976861055E-4 7.462202719911726E-4 70-71 0.0030215804456036003 1.2233119212970043E-5 0.0 1.5903054976861055E-4 7.768030700235978E-4 72-73 0.003150028197339786 1.2233119212970043E-5 0.0 1.6514710937509555E-4 8.135024276625079E-4 74-75 0.0033763409027797314 1.2233119212970043E-5 0.0 1.7126366898158058E-4 8.44085225694933E-4 76-77 0.0036210032870391323 1.2233119212970043E-5 0.0 1.7126366898158058E-4 8.44085225694933E-4 78-79 0.003951297505789324 1.2233119212970043E-5 0.0 1.7126366898158058E-4 8.624349045143879E-4 80-81 0.004318291082178425 1.2233119212970043E-5 0.0 1.7738022858806562E-4 9.297170601857232E-4 82-83 0.004758683373845347 1.2233119212970043E-5 0.0 1.8349678819455062E-4 9.786495370376035E-4 84-85 0.005480437407410579 1.2233119212970043E-5 0.0 1.8349678819455062E-4 9.969992158570585E-4 86-87 0.006446853825235212 1.2233119212970043E-5 0.0 1.8349678819455062E-4 0.0010031157754635434 88-89 0.007609000150467367 1.2233119212970043E-5 0.0 1.8349678819455062E-4 0.0010214654542829984 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 375 0.0 29.119104 3 CAACGCA 5835 0.0 20.585108 12-13 ATCAACG 6115 0.0 19.953089 10-11 CGTACGC 295 0.0 19.31236 20-21 CGCGTAG 285 0.0 19.158476 54-55 CGTAGCG 305 0.0 18.679682 56-57 ACGTACG 330 0.0 17.983418 20-21 CTCGCGT 330 0.0 17.26635 52-53 TCAACGC 5775 0.0 17.05845 10-11 GGTATCA 7405 0.0 17.05415 8 AGTGGTA 7545 0.0 16.737907 5 TCGCGTA 345 0.0 16.51564 52-53 ACTCGCG 350 0.0 16.28035 50-51 AACGCAG 6150 0.0 16.134096 12-13 GTATCAA 7980 0.0 16.063284 9 CGCTAGT 375 0.0 15.193318 90-91 ACGCAGA 7980 0.0 15.141133 14-15 TGGTATC 8645 0.0 14.607978 7 AAGCAGT 9445 0.0 14.398528 1 AGAGTAC 8530 0.0 13.970141 18-19 >>END_MODULE